1.Application of Digestive Endoscopy in Diagnosis of Helicobacter Pylori Infection.
Yue-Fei YIN ; Yun LI ; Ya-Li CHEN ; De-Kui ZHANG
Acta Academiae Medicinae Sinicae 2018;40(4):563-567
Helicobacter pylori (Hp),a proven pathogen of digestive disease,is closely associated with gastrointestinal diseases including peptic ulcer,chronic active gastritis,stomach cancer,and stomach-associated lymphoid tissue lymphoma. Thus,precise and timely diagnosis of Hp is of great significance. In addition to C or C breath test,rapid urease test,and other commonly used Hp diagnosis methods,some new endoscopic techniques such as magnifying endoscopy,narrow-band imaging,confocal laser endomicroscopy,Fuji intelligent chromoendoscopy,and I scanning have been used for the direct observation of the fine-structure of stomach with Hp infection or for the identification of living Hp. This article reviews the application of digestive endoscopy in the diagnosis of Hp infection.
2.Isolation, expression analysis of a chilling induced cDNA from rice root with differential display: an evidence role for caffeine-sensitive calcium signal.
Kui-De YIN ; Xing-Mei ZHANG ; Shi-Qiang LIU ; Le-Gong LI
Chinese Journal of Biotechnology 2002;18(4):468-471
Chilling-sensitive rice varieties acquire chilling tolerance when their roots are exposed to water stress for short time. Caffeine-sensitive calcium signal was involved in this procedure. By using total RNA differential display, a chilling induced cDNA(ICT: induction of chilling treatment) was isolated from roots of chilling-sensitive rice variety. It was determined that it is a novel cDNA by homology searching. The transcript level of ict mRNA is up-regulated under chilling stress, it is decreased to low level when the samples were transferred to standard culture conditions. Pre-treated with mannitol for two hours is beneficial to inducing ICT level of expression. This chilling induction was inhibited by caffeine, suggesting that it may play a putative role in signal transduction of caffeine-sensitive calcium.
Cold Temperature
;
DNA, Complementary
;
genetics
;
isolation & purification
;
Gene Expression Profiling
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Gene Expression Regulation, Plant
;
drug effects
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Mannitol
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pharmacology
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Oryza
;
drug effects
;
genetics
;
Plant Roots
;
drug effects
;
genetics
;
RNA, Messenger
;
drug effects
;
genetics
;
metabolism

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