Arrhythmias, Cardiac ; complications ; genetics ; physiopathology ; Atrial Fibrillation ; complications ; genetics ; physiopathology ; Electrophysiology ; Humans

Background and purpose:Recently, a large number of researches have shown that cruciferous plants have the chemopreventive effect on tumor. Mechanisms of antitumorigenesis were investigated on antioxidation, antimutation, immunity and inducing apoptosis, and so on. Mustard seeds (MS) are the seeds belong to the cruciferous plants. This study aimed to investigate antioxidation and immune deviation of MS on colorectal tumor in rats induced by 1, 2-dimethylhydrazine (DMH). Methods:A total of 48 male Wistar rats were randomly divided into four groups:DMH alone, DMH+5%MS, DMH+7.5%MS, and the untreated control group(Saline). Colorectal tumorigenesis was induced by intraperitoneal injecting 30 mg/kg DMH once a week for 20 weeks. At the end of 32 weeks, the rats were sacrificed, then colorectal tumor incidence was observed and histological type was determined by HE staining. A colorimetric assay was used to detect levels of the lipid peroxidation product malondialdehyde (MDA) and the activity of antioxidant enzymes in the serum of all rats. The levels of Th1 and Th2 cytokines were detected with Luminex200. Results: No tumorous lesion was found in the untreated control group. However, the total tumor incidence in DMH+5%MS group and DMH+7.5%MS group was signiifcantly decreased 33.3%and 58.3%respectively, compared with the DMH group’s (100%, P<0.05). As DMH induced colorectal tumorigenesis, MDA and Th2 cytokines in the serum were signiifcantly higher in the DMH group than those in the untreated control group (P<0.05), but the activities of antioxidant enzymes were signiifcantly lower (P<0.05). While the MS treatment, compared with the DMH group, signiifcantly suppressed the MDA level but enhanced the activities of antioxidant enzymes and levels of Th1 cytokines (P<0.05). Conclusion: MS significantly decrease prevalence rates of DMH-induced colorectal tumor in rats. The mechanism may be related with the antioxidation and immune balance deviation.

Adrenergic beta-Antagonists ; Arrhythmias, Cardiac ; Humans

Electrocardiography ; methods ; Heart Conduction System ; physiopathology ; Heart Diseases ; diagnosis ; Humans

Objective: To investigate the chemical constituents of Croton crassifolius. Methods: A number of methods, such as extraction, chromatography on silica gel, and recrystallization, were utilized to isolate and purify the ethanol extract from C. crassifolius, and the structures of the compounds isolated were identified by physicochemical properties and spectral analyses. Results: Ten compounds were isolated and identified as cyperenoic acid (1), β-sitosterol (2), lupeol (3), chettaphanin II (4), 9-[2-(2(5H)-furanone-4-yl) ethyl]-4, 8, 9-trimethyl-1, 2, 3, 4, 5, 6, 7, 8-octahydro-naphthalene-4-carboxylic acid (5), acetyl aleuritolic acid (6), epitaraxerol (7), 9-[2-(2(5H)-furanone-4-yl) ethyl]-4, 8, 9-trimethyl-1, 2, 3, 4, 5, 6, 7, 8-octahydronaphthalene-4-carboxylic ester (8), teucvidin (9), and stigmasterol (10). Conclusion: Compounds 5 and 8 are newly-discovered compounds, named as crassifolius acid and crassifolius ester, and compounds 4, 6, and 7 are isolated from this plant for the first time.

Colorectal cancer is one of the most commonly seen gastrointestinal carcinomas and its pathogenesis has not yet been fully clarified. It is considered as a multi-step and multi-stage disease. Wnt signaling transduction pathway regulates cell growth,motility and differentiation,and plays a crucial role in the regulation of embryonic development and tumor genesis. This article reviewed the advances in study on Wnt signaling transduction pathway and colorectal cancer.

Objective To discuss the clinical features diagnosis and treatment of cerebral schistosomiasis. Methods A to?tal of 166 patients with cerebral schistosomiasis were treated and their clinical data were collected and analyzed retrospectively. Results In 166 cases of cerebral schistosomiasis the confirmative diagnoses of 156 cases were diagnosed according to the clin?ical manifestation etiology immunology and auxiliary examination CT MRI .In among 74 cases were confirmed by pathologi?cal examination 10 cases were diagnosed through to the diagnostic treatment. Totally 102 patients received the oral medication of praziquantel and they all improved and discharged 14?16 days later 64 patients received the craniotomy and praziquantel medication after the operation and 48 patients significantly improved others did not improve or aggravated. There was no opera?tive mortality. Conclusions Neuroimaging and laboratory tests are valuable in the diagnosis of cerebral schistosomiasis. The praziquantel treatment is selected firstly when the diagnosis was established. However in the case of serious intracranial hyper?tension intractable epilepsy and praziquantel treatment fails the surgical treatment is required.

Objective To establish lung function reference values and prediction equations in children and adolescents of the Han nationality in Heilongjiang province.Methods A survey in 588 healthy children and adolescents (292 males,296 females)aged 10 to 18 years old was carried out in Heilongjiang province in 2008.Eight flowvolume tests parameters were measured.Stepwise multiple regression was carried out to establish prediction equations for the parameters mentioned above.Results FVC and FEV1,were found,regardless of sex,to tend to go up with the increase of age(P ＜0.001).Beginning from the age of 14,male FVC and FEV1 became significantly higher than those of the female(P ＜0.001),the period of a fast increase of the male FVC and FEV1 taking place during the age of 13～14,while that of the female taking place in the age of 12 ～ 13,one age bracket earlier than the male.All lung volumes and flow rates,were found,regardless of sex,to tend to go up with the increase of age,height and weight(P ＜ 0.001).The regression equations of lung function were established.By comparison with the equations derived from our study and other authors' reports,it was found that the difference between measured data and predicted values from other authors was higher than those from our8.Conclusion Reference values and prediction equations for forced expiratory lung function applicable for children and adolescents of the Han nationality in Heilongiiang province were established.

Background and purpose:Most breast cancer cell lines were derived from malignant pleural fluid or other tissues that metastasized from breast carcinoma.Breast carcinogenesis is under represented due to the significant distinction of biological characteristics between cancer cell lines and primary breast cancers.Few primary breast tumors spontaneously developed into immortal cell lines.Overexpression of human telomerase reverse transcriptase(hTERT) in normal epithelial cells led to immortalization.We aimed to establish immortalized cultures derived from primary breast cancer by introducing hTERT and determine the gene profile of hTERT-transfected cultures.Methods:hTERT was introduced into breast tumor cultures with a puromycin-resistant retroviral construct,pBABE-hTERT-puro.Real-Time RT-PCR was used to analyze the expression level of hTERT after transfection.The upregulated gene profile was determined with microarray techniques.Results:hTERT was stably over expressed,at least 1 400-fold in hTERT-transfected cells compared to pBABE-puro transfected cultures.These ectopic over-expressions led to immortalization of transfected cultures,which could undergo at least 40-50 passages.Microarray analysis further confirmed the over-expression of hTERT in transfected cultures and demonstrated that 22 genes were up-regulated due to over-expression of hTERT.No down-regulated genes were observed cross all cultures.Conclusions:Over-expression of hTERT led to immortalization of breast tumor primary cultures and up-regulated certain genes.

AIM: The study is designed to probe for the relationship between waist to hip ratio(WHR)and static lung volumes of adults. METHODS: In July-October 2008, 1 307 healthy adults(372 males and 935 females)were selected in Heilongjiang province by means of questionnaire and physical examination. The height, weight, WHR, fat mass, percentage body fat and lung function were measured, and then grouped according to the standard of classification of WHR(central obesity male WHR ≥ 0.86, female ≥ 0.82)for analysis of the relationship between WHR and static lung volume. RESULTS: WHR was found, regardless of sex, to tend to go up with the increase in age and BMI(P<0.01), and both the fat mass and percentage body fat of the central obesity group were higher than those in the group with normal WHR(P<0.01). An independent negative correlation was found(P<0.05), also regardless of sex, between the WHR with expiratory reserve volume(ERV)in all these adults. ERV in central obesity group was lower than that in the group with normal WHR(P<0.05). Compared to the normal group, ERV in the central obesity group decreased by 11% for males and 8% for females(P<0.05). However, with regard to the relationship between WHR and VT, IC, MV, and VC, gender differences were found. For the males, a significant independent positive correlation was observed between WHR and IC(P<0.05), with IC of the central obesity group 6% higher than that in the group with normal WHR(P<0.05). For the females, significant independent positive correlation was found between WHR and MV(P<0.05), with the VT and MV of the central obesity group 7% and 6% higher(P<0.05), respectively, than that in the group with normal WHR. CONCLUSION: WHR is in an independent negative correlation with ERV. The elevation of WHR may play a role in the impairment of respiratory function. Its occurrence is accompanied by a rise of IC for the male and a rise of MV for the female. These changes in the two genders could be associated with the decrease in arterial oxygen tension caused by the decrease of ERV.