Objective To explore the feasibility and value of low dose CT scan for the diagnosis of pulmonary tuberculosis.Methods 150 patients with pulmonary tuberculosis underwent three doses CT scan (standard dose:150 mA;low-dose:15 mA and 30 mA) using GE dual slices helical CT.Besides the different tube current,other scan parameters including tube voltage,scan cycle,pitch,and collimation were the same in three dose groups.The scanned extent was from apexes to bases of lung.Image quality in standard group was compared with that in other two low dose groups and analyzed statistically by three radiologists.Results CT characteristics of pulmonary tuberculosis could be detected efficiently using low dose CT scan(30 mA) program,which was no significant as compared with the CT image using standard CT sose(P＞0.05).Howere,CT scan at 15 mA obviously affected on the diagnosis for both active and inactive pulmonary tuberculosis,the difference was significant (P＜0.05).Conclusion Low dose CT scan can replace totally the standard dose CT scan in diagnosing pulmonary tubercrulosis.

Objective To explore the technique and clinical value of percutaneous lung puncture and injection of drug under CT-guided in the treatment of multi-drug resistant cavitary pulmonary tuberculosis(MDR-TB).Methods 84 patients of positive sputum smear with MDR-TB were undergone injecting drugs into the pulmonary cavities by percutaneous lung puncture under CT-guided.The treatments were carried out by self-made locating mark which could confirm the appropriate puncture point,puncture angle and the puncture line.Results The operations of puncture in all cases were successful,the complications included pneumothorax in 3 cases,pulmonary hemorrhage in 1 case and subcutaneous emphysema in 1 case.After treatment,the mean rate of sputum negative,improvement of pulmonary cavity and cavity closure was 91.7%,89.3% and 66.7% respectively.Conclusion The percutaneous pulmonary puncture and injection of drug under CT-guided was a simple,visualized,right localization and less complications method in the treatment of the MDR-TB.

Animals ; Apigenin ; pharmacology ; Female ; Follicle Stimulating Hormone ; blood ; Gonadotropin-Releasing Hormone ; blood ; Luteinizing Hormone ; blood ; Phytoestrogens ; pharmacology ; Rats

Objective To explore the relationship between high altitude de-adaptation syndrome and training burnout of plateau-garrisoned soldiers after returning to the plain.Methods A total of 140 plateau-garrisoned soldiers who had returned to the plain were chosen by random cluster sampling and measured with the Training Burnout Test.They were divided into two groups according to the occurrence of acute mountain sickness.We compared the differences in training burnout between the two groups and analyzed the relationship between acute mountain sickness and training burnout. Results ①The incidence of high altitude de-adaptation syndrome of plateau-garrisoned soldiers was 80.00% after returning to the plain.There was statistically significant difference between plateau soldiers after returning to the plain,the plateau stability-keeping forces that returned to the plain (78.36%) (χ2 =0.188, P=0.664) and plateau migrants who returned to the plain (75.10%) (χ2 =1.279, P=0.258).Main symptoms of high altitude de-adaptation were fatigue (12.86%), dizziness (11.43%) and meakness (10.00%).②Compared with soldiers who had no high altitude de-adaptation syndrome, victims of de-adaptation syndrome had higher scores of training burnout, physical and psychological exhaustion and training-alienation (P<0.01).Compared with soldiers who had normal body mass indexes, those who were overweight had higher incidence of altitude de-adaptation (P<0.05).③The regression equation between plateau de-adaptation syndrome and training burnout of plateau soldiers after returning to the plain (P<0.01) is:Training burnout =0.498 ×plateau de-adaptation syndrome -0.126 ×age+0.038 ×length of military service +0.069 ×educational degree+0.029 ×body mass index.Conclusion There exist correlations between high altitude de-adaptation syndrome and training burnout of plateau soldiers after returning to the plain.Reducing the occurrence of high altitude de-adaptation syndrome can help reduce the degree of training burnout.

Objective To explore the relationship among psychological stress, emotion regulation type and job burnout of military stationed in Xinjiang,and provide theoretical basis for intervening job burn-out in military stationed in Xinjiang.Methods 757 soldiers were chosen by random cluster sampling meth-od and measured with the job burnout scale for military personnel,the psychological stress self-evaluation test and the questionnaire of the soldier’ s emotion regulation type.Results ①The scores of somatization,self-evaluation,passive work slowdown and job burnout of the only-child soldiers ( respectively ( 6.34 ±4.86 ) , (7.66±4.16),(5.00±3.51),(28.60±15.82)) were significantly higher than those of the non-only-child sol-diers(respectively(5.34±4.51),(6.89±4.28),(4.25±3.22),(25.73±15.04) ( t=2.804,2.384,2.950, 2.446;P<0.05 or P<0.01) .For the score of the three factors ( sense of achievement,somatization,and pas-sive work slowdown),as well as the total score of job burnout,soldiers who had injury history were signifi-cantly higher than those who had not ( t=3.471,3.676,4.222,3.469;P<0.01) .The total score and five fac-tors scores of job burnout among soldiers who had high expectation were significantly lower than those soldier who had normal or less expectation ( P<0.05 or P<0.01).② Psychological stress,cognition attention,self comfort,training expectation and emotional appeal had significant predictive effect on job burnout ( t=14. 518,8.241,-4.332,2.990,-2.695;P<0.01).③Cognition attention played a mediating role between psy-chological stress and job burnout,and the rate of mediating effect was 18.8%.Self comfort played a mediating role between psychological stress and job burnout,and the rate of mediating effect was 4.4%.Conclusion -Only-child or not,injury history and training expectation have important influence on job burnout.Cognition attention and self comfort play mediating role between psychological stress and job burnout.

Objective To evaluate the human resource in a national institute of parasitic diseases from 2007 to 2016，so as to provide a reference for the construction of a well⁃crafted human resource of national parasitic diseases control and prevention. Methods The basic information of the staff in the national institute of parasitic diseases was investigated and a related database was established to analyze the quantity and structure of the human resource allocation in 10 years through the annual statistics each year. Results The number of staff in the institute increased by 6.25% in 2016 compared with that in 2007，and 43.32% of the staff were under 35 years old. In 2016，59.36% of the staff had a master degree or a higher level degree，and 37.97% of the staff had senior technical titles. The difference value of the inflows and outflows was 3.21%. Conclusions The change of the structure and quality of human resource in this institute has a good tendency in the past ten years. The organization should optimize the human resource allocation and improve its capacity in disease control and prevention to broaden the methods of talent introduction and control the brain drain problem.

OBJECTIVETo investigate the proliferation inhibition and the differentiation effects of realgar (As4S4) nano-particles on human acute myeloid leukemia cell line HL-60.

METHODCell viability was determined by MTT and PI-stained cell cycle assays. The realgar induced morphological changes on cells were examined after Wright-Giemsa staining. The cell differentiation was evaluated with NBT and specific cell surface antigen (CD11b and CD14) expression assays.

RESULTHL-60 cells exhibited obvious morphological features of differentiation after the realgar treatment. A 24 h incubation of the cells with 0.25-1.0 micromol x L(-1) realgar caused a great increase in NBT reduction ability. The expressions of CD11b and CD14 were augmented in cells treated with 0.50 micromol x L(-1) realgar for 48 h, and cell cycles were arrested in G1 phase.

CONCLUSIONLow dose realgar induces differentiation in human acute myeloid leukemia cell line HL-60.

Antineoplastic Agents ; administration & dosage ; pharmacology ; Arsenicals ; administration & dosage ; pharmacology ; CD11b Antigen ; metabolism ; Cell Differentiation ; drug effects ; Cell Proliferation ; drug effects ; G1 Phase ; drug effects ; HL-60 Cells ; Humans ; Lipopolysaccharide Receptors ; metabolism ; Materia Medica ; administration & dosage ; pharmacology ; Nanoparticles ; Sulfides ; administration & dosage ; pharmacology

Objective: To explore the role of cytosine-phosphate-guanine oligodeoxynucleotide(CpG ODN)in enhan- cing the radiosensitivity to X-ray in mouse with Lewis lung cancer.Methods: The tumor-bearing mouse model was in- duced by injevting Lewis lung cancer cells into the right infra-axillary dermis.Thirty-two C57BL/6J mice were evenly ran- domized into 4 groups.Group A: the control group;Group B: the X-Ray radiation group;Group C: the CpG group; Group D: the CpG plus X-Ray radiation group.Group B was treated with X-Ray radiation only(3 Gy/F,on day 1,3,5, 8,10,and 12;the total dose was 18 Gy);group C was administered with CpG ODN 0.05 mg on day 1,3,5,8,10, and 12;group D was administered with CpG ODN 6h before X-ray radiation.The tumor growth and tumor growth delay (TGD)were observed in all groups.Meanwhile,the pathological change of the tumor tissue was observed with H-E staining method and the apoptosis of tumor cells were examined with the method of TUNEL.Results: The Lewis hmg cancer-bearing model was successfolly established in mice.The tumor volumes of the treatment groups were smaller than that in lhe control group(P

Objective To observe the effects of ( - )-epigallocatechin-3-gallate (EGCG) on human prostate cancer xenografted tumor growth and connexin43 expression in nude mice,and explore the mechanism of the EGCG on prevention for prostate cancer.Methods The methyl thiazolyl tetrazolium and annexin-V/PI double-labeled flow cytometry methods were used to observe the growth inhibiting rate (IR)and apoptosis rate (AR) of human prostate cancer cell line PC-3 which was treated by EGCG at different concentration (10,20 and 40 mg/L,respectively).The scrape-loading fluorescence dye transfer method was applied to assess the gap junction intercellular communication (GJIC) through fluorescence microscope.PC-3 cells were subcutaneously transplanted to establish tumor-bearing nude mice model.A total of 32 mice were randomly divided into four groups,both control group and three treatment groups were treated with different doses of EGCG ( 10,20 and 40 mg/kg,respectively).After two weeks,the mice prostate tumor tissues were taken out.The tumor wet weight was measured and tumor growth inhibiting rate was calculated.The tumor microvascular density (MVD) and apoptosis index (AI) were detected by the immunohistochemical techniques and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling techniques,respectively.Semi-quantitative reverse transcription polymerase chain reaction was used to examine the expression level of the Cx43 mRNA.Results EGCG at concentration ( 10 and 20 mg/L) could significantly inhibit the proliferation[(22.33 ±4.62)％,(38.67 ±5.67)％ vs (3.47 ±0.31 )％,P ＜0.01],induce the apoptosis [(7.84 ± 1.37 ) ％,( 24.53 ± 2.28 ) ％ vs ( 2.17 ± 0.70 ) ％,P ＜ 0.01] and enhance the GJIC of PC-3 cells.EGCG of different doses could inhibit prostate cancer xenografted tumor growth,induce tumor cells apoptosis and inhibit angiogenesis.EGCG ( 20 and 40 mg/kg) could effectively up-regulate Cx43 mRNA expression in xenografted tumor (0.58 ± 0.08,0.80 ± 0.07 vs 0.42 ± 0.04,P ＜ 0.0 ).The effects had significant correlation with the dose-dependent of EGCG ( P ＜ 0.05 ).Conclusions EGCG could up-regulate the Cx43 expression and enhance the gap junction intercellular communication mediated by Cx43 in the prostate tumor,which provide the experimental evidence for the mechanism of its effectively inhibiting the prostate cancer growth.

Objective To investigate the gene expression of PIWIL2 in the bladder urothelial carcinoma (BTCC) and siRNA interact on PIWIL2 gene expression in human bladder cancer cell line BIU-87.Methods Semi-quantitative reverse transcription polymerase chain reaction (qRT-PCR) was applied to detect the PIWIL2 expressions in tissues of BTCC (46 cases),cystitis glandularis(21 cases),adjacent non-cancerous tissues (17 cases) and normal bladder tissues (7 cases). 3 specific siRNA targeted PIWIL2 gene were synthesized after designed and transferred. After siRNA was transferred into BIU-87 cells, MTI and TUNEL methods were applied to detect the proliferation inhibitory rate (IR) and apoptosis index (AI) in BIU-87 cells,qRT-PCR and Western blot were used to examine effects of siRNA on the expressions of the PIWIL2 gene and protein,respectively.Results The expression rate of PIWIL2 mRNA in BTCC tissues was 76.08 ％(35/46) and significantly higher than those in the cystitis glandularis tissues (42.86 ％,9/21),adjacent non-cancerous tissues (41.17 ％,7/17) and normal tissues (7.14 ％,1/14) (P =0.008,P =0.010,P =0.000).The IR [(37.52±8.84) ％,(64.36±9.64)％] and (62.94±8.43) ％] and AI [(26.18±5.42) ％,(38.75±6.19) ％ and (40.02±5.64) ％] of BIU-87 cells in the siRNA 1～3 groups were respectively significantly higher than those [(1.97±0.02) ％ and (3.35±0.47) ％] in the control group(P=0.000),and expressions of PIWIL2 mRNA and protein in the siRNA groups were both lower than those in the control group. Moreover, the effects of siRNA 2 group and siRNA 3 group on inhibiting PIWIL2 expression, IR and AI of BIU-87 cells were stronger than siRNA 1 group. Conclusion The over-expression of PIWIL2 suggested that it played an important role in the mechanism of development and malignant progression of BTCC. The siRNA of transcription can significantly inhibit its expression, induce cell apoptosis and inhibit the growth of BIU-87 cells which might provide the experimental evidence for the gene targeting therapy of bladder tumor.