1.Clinical Correlation between Plasm Homocysteine Levels and Cerebral Infarction
Journal of Kunming Medical University 2014;(2):102-104
Objective To investigate the relationship between the plasma homocysteine (Hcy) and cerebral infarction.Methods 84 patients with cerebral infarction in our department were selected as study group and 56 cases from medical examination center were selected as control group. The fasting plasma homocysteine (Hcy) was determined in both groups . Result The level of Hcy in cerebral infarction group was significantly higher than the control group, there was a significant difference ( <0.01) .Conclusion High homocysteine levels is an important independent risk factor of cerebral infarction.
2.Unrelated umbilical cord blood stem cell transplantation in a patient with chronic myelocytic leukemia.
Yu-deng LIN ; Yi-kui SHEN ; Yong-kang LI
Chinese Journal of Pediatrics 2006;44(8):586-586
Blood Donors
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Child
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Cord Blood Stem Cell Transplantation
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adverse effects
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methods
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Female
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Follow-Up Studies
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Graft vs Host Disease
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prevention & control
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Humans
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Infant, Newborn
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Leukemia, Myelogenous, Chronic, BCR-ABL Positive
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surgery
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Transplantation, Homologous
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Treatment Outcome
3.Co-antitumor effect and hepatic protection of melatonin on advanced primary liver cancer treated by transcatheter arterial chemoembolization
Jianjun YAN ; Feng SHEN ; Kui WANG ; Mengchao WU
Academic Journal of Second Military Medical University 2001;0(09):-
Objective:To observe the co-antitumor effect and hepatic protection of melatonin on unresectable advanced primary liver cancer treated by transcatheter arterial chemoembolization (TACE). Methods:One hundred and forty patients with unresectable advanced primary liver cancer were divided into 2 groups at random, seventy patients received TACE alone while the other 70 patients were treated by TACE+Mel (20 mg/d at 8:00 P.M orally, 7 d before each TACE and lasted for 21 d). Results: The partly resolution rate of TACE and TACE+Mel were 12.86% and 22.86% respectively( P
4.Current treatment for intermediate-stage hepatocellular carcinoma
Wenxin WEI ; Zhengqing LEI ; Kui WANG ; Feng SHEN
Chinese Journal of Digestive Surgery 2016;15(4):407-410
Staging of hepatocellular carcinoma (HCC) need to consider tumor factors and functional status of liver,which is different from other solid tumors.Now there are many clinical staging and prognosis systems of HCC.Intermediate stage HCC is commonly occurred at disease diagnosis,with varied clinicopathological charactertics,therapeutic options and survival outcomes of patients.In this review,the definition,current therapeutic strategies,substaging model of intermediatestage HCC and the efficacy of liver resection for patients of intermediate-stage HCC are discussed for further improving feasibility of individualized surgical therapy.
6.To investigate the relationship between P15INK4B gene promoter methylation degree and different types of MDS and prognosis
Guoyu HU ; Kui TAN ; Chaohui YUAN ; Chanjuan SHEN ; Jing LUO ; Yuxia LIU
Journal of Chinese Physician 2015;17(9):1339-1342
Objective To determine the methylation level of P15INK4B gene promoter in different types of myelodysplastic syndromes (MDS) and its correlation with its prognosis.Methods Methylation frequency of the P15INK4B gene promoter in 44 cases of MDS were determined by methylation-specific polymerase chain reaction (PCR) and pyrosequencing,and its correlation with clinical classification and characteristics of MDS were statistically analyzed.Results Frequency of P15INK4B gene promoter methylation in myelodysplastic syndromes-refractory anemia with excess blasts Ⅱ (MDS-RAEB Ⅱ) patients was (46.89 ± 15.41) %,significandy higher than that in other types of MDS (P < 0.05),but no difference in promoter methylation frequency was detected among the other types of MDS (P > 0.05) ; frequency of P15INK4B gene promoter methylation was found to be correlated with decline in platelet upon diagnosis (t =9.02,P < 0.01),but showed no significant correlation with drop of hemoglobin or leukopenia (P >0.05).As for the correlation between P15INK4B gene promoter methylation and MDS risk stratification,no significant difference was detected between the low-risk and very low-risk groups (P > 0.05),but significant differences were detected among the medium-risk,high-risk,and very high-risk groups (P < 0.05).In addition,frequency of P15INK4B gene promoter methylation was (49.21 ± 8.78)% in MDS patients that developed leukemia in the following two year,significantly higher than that in MDS patients who didn't (19.64 ± 6.24) % (P < 0.05).Conclusions P15INK4B gene promoter methylation frequency is a valuable indicator of prognosis of MDS patients.
7.Altered functional connectivity of insula in schizophrenia using functional magnetic resonance imaging
Hua REN ; Yunting ZHANG ; Kui REN ; Quan ZHANG ; Jianhua SHEN ; Xinjun ZHANG
Chinese Journal of Behavioral Medicine and Brain Science 2013;(4):321-325
Objective To investigate the insula and its related network of schizophrenia patients and its correlation to behavior performance with functional magnetic resonance imaging.Methods Fourteen patients with paranoid schizophrenia and sixteen normal subjects received blood oxygenation level dependent functional magnetic resonance imaging(BOLD-fMRI) examinations based on resting state and digital 2-back working memory task.The fMRI data were processed and analyzed with SPM2,AFNI and Matlab software packages (thresholded at P =0.005,uncorrected).Medial prefrontal cortex (MPFC,BA10) was placed as region of interest (ROI).Results The functional connectivity positively or negatively correlated with MPFC was lower for patient group than that for control group in resting and task states.In resting state,the brain areas negatively correlated with MPFC mainly included the bilateral superior temporal gyrus (BA22),cuneus (BA 18/19),inferior parietal lobule (BA40),right parahippocampus for patient group and the bilateral insula/superior temporal gyrus(BA22),inferior parietal lobule(BA40),paracentral lobule,parahippocampus for control group.The brain areas positively correlated with MPFC mainly included the pineal gland areas for patient group and the left anterior nucleus thalamus,bilateral medial superior frontal gyrus (BA6),right inferior corpus callosum (BA34) and the cerebellar vermis for control group in resting state.In 2-back task,the brain areas negatively correlated with MPFC mainly included the right cerebellar hemisphere,left superior parietal lobule/precuneus (BA7),left superior frontal gyrus (BA6) for patient group and the left medial dorsal thalamus,bilateral lateral premotor area (BA6),left inferior parietal lobule/precuneus (BA7),right cerebellar hemisphere for control group.The accuracy of reaction was lower in patient group than that in control group((78.30 ± 8.76) % vs (89.89 ± 8.05) %,P =0.01) and the correlation between the accuracy and the left medial dorsal thalamus had statistical significance (r =-0.52,P =0.04).Conclusion The altered functional connectivitv of the bilateral insula and its related network indicates functional disintegration of the limbic system in patients with schizophrenia.Widespreadly reduced functional connectivity and the functional disconnection of left medial dorsal thalamus mav be one of the reasons for poor behavior performance in schizophrenia patients.
8.Relationship between platelet activating factor acetylhydrolase genetic polymorphism and platelet activation and prognosis in patients with ischemic stroke
Jianping ZHOU ; Yuxia SHEN ; Kui YIN ; Chen WANG ; Yuchen QIU ; Wanxiang WANG ; Jianjin DENG
Chinese Journal of Postgraduates of Medicine 2009;32(10):27-31
Objective To investigate the corelation between platelet activating factor acetylhydrolase (PAF-AH) genetic polymorphism and ischemic stroke. Methods The plasma PAF-AH genotype was determined in 205 patients with iachemic stroke and 114 normal subjects by the polymerase chain reaction. The levels of plasma platelet activating factor (PAF), platelet α-granule membrane glycoprotein-140(GMP-140), β-thromboglobulin (β-TG) and the levels of platelet factor 4 (PF4) were analyzed. Results The prevalence of the mutation genotype and plasma PAF, GMP-140, β-TG and PF4 in the patients with isehemic stroke [42.44%,(91.08 ± 39.10) ng/L, (36.46 ± 13.10) μg/L, (41.75 ± 11.18) μg/L, (29.05 ± 9.16) g/L, respectively] were significantly higher than those in the controls[21.05%,(64.30 ± 18.81) ng/L, (18.27 ± 7.68) μg/L, (30.94 ± 8.47) μg/L, (18.75 ± 6.06) μg/L](P< 0.01). The levels of plasma PAF, GMP-140 were significantly higher in mutation genotype patients than those in the normal genotype patients (P < 0.01). Conclusions The activation function of platelet in the acute phase of patients with ischemic stroke increases, and it is associated with genetic polymorphism of PAF-AH. The PAF-AH gene mutation may be a novel genetic marker for high risk of ischemic stroke.
9.Effects of Shenqi Bufei Tang Decoction on the Expression of HDAC2 and NF-κB p65 in Airway Smooth Muscle Tissues of COPD Rats with Lung-Qi Deficiency Syndrome
Guiying WU ; Kui ZHANG ; Ping YAN ; Xiangyan ZHANG ; Sha LUO ; Lili SHEN ; Zhu YANG
Herald of Medicine 2016;(3):224-228,229
Objective To examine the effects of Shenqi Bufei Tang decoction on the expression of histone deacetylase-2 ( HDAC2) and nuclear factor-κB p65 ( NF-κB p65) in the airway smooth muscle tissues of COPD rats with lung-qi deficiency syndrome. Methods A total of 40 male rats were randomly divided into normal control group,model control group,Shenqi Bufei Tang decoction group,and aminophylline group.The COPD rat model with lung-qi deficiency syndrome was established by intra-tracheal instillation of lipopolysaccharide (LPS) and passive smoking for 28 days.Pathological changes of lung tissues were ob-served under the light microscope and the thickness of the small airway wall and airway smooth muscle ( ASM) layer analyzed by the image analysis.Immunohistochemistry,real-time PCR and Western blotting were used to detect the expressions of NF-κB p65 and HDAC2 in ASM. Results The thickness of the airway wall and ASM,and the expression levels of NF-κB p65 mRNA and protein were significantly increased in the model control group when compared with those in the normal control group ( P<0.05) . They were significantly reduced and the expression levels of HDAC2 mRNA and protein were markedly increased in Shenqi Bufei Tang decoction group and aminophylline group,which were compared with those in the model group (P<0.05).There were no sig-nificant differences in these indices between Shenqi Bufei Tang decoction group and aminophylline group (P>0.05). Conclu-sion Shenqi Bufei Tang decoction can inhibit the proliferation of ASM in COPD rats with lung-qi deficiency syndrome,which may be associated with the increased expression of HDAC2 and decreased expression of NF-κB p65.
10.Analysis on the Antimicrobial Susceptibilities and the Prevalence of ESBLs Encoding Genes of Escherichia Coli Isolates Collected from Blood
Xuejing XU ; Xiaoli CAO ; Zhifeng ZHANG ; Mingzhe NING ; Wanqing ZHOU ; Kui ZHANG ; Han SHEN
Journal of Modern Laboratory Medicine 2016;(1):55-57
Objective To analyze the susceptibilities of Escherichia coli isolates collected from blood and the prevalence of ESBLs encoding genes.Methods A total of 121 Escherichia coli isolates collected from blood during 2012 were analyzed for antimicrobial susceptibilities by software of WHONET 5.6,the production of ESBLs was confirmed by confirmatory pheno-typic testing,PCR and DNA sequence were further implemented to analyze the ESBLs-encoding genes.Results 121 E.coli isolates displayed high resistance towards broad spectrum penicillin and 2nd or 3rd generation cephalosporins,levofloxacin and cotrimoxazole,with the resistance rates being more than 40%,susceptibilities to imipenem,piperacillin/tazobactam,ami-kacin were observed,with the resistance rates to be less than 12%,86(88.7%)out of 121 isolates were found to produce ESBLs.Among them,59.5% (72),38.8% (47)and 4.1% (5)were confirmed to carry blaCTX-M,blaTEM and blaSHV genes.Additionally,2(1.7%)isolates carried all the genes detected,30(24.8%)isolates carried both of blaCTX and bla-TEM,1(0.8%)isolate carried both of blaSHV andblaTEM.Conclusion Most of the E.coli isolates from the blood culture in Nanjing Gulou Hospital produce ESBLs,and displayed resistance towards most of the penicillins,cephalosporins and sin-gle amide antimicrobial agents should be chosen according to susceptibility results.