Adult ; Antigens, CD20 ; metabolism ; CD3 Complex ; metabolism ; Diagnosis, Differential ; Female ; Humans ; Hyperplasia ; immunology ; pathology ; Ki-1 Antigen ; metabolism ; Lymphoma ; immunology ; pathology ; Lymphoma, B-Cell ; immunology ; pathology ; Uterine Cervical Neoplasms ; immunology ; pathology

By non-steady method,the effective diffusivity of ferrous sulphate within alginate calcium gel entrapped without bacteria was measured.Meanwhile the oxidation ability of entrapped bacteria was analyzed.Experimental results showed that the effective diffusion coefficient of ferrous sulphate decreased with the increase of alginate concentration,the optimum alginate concentration is 2%(W/V).The effect of calcium chloride on the effective diffusivity was neglectable.The incubation of ferrooxidans would pass through 10 hours,and the diffusion coefficient within gel entrapped Thiobacillus ferrooxidans cells was less remarkably than that of ferrous sulphate without entrapped cells.For the entrapped cells,the absolute oxidation time was shortest and the rate change was fastest with the initial Fe concentration 5g/L.The absolute oxidation time was same when the initial Fe concentration was 8g/L and 10g/L.

Objective To evaluate the clinical curative effect of applying the modified Xiaojing Decoction, an experienced recipe prescribed by Professor LIU Zhen-Huan, for the treatment of attention deficit hyperactivity disorder(ADHD)children with liver-kidney yin deficiency by using the Swanson,Nolan,and Pelham,VersionⅣ(SNAP-Ⅳ) rating scale. Methods By the methods of randomized controlled trial, 60 cases of ADHD children were randomly divided into Chinese medicine group and control group,30 cases in each group. Chinese medicine group was treated with Xiaojing Decoction orally, and the control group was given Methylphenidate Tablets orally. After treatment for 4 weeks, the scores of subscales of SNAP-Ⅳ rating scale and clinical remission rate of the two groups were compared. Results(1)After treatment for 4 weeks,the scores of attention-deficit subscale and hyperactivity-impulsivity subscale of the two groups were much improved (P<0.01 compared with those before treatment),and the improvement in Chinese medicine group was superior to that in the control group(P<0.05).(2)Clinical remission rate of Chinese medicine group arrived to 60.0%,and that of the control group was 33.3%,the difference being significant(P < 0.05). Conclusion The modified Xiaojing Decoction,an experienced recipe prescribed by Professor LIU Zhen-Huan,exerts certain therapeutic effect for the treatment of ADHD children with liver-kidney yin deficiency.

OBJECTIVETo study the damages of fluoride on the male reproductive system in rat testes.

METHODSA total of 30 male SD rats were randomly divided into control group, high, low dose fluorine treated groups, which were given normal saline ,20 mg/kg sodium fluoride, and 10mg/kg sodium fluoride respectively. After 39 days the change of the weight of rats and the number of sperms were observed. The change of telomerase reverse transcriptase(TERT) and proliferating cell nuclear antigen (PCNA) were observed by using in situ hybridization and radioimmunoassay respectively.

RESULTSThe weight was (273.39 +/- 20.68), (240.00 +/- 21.39) g in NaF treated groups, which was lower than that in control group(P < 0.05); The rate of TERT expression in germ cells of testes in NaF treated groups was (13.89 +/- 4.86)% and (6.33 +/- 4.42)% respectively, which was significantly lower than that in control group (P < 0.05). The rate of PCNA expression in germ cells of tests in NaF treated groups was (0.71 +/- 0.05)%, (0.60 +/- 0.08)% respectively, which also was significant lower than that in control group(P < 0.05). The number of sperms was (18.31 +/- 1.20)10(10)/L, (9.17 +/- 1.38)10(10)/L, which was lower than that in control group (P < 0.05).

CONCLUSIONFluorine possibly damages the male reproductive system by reducing the expression of TERT and PCNA.

Animals ; Fluorides ; toxicity ; Germ Cells ; metabolism ; Male ; Proliferating Cell Nuclear Antigen ; metabolism ; Rats ; Rats, Sprague-Dawley ; Spermatozoa ; metabolism ; Telomerase ; metabolism ; Testis ; metabolism

OBJECTIVETo observe the pathological and clinical characters of nonalcoholic steatohepatitis (NASH).

METHODSLiver biopsy tissues taken from 97 patients negative for common viral detection with serological test and immunohistochemistry as well as in situ hybridization, were observed by routine light microscopic examination. And the clinical data of patients with NASH was analyzed.

RESULTSAmong the chronic hepatitis patients with unknown etiology, the detection rate of NASH was 15.5% (15/97). The characteristic lesions in NASH patients included macrovesicular steatosis in zone 3 of lobules, hepatocytes ballooning degeneration, lobules diffused with acute and chronic inflammation, and perisinusoidal fibrosis. Grading and staging according to Brunt's method, histological lesions could be accounted for G1S1 in 7 patients, G2S2 in 3 patients, G3S1 in 4 patients and G3S2 in 1 patient. There were 14 patients with mild to moderate elevation of transaminase, 10 with hyperlipemia, 8 with diabetes and 9 with fatty liver by ultrasonography.

CONCLUSIONNonalcoholic steatohepatitis is a common form of unknown etiology chronic liver disease with certain clinic-pathological features.

Adult ; Biopsy ; Fatty Liver ; pathology ; Female ; Humans ; Liver ; pathology ; Male ; Middle Aged

OBJECTIVETo evaluate the safety and effectiveness of magnesium sulphate for reducing the incidence of the emergence agitation in patients undergoing surgery for esophageal carcinoma with general anesthesia.

METHODSThis randomized, double-blind, controlled clinical trial was conducted in patients undergoing elective radical resection of esophageal carcinoma in Sichuan Provincial People's Hospital between October, 2015 and March, 2016. The patients underwent surgeries under general intravenous anesthesia, and after anesthesia induction, the patients were infused with magnesium sulphate at the rate of 0.1 mL·kg·h(2.5 g magnesium sulphate in 50 ml saline pumped at 5 mg·kg·h) or saline (control group) at the same speed until the end of the surgery. The Riker's sedation agitation scale, pain score, muscle tension score, extubation time, residence time in the postanesthesia care unit (PACU), and adverse reactions were recorded.

RESULTSA total of 108 patients were enrolled including 57 in magnesium sulphate group and 51 in the control group. Riker's sedation agitation scale in the PACU, pain score, and muscle tension score were all significantly lower but the extubation time was longer in magnesium sulphate group than in the control group (P<0.05). In all the patients, blood Calevel was reduced significantly after the operation; blood Mglevel was significantly lowered postoperatively in the control group, and the postoperative Mglevel was significantly higher in magnesium sulphate group than in the control group (P<0.05). Mglevel underwent no significant variations in magnesium sulphate group after the operation (P>0.05). The residence time in the PACU, postoperative Riker's sedation agitation scale score, and blood Klevel were all similar between the two groups(P>0.05).

CONCLUSIONInfusion of magnesium sulphate at 5 mg·kg·hcan significantly reduce the incidence of emergence agitation in patients undergoing esophageal carcinoma under general anesthesia without causing such complications such as muscle recovery delay or electrolyte disorder.

To study the effect of Yinghua Pinggan granule (YHPG) against influenza A/H1N1 virus in vivo and on the immunologic function of infected mice. The intranasal influenza virus infection was adopted in ICR mouse to establish the influenza virus pneumonia model. At the 3rd and 7th day after the infection, the lung index and pathologic changes in lung tissues of mice were detected. Realtime PCR and flow cytometry were employed to observe the virus load in lung tissues and the levels of CD4+, CD8+, and CD4+/CD8+ in peripheral blood. The result showed that at the 3rd and 7th day after the infection, YHPG (15, 30 g x kg(-1)) can significant decrease in the lung index and virus load in lung tissues of mice infected with influenza virus, alleviate the pathologic changes in lung tissues, significantly increase the levels of CD4+ and CD4+/CD8+ ratio and reduce the levels of CD8+ in whole blood. This indicated that YHPG can inhibit the influenza virus replication, alleviate pulmonary damage and adjust the weak immunologic function of infected mice, with a certain therapeutic effect on mice infected by H1N1 virus in vivo.
Animals ; Antiviral Agents ; administration & dosage ; Humans ; Influenza A Virus, H1N1 Subtype ; drug effects ; genetics ; physiology ; Influenza, Human ; drug therapy ; pathology ; virology ; Lung ; pathology ; virology ; Male ; Mice ; Mice, Inbred ICR ; Virus Replication ; drug effects

OBJECTIVETo study the effects of miR-124-1 on neuronal differentiation of rat bone marrow mesenchymal stem cells (MSCs).

METHODSMSCs cells were assigned into three groups: control (uninfected and untransfected), miR-124-1+ (infected with miR-124-1), and miR-124-1- (transfected with Anti-rno-miR-124* Inhibitor). MSCs were induced by β-mercaptoethanol (β-ME) to differentiate into neurons. The fluorescence expressed by infected MSCs was observed under an inverted fluorescence microscope. MTT method was used to measure cell survival rate after transfection or infection. Immunocytochemistry, RT-PCR and Western blot methods were used to detect the expression of β3 tubulin, MAP-2 and GFAP 6 days after β-ME induction.

RESULTSThe expression of miR-124-1 in the miR-124-1+ group was significantly higher 2 days after infection of lentivirus vector compared with the control group (P<0.01). In the miR-124-1- group, the cell survival rate and the miR-124-1 expression level decreased significantly 24 hrs after transfection of anti-rno-miR-124* inhibitor (P<0.01). After 6 days of β-ME induction, the protein and mRNA expression levels of β3 tubulin and MAP-2 in the miR-124-1+ group were much higher than the other two groups (P<0.01); while the expression levels of β3 tubulin and MAP-2 in the miR-124-1-group were lower than the control group (P<0.01). The expression of GFAP in the three groups was weak (<1%).

CONCLUSIONSmiR-124 might promote neuronal differentiation of rat MSCs.

Animals ; Bone Marrow Cells ; cytology ; Cell Differentiation ; Female ; Glial Fibrillary Acidic Protein ; analysis ; Male ; Mesenchymal Stromal Cells ; cytology ; MicroRNAs ; physiology ; Microtubule-Associated Proteins ; analysis ; Neurons ; cytology ; Rats ; Rats, Wistar ; Tubulin ; analysis

This paper introduces the pelvic inflammatory disease treatment characteristics of TCM Master Xia Guicheng.He emphasizes that comprehensive regulation is needed for pelvic inflammatory disease needs,and clearing heat and detoxifying, removing dampness and blood stasis are not the only methods for regulating internal disease resistance.For the acute stage,the treatment should be based on blood-heat of lower Jiao,clearing heat and detoxifying,removing blood stasis to stop pain should be used.For the chronic stage,reinforcing healthy qi should be taking as the root treatment which advocates menstrual cycle therapy under the regulation of heart-kidney-uterus axis,and heart-kidney interaction,removing blood stasis to stop pain should be used.

This paper expounds the concept and method of TCM Master Xia Guicheng diagnosis and treatment of dysmenor-rhea,which is summed up as six kinds of methods.Master Xia believes that the cause of dysmenorrhea is generally attributed to qi stagnation or blood stasis,and the general treatment usually performs from six aspects including stopping pain,dredging channels,calming the heart,regulating liver,warming meridian and spasmolysis,which have achieved remarkable effects. The treatment root is focus on regulating the activities of yin-yang,qi and blood of the menstrual cycle rhythm,and to treat dysmenorrhea by improving female's imbalance of the body environment through balancing yin and yang,smoothing qi and blood circulation.