1.Effect of Chinese Herbs on ?-Globin Gene Cluster Locus Control Hepersensitive site 2 Site Binding with Nucleoprotein
li-min, CHAI ; zhi-kui, WU ; xin-hua, ZHANG ; zhi-ang, LIU ; hui-guo, CAI
Journal of Applied Clinical Pediatrics 1993;0(03):-
Objective To explore molecular mechanism and the curative effect of Yisuishengxue powder and its function of the hepersensitive site 2 (HS2) in ?-globin gene cluster locus control region binding with nucleoprotein.Methods After 3 months treatment of Yisuishengxue powder, nucleoprotein was extracted from the morrow cell before and after treatment. The HS2 DNA probes was combined with nucleoproteins.Electrophoresis gel mobile lag was utilized for observing the mobile velocity of DNA segment.Observe the mobile velocity of DNA probes.Results The mobile velocity of probes combined with nucleoproteins before treatment was different form that of the controls, while it was very close to the controls after treatment.Conclusions It is suggested that this compounding medicine might affect the DNA segment of HS2 site in ?-LCR binding with nucleoprotein GATA-1, which may be one molecular mechanism of Chinese herb therapy.
2.Effect of yisui shengxue granule on gene expression in children with beta-thalassemia.
Li-min CHAI ; Zhi-kui WU ; Xin-hua ZHANG ; Huiguo CAI
Chinese Journal of Integrated Traditional and Western Medicine 2005;25(7):591-594
OBJECTIVETo investigate the mechanism of yisui shengxue granule (YSG) in children with betathalassemia.
METHODSThe changes of hemoglobin (Hb), red blood cells (RBC), reticulocyte (Ret) and fetal hemoglobin (HbF) of 20 children with beta-thalassemia were measured before and after treatment. The effect of YSG on gene expression in myelo-karyocytes in 3 selected children treated effectively by YSG was determined by using DDRT-PCR.
RESULTSThe hematologic parameters were significantly enhanced and the ferritin gene expression declined after treatment respectively, as compared with those before treatment, the difference were significant (P < 0.01 or P < 0.05).
CONCLUSIONYSG could improve the clinical symptoms of children with beta-thalassemia. One of its therapeutic mechanisms may be its action in decreasing ferritin gene expression so as to effectively relieve the accumulation of iron in body.
Base Sequence ; Child ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Ferritins ; biosynthesis ; genetics ; Gene Expression Profiling ; Humans ; Male ; Molecular Sequence Data ; Phytotherapy ; RNA, Messenger ; biosynthesis ; genetics ; beta-Thalassemia ; drug therapy ; genetics
3.The inhibitory effect of chronic lead exposure on CA1 LTP and ERK2 activity in rat hippocampal area.
Jing YANG ; Li-guang SUN ; Zhi-hong ZHONG ; Kui CHAI ; Hong-xin WANG
Chinese Journal of Applied Physiology 2006;22(3):326-328
AIMTo investigate the effect of chronic lead exposure on rat hippocampal CA1 LTP and alpha-Ca2+ /calmodulin-dependent protein kinase II (alpha-CaM K II) activity in vivo.
METHODSA stimulus bipolar electrode was placed on the Schaffer/Commissural fibers, with extra cellular microelectrode technique to record the population spike (PS) in the CA1 pyramidal, and we observed the changes of PS amplitude before and after the high frequency stimulation (HFS) of lower, mid and higher level lead exposure groups and the control group, respectively. The hippocampal CA1 alpha-CaM K II activity was determined by Western blots by using phosphorylation antibody.
RESULTSThe average changes of PS after HFS of the control group, the lower, mid and higher level lead exposure groups were 162.5%, 105.2%, 86.8%, 83.0%, respectively (P < 0.01 vs. control). Defined control a-CaM K II activity as 100, that the lower, mid and higher level lead exposure groups were 62.0 +/- 3.7, 50.8 +/- 4.0, 43.3 +/- 4.1 (P < 0.01).
CONCLUSIONChronic lead exposure can inhibit CA1 LTP in vivo, and the decrease of alpha-CaMK II activity may play an important role in this mechanism.
Animals ; CA1 Region, Hippocampal ; metabolism ; Calcium-Calmodulin-Dependent Protein Kinase Type 2 ; metabolism ; Lead ; toxicity ; Long-Term Potentiation ; Rats ; Rats, Wistar ; Toxicity Tests, Chronic
4.Periodontal Wound Healing of the Experimental Subepithelial Connective Tissue Graft in Dogs.
Hyun Chul JUNG ; Seong Ho CHOI ; Kyoo Sung CHO ; Jung Kui CHAI ; Chong Kwan KIM
The Journal of the Korean Academy of Periodontology 1997;27(2):379-394
Several methods have been used for regeneration of tissue lost by periodontal disease. Subepithelial connective tissue graft technique, one of the technniques of mucogingival surgery, is used for the regeneration in esthetic problems such as recession, and denuded root coverage. This study is performed to evaluate the healing process and the regeneration and reattachment of periodontal tissue, including the reconstruction of junctional epithelium, and connective tissue. Alveolar defects in five adult dogs were treated with periodontal surgery and were attained by removing the marginal alveolar bone by 4x3mm from CEJ in the labial side of incisors, and root surfaces were planed. The experimental sites were divided into two groups as follows. 1. root planing alone(control group) 2. with connective tissue graft(Experimental Group) In the two groups flaps were positioned and sutured tightly, the healing processes were observed and were histologically compared with each other after 2days, 4days, 1week, 2weeks, 4weeks. The results were obtained as follows : 1. In the two groups blood clots were observed as early as 2 and 4 days, and were resorbed at 1 week. 2. In the two groups moderate inflammation was observed as early as 2 and 4 days, decreased at 1 and 2 weeks, and disappeared at 4 weeks. 3. Junctional Epithelium migration was more significant in the control group, and was restrained by graft materials in the experimental group. 4. Features of connective tissue fiber attachment partially showed the parallel pattern in the two groups from 2 weeks, and entirely from 4weeks. 5. Anastomosis, between graft and connective tissue, appeared from 4 days in the experimental group and the border between them was not discriminated at 4weeks.
Adult
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Animals
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Connective Tissue*
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Dogs*
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Epithelial Attachment
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Humans
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Incisor
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Inflammation
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Periodontal Diseases
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Regeneration
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Root Planing
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Tooth Cervix
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Transplants*
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Wound Healing*
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Wounds and Injuries*
5.A remote controlled multimode micro-stimulator for freely moving animals.
Wei-Guo SONG ; Jie CHAI ; Tai-Zhen HAN ; Kui YUAN
Acta Physiologica Sinica 2006;58(2):183-188
This paper presents a remote controlled multimode micro-stimulator based on the chip nRF24E1, which consists mainly of a micro-control unit (MCU) and a radio frequency (RF) transceiver. This micro-stimulator is very compact (18 mmx28 mm two layer printed circuit board) and light (5 g without battery), and can be carried on the back of a small animal to generate electrical stimuli according to the commands sent from a PC 10 meters away. The performance and effectiveness of the micro-stimulator were validated by in vitro experiments on the sciatic nerve (SN) of the frog, where action potentials (APs) as well as artifacts were observed when the SN was stimulated by the micro-stimulator. It was also shown by in vivo behavioral experiments on operant conditioned reflexes in rats which can be trained to obey auditory instruction cues by turning right or left to receive electrical stimulation ('virtual' reward) of the medial forebrain bundle (MFB) in a maze. The correct response for the rats to obey the instructions increased by three times and reached 93.5% in an average of 5 d. This micro-stimulator can not only be used for training small animals to become an 'animal robot', but also provide a new platform for behavioral and neurophysiological experiments.
Acoustic Stimulation
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Animals
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Behavior Control
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methods
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Conditioning, Operant
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physiology
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Electric Stimulation
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instrumentation
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Equipment Design
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Male
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Medial Forebrain Bundle
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physiology
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Movement
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Rats
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Rats, Sprague-Dawley
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Remote Sensing Technology
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Robotics
6.Hemodynamics and aortic tension induced by two septic shock models in rats.
Man-li XIA ; Min WANG ; Rong-kui CHAI ; Ce XU ; Hui-ping WANG ; Qiang XIA
Journal of Zhejiang University. Medical sciences 2010;39(6):572-576
OBJECTIVETo compare the ventricular-dynamic parameters and thoracic aorta tension induced by two septic shock models in rats.
METHODSSeptic shock models were induced by cecal ligation or puncture (CLP) and intraperitoneal injection of lipopolysaccharide (LPS) in rats. The carotid artery was cannulated and connected to a pressure transducer to determine mean arterial blood pressure (MABP). Ventricular dynamic parameters, including heart rate (HR), left ventricular developed pressure (LVDP) and maximal rise/fall velocity of ventricular pressure (± dP/dtmax) were determined. Isolated thoracic rings were mounted on an organ bath and the tension of the vessel was recorded.
RESULTThe mortality was 65.2% in CLP shock rats, but no death in LPS shock rats. The MABP and HR of CLP rats were decreased more prominently than those of LPS rats (P < 0.01). Contraction induced by high K(+) (60 mmol/L) or 10⁻⁶ mol/L phenylephrine (PE) in endothelium-intact and endothelium-denuded aortic rings was all attenuated, but in LPS rats it was more prominent (P < 0.01).
CONCLUSIONTwo rat septic shock models can decrease ventricular-dynamic parameters and vasoconstriction responsiveness of aorta. The ventricular-dynamic parameters decrease more prominently in CLP model, while vasoconstriction responsiveness of aorta changes more in LPS model.
Animals ; Cecum ; surgery ; Disease Models, Animal ; Hemodynamics ; Ligation ; adverse effects ; Lipopolysaccharides ; toxicity ; Male ; Random Allocation ; Rats ; Rats, Sprague-Dawley ; Shock, Septic ; etiology ; physiopathology ; Vasoconstriction ; Ventricular Pressure ; physiology
7.Antimicrobial and clinical effects of mouthrinses of acid water prepared by an electrolysis apparatus on chronic periodontitis.
Kyoo Sung CHO ; Mi Sook WON ; Hyun Chul JUNG ; Jung Hwak JUNG ; Seong Ho CHOI ; Jung Kui CHAI ; Chong Kwan KIM
The Journal of the Korean Academy of Periodontology 1997;27(4):739-749
The purpose of this study was to assess the antimicrobial and clinical effects of acid water mouthrinse prepared by an electrolysis apparatus on chronic periodontitis and to evaluate the lasting period of these effects. The change in the pattern of colonization of bacteria within the subgingival pockets was monitored by phase contrast microscopy, in 40 patients, over a period of 8 weeks. In addition, changes in the clinical parameters of the diseased sites were also monitored. Site of pocket > or = 5mm was selected in each patient randomly divided into two groups. As a test group, acid water mouthrinse was used twice a day in 20 patients. As control, no mouthrinse was used in 20 patients The results were as follows: 1. The suppression of motile bacteria was maintained for up to 3-4 weeks at test group. 2. Two groups did not differ significantly in proportion of bacteria in subgingival plaque over a period of 8 weeks. 3. Loss of attachment showed a significant difference in test group and in test group compared with control group (P<0.05), but there was no significant difference in control group. 4. No statistical difference was shown in two groups concerning the gingival index, plaque index, bleeding index. The results suggest that acid water mouthrinse is effective for reducing subgingival bacteria. It can be concluded that acid water may be useful as an mouthrinsing agent.
Bacteria
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Chronic Periodontitis*
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Colon
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Electrolysis*
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Hemorrhage
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Humans
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Microscopy, Phase-Contrast
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Periodontal Index
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Periodontitis
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Water*
8.Correlation between increased spinal cord signal intensity on T2-weighted MRI and clinical prognosis of compressive cervical myelopathy.
Kui-zhong ZHANG ; Hai-hong TU ; Zhi-li LIU ; Xiao-liang LOU ; Jian-sheng CHAI ; Tie ZHANG ; Rong-ping ZHOU
Journal of Southern Medical University 2009;29(10):2018-2020
OBJECTIVETo analyze the correlations between increased spinal cord signal intensity on magnetic resonance images (MRI) and the clinical prognosis of compressive cervical myelopathy.
METHODSSixty-six patients with cervical spondylotic myelopathy underwent surgeries through the anterior approach. In all the patients, the diagnoses were established on the basis of both neurological examination and MRI findings that showed spinal cord compression. The patients were divided into two groups according to preoperative MRI, namely isointense MRI T1/T2 signal group and iso/hyperintense MRI T1/T2 group. The JOA scores of the patients were evaluated before and at 6 and 12 months after the operation.
RESULTSThe patients were followed up for 12 to 38 months after the operation (mean 21 months), and no statistically significant difference were found in the pre- and postoperative JOA scores between the two groups (P>0.05).
CONCLUSIONThe peoperative hyperintense signals on T2 weighted MRI does not correlate to the prognosis of patients with compressive cervical myelopathy, who may also have favorable clinical outcomes after the operation.
Adult ; Cervical Vertebrae ; pathology ; surgery ; Female ; Humans ; Magnetic Resonance Imaging ; Male ; Middle Aged ; Prognosis ; Spinal Cord Compression ; diagnosis ; etiology ; surgery ; Spinal Osteophytosis ; complications ; diagnosis ; surgery
9.Effect on expression of mice alpha-hemoglobin stabilizing protein in different developmental stages treated with Yisui Shengxue granules.
Yong-Mei LIU ; Zhi-Kui WU ; Li-Min CHAI ; Xin-Hua ZHANG ; Min LI ; Yu-Ying CHEN ; Xin-Xia LV ; Xiao-Yun ZHU
China Journal of Chinese Materia Medica 2007;32(7):609-612
OBJECTIVETo discuss the effect of Yisui Shengxue granules on expression of alpha-hemoglobin stabilizing protein (AHSP) mRNA in different developmental stages mice.
METHODThe total RNAs were extracted from the bone marrow karyocyte of normal adult mice and the karyocyte of fetus liver and fetus spleen in pregnanted mice (pregnanted 21 days) and fetal mice (pregnanted 14 days). The expression level of AHSP mRNA in different developmental stages mice interfered with Yisui Shengxue granules was measured by real-time PCR.
RESULTThe intervention of Yisui Shengxue granules could significantly up-regulated the expression levels of AHSP mRNA in normal adult mice.
CONCLUSIONThe result revealed that one of possible molecular mechanism of the effects caused by Yisui Shengxue granules is that it can promote the AHSP gene expression, reduce the free a-globin deposit, then prevent the poison to erythrocyte and decrease the haemolysis.
Animals ; Blood Proteins ; genetics ; Bone Marrow Cells ; cytology ; drug effects ; metabolism ; Drug Combinations ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Erythrocytes ; cytology ; drug effects ; metabolism ; Female ; Gene Expression Regulation, Developmental ; drug effects ; Liver ; cytology ; embryology ; metabolism ; Male ; Mice ; Molecular Chaperones ; genetics ; Plants, Medicinal ; chemistry ; Pregnancy ; RNA, Messenger ; biosynthesis ; genetics ; Random Allocation ; Spleen ; cytology ; embryology ; metabolism ; Up-Regulation ; drug effects ; genetics
10.A Prognostic Nomogram Based on Response to Bortezomib and BTK Expression for Treatment-Experienced Multiple Myeloma Patients.
Hui-Ling CHEN ; Cheng-Cheng MA ; Ye CHAI ; Peng-Yun ZENG ; Chong-Yang WU ; Ling-Ling YUE ; Ti-Yun HAN ; De-Kui ZHANG
Journal of Experimental Hematology 2022;30(4):1139-1143
OBJECTIVE:
To establish a prognostic nomogram based on response to bortezomib and BTK expression for treatment-experienced multiple myeloma patients.
METHODS:
The Oncomine database was utilized to determine BTK expression, sex, age, albumin, Mayo index, response to bortezomib treatment, follow-up time and survival status in multiple myeloma(MM) patients. Cut-off point for BTK expression was calculated using R software. Univariate and multivariate analyses by Cox proportional hazards regression were then performed. Significant prognostic factors were combined to build a nomogram. The discrimination ability and predictive accuracy of the nomogram were evaluated using the index of concordance (C-index) and calibration curves.
RESULTS:
Multivariate analysis showed that response to bortezomib, BTK expression and sex were independent risk factors for prognosis. The C-index value of the nomogram made according to the independent risk factors was 0.729 (95%CI, 0.642-0.8164). The calibration curves showed good consistency between predicted and actual survivals for 1-year and 2-year overall survival.
CONCLUSION
The proposed nomogram is accurate in predicting the prognosis of patients with MM.
Bortezomib/therapeutic use*
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Humans
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Multiple Myeloma/drug therapy*
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Nomograms
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Prognosis
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Proportional Hazards Models