2.Effects of bFGF and alpha-MSH on adhesion and migration of human melanocytes in vitro.
Xian-qi ZHANG ; Jie FENG ; Kuan-hou MOU ; Hui-qun MA ; Xin-wu NIU ; Chao LIU
Journal of Zhejiang University. Medical sciences 2006;35(2):161-164
OBJECTIVETo observe the effect of basic fibroblast growth factor (bFGF) and alpha-melanocyte stimulating hormone (alpha-MSH) on adhesion and migration of melanocytes in vitro.
METHODSHuman melanocytes were obtained from normal human foreskins. Culture dishes covered with fibronectin were used to perform melanocytes adhesion assay, and cell motility was assessed using the Transwell micropore filter method.
RESULTbFGF and alpha-MSH increased melanocytes adhesion on culture dishes covered with fibronectin. bFGF stimulated melanocytes migration through micropore filter while alpha-MSH had no significant effects.
CONCLUSIONbFGF and alpha-MSH could promote the adhesion and migration of melanocytes, which suggests that two agents may play a role in the repigmentation of vitiligo.
Cell Adhesion ; drug effects ; Cell Movement ; drug effects ; Cells, Cultured ; Fibroblast Growth Factor 2 ; pharmacology ; Humans ; Melanocytes ; cytology ; alpha-MSH ; pharmacology
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