1.Moxifloxacin-warfarin Interaction
Kuan Leong Yew ; Wan Chieng Lee
The Medical Journal of Malaysia 2012;67(4):420-421
Warfarin has its origin as a rat poison and has become one
of the widely used anticoagulant drugs to prevent thrombosis and embolism. Many medications, foods and vegetables can interact with warfarin and affect its blood thinning effect in the body. The antibiotic, moxifloxacin official product monograph indicated no interaction with concomitant warfarin treatment as this fourth generation synthetic fluoroquinolone antibiotic does not share the same cytochrome P450 system metabolism pathway with warfarin.
However, we report a case of increased anticoagulant activity in a patient treated with moxifloxacin and warfarin causing prolonged hospital stay.
2.Primary Study on Noninvasive Detection of Vascular Function Based on Finger Temperature Change.
Qing DONG ; Xia LI ; Yungao WAN ; Gaoquan LU ; Xinxin WANG ; Kuan ZHANG
Journal of Biomedical Engineering 2016;33(1):144-148
By studying the relationship between fingertip temperature changes and arterial function during vascular reactivity test, we established a new non-invasive method for detecting vascular function, in order to provide an assistance for early diagnosis and prevention of cardiovascular diseases. We customized three modules respectively for blood occlusion, measurement of finger temperature and blood oxygen acquisition, and then we established the hardware of data acquisition system. And the software was programmed with Labview. Healthy subjects [group A, n = 24, (44.6 ± 9.0) years] and subjects with cardiovascular diseases [group B, n = 33, (57.2 ± 9.9) years)] were chosen for the study. Subject's finger temperature, blood oxygen and occlusion pressure of block side during and after unilateral arm brachial artery occlusion were recorded, as well as some other regular physiological indexes. By time-domain analysis, we extracted 12 parameters from fingertip temperature signal, including the initial temperature (Ti), temperature rebound (TR), the time of the temperature recovering to initial status (RIt) and other parameters from the finger temperature signal. We in the experiment also measured other regular physiological body mass index (BMI), systolic blood pressure (SBP), diastiolic blood pressure (DBP) and so on. Results showed that 8 parameters difference between the two group of data were significant. based on the statistical results. A discriminant function of vascular function status was established afterwards. We found in the study that the changes of finger temperature during unilateral arms brachial artery occlusion and open were closely related to vascular function. We hope that the method presented in this article could lay a foundation of early detection of vascular function.
Adult
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Aged
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Arteries
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physiopathology
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Blood Pressure
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Cardiovascular Diseases
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diagnosis
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Fingers
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Humans
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Middle Aged
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Skin Temperature
3.Dietary Supplements: Usage And Opinions Among Health Sciences Students
Cheong Lieng Teng ; Kim Kuan Tey ; Pek Hong Lim ; Shian Feng Cheng ; Mohd Safwan Nordin ; Ching Mun Ng ; Wan Nurzahiah Wan Zakaria ; Khung Ying Wong
International e-Journal of Science, Medicine and Education 2008;2(2):30-32
This is a questionnaire survey of dietary
supplement usage among students in the International
Medical University. Just over two-fifths of these
students reported using dietary supplements daily. This
high usage of dietary supplements is in contrast their
expressed ambivalence about these products.
4.Effects of electro-acupuncture on expression of triggering receptor expressed on myeloid cells 1 in ankle joint synovial tissue of acute gouty arthritis rats.
Chao-Nan ZHANG ; Xue-Kuan HUANG ; Yan LUO ; Juan JIANG ; Lei WAN ; Ling WANG
Journal of Southern Medical University 2015;35(1):133-136
OBJECTIVETo investigate the effects of electro-acupuncture (EA) on the expression of triggering receptor expressed on myeloid cell (TREM)l in ankle joint synovial tissue of acute gouty arthritis (AGA) rats.
METHODSForty male SD rats were randomly divided into 4 groups: normal, AGA, medication and EA group, 10 rats in each group. AGA model was established by induced monosodium urate (MSU) method, except the normal group. Tow days before AGA model was established, normal and AGA groups were lavaged with normal saline (20 ml/kg), medication group was lavaged with colchicine solution (20 ml/kg), EA(1.5-2 Hz, D.-D.wave, 9v; 1-3 rnA) was applied to "Sanyinjiao" (SP6), "jiexi" (ST41) and "Kunlun" (BL60) for 20 min, once daily;continuously for 9 days. Then observed the changes in dysfunction, and the content of TNF-α and IL-lβ detected by ELISA, the expression of TREM-l detected by immunohistochemistry and western blot.
RESULTSCompared to the normal group, the AGA group of the dysfunction index increased significantly (P<0.01), the content of TNF-α and IL-lβ increased significantly (P<0.05), the expression of TREM-l in synovial tissue increased significantly (P<0.05); the medication and EA groups compared to the AGA group, the dysfunction index decreased significantly (P<0.01), the content of TNF-α and IL-lβ decreased significantly (P<0.05), the expression of TREM-l in synovial tissue decreased significantly (P<0.05); there were not statistically significant between the medication and EA group (P>0.05).
CONCLUSIONEA treating AGA may be through down-regulating the expression of TREM -1 in synovial tissue.
Animals ; Ankle Joint ; metabolism ; pathology ; Arthritis, Gouty ; metabolism ; therapy ; Electroacupuncture ; Interleukin-1beta ; metabolism ; Male ; Rats ; Rats, Sprague-Dawley ; Receptors, Immunologic ; metabolism ; Synovial Membrane ; metabolism ; Triggering Receptor Expressed on Myeloid Cells-1 ; Tumor Necrosis Factor-alpha ; metabolism
5.The Antitumor Activity of G-CSF-Mobilized Peripheral Blood Mononuclear Cells Activated by IL-2 Alone or in Combination with IL-12
Wan-Jun SUN ; Zi-Kuan GUO ; Hui-Sheng AI
Journal of Experimental Hematology 2001;9(4):333-337
This study was undertaken to investigate the antitumor activity of rhG-CSF mobilized blood mononuclear cells (G-PBMC) from normal donor activated by rhIL-2 alone or in combination with rhIL-12 in vitro. LDH release assay was used to determine the cytotoxicity activity of G-PBMC against K562 (NK-sensitive) and Raji cells (NK-resistant). The phenotype of the activated G-PBMC was assayed by flow cytometry. Results suggested that the cytotoxicity of the fresh G-PBMC was low before IL-2/IL-12 stimulation. G-PBMC developed marked cytotoxicity after 24 hours of IL-2 activation, with more significant increase for further 48 hours induction. When G-PBMCs were exposed to combination of IL-2 and IL-12, the cytotoxicity was significantly enhanced. The proportions of CD3(+) T cells and CD8(+) T cells were increased when G-PBMC incubated with IL-2 for 72 hours. However, CD56(+) cells were significantly elevated when G-PBMC exposed to IL-2 and IL-12 for 7 days. It is concluded that G-PBMC activated by IL-2 had evident antitumor activity, which was further increased when IL-2 in combination with IL-12. These results demonstrate that IL-2 and IL-12 treatment in vitro might promote the graft-versus-leukemia (GVL) response of G-PBMC.
6.Application of PCR-RFLP in detection of adefovir dipivoxil resistance-associated mutations in hepatitis B virus
Ri-Cheng MAO ; Ji-Ming ZHANG ; You-Kuan YIN ; Yan-Li QIN ; Wan-Qin ZHANG ; Xiang-Hui WU ; Xin-Hua WENG ;
Chinese Journal of Infection and Chemotherapy 2007;0(05):-
Objective To establish a convenient,accurate and practical method for detection of adefovir dipivoxil resistance-as- sociated mutation in hepatitis B virus:rtA181V/T/S and rtN236T mutations.Methods According to HBV complete sequences in GenBank,two pairs of primers were designed to amplify the region of HBV reverse transcriptase in order to introduce a BglI restriction site upon PCR product of wild type (wt) and a BseDI restriction site upon PCR product of rt236 mutant type.After amplification,the PCR products were digested with BglI and BseDI separately.We used this method to detect wild,rt181 mu- tant,rt236 mutant plasmids and 3 chronic hepatitis B patients' serum with obvious ADV resistance-associated mutations.We also tested the sensitivity of this method by mixing the wild and mutant plasmids in different proportions.Results The method could detect rt181 and rt236 mutations simultaneously.The result of RFLP analysis was in accordance with that of DNA se- quencing and cloning analysis.This method could detect the mutants even when they comprised only 10% of the total virus population.Conclusions The PCR-RFLP method with high sensitivity can detect rt181 and rt236 mutations simultaneously.It can be used for early detection of ADV resistance-associated mutation in hepatitis B virus.
7. Analysis of silica dust detection results in workplace air of somewhere in enterprise
Kuan WAN ; Yehua TANG ; Weiyi ZHANG ; Haiying PAN ; Yaozhong QIAN ; Lianhong ZHANG ; Yufeng SHEN ; Cuiping FANG
Chinese Journal of Industrial Hygiene and Occupational Diseases 2019;37(11):823-826
Objective:
To understand the occupational hazard and distribution of silica dust (free SiO2≥10%) in the workplace environment of the enterprises in Fengxian District, and to provide scientific basis for improving the working environment and protecting the physical and mental health of the workers.
Methods:
Individual sampling monitoring and on-site labor hygiene investigation were conducted on 421 workers involved in 87 silicon dust enterprises in the jurisdiction from 2014 to 2018, and measured concentration-time weighted average (
8.Chylous leakage after retroperitoneoscopic upper-pole heminephrectomy for duplex kidney.
Yueming WAN ; Zhengyan TANG ; Zhiyong CHEN ; Wen TIAN ; Lun XIAO ; Kuan LUO ; Dongjie LI
Journal of Central South University(Medical Sciences) 2012;37(4):405-407
OBJECTIVE:
To discuss management of chylous leakage after retroperitoneoscopic upper-pole heminephrectomy for duplex kidney.
METHODS:
Between November 2004 and Februar y 2011, 39 patients underwent retroperitoneoscopic upper-pole heminephrectomy for duplex kidney, of these 5 patients had chylous leakage. The ages of the patients ranged from 32 to 60 years (mean 42). All the patients were treated conservatively, and the therapeutic effects were observed.
RESULTS:
Delayed chylous leakage in 5 patients occurred 5-31 days after surgery, and leakage occurred in 4 of the same 5 patients during the first 2 post-operative years. Chylous leakage after retroperitoneoscopic upper-pole heminephrectomy for duplex kidney preferentially occurred at the left side of duplex kidney. All the patients healed under conservative treatment.
CONCLUSION
Chylous leakage typically occurs after left retroperitoneoscopic upper-pole heminephrectomy for duplex kidney, and can be prevented by improving surgical technique; it can be completely relieved by conservative management with satisfactory results.
Adult
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Chylous Ascites
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etiology
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prevention & control
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therapy
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Female
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Humans
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Kidney
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abnormalities
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surgery
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Laparoscopy
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adverse effects
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methods
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Male
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Middle Aged
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Nephrectomy
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adverse effects
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methods
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Retroperitoneal Space
9.A Case of Acute Staphylococcal aureus Esophagitis in Immunocompromised Patients.
Hwan Yang PARK ; Chun Kuan KIM ; Young Bae PARK ; Jae Wan PARK ; Sung Hun KIM ; Jung Won KANG ; Kyoung Hyoub MOON ; Soo Hyun YANG ; Jong Hon BYUN
Korean Journal of Infectious Diseases 2001;33(5):360-363
Bacterial esophagitis is an uncommon disease and has not been well characterized. Bacterial infection of the esophagus is usually presented as a superimposed infection upon a preexisting viral or fungal esophagitis and most patients are immunocompromised hosts. A 67-year-old man was admitted for retrosternal pain and hematemesis, who had a past history of long-standing diabetes mellitus and end stage renal disease, also had a history of steroid medication. Extensive esophageal ulcerations of the mucosa were visualized by endoscopy. Staphylococcus aureus grew in blood culture. After the 2 weeks of antibiotics treatment, he was successfully recovered without any sequelae. Due to its rarity, this case is herein reported with a review of the corresponding literature.
Aged
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Anti-Bacterial Agents
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Bacterial Infections
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Diabetes Mellitus
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Endoscopy
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Esophagitis*
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Esophagus
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Hematemesis
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Humans
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Immunocompromised Host*
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Kidney Failure, Chronic
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Mucous Membrane
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Staphylococcus aureus
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Ulcer
10.Predictive value of fluorescence in situ hybridization in patients with bladder cancer.
Zheng-fei SHAN ; Shao-bin ZHENG ; Peng WU ; Wan-long TAN ; Yi ZUO ; Hai-kuan ZHOU ; Huan QI ; Peng ZHANG ; Hong-mei PENG
Journal of Southern Medical University 2010;30(7):1597-1603
OBJECTIVETo assess the value of fluorescence in situ hybridization (FISH) in the diagnosis of bladder cancer.
METHODSUrine samples from 100 patients suspected of having bladder cancer were collected before cystoscopy for immediate urine cytology and FISH analysis. The criteria for FISH abnormality were determined by evaluating the urine specimens from 20 subjects without urogenital neoplasm.
RESULTSThe overall sensitivity of cytology and FISH was 43.2% and 82.4%, and their specificity was 92.3% and 88.5%, with diagnostic concordance rate of 56.0% and 84.0%, respectively. The differences between FISH and cytology showed statistical significance in the sensitivity, diagnostic concordance rate, non-muscle-invasive cancer and primary cancer.
CONCLUSIONThe sensitivity and efficiency of FISH in the detection of bladder cancer are superior to those of cytology, especially for prophase cancer.
Adolescent ; Adult ; Aged ; Aged, 80 and over ; Carcinoma, Transitional Cell ; diagnosis ; Cytodiagnosis ; Female ; Humans ; In Situ Hybridization, Fluorescence ; Male ; Middle Aged ; Sensitivity and Specificity ; Urinary Bladder Neoplasms ; diagnosis ; Urine ; cytology ; Young Adult