Objective To investigate Insertion/Deletion (InDel) polymorphism on the X chromosome and to screen 18 InDel loci for the Chinese Han population as a forensic DNA typing system auxiliary. Meth-ods Eighteen X-InDel markers were selected using the Human Genome Browser and dbSNP database. Multiplex PCR primer pairs of selected X-InDel markers were designed using Primer 3 software and di-vided into 3 groups according to the amplified fragment length, labeled by FAM, HEX and TAMRA fluorescence dye, respectively. The population genetics research and comparative analysis of Chinese Han nationality and 4 main minorities, the Hui, Wei, Mongol, and Tibetan nationalities, were investigated with the system. Results A new multiplex genotyping system, named InDel X-18PLEX, was successfully developed and validated, consisted of 18 X-InDel markers on the X chromosome and 1 Amelogenin gen-der marker. No deviation from Hardy-Weinberg equilibrium expectations was detected in the distribution of genotypes in the 5 investigated ethnic groups. However, there was significant difference between their distributions. From the investigation of Han nationality, high female (0.999 999 4) and male (0.999 88) overall discrimination power values were obtained, as well as high overall mean exclusion chance values in trios (0.999 992) and in duos (0.99). Conclusion InDel X-18PLEX meets the requirements as a forensic DNA complementary kit, providing effective supplementary analytical tools for difficult cases.

No abstract available.

Objective To determine the effective accumulated temperature of Oncomelania hu-pensis during the period of development of generations in the natural environment. Methods The snails, Oncumelania hupensis, were raised in natural breeding site, the developing duration and accumulated temperature for .snails' development from eggs to adult that began to lay eggs were estimated based on the formula T=?(Ti-C). Results The average developing duration was (334.22?7. 52) days, average accumulated temperature was (5 821.38?70.05) day degree, and average effective accumulated temperature was (3 846.28?32. 59) day degree. Conclusion The method to estimate the effective accumulated temperature is quite accuracy, which provides theoretical parameters in developing the prediction model on transmission of schistosomiasis japonica affected by weather warming.

Objective To explore the aestivation and lethal hyperthermy temperature of Oncomelania hupensis in summer,which is one of important ecology indexes,in order to understand the potential impact of global warming on the distribution of Oncomelania snail in mainland China. Methods Oncomelania hupensis hupensis snails were collected from the marshland region in Jiangsu Province and the aestivation and lethal hyperthermy temperature were examined by increasing temperature gradually in laboratory. Results The lethal hyperthermy temperatures of 50% Oncomelamia snails in dry and wet environment was 40.01℃ ( 95% of confidence interval from 39.76-40.27℃) and 42.13℃( 95% of confidence interval from 41.59-42.68℃), respectively. The logistic regression equations between temperature and mortality were d=101/(1+e 61.402269-1.535058X) (F=69.997,P

OBJECTIVE: To investigate Insertion/Deletion (InDel) polymorphism on the X chromosome and to screen 18 InDel loci for the Chinese Han population as a forensic DNA typing system auxiliary. METHODS: Eighteen X-InDel markers were selected using the Human Genome Browser and dbSNP database. Multiplex PCR primer pairs of selected X-InDel markers were designed using Primer 3 software and divided into 3 groups according to the amplified fragment length, labeled by FAM, HEX and TAMRA fluorescence dye, respectively. The population genetics research and comparative analysis of Chinese Han nationality and 4 main minorities, the Hui, Wei, Mongol, and Tibetan nationalities, were investigated with the system. RESULTS: A new multiplex genotyping system, named InDel X-18PLEX, was successfully developed and validated, consisted of 18 X-InDel markers on the X chromosome and 1 Amelogenin gender marker. No deviation from Hardy-Weinberg equilibrium expectations was detected in the distribution of genotypes in the 5 investigated ethnic groups. However, there was significant difference between their distributions. From the investigation of Han nationality, high female (0.9999994) and male (0.999 88) overall discrimination power values were obtained, as well as high overall mean exclusion chance values in trios (0.999 992) and in duos (0.99). CONCLUSION InDel X-18PLEX meets the requirements as a forensic DNA complementary kit, providing effective supplementary analytical tools for difficult cases.
Amelogenin/genetics* ; Asian People ; DNA Fingerprinting ; DNA Primers ; Ethnicity ; Female ; Gene Frequency ; Genetics, Population ; Genome, Human ; Genotype ; Humans ; INDEL Mutation ; Male ; Multiplex Polymerase Chain Reaction/methods* ; Polymorphism, Genetic

ObjectiveTo explore the clinical efficacy and safety of autologous peripheral blood stem cell transplantation(APBSCT) in the treatment of pemphigus.MethodsTotally,3 patients with pemphigus vulgaris who responsed poorly to 6-month treatment with glucocorticoids or immunosuppressants or experienced aggrevation of disease and developed treatment-related complications,received APBSCT and were followed up for more than 5 years.There were 1 male and 2 females with an average age of 27.3(21-39) years.The mobilization program included cyclophosphamide (CTX) 4 g/m2,recombinant human granulocyte colonystimulating factors(G-CSF) and Rituximab 375 mg/m2,and the preconditioning regimen included intravenous CTX (50 mg/kg per day on days -6,-5,-4,-3),antithymocyte globulin at 2.5 mg/kg per day(on days -3,-2,-1 and 0) and Rituximab (600 mg/d on days 0 and 7).ResultsAll the 3.patients were successfully engrafted.The mean time for peripheral reconstruction:white blood cells 13.3 days (from day 11 to 16),platelet 16.3 days (from day 16 to 17).Monitoring of immunity indices and related antibodies showed no abnormality and the immune system was well reconstructed.No serious complications occurred during the follow up,and the patients' quality of life was obviously improved.ConclusionAPBSCT may be an effective and safe option for the treatment of pemphigus.

The authors retrospecitviely analyzed the MRI findings of the 8 head-injured patiens who had showed persistent unconsciousness and severe neurologic deficite after trauma. T2-weighted MR images disclosed high signal intensity lesions of various size and shape at lobar white matter and corpus callosum, subcortical gray matter, brain stem, and cerebral cortex, and assisted greatly in understanding the neurologic deficits of the corresponding patients. The lesions were mostly multiple and involved multiple structures. CT's revealed in those patients only small hemorrhages, suspicious low densities, such nonspecific findings as slit ventricles and diffuse brain atrophy, or no abnormality at all. Post-traumatic parenchymatous lesions detected by MR images were either missed or quite underestimated on CT's. MRI was very supeior to CT in the detection and anatomic localization of nonhemorrhagic intraaxial cerebral injuries. especially in the cases of small corical contusions, brainstem and white matter injuries. For the evaluation of the acute posttraumatic patients, CT ios indicated in order to fastly detect intracranial hemagomas which may need emergent surgical evacuation. If CT findings does not explain the neurologic degicits of the patients, especially in the late stage. MRI is indicated. MRI is expected to play a major role in the diagnosis of shear injuries involoving white matter and brainstem where CT has been of little contribution or misleading.
Atrophy ; Brain ; Brain Stem ; Cerebral Cortex ; Contusions ; Corpus Callosum ; Diagnosis ; Hemorrhage ; Humans ; Magnetic Resonance Imaging* ; Neurologic Manifestations ; Unconsciousness

We report a rare case of cervical intradural extramedullary neurilemmoma associated with extradural cavernous angioma in adolescent male patient. This 19-year-old man complained of increasing weakness of left extremities with pain and numbness, starting upwards from the region of left leg since 2 months before, which has aggravated gradually and developed to right side. Neither the patient nor any family member displayed the stigmata of spinal tumor. Neurological examination showed spastic qudriparesis, more severe on left side, disclosed absent vibratory sense and diminution of pain and touch below level of cervico-thoracic junction. Myelogram demonstrated a large filling defect at the C4-6 vertebral level, which was interpreted as an intradural extramedullary or extradural mass. Laminectomy was performed 4 days after admission. At surgery, reddish, diffuse and well demarcated friable mass was found extradural space at the level of C4-6. But post-operative course was not improved for 2 weeks, so we are performed second operation after identified another intradural extramedullary mass by spinal CT. A total removal of tumor mass was accomplished at second procedure. Hostological examination confirmed the diagnosis of cavernous angioma at the extradural mass, neurilemmoma at the intradural extramedullary mass. The post-operative course was satisfactory.
Adolescent ; Christianity ; Diagnosis ; Extremities ; Hemangioma, Cavernous* ; Humans ; Hypesthesia ; Laminectomy ; Leg ; Male ; Muscle Spasticity ; Myelography ; Neurilemmoma* ; Neurologic Examination ; Young Adult

Objective To evaluate the implementation of the Control Program of Key Parasitic Diseases in Jinhu County from 2006 to 2015,so as to provide the evidence for the future work of prevention and control. Methods The historical data about key parasitoses were collected,and the organization management,financial support,capacity building,control effects, and so on were evaluated with the descriptive method.Results From 2006 to 2015,totally 19 technique educations were con-ducted,844.2 thousands RMB was invested,and 1 725 person-times of technicians were trained.Totally 197 600 person-times of drug administration were performed and 11 762 person-times of residents were tested for parasites,with the infection rates of soil-transmitted nematodes fluctuating from 0.16% to 2.18%. The infection rates of Clonorchis sinensis were from 0.00% to 0.67%,and the infection rates of its intermediate hosts were from 3.81% to 9.48%.No imago and larva of Angiostrongylus canto-nensis were found in the longitudinal surveillance.In 2013,the awareness rate of health related knowledge was up to 95.62%, and the correct rate of health behaviors was up to 96.46%.Totally 3 764 villages were renovated,and the beneficial rate of tap-water was up to 98.90%. The popularity rate of household toilets was up to 95.31%,and the popularity rate of harmlessness health toilets was up to 93.45%.Conclusion The endemic situation of key parasitoses is in a low state in Jinhu County,howev-er,the transmitted risk still exists,and therefore,the surveillance work need to be enhanced.

OBJECTIVETo study the polarization and immune regulation of TH cells in patients with bronchial asthma.

METHODSThirty-eight hospitalized children with bronchial asthma (ranging from 4-12 years old) and 29 age-matched healthy children (Control group) were enrolled in this study. Serum IL-2 and IFN-gamma levels were detected using ELISA. The percentage of TH1 and TH2 cells was detected by intracellular staining.

RESULTSThe serum levels of IL-2 (15.94 +/- 3.07 microg/L) and IFN-gamma (487.2 +/- 43.6 pg/mL ) in asthmatic patients were significantly lower than those in the Control group (24.73 +/- 4.37 microg/L and 654.07 +/- 14.64 pg/mL respectively; P < 0.01). The percentage of TH1 in asthmatic patients decreased significantly compared with that in the Control group [(11.24 +/- 2.43)% vs (16.67 +/- 2.73)%; P < 0.01]; in contrast, the percentage of TH2 increased compared with that in the Control group [(19.85 +/- 4.46)% vs (16.08 +/- 6.17)%; P < 0.05].

CONCLUSIONSThe serum levels of IL-2 and IFN-gamma, and the number of TH1 cells decreased in asthmatic patients. The decreased number of TH1 cells and the ratio of TH1/TH2 suggest an abnormal polarization of TH1 and TH2 cells. The changes may be associated with the inhibition of cellular immune function in asthmatic patients.

Asthma ; immunology ; Cell Polarity ; Child ; Child, Preschool ; Female ; Humans ; Interferon-gamma ; blood ; Interleukin-2 ; blood ; Male ; Th1 Cells ; immunology ; Th2 Cells ; immunology