After subcutaneous injection of proper doses of phenylhydrazine to pregnant, youngand adult mice to induce experimental hemolysis, a systematic observation was made onthe developmental and differentiation processes of macrophages in the liver of 10--18 daymouse embryos, of the 1st, 4th, 14th, 20th day young mice and of adult mice. The re-sults were compared with that of the normal controls. Besides liver, the changes ofmacrophages in the spleen and bone marrow were also observed. In the 10th day embryos, in which the primordium of liver began to form, themesenchymal cells in the septum transversium (primitive diaphragm) were seen to dif-ferentiate into primitive macrophages. The later the developmental period of the mouseembryos, the greater is the number of mature macrophages. When adult stage wasreached, the macrophages became Kupffer cells. In the 10--14 day mouse embryos, thephagocytic inclusions in the macrophages appeared as non-homogenous droplets of unequalsize, whereas those found later in the embryonic life and after birth became homogenousgranules. In the early stage of the development of liver, these macrophages, as demons-trated by means of Perls' acid ferrocyanide reaction, contained only scattered and smallamount of iron granules. These iron granules gradually increase in number until latein the embryonic life, during which time they were seen to mass into groups. After birththey gradually reduced in number and disappeared entirely in mice aging 14 days on-ward. These changes indicated that the macrophages in the liver in ontogenesis hadgradually undergone morphological as well as functional differentiation. In the early stage of embryonic liver, the macrophages were found in the hepaticcords and on the walls of the sinusoids. At about the fifteenth day of the embryonic life,following the rearrangement of the hepatic cords, the macrophages originally locatedtherein have now become a part of the newly formed sinusoid walls. Thus the authorsbelieve that during embryonic stage, the macrophages on the sinusoid walls are not en-tirely derived from the endothelium of blood vessels. During the late stage of embryonic development, macrophages containing phagocyticinclusions were also found in the spleen and bone marrow. Only those macrophagesseen in the spleen showed positive Perls' reaction. After birth these macrophages andtheir iron granules in the spleen gradually increased in number. In adult life the spleenbecame the main site of iron reserve.

Objective To summarize our experience on the diagnosis and treatment of gastric smooth muscle tumor.MethodThe clinical data of 34 cases with gastric smooth muscle tumor were analyzed retrospectively.ResultLeiomyosarcomas were ≥5 cm in diameter in 68% of all cases, and ≥10 cm in 50%. Most leiomyosarcomas were located in the fundus and corpus of the stomach (94%). Some patients of gastric leiomyosarcoma have the triad of epigastric pain, mass, and bleeding. Gastroscopy examination often reveals a mass lying submucously. Total or partial gastrectomy was performed for all patients based on tumor size and locus. The 3, 5, 10-year′s survival rate were 87%, 52% and 26% respectively. Conclusion Most gastric leiomyosarcomas are characteristic of local invasiveness, postoperative survival is often favourable.

Objective To probe the application value of radionuclide bone imaging in pre-treatment selection of therapeutic plan and regular post-treatment follow-up of patients with primary pulmonary cancer.Methods 810 patients with primary pulmonary cancer had radionuclide bone imaging respectively before the treatment. The follow-up radionuclide bone imaging was performed in 492 cases that were treated by surgery in 2 years after the operation. For 318 cases that were treated by non-surgery way,only 142 cases that had no skeletal metastases had follow-up radionuclide bone imaging in 2 years after the treatment. Results 179 cases (22.1%) had skeletal metastases in 810 cases. Multiple skeletal lesions were found in 157 cases and single skeletal lesion found in 22 cases. The majority sites of skeletal metastases were ribs, vertebrae column and pelvis. 57 cases that were treated by surgery had new skeletal lesions in 2 years after the operation. For non-surgery patients,79 cases had new skeletal lesions in 2 years after the treatment. Conclusion Radionuclide bone imaging is useful in the selection of therapeutic plan and staging of primary pulmonary cancer before the treatment.Regular bone imaging after the operation is helpful to detect early bone metastasis and choose the therapeutic plan, and should be regarded as a foremost method to detect the skeletal lesion. Radionuclide bone imaging after the non-surgery treatment maybe help us to conclude the prognosis of the patients.

Objective: The aim of this study was to evaluate whether there is an association between Parkinson’s disease (PD) and colorectal cancer in Taiwan. Methods: This was a case-control study using claim data of the Taiwan National Health Insurance Program. There were 64,619 subjects aged 20-84 with newly diagnosed colorectal cancer as cases and 64,619 randomly selected subjects without colorectal cancer as controls from 2005 to 2011. Both cases and controls were matched by sex, age, comorbidities, and index year of diagnosing colorectal cancer. Subjects who were diagnosed with PD within 5 years of diagnosing colorectal cancer were excluded. The multivariable logistic regression model was used to estimate the adjusted odds ratio (OR) and 95% confidence interval (95% CI) for risk of colorectal cancer associated with PD. Results: After adjusting for confounding factors, the multivariable logistic regression analysis revealed that the adjusted OR of colorectal cancer was 0.69 for subjects with PD more than 5 years before index date (95% CI 0.59, 0.81), as compared with subjects without PD. Conclusion: PD is associated with reduced odds of colorectal cancer. Further research is needed to elucidate the mechanisms underlying our findings.

Adrenal Glands ; Female ; Hematopoiesis, Extramedullary ; Humans ; Spherocytosis, Hereditary ; complications ; Young Adult

Objective To investigate Insertion/Deletion (InDel) polymorphism on the X chromosome and to screen 18 InDel loci for the Chinese Han population as a forensic DNA typing system auxiliary. Meth-ods Eighteen X-InDel markers were selected using the Human Genome Browser and dbSNP database. Multiplex PCR primer pairs of selected X-InDel markers were designed using Primer 3 software and di-vided into 3 groups according to the amplified fragment length, labeled by FAM, HEX and TAMRA fluorescence dye, respectively. The population genetics research and comparative analysis of Chinese Han nationality and 4 main minorities, the Hui, Wei, Mongol, and Tibetan nationalities, were investigated with the system. Results A new multiplex genotyping system, named InDel X-18PLEX, was successfully developed and validated, consisted of 18 X-InDel markers on the X chromosome and 1 Amelogenin gen-der marker. No deviation from Hardy-Weinberg equilibrium expectations was detected in the distribution of genotypes in the 5 investigated ethnic groups. However, there was significant difference between their distributions. From the investigation of Han nationality, high female (0.999 999 4) and male (0.999 88) overall discrimination power values were obtained, as well as high overall mean exclusion chance values in trios (0.999 992) and in duos (0.99). Conclusion InDel X-18PLEX meets the requirements as a forensic DNA complementary kit, providing effective supplementary analytical tools for difficult cases.

To ascertain the optimal harvest period of Lonicera Flos (Lonicera macranthoides) the configuration yield and quality of L. macranthiodes bloom verity and bud verity flower at different develop periods were Observed. The quality of L. macranthiodes which harvested at different times of the day was Compared. The configuration was significant difference between different develop period of L. macranthiodes flower. As bud growth, yield increased. Bloom verity of L. macranthoides chlorogenic acid content was significantly lower after opening (silver flower stage, golden flower stage), before opening (young bud stage, green-white stage) have no significant difference of the quality. Bud verity of L. macranthoides macranthoidin B is significant lower at yellow-white stage, young bud stage and green-white stage have no significant difference of the quality. The chlorogenic acid and isochlorogenic acid A content is significant difference between L. macranthoides harvested at different time of the day. The optimal harvest period of bloom verity is the white stage, picking time for 10:00 before and after 18:00. The optimal harvest period is the green-white stage, picking time is 8:00 before and after 18:00.
Agriculture ; Drugs, Chinese Herbal ; analysis ; Flowers ; chemistry ; growth & development ; Lonicera ; chemistry ; growth & development ; Time Factors

Congresses as Topic ; Hearing Tests ; Humans ; Infant, Newborn ; Neonatal Screening

Objective To investigate expression of CD34 and platelet derived growth factor receptor ? (PDGFR-?) and pattern of DNA ploidy in dermatofibrosarcoma protuberans (DFSP), and to study their significance and relationship. Methods The expression of CD34 and PDGFR-? was detected by immunohistochemical SP method in paraffin-embedded specimens from 39 DFSPs and 30 dermatofibromas (DFs). The findings were quantitatively analyzed by image system. The pattern of DNA ploidy was detected by flow cytometry. Results The expression of CD34 and PDGFR-? in the DFSPs was higher than that in the DFs (P

Resveratrol, an important phytoalexin, has a variety of physiological activities in the treatment of diseases and in health care. In recent years, it has been extensive and in-depth researched in the field of plant sources, food and pharmaceutical industry, plant physiology, and so on. The natural plant resources which contain resveratrol are included in this article. The advances in study on resveratrol are reviewed, which includes the methods of extraction, detection, synthesis, metabolism, absorption, distribution, and pharmacological action, to provide the valuable reference for researching and developing resveratrol.