This paper describes the characteristics of post-apoplectic talipes varus at the early and middle-late stages and puts forward the acupuncture and rehabilita -tion-therapy.

Objective: To discuss the therapeutic method of apoplectic patients with language disorders.Methods: Sixty cases of apoplexy were classified into electroacupuncture group of 30 cases and acupuncture group of 30 cases. In electroacupuncture group, acupoints Lianquan (CV 23), Tongli (HT 5) and lower 2/5 part of motor area and sensory area were selected. In acupunc -ture group, acupoints Lianquan (CV 23) and Tongli (HT 5) were selected. Results and conclusions: After 2 courses of treatment, the total effective rate in electroacu -puncture group was 93.3% and that in acupuncture group was 76.6%. There was a significant difference in the therapeutic effect between the two groups (P＜0.05).

AIM: To investigate the therapeutic effects of the marine capsules combined with composite Salvia miltiorrhiza dropping pill in treatment with patients with hepatic fibrosis related to chronic hepatitis B. METHODS: 54 patients were enrolled and randomly divided into control group and treatment group. The liver function,the marker of hepatic fibrosis and hepatitis B virus were determined before and after therapy. RESULTS: The markers in this study were significantly improved than those before treatment and those in control group (P

Seventy-six athletes with acute lumbar sprain were treated by Tuina plus Acupuncture. First the acupoints of Bladder Meridian and Governor Vessel were Tuina to promote the recovery of lumbar muscles. Then Shuigou (GV 26), Weizhong (BL 40) and Fuyang (BL 59) were punctured. After 5 treatments, 46 cases were cured, 20 cases got remarkable effectiveness, 8 cases got effectiveness and 2 cases failed with the total effective rate of 97.4%.

Objective To explore the effect of direct observation of procedural skills (DOPS) in teaching acupuncture manipulation skills. Methods Sophomores of acupuncture and Tuina spe-ciality were divided into two groups based on their classes, 36 students in experimental group and 31 in control group. In experimental group, DOPS on filiform needling was done twice in the middle and at the end of the semester respectively. In control group, DOPS was done once at the end of semester. The final exam's DOPS score and satisfaction degree of teachers and students concerning the results of the assessment in two groups were compared by independent samples t-test. P<0.05 indicates signifi-cant differences. The teaching effect of DOPS was also evaluated by questionnaire survey of students in experimental group. Results The total score of DOPS (4.26 ±0.24), scores of needle insertion (4.18±0.42), and scores of reinforcing and reducing method (4.03±0.28) in experimental group were higher than those in control group ((4.14±0.25), (4.00±0.29) and (3.87±0.37), P<0.05). The teachers' satisfaction (8.38±0.51) and students' satisfaction (8.47±1.03) concerning the results of the assessment in experimental group were higher than those in control group ((8.05±0.78) and (7.94±1.15), P<0.05). More than 80% of students in experimental group supported the saying of‘DOPS promoting students' skills training and enhancing the ability of handwork ’ . Conclusions DOPS can be used as an effective teaching and examination method and can improve students' skills of acupuncture manipulation.

Objective To explore the effect of flipped classroom in teaching auricular needling method. Methods Sophomores of acupuncture and Tuina speciality were divided into two groups based on classes, with 33 students in experimental group and 30 in control group. In teaching auricular needling method, experimental group adopted flipped classroom by online resources and classroom activities, while control group adopted traditional classroom such as teaching, watching video, demonstration. Post-test score of two groups were compared by independent samples t-test. The teaching effect of flipped classroom was evaluated by questionnaire survey of students in experimental group. Results The total score of auricular needling (88.4±13.91), score of location (55.40±7.34), and score of application (32.00±10.31) in experi-mental group were significantly higher than those in con-trol group (71.48±15.93, 47.55±12.23 and 23.94± 11.97) (P<0.05). Students' satisfaction concerning the flipped classroom in experimental group was up to 90%. Conclusion Flipped classroom can be used in teaching auricular needling method, which can improve teaching quality and promote improvement of students' self-study and communication skills.

Filiform needle acupuncture technique is the core content of needling and moxibustion method. The examination and assessment of filiform needle manipulation are the most important measure for testing the effect of filiform needle acupuncture technique teaching. The establishment of a scientific examination and assessment system of filiform needle manipulation should involve comprehensive examination and assessment contents, definite and detailed evaluation rules and quantified scoring criteria to make the examination and assessment system be able to comprehensively and objectively assess the students' skills of filiform needle manipulation to achieve the purpose of improving the quality of filiform needle acupuncture technique teaching.

Objective To investigate the molecular epidemiology and mechanism of carbapenem resistance of Escherichia coli collected from intensive care units(ICUs)of general surgery.Methods Agardilution were carried out to confirmed the drug-susceptibility,pulsed-field gel electrophoresis(PFGE)were performed to analyze the molecular epidcmiology of carbapenem-resistance isolates.Specific PCR,DNA sequencing,conjugation experiments,plasmids extraction,plasmid transformation assays and SDS-PAGE of outer membrane proteins(OMPs)were carried to confirm genotype of carbapenemase and its transmission mechanism.Results PFGE showed the isolates belonged to 10 clonotype,and all the clinical isolates were resistant to β-lactams including imipenem and meropenem,but uncertain to aminoglycosides,specific PCR and DNA sequencing revealed that all isolates encoded carbapenem-hydrolyzing enzyme gene,KPC-2.Plasmid DNA extraction and plasmid transformation assays from some isolates comfirmed that KPC-2 encoded on a 56 kb plasmid.SDS-PAGE analysis confirmed that there are alterations in OMPs of Escherichia coli.Conclusion Escherichia coli isolates with carbapenem resistance are collected from our hospital,production of KPC-2 carbapenemase mainly contributed to reduced susceptibility of carbapenem in Escherichia coli,the alterations in OMPs may as a cofactor in high-level drug-resistance in Escherichia coli.

Adolescent ; Humans ; Male ; Neurofibroma, Plexiform ; Neurofibromatoses ; Parotid Neoplasms

BALB/C mice were immunized by intraperitoneal injection with A-group erythrocytes, B-group erythrocytes and saliva of A-group,B-group secretor. Immune spleen cells were fused with sp2/0 murine myeloma cells. The supernatants from each well were examined for antibodies by hemagglutination using A-,B-, O-group RBC.3 hybridoma cell lines of secreting anti-A antibodies and 3 hybridoma cell lines of secreting anti-B antibodies were established.These hybridoma cells have maintained and produced antibodies possessing higher specificity and titer.The agglutination titer of supernatants reached 2048-fold. These antibodies were used in the field of forensic science.