Objective To construct and expr es s a recombinant plasmid of nonstructural protein NS3-NS4 of hepatitis C virus ( HCV), and to identify the antigenicity of the expressed protein. Methods A gene region encompassing the nonstructural protei n NS3-NS4 of HCV was amplified by polymerase chain reaction (PCR) from the pUC1 9/HCV template. The recombinant expression plasmid containing the pBV220/NS3-NS 4 sequence was constructed, and the nonfused NS3-NS4 recombinant protein was ex pressed in E.coli DH5? efficiently. The recombinant protein was det ected by SDS-PAGE and ELISA. Results We successfully constructed and expressed the recom binant plasmid in prokaryote. Its antigenicity was detected with 50 standard ser a. Compared with the second-generation diagnostic Kit, the total detection rate was 96%. Conclusion The whole NS3-NS4 protein, a region of dominant immunogenicity, should be the effective component of the HCV diagnostic Kit and provide the clue for developing HCV DNA vaccine.

Intravenous urography is very important in the detection of pathological conditions of the urinary tract, but the presence of intestinal gas shadows may be very confusing in the interpretation of urograms. There is considerable difference of opinion regarding the desirability of preparing patients for excretory urography. we obtained clinical impression and statistical results that intestinal gas was increased with prolonged duration after last diarrhea, also with sleeping after last diarrhea. So we attempted one new method of preparation in which employed 30cc, of castor oil for catharsis two times at night before examination and next morning 6:00 a. m. and result was compared with other methods. A total of 300 cases, for the most in-patients, consisted of 6 groups, were studied in this report. All patients had nothing by mouth after midnight until the X-rays were completed and pyelograms usually were taken in the morning. Following methods of preparation were studied. Group 1 : 60 cc. castor oil at bed time. Group 2 : 60 cc. castor oil at 4:00 a. m. Group 3 : 30 cc. castor oil at bed time and 30 cc. at 6:00 a. m. Group 4 : 60 cc. castor oil at bed time and enema in the morning. Group 5 : No cathartics and enema. Group 6 : Dulcolax 2 tablets at bed time. The X-ray films were graded as A) excellent B) good C) fair D) poor by three or more examiners. Group 3. afforded best result. We believed that result was due to prevention of intestinal fermentation by ingestion of castor oil at bed time and passage of swallowed air before X-ray examination by repeated ingestion at 6:00 a. m. A larger series of cases over a longer period of time, for more accurate statistical study, is warranted.
Bisacodyl ; Castor Oil ; Catharsis ; Cathartics ; Diarrhea ; Eating ; Enema ; Fermentation ; Humans ; Mouth ; Statistics as Topic ; Tablets ; Urinary Tract ; Urography* ; X-Ray Film

The lymphatic system is of considerable importance in the spread of malignant tumors. Lymphography provides a simple and direct radiological method of demonstrating clinically unsuspected metastases in lymph nodes. Our study was intended to assess the role of lymphography in the management of the genitourinary tract tumors. The following results were obtained 1) It is helpful in the assessment of plaints with carcinoma of the penis, who have inguinal metastasis and are under consideration of inguinal lymphadenectomy. 2) It is helpful in determining the radiation portals. 3) It is helpful in deciding the stage of disease and in expecting the prognosis. 4) It is superior to IVP in detecting retroperitoneal metastasis. 5) It is valuable in confirming the completeness of lymphadenectomy. 6) It is helpful in reviewing the response to radiotherapy and chemotherapy, and also in the evaluation of recurrence within one year.
Anti-Bacterial Agents* ; Catheters ; Drug Therapy ; Lymph Node Excision ; Lymph Nodes ; Lymphatic System ; Lymphography ; Male ; Neoplasm Metastasis ; Penis ; Prognosis ; Radiotherapy ; Recurrence ; Urinary Tract Infections* ; Urinary Tract*

Recently we experienced 10 cases of posterior urethral injury associated with pelvic fracture. Of these patients 5 underwent primary realignment with perineal traction on the prostate (Selikowitz method), and the other 5 underwent immediate cystostomy and delayed urethroplasty (Johanson method). Comparison of the post-operative complications didn`t appear to show significant differences in the 2 groups, although the numbers were small. But patients who performed immediate cystostomy and delayed urethroplasty showed some disadvantages of the long period of treatment, high charges, often multiple hard procedures and the long time of operation. Therefore in the initial management of posterior urethral injury, we think it better to take primary realignment in Korean socioeconomic circumstances, if the patient is not in the high risk.
Cystostomy ; Humans ; Prostate ; Traction

A clinical observation on the nephrectomized 114 patients in the department of Urology, Han Gang Sung Sim Hospital, during the period from January, 1972 to December, 1979 was presented with brief review of the literature. The result were as follows: 1. The total number of in-patients during above period was estimated to 1492. and nephrectomies were performed in the 114 of the 913 urologic operations. (12.4%) 2. The age distribution ranged from 1 year to 66 years with the most common age groups in the 4 th decades. (22.8%) 3. The ratio of male to female was 1.2 to 1. 4. Among the symptoms on admission, hematuria was in 82 cases (71.9%), flank pain in 74 cases (64.9%), and urinary frequency in 46 cases. (40.4%) 5. The causative diseases of the operated kidney showed the highest in renal tuberculosis (30.7%), renal tumor (18.4%), calculous disease (17.5%). renal injury (14.9%) and hydronephrosis (10.5%) by sequence. 6. Flank approach with 1 rib resection was the most frequently used in 52 of 114 cases. 7. Postoperative complications were seen with 3 cases of wound infection, 2 cases of wound dehiscence, 2 cases of atelectasis, 1 case of pneumonia, and 1 case of paralytic ileus. 8. 110 cases (96%) were discharged within 2 weeks after surgery.
Age Distribution ; Female ; Flank Pain ; Hematuria ; Humans ; Hydronephrosis ; Intestinal Pseudo-Obstruction ; Kidney ; Male ; Nephrectomy ; Pneumonia ; Postoperative Complications ; Pulmonary Atelectasis ; Ribs ; Tuberculosis ; Tuberculosis, Renal ; Urology ; Wound Infection ; Wounds and Injuries

The renal scan was performed of 18 cases of acute pyelonephritis. 2 cases were normal and 16 cases were abnormal uptake findings in renal scan. Among these cases, 11 cases were mottled, 4 cases were decreased, 1 case was defect uptake finding. Conclusively, we believe that renal scan is a more sensitive index than the pyelogram for renal parenchymal lesions in the acute pyelonephritis.
Pyelonephritis*

A clinical observation was made on 23 patients of renal staghorn calculi which were treated at the Department of Urology, Hangang sacred Heart Hospital, Hallym College. The results were as follows: 1. Patients ranged in age from 17 to 62 years and there were 12 males and 11 females, giving a male to female ratio of approximated 1.1 to 1. The right kidney was involved more than the left. Eleven patients (47.8%) had multiple renal calculi and 1 of the 23 patients had bilateral renal staghorn calculi. 2. As for methods of surgery, extended pyelolithotomy in 9 cases, nephrolithotomy in 5 cases, nephrolithotomy with partial nephrectomy in 1 case and nephrectomy in 8 cases were performed. 3. Postoperative complications included 3 cases of residual stone and 3 cases of urinary infection. 4. Recommendable surgical method for renal staghorn calculi is the preservation of kidney according to operation technique.
Calculi* ; Female ; Heart ; Humans ; Kidney Calculi ; Kidney* ; Male ; Nephrectomy ; Postoperative Complications ; Urology

A clinical observation was made on 23 patients of renal staghorn calculi which were treated at the Department of Urology, Hangang sacred Heart Hospital, Hallym College. The results were as follows: 1. Patients ranged in age from 17 to 62 years and there were 12 males and 11 females, giving a male to female ratio of approximated 1.1 to 1. The right kidney was involved more than the left. Eleven patients (47.8%) had multiple renal calculi and 1 of the 23 patients had bilateral renal staghorn calculi. 2. As for methods of surgery, extended pyelolithotomy in 9 cases, nephrolithotomy in 5 cases, nephrolithotomy with partial nephrectomy in 1 case and nephrectomy in 8 cases were performed. 3. Postoperative complications included 3 cases of residual stone and 3 cases of urinary infection. 4. Recommendable surgical method for renal staghorn calculi is the preservation of kidney according to operation technique.
Calculi* ; Female ; Heart ; Humans ; Kidney Calculi ; Kidney* ; Male ; Nephrectomy ; Postoperative Complications ; Urology

Objective To probe into effective surgical methods to reduce post-operational recurrence rate of nodular goiter.Methods One hundred and fifty-seven patients with nodular goiter who were admitted and had operations in our hospitals between Jan,2007 and June,2011 were assigned to one of the following 4 groups:total thyroidectomy(TT),nearly total thyroidectomy (NTT),subtotal thyroidectomy (STT),removal of lesion only or partial thyroidectomy (also termed as local removal,LR).Postsurgical recurrences and complications were compared among these groups.Results The recurrences were statistically analysed.There was a highly significant difference between TF and STT、TT and LR、NTT and LR; no difference was observed between TT and NTT,STT and STT + E,STT and LR.A statistical analysis was also carried out on the postsurgical compications in these patients.highly significant differnce was found between TT and STT,TT and NTT,TT and LR.There was a significant diffrence between STT and NTT.There was no difference between STT and LR,NTT and LR.Conclusion TT,STT,NTT are all effective operations for nodular goiter and should be advocated.Satisfactory efficacy may be obtained with oral thyroxine or euthyrox for postsurgical lifetime.However,LR or partial thyroidectomy should be adopted cautiously.

Octapeptin has strong antibacterial activity against Gram-negative bacteria such as Escherichia coli, Klebsiella pneumoniae and Acinetobacter baumannii, while it also has activity against some Gram-positive bacteria. This study used natural octapeptin A3 and B3 as lead compounds for structural modification. Twenty-one peptide derivatives (including A3 and B3) containing eight amino acid residues were prepared by solid-phase synthesis, and evaluated for antibacterial activity and renal cytotoxicity. Among them, three compounds 6, 7 and 17 exhibited broad-spectrum antibacterial activity and significantly enhanced the activity for Gram-positive bacteria while maintaining the activity of Gram-negative bacteria. Several compounds improved the activity for Pseudomonas aeruginosa. Compound 7 was active against all test strains and had relatively low renal cytotoxicity. The results provide a basis for the further development of novel polypeptide antibiotics.