Seasonal influenza is a common respiratory infectious disease with high morbidity and strong infectivity, and it is harmful to people. Influenza vaccination is an effective measure to prevent influenza and reduce the spread of the influenza. People who are in great risk of influenza or tend to have adverse reactions should be considered as a special population, including the young child less than 2 years, the men or women older than 65 years, children with egg allergy and pregnant women. The attitudes about the safety and efficacy of influenza vaccine in special populations should be changed following the improvement of the influenza vaccine production and the deeply research. This paper reviewed the production of common flu vaccines and the safety of influenza vaccination in special populations.

Fructose-1,6-bisphosphatase(FBPase) deficiency is a rare inherited metabolic disease,which is an autosomal recessive metabolic disorder.Affected patients usually present with metabolic crisis including severe hypoglycemia and metabolic acidosis.Each attack occurred with a similar sequence.The triggering factors are removed and then clinical status is improved dramatically.As patients are usually symptomless in the plateau stage,it is often misdiagnosed.Metabolite assay in blood and urine is very useful for the diagnosis of FBPase deficiency.FBPase is a key enzyme in gluconeogenesis.Deficiency of FBPase impairs the formation of glucose from all precursors.FBP1 gene mutation contributes to the disease.More than 30 mutation types have been reported.There is no specific treatment.Early diagnosis and appropriate life-style can prevent repetitive metabolic derangements,improving life quality of these children and ensuring successful pregnancy.

Objective To investigate the application effect of the health care integration mode after percutaneous coronary intervention (PCI) of patients in cardiology department.Methods 100 patients (the experimental group) were randomly selected from 200 patients to implement integrated health service management model,usual care service mode was given to the other 100 patients (the control group).After 12 months,the incidence of haemorrhage and hematoma,the average length of hospital stay,the patients' quality of life,patients' satisfaction degree in two groups of patients were compared.Results Compared with the control group,the incidence of postoperative haemorrhage and hematoma decreased,the average length of stay shortened,the patients' quality of life and satisfaction degree significantly improved after PCI in the experimental group.Conclusions Compared with ordinary care model,health integration model can reduce the incidence of haemorrhage and hematoma in patients after PCI,alleviate the suffering of patients; shorten the average length of stay and reduce the economic and emotional burden of patients; improve the patients' quality of life and satisfaction degree,deepening nursing work connotation,and promote the development of nursing.

Cytokines can have either pro-or anti-inflammatory activity and immunosuppressive activity,depending on the microenvironment.Tumors contain immune cells and a network of pro-and anti-inflammatory cytokines,which collaborate in the development and progression of cancer.Cytokine might prove to be prognostic.The systemic effects of pro-inflammatory cytokines are associated with fatigue,depression,cognitive impairment,anorexia,cachexia,pain,toxicity of treatment and resistance to treatment,which can affect quality of life.

Objective To investigate the influence of the establishment of Chest Pain Center (CPC) on the reperfusion treatment and prognosis of patients with acute ST segment elevated myocardial infarction (STEMI).Methods One hundred and eighteen patients with acute STEMI admitted into the Department of Emergency in Weifang People's Hospital from March to May 2016 before the establishment of the CPC were assigned as a control group,and 112 patients with STEMI admitted from September to November 2016 after the establishment of CPC were assigned as a study group.The first electrocardiograph (ECG) of all emergency patients was finished by nurses in the control group,after the cardiology physicians of Emergency Department having examined the patients,those with definite diagnosis of acute STEMI were sent into a resuscitation room immediately,and a loading dose of aspirin 300 mg and ticagrel 180 mg were given orally to each of the patients.The patients who accepted the primary percutaneous coronary intervention (PPCI) were transferred into a catheterization room as soon as possible;the patients who could not accept the PPCI,after the exclusion of contraindications of thrombolysis,were transferred into the emergency intensive care unit (EICU) to receive the intravenous thrombolytic treatment.For the study group,a uniform training was implemented,including the ECG interpretation,the diagnosis and treatment processes of chest pain for all of the medical staff,and establishment of a CPC database.The patients who were diagnosed as acute STEMI entered into the green channel of chest pain.For the patients who accepted the PPCI,the catheterization room was started immediately.The patients who could not accept the PPCI received the intravenous thrombolysis treatment:intravenous drip of urokinase 1 500 kU dissolved in 100 mL physiological saline was finished in 30 minutes.After treatment,the differences in the time from symptom onset to first medical contact (SO-to-FMC),the finished time of the first ECG after admission,the time of visiting doctor to reperfusion treatment[beginning of thrombolysis or ballon dilatation (DTRT)] including the door-to-needle (D2N) time or the doorto-balloon (D2B) time,the incidence of heart failure during hospitalization,the left ventricular ejection fraction (LVEF) measured with color Doppler ultrasound on the 7th day after admission and the in-hospital mortality were compared between the two groups of patients.Results There was no statistically significant difference between the study and control groups in the SO-to-FMC time (minutes:140.3 ± 108.4 vs.139.4 ± 112.7,P ＞ 0.05).The finished time of the first ECG after admission in the study group was significantly shorter than that of the control group (minutes:7.7 ± 1.3 vs.8.9 ± 1.7,P ＜ 0.05),the door to reperfusion time in the study group was also significantly shorter than that of the control group [D2B (minutes):72 ± 13 vs.83 ± 17,D2N (minutes):27 ± 9 vs.33 ± 12,both P ＜ 0.01].The incidence of heart failure during hospitalization of the study group was significantly lower than that of the control group [40.2％ (45/112) vs.53.4％ (63/11 8)].The left ventricular ejection fraction (LVEF) measured at one week after admission in study group was significantly higher than that of the control group (0.54 ± 0.05 vs.0.53 ± 0.04,P ＜ 0.01).The in-hospital mortality of the study group was lower than that of the control group [9.8％ (11/112) vs.14.4％ (17/118)],but there was no statistically significant difference between the two groups (P ＞ 0.05).Conclusion The application of the CPC run mode can further elevate the therapeutic level of reperfusion therapy,shorten the total ischemic time and improve the prognosis of patients with STEMI.

SUMMARY Peripheral nerve defects are still a major challenge in clinical practice,and the most com-monly used method of treatment for peripheral nerve defects is nerve transplantation,which has certain limitations and shortcomings,so new repair methods and techniques are needed.The peripheral nerve is elongated in limb lengthening surgery without injury,from which we got inspirations and proposed a new method to repair peripheral nerve defects:peripheral nerve elongation.The peripheral nerve could be elongated by a certain percent,but the physiological change and the maximum elongation range were still unknown.This study discussed the endurance,the physiological and pathological change of peripheral nerve elongation in detail,and got a lot of useful data.First,we developed peripheral nerve extender which could match the slow and even extension of peripheral nerve.Then,our animal experiment result confirmed that the peripheral nerve had better endurance for chronic elongation than that of acute elonga-tion and cleared the extensibility of peripheral nerve and the range of repair for peripheral nerve defects. Our result also revealed the histological basis and changed the rule for pathological physiology of peri-pheral nerve elongation:the most important structure foundation of peripheral nerve elongation was Fon-tana band,which was the coiling of nerve fibers under the epineurium,so peripheral nerve could be stretched for 8.5% -10.0% without injury because of the Fontana band.We confirmed that peripheral nerve extending technology could have the same repair effect as traditional nerve transplantation through animal experiments.Finally,we compared the clinical outcomes between nerve elongation and perfor-mance of the conventional method in the repair of short-distance transection injuries in human elbows, and the post-operative follow-up results demonstrated that early neurological function recovery was better in the nerve elongation group than in the conventional group.On the whole,all of these experimental re-sults revealed the physiological phenomenon of peripheral nerve elongation,and described the physiologi-cal change and stretch range in detail.The systematic research results have filled the blank in this field, which is very helpful for clinical limb lengthening surgery,the design of elongation surgery and the evalu-ation of the peripheral nerve stretch injury.Peripheral nerve elongation will become an innovative treat-ment technology in repairing peripheral nerve defects.

OBJECTIVETo observe the differences in the clinical efficacy on menopausal irritability between acupuncture and medication.

METHODSSixty cases of menopausal irritability were randomized into an acupuncture group (31 cases) and a medication group (29 cases). In the acupuncture group, the warming needling manipulation was applied at the key acupoints such as Hegu (LI 4) and Taichong (LR 3), once a day, 10 treatments made one session. Totally, 2 sessions were required. In the medication group, 2.5 mg diazepam tablets were prescribed, three times a day, for 23 days totally. The clinical efficacy, the modified Kupperman score and self-rated health measurement scale (SRHMS) were compared before and after treatment in the two groups. RESULTS The total effective rate was 87. 1% (27/31) in the acupuncture group and 82.8% (24/29) in the medication group. The difference in the clinical efficacy was not significant between the two groups (P > 0.05). The modified Kupperman score and SRHMS score were both reduced obviously after treatment in the two groups, indicating the significant difference (all P < 0.05). The reduction of two scores in the acupuncture group was more obvious than those in the medication group (Kupperman: 15.23 +/- 6.19 vs 18.45 +/- 5.37; SRHMS: 116.29 +/- 38.24 vs 140.34 +/- 42.15, both P < 0.05).

CONCLUSIONThe acupuncture and medication are effective in the treatment of menopausal irritability, but the efficacy of warming needling manipulation is better than that of diazepam tablets.

Acupuncture Therapy ; Adult ; Anti-Anxiety Agents ; administration & dosage ; Anxiety ; drug therapy ; psychology ; therapy ; Diazepam ; administration & dosage ; Female ; Humans ; Menopause ; drug effects ; psychology ; Middle Aged ; Treatment Outcome

Capillary gas chromatographic column 1 (7.5%　W/W,3 5×0. 35mm i.d.) and column 2 (9.2% W/W,35×0.30mm i.d.) using butylammonium 4-to unesulfonate as stationary phase were prepared by the method of dynamic coating with mercury plug, and the solvent used was mixture of acetone and methanol (2 ∶1　V/V). The results　showed that the column had good chromatograpic pro perties, especially to strong polar compound, such as carboxylic acids and acoho ls could be separated well.

Objective To investigate the dynamic change and different apoptosis of T lymphocyte subsets in patients with acute pancreatitis.Methods Forty-four patients with MAP and 32 with SAP were enrolled in this study.Blood samples were collected on the lst,3rd and 7th d after onset.T lymphocyte subsets and their apoptotic rate were determined by flow cytometry.Results Compared with the control group and MAP group,CD4+ T lymphocytes and the ratio of CD4+/CD8+remarkably decreased while the apoptotic rate of CD4+ T cells significantly increased in patients with SAP.However,there was no marked difference in apoptotic rate of CD8+ T cells among different groups.Conclusion The different apoptosis of T lymphocyte subsets in AP,especially in SAP,results in severe and sustained cellular immune suppression.

Objectives: To explore the destructive mechanism of P.gingivalis on periodontium and to better understand the pathogenic effects of P.gingivalis fimbriae. Methods:Western blotting was used to detect the degradation effects of P.gingivalis ATCC 33277 and its fimA-deficient mutant on focal adhesion components paxillin and focal adhesion kinase (FAK) of Hela cells and immortalized gingival epithelial cells (IHGE cells). Results:P.gingivalis ATCC 33277 wild strain and its fimA-deficient mutant induced degradation of paxillin and FAK both in Hela cells and in IHGE cells. fimA-deficient mutant had a remarkable weaker degradation ability than the wild strain. In IHGE cells, paxillin and FAK were degraded in a time-and MOI-dependent manner. Conclusion:P.gingivalis fimbriae-mediated adhesion and invasion to epithelial cells may promote the degradation of focal adhesion components. IHGE cells may be more suitable for the study of periodontal pathogens than Hela cells.