DNA quantification is important to ensure the consistency and the reliability in the interpretation of degraded low copy number DNA typing. We applied the simple PCR quantification method using fluo-rescently labeled primers for the amplification of mtDNA and amelogenin gene in 50 year old skeletal remains (e.g. bone and tooth). K562 DNA was serially diluted and used as a standard for concentration marker to gauge the amount of DNA from PCR versus the peak area. The quantities of DNA extracted from bones and teeth did not show significant difference in the analyses both using mtDNA and amelo-genin gene as an amplification target. To test the efficiency of DNA profiling of degraded low copy number DNA samples, mtDNA PCR quality evaluation and DNA typing for 16 autosomal STR and 9 Y chromosomal STR loci were per-formed and the correlation between DNA quantities and PCR amplification efficiencies of the samples was analyzed. The DNA quantities assayed by the simple method suggested in the present study could be good indicator for mtDNA and STR analysis. As the allele drop-out was observed in less than 0.050ng DNA samples, at least 0.100ng of DNA is required to produce informative STR profiles. Also, STRs with less than 200bp amplification sizes produce efficient DNA profiles in most cases. Therefore, the develop-ment of mini-STRs with less than 200bp amplification sizes is expected to improve DNA typing in degraded low copy number DNA. Y-STRs are easy to detect allele drop-out or drop-in, and accordingly the efficiency test of Y-STRs as well as autosomal STRs for profiling of degraded low copy number DNA samples is thought to be important.
Alleles ; Amelogenin ; DNA Fingerprinting* ; DNA* ; DNA, Mitochondrial ; Humans ; Middle Aged ; Polymerase Chain Reaction ; Tooth

A population study of the X-chromosomal short tandem repeat (STR) loci DXS9898, DXS6809, DXS7424 and DXS10011 was carried out by single multiplex PCR in a sample of 300 unrelated Korean individuals (150 males and 150 females). For accurate and reproducible STR typing, sequenced allelic ladders were constructed and GenoTyper macro was programmed. In this study, four types of the repre-sentative repeat sequence structure of DXS10011 were observed and the allele loss at DXS9898 was observed in 13 of 450 chromosomes (2.9%). The inter-population comparison of the allele frequencies at the 4 X-STRs showed significantly different distributions (p<0.01) for Koreans and Germans except DXS10011. All statistical parameters for forensic efficiency showed that the 4 X-chromosomal STRs are highly informative. Especially, DXS10011 is expected to be the most useful marker for forensic practice.
Alleles ; Gene Frequency ; Humans ; Male ; Microsatellite Repeats ; Multiplex Polymerase Chain Reaction

The study presents the application of STR polymorphisms to male identification in the cases of a serial rapist in Korea. Since October 2000 a rapist from Cheongju city (a town located in central district) com-mitted at least 36 rapes. DNA profiles obtained from semen stains, buccal cells and tongue tissue left at the scenes of crime and vaginal swabs of victims gave information that one and the same man had com-mitted all the rapes. The Y-chromosome haplotype (12 loci) obtained was used for the identification process of rapist in mixed genotypes including victim's alleles. The crime scene data were referred from investigating police authorities described the relationship of victim to the offender, victim's age, time and place of sexual assault and rape locations. The behavioural analysis of crime scenes showed more similarities than differences for related crimes. These components were usually assessed in terms of proximity in time and space. Certain crime scene aspects of a serial rapist help identify components of offender spatial behaviour. As a result of this work, future research on the geography of rape and behav-ioural aspects of rapist will be directed towards those aspects of offences, which have been identified as relevant to the distance between an offender's base, and the site where he approached his victim.
Alleles ; Chungcheongbuk-do ; Coloring Agents ; Crime* ; Criminals ; DNA ; Genotype ; Geography ; Haplotypes ; Humans ; Korea ; Male* ; Police ; Rape ; Semen ; Tongue

The autopsy system in Korea is based on the Corpse Autopsy and Preservation Law which Articles 2 didn 't limit medical professors and medical examiners to perform autopsy. But according to the Medical practitioners Law Article 18, only a practicing physician can issue the certificate for postmortem inspec-tion. If an unnatural death is suspected, the postmortem investigation and autopsy should be performed according to the order of Criminal Procedure Code Article 222. The limited role of postmortem investi-gation in unnatural death and respective bodies of postmortem inspection and autopsy, which would cause the overflow of criminal autopsy. The detailed guide-line of unnatural death, should-be verified death cases, practical use of postmortem inspection including the blood and tissue sampling etc, unifica-tion of legislations regarding to autopsy system, the specific warrant of autopsy, which empowers the limited and full autopsy individually would be help to improve that distortion.
Autopsy* ; Cadaver ; Coroners and Medical Examiners ; Criminals ; Humans ; Jurisprudence ; Korea

A simplified method of the cardiac conduction system (CCS) is evaluated by the study of 73 forensic cases. The sinoatrial node were observed in all cases (100.0%), atrioventricular node in 70 cases (95.9%) and penetrating bundle (His bundle) in 70 cases (95.9%). We divided the cases into three groups as the group of cardiac death (25 cases), non-cardiac death (24cases) and sudden unexpected death but undeterminable cause of death (24 cases) diagnosed after routine autopsy including routine cardiac examination and toxicological analysis and compared the type and incidence of CCS lesions in each groups. Narrowing of the sinoatrial (SA) or atrioventricular (AV) artery by fibromuscular hyperplasia (FMH) and fatty infiltration in SA or AV node were identified in all groups, lymphocytic infiltration in SA or AN node in cardiac and non-cardiac death group, hemorrhage and fibrosis of SA or AV node were identified in cardiac death group. FMH of the artery of SA or AN node occured more commonly in undeterminable cause of death group (45.1%) than in cardiac (16.0%) and non-cardiac group (20.8%) and severe narrowing (> or =75%) of the artery of SA or AV node was only present in undeterminable cause of death group (29.2%). The results led to the conclusion that this simple technique is very useful in detection of major abnor-malities of CCS with minimal effort and examination of the CCS in death which the routine autopsy and drug screen fail to provide a cause of death can yield a cause of death in a significant percentage of cases.
Arteries ; Atrioventricular Node ; Autopsy ; Cause of Death ; Death ; Death, Sudden* ; Fibrosis ; Hemorrhage ; Hyperplasia ; Incidence ; Sinoatrial Node

We reviewed forensic autopsies and scene examinations performed at Central District of National Institute of Scientific Investigation, requested by every kinds of law enforcement agencies in Chung-cheong area of Korea during the year of 2003, and analysed the data of forensic autopsies and scene examinations according to the cause of death and the manner of death. The results are as follows ; 1. Scene investigation on the spot was performed in 220 cases ; Among these cases, 161 cases (73.2%) were unnatural deaths and the most common cause of death was trauma (61 cases). Autopy was per-formed in 93 cases (42.3%). 2. The total number of forensic autopsy were 685 cases (452 males and 233 females). The number of the forties (215 cases) and the thirties (124 cases) occupied almost half (49.5%) of all cases of autopsy. 3. Unnatural deaths were 434 cases (63.3%), natural 204 cases (29.8%), and the unknown 47 cases (6.9%). In 434 cases of unnatural deaths, suicide was 84 cases (19.3%), homicide 115 cases (26.5%), acci-dent 186 cases (42.9%), and the undetermined 49 cases (11.3%). Homicide occupied 43.7% of trauma, 77.1% of blunt force injury excluding traffic and fall down injury, and 97.7% of sharp force injury. The homicide rate in asphyxia was 35.0%. It showed only 1 cases (1.9%) of homicide in poisoning. 4. In 434 cases of unnatural deaths, traumatic deaths were 229 cases (52.8%). Blunt trauma was 70 cases (30.6%) and the leading cause of death in traumatic death. Of remaining 205 cases of unnatural deaths, there were 52 cases (12.0%) of poisonings, 51 cases (11.8%) of thermal injuries, 41 cases (9.4%) of drowning, 40 cases (9.2%) of asphyxia, 19 cases (4.4%) of medical procedures, and 2 cases (0.5%) of electrocution. 5. Among 204 natural deaths, cardiovascular diseases were 106 cases (52.0%) and the leading cause of death in natural deaths. 36 cases (17.6%) of diseases involving vascular system were followed. 6. Child deaths under the age of 10 were 32 cases. Among these cases, unnatural deaths were 14 cases (43.8%) and most of them were accident.
Asphyxia ; Autopsy* ; Cardiovascular Diseases ; Cause of Death ; Child ; Drowning ; Homicide ; Humans ; Korea* ; Law Enforcement ; Male ; Poisoning ; Suicide

No abstract available.
Agglutination* ; Animals ; Cats* ; Erythrocytes* ; Humans*

No abstract available.
Agglutination* ; Alisma* ; Animals ; Erythrocytes* ; Mice* ; Plantago*

No abstract available.
Euonymus*

No abstract available.
Animals ; Erythrocytes* ; Hemagglutination* ; Humans* ; Mice*