Ojective To research the appropriate routes the clinical results of the three different procedures for the laparoscopic adrenalectomy routes were compared. Methods Eighty six patients underwent laparoscopic adrenalectomy through three different routes-transperitoneal anterior (TAA), retroperitoneal lateral flank(RLA) and retroperitoneal posterior lumbar approaches(RPA). Results The time for performing operation through RPA and RLA was shorter than that through TAA(P

Objective To investigate the effects of salvianolic Acid A and B(Sal A,Sal B)on acute myocardial ischemia of rats.Method The rat model with acute myocardial ischemia was established by coronary ligation and intravenous injection of posterior pituitary.The effect of Sal A 10,5,2.5 mg/kg and Sal B 10 mg/kg on the electrocardiogram of different time points,serum creatine kinase(CK),lactate dehydrogenase(LDH)and myocardial infarction area were observed.Results To the rat model was established by coronary ligation,Sal A 10,5,2.5 mg/kg and Sal B 10 mg/kg could lower the ST-segment elevation,reduce infarct size and serum CPK,LDH.The effect of Sal A 10,5 mg/kg was significantly higher than that of Sal B.The role of Sal A 2.5 mg/kg was equivalent to Sal B 10 mg/kg.To the rat model was established by intravenous injection of posterior pituitary,Sal A 10,5,2.5 mg/kg and Sal B 10 mg/kg could lower the ST-segment elevation.The effect of Sal A 10,5 mg/kg was significantly higher than that of Sal B.The role of Sal A 2.5 mg/kg and sal B 10 mg/kg had no significant difference.Conclusion Treatment with Sal A 10,5 mg/kg was more effective in the rat models with acute myocardial ischemia than Sal B 10 mg/kg.Sal A 2.5 mg/kg improved acute myocardial ischemia in rats was equivalent to Sal B 10 mg/kg.

Objective To compare the safety and effectiveness of disposable circumcision suture device (DCSD) with conventional circumcision in surgical treatment of phimosis and redundant prepuce . Methods One hundred and sixty one patients with phimosis or redundant prepuce were randomly assigned to be treated by circumcision using disposable circumcision suture device ( DCSD group , n =89 ) or conventional circumcision (CC group,n =72),respectively.Results The operation time was (3.8 ± 0.5)min in DCSD group and (31.6 ±2.7)min in CC group (F=71.21,P=0.000),blood loss was 1.0(0.9, 1.2)ml in DCSD group and 6.2(5.7, 7.0)ml in CC group (Z =-10.90,P=0.000).There were no significant differences in intraoperative pain score (1.4 ±0.6 vs.2.9 ±0.8, F=2.08, P=0.151) and wound healing time [(14.0 ±1.3) vs.(16.8 ±2.1) d, F=0.10, P=0.754] between two groups.There was no need to change the dressing for patients in DSCD group 3 days after the operation , while patients in CC group changed dressing 3 times after the operation .No significant differences in postoperative complication rate [10%(9/89) vs.4%(3/72),χ2 =2.04,P=0.153] and satisfaction rate with the penile appearance [98%(87/89) vs.94%(68/72), χ2 =0.47,P=0.494] were found between two groups .Conclusion DCSD exhibits superiority over CC for shorter operation time and less blood loss in circumcision .

The clinical and pathological data of 6 patients with urahcal adenocar,cinoma of the bladder admitted in Beijing Luhe Hospital from 1996 to 2016 were analyzed and relevant literature was reviewed.Urachal carcinoma accounted for 0.66% of the bladder cancers admitted at the same period (6/906) and 6/16 of the bladder adenocarcinomas.Painless hematuria was the most common symptom.One patient had extensive metastasis as shown in biopsy and died 6 month after the diagnosis.Two patients underwent partial excision of bladder with no tumor recurrence.One patient underwent partial cystectomy followed by chemotherapy and radiotherapy, recurrence occurred one year later and he then received radical cystectomy.Another 2 patients underwent laparoscopic cystectomy and en bloc resection of umbilical ligament and umbilicus, and no recurrence was found in the follow-up for 6 years and 15 months, respectively.Pathological and immunohistochemistry are essential for the diagnosis of urachal adenocarcinoma.As it is not sensitive to radiotherapy or chemotherapy,surgery is the preferred treatment for patients with early stage diseases.Individual anticancer therapy may be the trend of treatment.

AIM:To investigate the kinetics of tumor necrosis factor alpha(TNF-?)in an animal model of chronic P.aeruginosa biomembrane associated with lung infection.METHODS:Rats were challenged with 0.1 mL of PAO579(1012 CFU/L)in alginate beads or 0.1 mL of planktonic PAO579(1012 CFU/L).After challenging for 3,7 and 14 d,bacteriological and pathological features,and TNF-? expression in lung tissue were observed.RESULTS:(1)CFU/lung in alginate beads group was significantly higher than that in planktonic bacteria group(P

Objective To evaluate the clinical value of interventional chemotherapy and intravesical instillation for preventing postoperative recurrence and metastasis of cancer in urinary bladder.Methods One hundred and eight patients with urinary bladder cancer were divided into group A and group B.Intravesical instillations after surgical operation were performed in group A (n=52) and combined interventional chemotherapy and intravesical instillations after operation were performed in group B (n=56).The patients were followed up for 1 - 3 years.Results In group A,recurrence occurred in 5 cases (9.62%) within one year,and in 23 cases (44.23%) within 3 years after the operations metastasis developed in 21 cases (40.38%),and 18 cases (34.62%) died.In group B,recurrence occurred in 2 cases (3.57%) within one year,and in 11 cases (19.64%) within 3 years after the operation;metastasis developed in 7 cases (12.50%), and 5 cases (8.93%) died.There were statistical significant differences in recurrence,metastasis and mortality between these two groups (P

E-cadherin is a key epithelial protein and adhesive molecule. This study detected the E-cadherin expression in patients with chronic rhinosinusitis with nasal polyps (CRSwNP) and controls, and analyzed its possible role in the pathogenesis of CRSwNP. The expression of E-cadherin was detected by using Western blotting and immunohistochemistry in controls and patients with CRSwNP. Computed tomography (CT) scan findings were scored. The results showed that the E-cadherin expression was up-regulated in patients with CRSwNP as compared with controls (P=0.039) and the positive staining was predominantly localized on the epithelial cell membrane. E-cadherin level was correlated negatively with Lund-Mackay scores in patients with CRSwNP (r=-0.604, P=0.005). It is suggested that E-cadherin may be involved in the pathogenesis of CRSwNP and correlated with disease severity.

The clinical data of 9 patients with huge adrenal tumors (≥6 cm) undergoing retroperitoneal laparoscopic adrenalectomy between February 2015 and September 2016 were retrospectively analyzed.There were 2 males and 7 females aged 29-65 years.The tumors were detected due to investigation of paroxysmal hypertension in 4 cases, and during the health check-up in 5 cases.The lesions were located on the right side in 4 cases, on the left side in 4 cases and bilaterally in 1 case.The operations were all successful, there was no case transferring to open surgery.The average operation time was 70 min (50-100 min),mean bleeding volum was 100 ml(50-200 ml).The postoperative diagnosis: 4 cases of pheochromocytoma, 2 cases of cortical adenoma, 2 cases of neurilemmoma and 1 case of neuroinoma.The average follow-up time was 13.5 months (6-24 months) and no recurrence was found.

Objective To observe the expression of cardiotrophin-1(CT-1) in ischemia-reinfusion cardiac muscle of rats and the effect of neuregulin-1(NRG-1).Methods The model of ischemia-reinfusion cardiac muscle of rats were prepared,35 rats were randomly divided into 4 groups:model group(n=8),NRG-1 pretreatment group(n=9),pseudo-surgery group(n=8) and normal control group(n=10).The CT-1 mRNA in the observed cardiac muscle of all groups was measured by RT-PCR and the relative amount of CT-1 mRNA were calculated,and for statistical treatment.Results The CT-1 mRNA of model group was(63.96?9.34),and it was higher than that of pseudo-surgery group(36.16?5.43)and normal control group(36.84?4.64).The significant differences were found in 3 groups(F=47.37 P

OBJECTIVE To understand the effects of lasR/rhlR mutations on the biofilm formation of Pseudomonas aeruginosa in vitro.METHODS The biofilms of PAEO1 and lasR,rhlR,and lasR rhlR mutants were incubated in a polyvinyl chloride tube in 0.9% saline and studied by means of scanning electronic microscopy(SEM) after three days′ incubation.RESULTS After 3 day′s incubation,PAEO1 biofilm showed a well-developed structure,whereas thin and poor developed biofilms were seen in lasR/rhlR mutant groups.CONCLUSIONS lasR/rhlR Genes play probably an important role in the formation of P.aeruginosa biofilm in vitro.