We conducted phylogenetic analyses and an estimation of coalescence times for East Asian strains of HTLV-1. Phylogenetic analyses showed that the following three lineages exist in Japan: “JPN”, primarily comprising Japanese isolates; “EAS”, comprising Japanese and two Chinese isolates, of which one originated from Chengdu and the other from Fujian; and “GLB1”, comprising isolates from various locations worldwide, including a few Japanese isolates. It was estimated that the JPN and EAS lineages originated as independent lineages approximately 3,900 and 6,000 years ago, respectively. Based on archaeological findings, the “Out of Sunda” hypothesis was recently proposed to clarify the source of the Jomon (early neolithic) cultures of Japan. According to this hypothesis, it is suggested that the arrival of neolithic people in Japan began approximately 10,000 years ago, with a second wave of immigrants arriving between 6,000 and 4,000 years ago, peaking at around 4,000 years ago. Estimated coalescence times of the EAS and JPN lineages place the origins of these lineages within this 6,000–4,000 year period, suggesting that HTLV-1 was introduced to Japan by neolithic immigrants, not Paleo-Mongoloids. Moreover, our data suggest that the other minor lineage, GLB1, may have been introduced to Japan by Africans accompanying European traders several centuries ago, during or after “The Age of Discovery.” Thus, the results of this study greatly increase our understanding of the origins and current distribution of HTLV-1 lineages in Japan and provide further insights into the ethno-epidemiology of HTLV-1.

The aim of the present study was to investigate the consciousness among young people (n = 494) of training body part(s) by showing participants a picture of an exercise, through a questionnaire-based survey. Participants were shown a picture of a Back Squat, and were asked which body part(s) they felt was being trained in the picture. Participants who had no experience of doing squatting exercises (NO-SQUAT group, n = 102) and participants who were experienced in terms of doing squatting exercises and weight training under appropriate supervision (INSTRUCTED group, n = 146) were selected for the analysis. On seeing the picture, more than half of the NO-SQUAT group felt that the Back Squat was to train not only their lower body but also another body part(s); among these participants, approximately 70% felt that a Back Squat was appropriate to train their upper torso and arms too. Further, the NO-SQUAT group had made significantly fewer attempts to consciously train their gluteal and knee flexor muscles, and had made significantly more attempts to consciously train their upper torso and arms during the squatting exercises than the INSTRUCTED group had. These results suggest that to do exercises by referring to only a picture may result in the participants not properly understanding how the body part(s) should be trained during exercise. Therefore, to enhance the benefits of training, individuals need to be instructed, under appropriate supervision, on correct technique as well as knowledge about the exercise.