1.Research advances in the characteristics and detection of Kobuviruses.
Hong-Bo DAI ; Song LI ; Yuan-Cheng ZHOU ; Ling ZHU ; Zhi-Wen XU
Chinese Journal of Virology 2012;28(5):591-594
In the present work, we reviewed the discovery, epidemiology, molecular biology and detection of Kobuvirus. Future fields of research were also discussed.
Animals
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Genome, Viral
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Humans
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Kobuvirus
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genetics
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isolation & purification
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Picornaviridae Infections
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epidemiology
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veterinary
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virology
2.Detection of Aichi virus in stool samples from children in Lanzhou.
Bo-Wen LI ; Sha LIANG ; Jin-Xin XIE ; Jie LI ; Bei LAN ; Yong-Qing LI ; Hui-Ying LI ; Wei-Xia CHENG ; Dan-Di LI ; Yu JIN ; Zhao-Jun DUAN
Chinese Journal of Experimental and Clinical Virology 2010;24(1):11-13
OBJECTIVETo identification and analysis Aichi virus from diarrhea and normal children in Lanzhou, and discuss the relationship between Aichi virus and Infant Diarrhea.
METHODSAccording to the literature published data, Using RT-PCR method to amplified Aichi virus 3CD fragment and the positive products were sequenced and determined, and made the alignment analysis between the nucleotide sequences of the amplified fragment with the known sequence of this virus.
RESULTSThere was 1 case detection of Aichi virus in the 46 hospitalized children with diarrhea and 299 children with diarrhea out-patients specifically, Overall detection rate was 0.06%, and there was no Aichi virus was detected in normal control children. 2 viral 3CD gene and the known reference strains of nucleotide sequences were 97%, while phylogenetic analysis showed that genotype of 2 viral belongs to the B.
CONCLUSIONSThere existed B Genotype of Aichi virus in China, and more research is needed to clarified the etiology and epidemiology of Aichi virus characteristics.
Child ; China ; Diarrhea ; virology ; Feces ; virology ; Humans ; Infant ; Kobuvirus ; classification ; genetics ; isolation & purification ; Molecular Sequence Data ; Phylogeny ; Picornaviridae Infections ; virology
3.Causative agents and epidemiology of diarrhea in Korean native calves
Sung Hwan LEE ; Ha Young KIM ; Eun Wha CHOI ; Doo KIM
Journal of Veterinary Science 2019;20(6):e64-
Calf diarrhea caused by infectious agents is associated with economic losses in the cattle industry. The purpose of this study was to identify the causative agents and epidemiological characteristics of diarrhea in Korean native calves (KNC). In total, 207 diarrheal KNC aged less than 7 months were investigated. Fecal samples collected from the rectum were examined for causative agents using polymerase chain reaction (PCR) or real-time PCR and the number of oocysts were counted. Fourteen causative agents were detected from 164 of the 207 diarrheal KNC. Rotavirus was the most common agent (34.8%), followed by Eimeria spp. (31.7%), Escherichia coli (22.0%), Giardia spp. (14.0%), Clostridium difficile (9.8%), bovine viral diarrhea virus (8.5%), coronavirus (7.9%), Cryptosporidium spp. (7.3%), torovirus (6.7%), parvovirus (5.5%), norovirus (4.9%), kobuvirus (1.8%), adenovirus (1.2%), and Salmonella spp. (0.6%). About 95 (57.9%) of 164 calves were infected with a single causative agent and 42.1% were infected by multiple agents. No significant difference was observed in mortality between calves infected with a single agent and multiple agents. The occurrence of diarrhea caused by rotavirus, Eimeria spp., kobuvirus, and Giardia spp. was significantly different based on onset age, and the prevalence of diarrhea caused by rotavirus or C. difficile was significantly different between seasons. This study help the understanding of KNC diarrhea for the development of an effective strategy for disease prevention and control, especially in Eastern provinces of South Korea.
Adenoviridae
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Age of Onset
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Animals
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Cattle
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Clostridium difficile
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Coronavirus
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Cryptosporidium
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Diarrhea
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Eimeria
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Epidemiology
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Escherichia coli
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Giardia
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Kobuvirus
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Korea
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Mortality
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Norovirus
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Oocysts
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Parvovirus
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Polymerase Chain Reaction
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Prevalence
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Real-Time Polymerase Chain Reaction
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Rectum
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Rotavirus
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Salmonella
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Seasons
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Torovirus