This paper is presented to report the results of urinary quantitative culture and sensitivity tests of certain antibiotics. Studies were done on 331 cases from the Department of Urology. Woo Sok University Medical College Hospital during the period from May 1962 up to September 1970. 1) Urine specimens from 331 cases were cultured and, of these, 115 cases grew micro-organism while the remaining 216 cases showed no growth. Among the 115 cases, 84 cases(73%) revealed significant bacteriuria with counts over 10s/ml. colonies in urine while 31 cases(27%) were below 10(5)/ml. 2) Only 74 cases(88. 1%) among the 84 cases with significant bacteriuria could the bacteria be identified after centrifuging the specimens and doing a smear and Gram stain. 68 cases(81%) of the total 84 significant bacteriuria cases had shown urinary leukocyte count of more than 4/HPF while 16 cases(19%) were below 4/HPF. 3) 77 cases(92%) of the total 84 cases with significant bacteriuria had Gram negative bacilli while 4 cases had Gram positive cocci by direct smear. 4) There were no significant differences between male and female patients in colony counts except the female group revealed some preponderance of E. coli. 5) In the sensitivity testing if the cultured significant bacteriuria in the group with Gram negative bacilli, 36 cases(49.3%) were sensitive to Kanamycin and 22 cases(30.1%) were resistant, while all cases were resistant to leucomycin and only one case was sensitive to penicillin among the total 73 cases of Gram negative bacilli cultured.
Anti-Bacterial Agents ; Bacteria ; Bacteriuria ; Female ; Gram-Positive Cocci ; Humans ; Kanamycin ; Kitasamycin ; Leukocyte Count ; Male ; Penicillins ; Urology

AIMTo identify the components of acetylleucomycin and its hydrolytic products by LC-MS.

METHODSAcetylleucomycin was separated on a Diamonsil C18 column with 0.1 mol x L(-1) ammonium acetate-acetontrile (35 : 65) as mobile phase. The LC-MS was equipped with an electorspray ion source (ESI), which was set at the positive ion mode, and the mass spectra of each component in chromatogram were obtained with difference cone voltage.

RESULTSThe components of acetylleucomycin and its hydrolytic products can be separated by HPLC. The components were identified according to the molecular weight and its major mass fragment ions. The major components identified in domastic acetylleucomycin were acetylleucomycin A4, A5; acetylleucomycin A1, A3; acetylleucomycin A6, A7, and acetylleucomycin A13. The hydrolytic products of acetylleucomycin were not kitasamycin, but some non-complete hydrolytic product.

CONCLUSIONThe method is rapid, sensitive and specific. It' s suitable to application in the fields of multi-components antibiotics analysis.

Chromatography, Liquid ; methods ; Hydrolysis ; Josamycin ; analysis ; chemistry ; Kitasamycin ; analogs & derivatives ; analysis ; chemistry ; Leucomycins ; analysis ; chemistry ; Macrolides ; analysis ; chemistry ; Spectrometry, Mass, Electrospray Ionization ; methods