Bacterial pneumonia and meningitis are major causes of childhood mortality in Papua New Guinea (PNG). Laboratory techniques for detection of bacterial pathogens have improved in the last decade, particularly molecular techniques that can be applied to culture-negative samples. With adequate training and support, a number of these techniques are readily available to research staff in PNG. In this article we summarize previous studies on the aetiology of pneumonia and meningitis in PNG, describe current diagnostic approaches and discuss available diagnostic tools to enhance surveillance of bacterial pneumonia and meningitis.