1.A study for acoustic parameters in unilateral vocal cord palsy.
Ki Hwan HONG ; Kil Yang CHUNG ; Mi Chung KIM ; Nam Kyun KIM ; Boo Kil KIM
Korean Journal of Otolaryngology - Head and Neck Surgery 1992;35(2):334-345
No abstract available.
Acoustics*
;
Vocal Cord Paralysis*
;
Vocal Cords*
2.A study of voice change after operation in unilateral vocal palsy.
Ki Hwan HONG ; Kil Yang CHUNG ; Mi Chung KIM ; Young Ki KIM
Korean Journal of Otolaryngology - Head and Neck Surgery 1992;35(6):761-769
No abstract available.
Paralysis*
;
Voice*
3.A Case of Mediastinal Gastroenteric Cyst.
Hong Ryang KIL ; Hye Suk HONG ; Yang Won LEE ; Jong Jin SEO ; Young hun CHUNG ; Seung Pyung LIM
Journal of the Korean Pediatric Society 1988;31(7):924-929
No abstract available.
4.Analysis of Glucocorticoid Response Element and TPA Response Element of Rat Thyrotropin-Releasing Hormine Gene by Site-Directed Mutagenesis.
Sung Woon KIM ; Jin Woo KIM ; Young Seol KIM ; Young Kil CHOI ; In Myoung YANG ; Jung Taek WOO ; Woon Won CHUNG
Journal of Korean Society of Endocrinology 1999;14(2):278-292
BACKGROUND: We previously demonstrated that a GRE/TRE composite sequence, which is located between 200 bp and 220 bp relative to the transcriptional start site of rat TRH gene, is responsible for the dexamethasone (DEX)- and TPA-induced transcriptional activation, and the transcriptional activation by DEX is mediated by interaction between glucocorticoid receptor (GR) and a TRE-binding transcriptional factor such as c-Jun. However, a non-specific binding with the transciption factors can not be excluded as the mutants used in the previous report could not inhibit the binding of GR and c-Jun completely, and it remains unclear which one of the two TRE-like sequences is critical for the interaction of the two transcription factors. METHODS: Luciferase expressing plasmids that contain a part of rat TRH promoter including the composite GRE sequence or its mutants were transfected into HeLa cells by Fugene 6. After the cells were incubated overnight with DEX or/and TPA, the luciferase activity was measured in a chemiluminometer. A gel retardation assay was performed after binding of the labeled composite sequence or its mutants with GR and c-Jun. RESULTS: DEX and TPA increased the transcriptional activity of the wild type composite sequence by 3 folds and 4 folds, respectively, and the combined stimulation increased the activity by 10 folds. The mutants of which all 6 nucleotides of the GRE half site were replaced and removed almost did not bind to GR and eould not enhance the transcriptional activity at all in response to DEX. The GRE-deleted mutant bound to c-Jun with a remarkably lower affinity and showed a lower response to TPA, whereas the GRE-replaced mutant bound to c-Jun with a similar affinity and showed a similar response to TPA compared to those of the wild type. In response to the combined simulation with DEX and TPA, the mutants showed 30-40% of the trancriptional activity of the wild type. Basal transcriptional activity of all the TRE mutants was significantly lower than that of the wild type. While they almost could not bind to c-Jun, their binding affinity to GR was comparable to that of the wild type. Whereas the DEX- and TPA-induced transcriptional activity of 5 TRE mutant was 10% and 15% of that of the wild type, it responded to those agents in a similar pattern as the wild type. The 3 TRE mutant and the mutant of both TRE sites did not respond to DEX and TPA. The GRE-deleted mutant hardly formed the DNA-protein complex as did the wild type, while the GRE -replaced mutant could form the complex in a less amount with nuclear extract of HeLa celL CONCLUSION: These results suggest that GRE/TRE composite sequence of rat TRH gene specifically binds to GR and c-Jun, providing a site for interaction between the two transcription factors, and that both TRE sites play an important role in basal transcription, and that the 3 TRE site is more critical in the interaction between GRE and TRE for DEX-induced transcriptional activation. (J Kor Endocrinol 14:278-292, 1999)
Animals
;
Dexamethasone
;
Electrophoretic Mobility Shift Assay
;
HeLa Cells
;
Humans
;
Luciferases
;
Mutagenesis, Site-Directed*
;
Nucleotides
;
Plasmids
;
Rats*
;
Receptors, Glucocorticoid
;
Response Elements*
;
Transcription Factors
;
Transcriptional Activation
5.The Characterization of Glucocoritcoid Response Element(GRE) on the Promoter of Thyrotropin-Releasing Hormone(TRH) Gene.
Sung Woon KIM ; Jin Woo KIM ; Young Seol KIM ; Young Kil CHOI ; In Myoung YANG ; Jung Taek WOO ; Woon Won CHUNG
Journal of Korean Society of Endocrinology 1999;14(2):265-277
BACKGROUND: We previously demonstrated that the promoter of rat TRH gene has GRE half site (TGTTCT) between -210 bp and -205 bp flanking with similar sequences of TPA response element (TRE), TAGTCA, at a distance of several base pairs from the GRE half site. It promps us to hypothesize that this composite GRE/TRE sequence can provide a site for interaction between glucocorticoid receptor (GR) and c-Jun. Thus, we investigated whether the composite sequence mediates transcriptional regulation induced by dexamethasone (DEX) and 12-O-tetradecanoyl phobol-13-acetate (TPA), and whether it binds GR and c-Jun. METHODS: A luciferase expressing plasmids that contain a part of rat TRH promoter including the composite sequence or their mutants were transfected into HeLa cells by Fugene 6. After the cells were incubated overnight with DEX and TPA, the luciferase activity was measured in a chemiluminometer. A gel retardation assay was performed after binding of the labeled composite sequence or its mutants with GR and c-Jun. RESULTS: DEX increased the transcriptional activity of the plasmid containing the wild type GRE by 2.5 folds, and TPA increased the transcriptional activity by 4 folds. The simultaneous stimulation with DEX and TPA synergistically increased the transcriptional activity by 10 folds. Two mutants whose GRE half sits were altered showed no responses to DEX, and suppressed the TPA-induced or both agents-induced transcriptional activity by 50%. Two mutants whose TRE-like sites were altered suppressed the DEX-induced transcriptional activity by 20%, TPA-induced trarptional activity by 25%, and both agents-induced transcriptional activity by 50%. Gel retardation assay showed that the composite sequence fonned a complex with GR and its mutants bound to GR with remarkably less affinity. c-Jun also bound to the composite sequence to form two cornplexes with less affinity compared to the AP-1 consensus sequence. The mutants of the TRE-like sequence bound to c-Jun with a significantly lower affinity compared to that of the wild type. Simulateous binding of the composite sequence with GR and c-Jun did not form any larger complex. The complex of GR and the composite sequence was much smaller than that formed by c-Jun, suggesting that GR binds to the composite sequence as a monomer. CONCLUSION: These results suggest that the composite sequence of GRE half site and TRE-like site on the promoter of rat TRH gene provides binding sites for GR and c-Jun, which mediate the interaction between two signal transduction pathways. (J Kor Soc Endocrinol 14:265-277, 1999)
4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
;
Animals
;
Base Pairing
;
Binding Sites
;
Consensus Sequence
;
Dexamethasone
;
Electrophoretic Mobility Shift Assay
;
HeLa Cells
;
Humans
;
Luciferases
;
Plasmids
;
Rats
;
Receptors, Glucocorticoid
;
Response Elements
;
Signal Transduction
;
Transcription Factor AP-1
6.Electromyographically Triggered Electrical Stimulation on Shoulder Subluxation in Hemiplegic Stroke Patients.
Chung Yong YANG ; Min Cheol JOO ; Eun Young KIL ; Heon Soo KIM ; Yong Il SHIN
Journal of the Korean Geriatrics Society 2006;10(1):36-42
BACKGROUND: The aim of this study is to evaluate the effect of electromyographically triggered electrical stimulation (ETES) on shoulder subluxation in stroke patients. METHOD: Twenty-three patients, who had hemiplegic shoulder subluxation within 3 months after stroke attack, were included. They were assigned randomly to either a control group (11 subjects) or a study group (12 subjects), and divided clinically to mild and severe subgroups by the degree in subluxation. Patients in both groups were received physical therapy and used an arm-sling. Subjects in the study group were taken ETES using biofeedback training on posterior deltoid and the supraspinatus muscle of hemiplegic subluxation side, daily 30 minutes a session, five days a week for 6 weeks. Electromyographic signals, which are derived from patient's voluntary muscle contraction, that exceeded a preset threshold trigger an immediate stimulation to force movement completion. The effect of ETES was assessed by the degree of subluxation using radiologic measurements at pre-treatment and 6 weeks post-treatment. RESULTS: In the control group, the degree of subluxation was increased after 6 weeks (p>0.05). In the study group, the subluxation was significantly reduced (p<0.05). The significant difference after treatment 6 weeks in mild and severe subgroups implied similar improvement between subgroups. CONCLUSION: The ETES may be the one of effective treatment methods for reducing the severity of hemiplegic shoulder subluxation in stroke patients.
Biofeedback, Psychology
;
Electric Stimulation*
;
Hemiplegia
;
Humans
;
Muscle, Skeletal
;
Shoulder*
;
Stroke*
7.Review of clinical characteristics of primary hyperparathyroidism.
Hyung Keun CHUNG ; Deog Yoon KIM ; Jeong Taek WOO ; Sang Woon KIM ; In Myung YANG ; Jin Woo KIM ; Young Seol KIM ; Kwang Won KIM ; Young Kil CHOI
Journal of Korean Society of Endocrinology 1992;7(3):234-242
No abstract available.
Hyperparathyroidism, Primary*
8.MR Imaging of Pituitary Abscess: Report of Two Cases.
Yul LEE ; Kil Woo LEE ; Ho Chul KIM ; Hae Jung PARK ; Ik YANG ; Soo Young CHUNG
Journal of the Korean Radiological Society 1997;36(4):587-590
Pituitary abscess is a rare condition of the pituitary gland. We report MR imaging findings in two cases of surgically-confirmed pituitary abscess occurring in women aged 39 and 28. In both a peripheral rim enhancing lesion, similar to abscesses in other areas of brain, was seen in the pituitary fossa.
Abscess*
;
Brain
;
Female
;
Humans
;
Magnetic Resonance Imaging*
;
Pituitary Gland
9.Effect of Ga2 gene mutation on the Expression of Thyrotropin-Releasing Hormone ( TRH ) Receptor Gene in GH3 Cells.
Seung Joon PARK ; In Myung YANG ; Jeong Hwa RYU ; Joo Ho CHUNG ; Jee Chang JUNG ; Kye Chang KO ; Young Seol KIM ; Young Kil CHOI
Journal of Korean Society of Endocrinology 1997;12(3):357-363
10.Self analysis of external quality control data of Kyungpook National University Hospital.
Jung Sun YANG ; Eun Sook CHUNG ; Kyung Eun SONG ; Jang Soo SUH ; Won Kil LEE ; In Sik LEE ; Yong Suk KIM ; Jay Sik KIM
Korean Journal of Clinical Pathology 1991;11(1):49-71
No abstract available.
Gyeongsangbuk-do*
;
Quality Control*