Dendritic cell (DC)s are protessional antigen presenting cells and they have been used for antitumor immunotherapy or cell vaccines. However therapy using DC is restricted because the number of DC available from tissue is very low. Flt3 ligand (FL) has been known as a hematopoietic growth factor that increases proliferation of hematopoietic stem cells and progenitor cells, and recently it showed inducibility of dendritic cell (DC)s and signiticant antitumor effects in vivo. Thus FL will be frequently used for DC induction and antitumor immunotherapy in future. Here we constructed FL plasmid and studied its in vivo effect. FL plasmids were made by cloning of partial FL cDNA into pcDNA3 plasmid, and gene expression and protein producibility of FL plasmid were confirmed in Renca cells transfected with FL plasmid. Mice were injected with FL plasmid (100ug/mouse) three times and 20 days later mouse spleens were harvested for staining and RT-PCR. There were lots of blastogenic cells in the spleen of mice treated with FL plasmid. FL plasmid also induced DEC205, IL-12 and GM-CSF gene expression in mouse splenocyte. All these data suggest FL plasmid may be used for induction of DC and antitumor therapy as DNA adjuvant.
Mice ; Animals

No abstract available.
Interleukin-2*

The primary goal in the treatment of the nail bed avulsion injury is complete regrowing of the nail plate without any deformity. There are many procedures for treatment of the nail bed avulsion injury as a split-thickness skin graft, full-thickness nail bed graft, reverse dermal graft or split-thickness nail bed graft. From February, 1984 to June, 1985 at In-Chon Gill Hospital, 13 cases of nail bed avulsion injury were treated with split-thickness nail bed grafts. The intact residual part of the injured nail bed or the toe nail bed was served as a donor site. The results were as followings; 1. The incidence of nail bed avulsion injury of all hand injuries was 9.7%. 2. The main cause of injury was the industrial accident and more prevalent in the left hand. 3. The good result was obtained in the split-thickness nail bed graft for the nail bed avulsion injury, but in nail root portion, the poor result was noticed. 4. The average time for normal nail appearance is six months.
Accidents, Occupational ; Animals ; Congenital Abnormalities ; Gills ; Hand ; Hand Injuries ; Humans ; Incheon ; Incidence ; Skin ; Tissue Donors ; Toes ; Transplants

No abstract available.
Cyclosporine* ; Dermatomyositis*

A case of a primary malignant melanoma of the right parietoccipital lobe is presented. The patient was a 49-year old man who had suffered headache, dizziness, recent memory disturbance & gait disturbance for 6 months. Total surgical removal of the lesion was performed without neurological deficit & the prognosis was good. Primary malignant melanoma of the brain are rarely reported in the literature. The possible etiology & management of the condition are discussed & literature is reviewed.
Brain ; Central Nervous System* ; Dizziness ; Gait ; Headache ; Humans ; Melanoma* ; Memory ; Middle Aged ; Prognosis

No abstract available.
Amlodipine* ; Humans ; Hypertension*

No abstract available.
Doxazosin* ; Hypertension*

No abstract available.
Humans ; Hypercholesterolemia* ; Lovastatin*

Intracranial tumor with extracranial metastasis has been seldom reported. A 28 year-old-male was admitted to Presbyterian Hospital in Daegu on May 9, 1977. Who was diagnosed to have a medulloblastoma in the right cerebellar hemisphere with metastases to the right axillary lymphnode and head of pancreas by pathological reports. In generally medulloblastoma with extracranial metastasis occurs very rarely in the cerebellar hemisphere, and especially in adulthood. Distant metastasis of the intracranial tumor is discussed with review of the literatures.
Daegu ; Head ; Medulloblastoma* ; Neoplasm Metastasis ; Pancreas ; Protestantism

Pituitary apoplexy is defined as a complex series of clinical events occurring as a consequence of the fulminant expansion of the pituitary tumor by infarction, hemorrhage, or hemorrhagic infarction of the tumor and the adjacent pituitary tissue. It is one of the entities which need neurosurgical emergency care. Its incidence was, however, reported variously in many literatures due to different diagnostic criteria. We reviewed 150 histologically proven pituitary adenoma cases, operated from January 1980 to September 1986, to select 30 cases which showed clinical or operative findings compatible with pitultary apoplexy('pituitary apoplexy in a broad sense'). Authors analyzed their incidence, presidposing factor, clinical feature, diagnosis and treatment, especially in those who showed major attack with evident enurologic deficits('pituitary apoplexy in a strict sense'). The conclusions are as follows; 1) Among the 150 cases of pituitary tumor, 30 cases (20.0%) were diagnosed as pituitary apoplexy in a broad sense. Eleven cases (7.3%) out of them showed major attack. 2) The symptoms and signs of the 11 cases with major attack were sudden headache(11 cases), visual impairment (9 cases), cavernous sinus compression signs (7 cases), nausea and vomiting (6 cases), depressed consciouness (4 cases), and meningismus (3 cases). 3) In the clinical diagnosis of pituitary apoplexy, findings of plain skull radiologic view were very helpful. 4) With the medical treatment and surgery, good results were achieved in all cases except one patient showing visual impairment. 5) As the method of surgical treatment, the transsphenoidal approach is highly recommended.
Cavernous Sinus ; Diagnosis* ; Emergency Medical Services ; Hemorrhage ; Humans ; Incidence ; Infarction ; Meningism ; Nausea ; Pituitary Apoplexy* ; Pituitary Neoplasms ; Skull ; Stroke ; Vision Disorders ; Vomiting