1.Fabry's disease: report of a case.
Dong-lan LUO ; Yan-hui LIU ; Ming-hui ZHANG ; Jun YAO
Chinese Journal of Pathology 2007;36(10):716-716
Adult
;
Fabry Disease
;
pathology
;
Female
;
Follow-Up Studies
;
Humans
;
Kidney Glomerulus
;
pathology
;
ultrastructure
;
Proteinuria
2.Rapid processing of samples for electron microscopy using paraffin-embedded renal biopsy tissues.
Chinese Journal of Pathology 2011;40(1):48-49
Basement Membrane
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pathology
;
ultrastructure
;
Biopsy
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Glomerulonephritis, IGA
;
pathology
;
Humans
;
Kidney
;
pathology
;
ultrastructure
;
Kidney Glomerulus
;
pathology
;
ultrastructure
;
Lupus Nephritis
;
pathology
;
Microscopy, Electron, Transmission
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Paraffin Embedding
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Specimen Handling
;
methods
3.Lipoprotein glomerulopathy: clinical features and pathological characteristics in Chinese.
Hui-ping CHEN ; Zhi-hong LIU ; Ru-jun GONG ; Zheng TANG ; Cai-hong ZENG ; Mao-yan ZHU ; Jian-ping WANG ; Hong ZHOU ; Lei-shi LI
Chinese Medical Journal 2004;117(10):1513-1517
BACKGROUNDLipoprotein glomerulopathy (LPG), once recognized as a rare glomerular disease, has been reported around the world in recent years. In this study, we reported 8 patients of LPG and aimed to explore the clinical features and pathological characteristics of LPG under light microscope, immunofluorescence staining, and electron microscope.
METHODSClinical manifestations were recorded on the day of renal biopsy. Biochemical patterns of lipids and lipoproteins were detected by routine examination. Plasma concentrations of apo B and apo E were determined by radial immunodiffusion assays. Biopsy specimens were then processed for light microscopy, immunohistochemical staining for immunoglobulins and complement components, and electron microscopy. Glomerular deposition of apo A, B, and E were detected using monoclonal antibodies on cryostatic sections.
RESULTSAll of the eight patients presented with edema, microscopic hematuria, severe proteinuria, anemia, and enlarged kidney size. Biochemical profiles revealed high levels of triglycerides, apo B, and apo E. We noted increments of glomerular size and lipoprotein thrombi occupying capillary lumina in the glomeruli of all patients. Immunofluorescence staining showed that the thrombi were strongly positive for apo A, B, and E. Granules and various sizes of vacuoles were observed in the thrombi under electron microscope.
CONCLUSIONCompared with previous reports on LPG in other countries, unique clinical and pathological features were found in this group of Chinese LPG patients.
Adolescent ; Adult ; Child ; Female ; Humans ; Kidney Diseases ; pathology ; Kidney Glomerulus ; pathology ; ultrastructure ; Lipoproteins ; metabolism ; Male ; Microscopy, Fluorescence ; Middle Aged
4.Fibronectin glomerulopathy: report of a case.
Xin ZHANG ; Su-xia WANG ; Qi-zhuang JIN ; Ming-hui ZHAO ; Wan-zhong ZOU
Chinese Journal of Pathology 2007;36(1):61-62
5.Clinicopathologic features of membranous nephropathy coexisting with IgA nephropathy.
Su-xia WANG ; Wan-zhong ZOU ; Li YANG ; Ming-hui ZHAO
Chinese Journal of Pathology 2007;36(3):171-174
OBJECTIVETo study the clinicopathologic features of membranous nephropathy coexisting with IgA nephropathy.
METHODSThe renal biopsies performed in Peking University First Hospital during the period from January, 1998 to April, 2006 were retrospectively reviewed. The clinicopathologic features of 11 cases of membranous nephropathy coexisting with IgA nephropathy were studied. Electron microscopy with immunogold labeling for IgG and IgA were also performed.
RESULTSThe mean age of patients was 39.9 years. The male-to-female ratio was 1:2.9. The patients mainly presented with proteinuria. Proteinuria of nephrotic level was seen in 7 cases (63.6%). Seven cases also had associated microscopic hematuria. None of them showed evidence of renal insufficiency. Cases with secondary diseases, such as hepatitis virus infection and systemic lupus erythematosus, were excluded from the study. Histologically, vacuolation and thickening of glomerular basement membrane was seen. There was also mild mesangial hypercellularity and increase in mesangial matrix. Occasional glomeruli with crescent formation were identified in 2 cases. Immunofluorescence study showed granular staining for IgG and C3 along glomerular capillary walls, in addition to clumps of IgA deposits in mesangium. Electron microscopy revealed subepithelial and mesangial electron-dense deposits. Immunogold labeling showed IgG and IgA localized in the subepithelial and mesangial deposits respectively.
CONCLUSIONMembranous nephropathy coexisting with IgA nephropathy possesses the clinicopathologic features of both components. It might be caused by independent occurrence of the two entities.
Adult ; Female ; Glomerular Basement Membrane ; immunology ; pathology ; ultrastructure ; Glomerular Mesangium ; immunology ; pathology ; ultrastructure ; Glomerulonephritis, IGA ; complications ; immunology ; pathology ; Glomerulonephritis, Membranous ; complications ; immunology ; pathology ; Humans ; Immunoglobulin A ; metabolism ; Immunoglobulin G ; metabolism ; Kidney Glomerulus ; immunology ; pathology ; ultrastructure ; Male ; Middle Aged ; Retrospective Studies
6.A Case of Light Chain Deposition Disease Involving Kidney and Bone Marrow with Microangiopathic Hemolytic Anemia.
Young Uk CHO ; Hyun Sook CHI ; Chan Jeoung PARK ; Seongsoo JANG ; Yong Mee CHO ; Jung Sik PARK
The Korean Journal of Laboratory Medicine 2009;29(5):384-389
We report a case of light chain deposition disease in a 59-yr-old female showing deposition of monoclonal light chain in the kidney and bone marrow accompanied with a schistocytosis, the morphologic finding of microangiopathic hemolytic anemia. The immunofluorescence examination of the kidney revealed strongly stained kappa-light chain deposits on the glomerular mesangium and capillary wall, tubules, and vessel wall. The electron microscopy demonstrated electron-dense deposits on the glomerular basement membrane and mesangium. Anemia was observed with schistocytosis and Howell-Jolly body in the peripheral blood smears. The immunohistochemical examination of the bone marrow showed the presence of kappa-light chain deposits in scattered plasma cells and thickened vessel wall in the absence of a prominent plasma cell proliferation. Although an immunofixation electrophoresis failed to detect a monoclonal gammopathy, the presence of monoclonal protein could be identified by an abnormal kappa/lambda ratio on the serum free light chain analysis.
Anemia, Hemolytic/complications/*diagnosis
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Bone Marrow/*pathology
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Female
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Glomerulonephritis/complications/*diagnosis/pathology
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Humans
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Immunoglobulin Light Chains/*analysis
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Kidney Glomerulus/*pathology/ultrastructure
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Middle Aged
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Paraproteinemias/complications/*diagnosis/immunology
7.Pathological features of light chain nephropathy.
Su-xia WANG ; Wan-zhong ZOU ; Ye ZHANG ; Shu-he WANG ; Li-jun CHAI ; Xiu-ying TANG
Chinese Journal of Pathology 2003;32(6):506-510
OBJECTIVETo investigate the pathologic features and diagnostic algorithm of light chain nephropathy (LCN).
METHODSSeven cases of LCN were studied by light microscopy, electron microscopy and immunolabeling of light chains (kappa, lambda) by immunofluorescence and immunoelectron microscopy.
RESULTSThe histopathology of 7 cases by light microscopy was variable, with 3 cases showing nodular glomerulosclerosis, 1 case showing mild to moderate mesangial proliferation, and 3 cases showing cast nephropathy with minimal glomerular change. Immunofluorescence study revealed positive staining of a single type of light chain in mesangium (nodular pattern) or along glomerular basement membrane (linear), along tubular basement membrane and around arteriolar walls in all the 7 cases. Ultrastructurally, electron-dense granular deposits were identified in mesangium, subendothelial aspect of glomerular basement membrane, outer aspect of tubular basement membrane and arteriolar walls. Immunogold labeling of light chains showed distinct labeling of a single type light chain in the granular electron-dense materials (5 cases being kappa-positive and 2 being lambda-positive).
CONCLUSIONSLCN typically shows nodular glomerulosclerosis. The ultrastructural change is characteristic and important for diagnosis. Immunolabeling of light chains by immunofluorescence and immunoelectron microscopy carries further diagnostic value, especially in cases with minimal light microscopic change.
Adult ; Aged ; Female ; Glomerulosclerosis, Focal Segmental ; immunology ; pathology ; Humans ; Immunoglobulin Light Chains ; immunology ; Immunoglobulin kappa-Chains ; immunology ; Immunoglobulin lambda-Chains ; immunology ; Kidney Diseases ; immunology ; pathology ; Kidney Glomerulus ; immunology ; pathology ; ultrastructure ; Male ; Microscopy, Fluorescence ; Microscopy, Immunoelectron ; Middle Aged
8.Effect of reactive oxygen species and transforming growth factor-beta1 on progressive passive Heymann's nephritis.
Hong DENG ; Zhao-li LI ; Dong-sheng ZHANG ; Xiang FAN ; Dong-feng LIU ; Ning SU
Chinese Journal of Pathology 2005;34(3):175-176
Animals
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Collagen Type IV
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metabolism
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Fibronectins
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metabolism
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Glomerulonephritis, Membranous
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metabolism
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pathology
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Immune Sera
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immunology
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Kidney Glomerulus
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pathology
;
ultrastructure
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Male
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Malondialdehyde
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blood
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Rats
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Rats, Sprague-Dawley
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Reactive Oxygen Species
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pharmacology
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Superoxide Dismutase
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blood
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Taurine
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pharmacology
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Transforming Growth Factor beta1
;
metabolism
9.Huaiqihuang Granules () reduce proteinuria by enhancing nephrin expression and regulating necrosis factor κB signaling pathway in adriamycin-induced nephropathy.
Hong LIU ; Wei SUN ; Liu-Bao GU ; Yue TU ; Bing-Yin YU ; Hao HU
Chinese journal of integrative medicine 2017;23(4):279-287
OBJECTIVETo investigate the effects of Huaiqihuang Granules (, HQH), a mixture of Chinese herbs including Trametes robiniophila Murr, Fructus Lycii and Polygonatum sibiricum, on adriamycininduced nephropathy (ADRN) in rats and its underlying mechanisms.
METHODSRats with ADRN were divided into four groups: the sham group, the model group (distilled water), the low-dose HQH-treated (2 g/kg) group, and the high-dose HQH-treated (4 g/kg) group. Body weight and 24-h urinary protein (Upro) were checked every week. After 5-week intervention, at the end of the study, the rats were sacrificed and blood samples were collected for examination of biochemical parameters, including glomerular morphological makers, podocyte shape, cellular apoptosis, expressions of nephrin, inflammatory and apoptosis markers.
RESULTSHQH ameliorated the rat's general status, proteinuria, renal morphological appearance and glomerulosclerosis. The decreased expression of nephrin in ADRN rats was increased by HQH, as well as the impaired podocyte foot process fusion. Cytosolic levels of p65 and inhibitor of nuclear factor κBα (IκBα) were decreased in ADRN rats, and recovered by the treatment of HQH. Consistently, the induced expression of tumor necrosis factor α (TNF-α), phosphorylated nuclear factor κB p65 (p-NFκB p65) and IκBα in ADRN were markedly suppressed by HQH. In addition, induction of Bax, cleaved caspase-3 and cytochrome C in ADRN rats were suppressed by HQH, indicating the amelioration of apoptosis.
CONCLUSIONHQH could ameliorate renal impairments in ADRN rats by increasing nephrin expression, inhibiting NF-κB signaling pathway via the down-regulation of p-NF-κB p65 and p-IκBα, and suppression of glomerular and tubular apoptosis.
Animals ; Apoptosis ; drug effects ; Body Weight ; drug effects ; Caspase 3 ; metabolism ; Chromatography, High Pressure Liquid ; Cytochromes c ; metabolism ; Doxorubicin ; adverse effects ; Drugs, Chinese Herbal ; pharmacology ; therapeutic use ; Kidney ; drug effects ; pathology ; Kidney Diseases ; blood ; chemically induced ; complications ; drug therapy ; Kidney Glomerulus ; drug effects ; pathology ; ultrastructure ; Kidney Tubules ; drug effects ; pathology ; ultrastructure ; Male ; Membrane Proteins ; metabolism ; NF-KappaB Inhibitor alpha ; metabolism ; NF-kappa B ; metabolism ; Organ Size ; drug effects ; Proteinuria ; blood ; complications ; drug therapy ; Rats, Sprague-Dawley ; Signal Transduction ; drug effects ; Transcription Factor RelA ; metabolism ; Tumor Necrosis Factor-alpha ; metabolism ; bcl-2-Associated X Protein ; metabolism