1.Clinicopathologic features of membranous nephropathy coexisting with IgA nephropathy.
Su-xia WANG ; Wan-zhong ZOU ; Li YANG ; Ming-hui ZHAO
Chinese Journal of Pathology 2007;36(3):171-174
OBJECTIVETo study the clinicopathologic features of membranous nephropathy coexisting with IgA nephropathy.
METHODSThe renal biopsies performed in Peking University First Hospital during the period from January, 1998 to April, 2006 were retrospectively reviewed. The clinicopathologic features of 11 cases of membranous nephropathy coexisting with IgA nephropathy were studied. Electron microscopy with immunogold labeling for IgG and IgA were also performed.
RESULTSThe mean age of patients was 39.9 years. The male-to-female ratio was 1:2.9. The patients mainly presented with proteinuria. Proteinuria of nephrotic level was seen in 7 cases (63.6%). Seven cases also had associated microscopic hematuria. None of them showed evidence of renal insufficiency. Cases with secondary diseases, such as hepatitis virus infection and systemic lupus erythematosus, were excluded from the study. Histologically, vacuolation and thickening of glomerular basement membrane was seen. There was also mild mesangial hypercellularity and increase in mesangial matrix. Occasional glomeruli with crescent formation were identified in 2 cases. Immunofluorescence study showed granular staining for IgG and C3 along glomerular capillary walls, in addition to clumps of IgA deposits in mesangium. Electron microscopy revealed subepithelial and mesangial electron-dense deposits. Immunogold labeling showed IgG and IgA localized in the subepithelial and mesangial deposits respectively.
CONCLUSIONMembranous nephropathy coexisting with IgA nephropathy possesses the clinicopathologic features of both components. It might be caused by independent occurrence of the two entities.
Adult ; Female ; Glomerular Basement Membrane ; immunology ; pathology ; ultrastructure ; Glomerular Mesangium ; immunology ; pathology ; ultrastructure ; Glomerulonephritis, IGA ; complications ; immunology ; pathology ; Glomerulonephritis, Membranous ; complications ; immunology ; pathology ; Humans ; Immunoglobulin A ; metabolism ; Immunoglobulin G ; metabolism ; Kidney Glomerulus ; immunology ; pathology ; ultrastructure ; Male ; Middle Aged ; Retrospective Studies
2.Clinicopathologic features of collagen III glomerulopathy.
Haijing LIU ; Jian CHEN ; Yan ZHANG ; Shenglan WANG ; Wanzhong ZOU
Chinese Journal of Pathology 2014;43(11):732-735
OBJECTIVETo study the clinicopathologic features of collagen III glomerulopathy and its cause, pathogenesis and prognosis.
METHODSFive cases of collagen III glomerulopathy that collected from 2005 to 2014 were observed by renal biopsy. The morphologic characteristics were studied by light microscopy, immunofluorescence, immunohistochemical and electron microscopy.
RESULTSThe glomerular mesangium became expansion but no hypercellularity, basement membrane appeared thickened. The glomeruli showed collagen type III deposit by immunohistochemistry method, and collagen fibers increased by electron microscopy. The patients often show serious proteinuria, nephrotic syndrome and renal function damage.
CONCLUSIONSCollagen III glomerulopathy is an idiopathic glomerular disease, characterized by massive accumulation of collagen type III within the glomerular mesangial areas and basement membrane. Collagen III glomerulopathy is extremely rare. The etiology and pathogenesis may relate to the abnormality of collagen III gene. There is no specific treatment for it and its prognosis is poor.
Basement Membrane ; metabolism ; Biopsy ; Collagen Type III ; genetics ; metabolism ; Female ; Fluorescent Antibody Technique ; Glomerular Mesangium ; metabolism ; Humans ; Immunohistochemistry ; Kidney Diseases ; etiology ; pathology ; Kidney Glomerulus ; pathology ; Microscopy, Electron ; Prognosis ; Proteinuria ; diagnosis
3.Two cases of Type Ⅲ collagen glomerulopathy and literature review.
Fang YU ; Xuejing ZHU ; Shuguang YUAN ; Zailiang GONG ; Xiangqing XU ; Hong LIU ; Jun LI ; Lin SUN ; Fuyou LIU
Journal of Central South University(Medical Sciences) 2020;45(7):869-873
In this paper, 2 cases of collagen Type Ⅲ glomerulopathy were analyzed. The clinical manifestations mainly included nephrotic syndrome, proteinuria, hypertension and renal dysfunction. One patient showed that the complement factor H-related protein 5 (CFHR5) gene was likely a disease-causing mutation. The pathological examination of renal tissues showed hyperplasia of mesangial matrix, sub-endothelial insertion, and double-track formation. Immunohistochemistry of Type III collagen was positive. Electron microscopy revealed that massive collagen fibers (40-70 nm in diameter) deposited in the mesangial matrix and basement membrane. As for the follow-up results, the normal renal function had kept steady and the proteinuria was moderate in 1 case treated with angiotensin Ⅱ receptor blocker. Due to other system disease, another case developed into acute kidney injury and then received hemodialysis. The clinical manifestations of collagen Type Ⅲ glomerulopathy was atypical, the light microscope pathological features were various, and the disease was mainly diagnosed by electron microscopy and immunohistochemistry.
Collagen Type III
;
genetics
;
Glomerular Mesangium
;
Humans
;
Kidney Diseases
;
Kidney Glomerulus
;
Proteinuria
4.Spatial relationship between expression of cytokeratin-19 and that of connexin-43 in human fetal kidney.
Keisuke HIEDA ; Shogo HAYASHI ; Ji Hyun KIM ; Gen MURAKAMI ; Baik Hwan CHO ; Akio MATSUBARA
Anatomy & Cell Biology 2013;46(1):32-38
Connexin-43, a major gap junction protein, and cytokeratin-19, one of the intermediate filament keratins, are known to be markers of well-differentiated epithelium. In this study, we investigated the expression of these markers in the head region, lungs, and abdominal organs of 10 human mid-term fetuses. The expression of connexin-43 was found to be restricted to the dura mater, kidney, and adrenal cortex. In the kidney, we found a clear site-dependent difference in the expression pattern of these markers: connexin-43 expression was observed in the tubules of the renal cortex whereas cytokeratin-19 was strongly expressed in the collecting ducts and renal pelvis. This difference remained unchanged throughout the fetal stages examined. Immunoreactivity was not observed for either of the markers in the intrarenal vessels, including the glomeruli, and mesangial cells. Connexin-43 expression seemed to be restricted to the metanephric vesicle-derived structures that differentiate in the urogenital ridge of the splanchnic mesoderm. The adrenal cortex also originates from the same para-aortic mesoderm. In contrast, in the urogenital organs, cytokeratin-19 seemed to be expressed in ducts derived from the urogenital sinus.
Adrenal Cortex
;
Connexins
;
Dura Mater
;
Epithelium
;
Fetus
;
Head
;
Humans
;
Immunohistochemistry
;
Intermediate Filaments
;
Keratin-19
;
Keratins
;
Kidney
;
Kidney Pelvis
;
Lung
;
Mesangial Cells
;
Mesoderm
5.Effect of Tujian decoction on protein kinase C activity in renal cortex in diabetic rat.
Yuan-lin PIAO ; Xiao-chun LIANG ; Nan YANG ; De-hai YIN ; Ping-ping ZUO
China Journal of Chinese Materia Medica 2004;29(7):685-689
OBJECTIVETo investigate influence of administration of Tujian decoction (Chinese herbal medicine) on protein kinase C (PKC) activity, renal function and structure in diabetic rat kidney.
METHODExperimental diabetic nephropathy model was induced by nephrectomy combined with streptozotocin (STZ) injection in sprague-dawley rat. Tujian decoction (20 g x kg(-1) x d(-1)) and Valsartan (20 mg x kg(-1) x d(-1)) were orally administrated respectively for 12 weeks. PKC activity was measured by [3H]phorbol 12, 13-dibutyrate ([3H]PDBu) binding assay. 24 h urine protein excretion (Upro) and renal pathological changes were observed.
RESULTIn 12th week, diabetic nephrectomized rats developed proteinuria, glomerulosclerosis, increased membrane PKC activity (mPKC), decreased cytosol PKC (cPKC), and increased ratio of mPKC and cPKC (M/C). Administration of Tujian decoction or Valsartan led to a reduction in proteinuria, structural injury, mPKC and M/C, and a recovery in cPKC.
CONCLUSIONTujian decoction possesses a renoprotective effect on diabetic nephrectomized rat, at least partially via the inhibition of PKC activation in renal cortex.
Animals ; Cuscuta ; chemistry ; Diabetes Mellitus, Experimental ; enzymology ; pathology ; Drug Combinations ; Drugs, Chinese Herbal ; isolation & purification ; pharmacology ; Euonymus ; chemistry ; Kidney Cortex ; enzymology ; Kidney Glomerulus ; pathology ; Male ; Plants, Medicinal ; chemistry ; Protective Agents ; pharmacology ; Protein Kinase C ; metabolism ; Rats ; Rats, Sprague-Dawley
6.A Case of Membranoproliferative Glomerulonephritis Type II (Dense-Deposit Disease).
Suk Jin LEE ; Jae Hoon MOON ; Mi Seon KANG ; Min Seob SONG ; Woo Yeong CHUNG
Journal of the Korean Society of Pediatric Nephrology 2003;7(2):204-210
Membranoproliferative glomerulonephritis type II(MPGN II), also called dense deposit disease, was first described by Berger and Galle in 1963. The diagnosis of MPGN II is based on electron-microscopic finding of an intensely electron-dense substance which replaces the lamina densa of the glomerular basement membrane. Although the etiology and pathogenesis of MPGN II are unknown, it frequently progresses to end-stage renal failure. Typically in MPGN II, hypocomplementemia due to activation of the alternative complement pathway is present. In addition, the association of MPGN II with partial lipodystrophy and complement abnormalities is well documented. The relationship between these associated features and the patient's renal functional outcome is not clear. With respect to the therapy for MPGN II, an alternate-day prednisolone regimen was shown to be effective. Various treatment modalities, including immunosuppression with corticosteroids, cytotoxic drugs and cyclosporin A, anticoagulants and antiplatelet therapies are used, either alone or in combination, with varying degrees of success. The purpose of this paper is to present a case of MPGN II from a 7 years old girl with paroxysmal supraventricular tachycardia(PSVT).
Adrenal Cortex Hormones
;
Anticoagulants
;
Child
;
Complement Pathway, Alternative
;
Complement System Proteins
;
Cyclosporine
;
Diagnosis
;
Female
;
Glomerular Basement Membrane
;
Glomerulonephritis, Membranoproliferative*
;
Humans
;
Immunosuppression
;
Kidney Failure, Chronic
;
Lipodystrophy
;
Prednisolone
7.A Case of Membranoproliferative Glomerulonephritis Type II (Dense-Deposit Disease).
Suk Jin LEE ; Jae Hoon MOON ; Mi Seon KANG ; Min Seob SONG ; Woo Yeong CHUNG
Journal of the Korean Society of Pediatric Nephrology 2003;7(2):204-210
Membranoproliferative glomerulonephritis type II(MPGN II), also called dense deposit disease, was first described by Berger and Galle in 1963. The diagnosis of MPGN II is based on electron-microscopic finding of an intensely electron-dense substance which replaces the lamina densa of the glomerular basement membrane. Although the etiology and pathogenesis of MPGN II are unknown, it frequently progresses to end-stage renal failure. Typically in MPGN II, hypocomplementemia due to activation of the alternative complement pathway is present. In addition, the association of MPGN II with partial lipodystrophy and complement abnormalities is well documented. The relationship between these associated features and the patient's renal functional outcome is not clear. With respect to the therapy for MPGN II, an alternate-day prednisolone regimen was shown to be effective. Various treatment modalities, including immunosuppression with corticosteroids, cytotoxic drugs and cyclosporin A, anticoagulants and antiplatelet therapies are used, either alone or in combination, with varying degrees of success. The purpose of this paper is to present a case of MPGN II from a 7 years old girl with paroxysmal supraventricular tachycardia(PSVT).
Adrenal Cortex Hormones
;
Anticoagulants
;
Child
;
Complement Pathway, Alternative
;
Complement System Proteins
;
Cyclosporine
;
Diagnosis
;
Female
;
Glomerular Basement Membrane
;
Glomerulonephritis, Membranoproliferative*
;
Humans
;
Immunosuppression
;
Kidney Failure, Chronic
;
Lipodystrophy
;
Prednisolone
8.Relationship between podocyte injury and macrophage infiltration in renal tissues of patients with lupus nephritis.
Ying WANG ; Ming Hui LI ; Yan ZHANG ; Xiao Yan HU ; Rui Xia MA
Journal of Peking University(Health Sciences) 2019;51(4):723-727
OBJECTIVE:
To investigate the relationship between the expression of nephrin and the infiltration of macrophages in renal tissues in patients with lupus nephritis (LN), and to provide the evidence of potential mechanism of podocyte injury in LN.
METHODS:
In the study, 60 patients who were first diagnosed with LN by pathology were selected including 38 active LN patients with r-SLEDAI≥4, and another 10 patients of normal renal tissue were excised as a normal control group. The renal tissue and podocyte injury were observed through light and transmission electron microscope. The expression of nephrin and the infiltration of macrophages (CD68+cells) in the renal tissue of the 60 LN patients and 10 normal cases were detected by immunohistochemical and immunofluorescence method. Different statistical analysis methods were used to analyze the correlation between the variables. Variance analysis was used for comparison among the groups, while LSD test was used for comparison between every two groups. Pearson correlation analysis was used to analyze the correlation between the variables.
RESULTS:
(1)Of all the LN patients, 24 h urinary protein [(3.94±1.76) vs. (1.56±0.68), P<0.05], erythrocyte sedimentation rate (ESR) [(79.83±6.3) vs. (40.1±10.5), P<0.05] and serum creatinine [(106.58±14.9) vs. (79.1±9.89), P<0.05] were significantly increased in active group than those in inactive group, while C3 [(0.34±0.12) vs. (0.78±0.11), P<0.05], C4 [(0.07±0.04) vs. (0.17±0.10), P<0.05 ] and eGFR [(62.42±5.16) vs. (81.33±4.53), P<0.05] were significantly decreased in active group. (2)Compared with the normal control group, the expression of nephrin in renal tissue of the LN patients was significantly decreased, and the expression of nephrin in the active patients was significantly lower than that in inactive group (P<0.05). (3)Compared with the normal control group, the number of infiltrated macrophages in the LN patients was significantly increased, especially in the active patients (P<0.05). Macrophages were mainly found in glomeruli. (4)There was a significant negative correlation between the expression of nephrin and macrophage infiltration in renal tissues of the LN patients (r=0.761, P<0.001).
CONCLUSION
Macrophage infiltration in renal tissues may be one of the potential mechanisms of podocyte injury in lupus nephritis.
Humans
;
Kidney
;
Kidney Glomerulus
;
Lupus Nephritis
;
Macrophages
;
Podocytes
9.Transforming growth factor-beta and the glomerular filtration barrier.
Ayesha GHAYUR ; Peter J MARGETTS
Kidney Research and Clinical Practice 2013;32(1):3-10
The increasing burden of chronic kidney disease worldwide and recent advancements in the understanding of pathologic events leading to kidney injury have opened up new potential avenues for therapies to further diminish progression of kidney disease by targeting the glomerular filtration barrier and reducing proteinuria. The glomerular filtration barrier is affected by many different metabolic and immune-mediated injuries. Glomerular endothelial cells, the glomerular basement membrane, and podocytes-the three components of the filtration barrier-work together to prevent the loss of protein and at the same time allow passage of water and smaller molecules. Damage to any of the components of the filtration barrier can initiate proteinuria and renal fibrosis. Transforming growth factor-beta (TGF-beta) is a pleiotropic cytokine strongly associated with the fibrogenic response.It has a known role in tubulointerstitial fibrosis. In this review we will highlight what is known about TGF-beta and how it interacts with the components of glomerular filtration barrier and causes loss of function and proteinuria.
Endothelial Cells
;
Fibrosis
;
Filtration
;
Glomerular Basement Membrane
;
Glomerular Filtration Barrier
;
Kidney
;
Kidney Diseases
;
Podocytes
;
Proteinuria
;
Renal Insufficiency, Chronic
;
Transforming Growth Factor beta
;
Water
10.Transforming growth factor-beta and the glomerular filtration barrier.
Ayesha GHAYUR ; Peter J MARGETTS
Kidney Research and Clinical Practice 2013;32(1):3-10
The increasing burden of chronic kidney disease worldwide and recent advancements in the understanding of pathologic events leading to kidney injury have opened up new potential avenues for therapies to further diminish progression of kidney disease by targeting the glomerular filtration barrier and reducing proteinuria. The glomerular filtration barrier is affected by many different metabolic and immune-mediated injuries. Glomerular endothelial cells, the glomerular basement membrane, and podocytes-the three components of the filtration barrier-work together to prevent the loss of protein and at the same time allow passage of water and smaller molecules. Damage to any of the components of the filtration barrier can initiate proteinuria and renal fibrosis. Transforming growth factor-beta (TGF-beta) is a pleiotropic cytokine strongly associated with the fibrogenic response.It has a known role in tubulointerstitial fibrosis. In this review we will highlight what is known about TGF-beta and how it interacts with the components of glomerular filtration barrier and causes loss of function and proteinuria.
Endothelial Cells
;
Fibrosis
;
Filtration
;
Glomerular Basement Membrane
;
Glomerular Filtration Barrier
;
Kidney
;
Kidney Diseases
;
Podocytes
;
Proteinuria
;
Renal Insufficiency, Chronic
;
Transforming Growth Factor beta
;
Water