No abstract available.
Adhesives*

No abstract available.
Pyloric Stenosis, Hypertrophic*

BACKGROUND: Propioriacterium acnes plays an importantol in the development of inflammatory acne, and inflarnmatory lesions are improved by oralnc topical antibiotics. But as Pacnes frequently develop resistance to antibiotics in patients neing long term systemic antibiotic therapy, the theravuti effects diminish, and eventually thay fails. OBJECTIVE: The purpose of this study is to evaluate theerral susceptability of P. acnes to antibiotics and the difference in the MIC depending on the of oral and/or topical antibiotics, therapeutic effects and disease duration in patients with acie ulgaris. METHODS: We used twenty six strains of P. acnes which were obtained from patients with acne and performcd suseptibility testing for antibiotics usir the E test procedure. RESULTS: 1. The growth of P. acnes was completely inhibited by e ythromycin and chloramphenicol at concentrations of 0.023ug/ml and 0.064ug/ml, respectively cefoxitin at 0.094ug/ml, and by tetracycline and clindarnycin at 0.190 ug/ml. 2. P. acnes was mot susceptible to erythromycin, and olwed by chloramphenicol, cefoxitin, tetracycline, clindamycin in order of decreasing susceptibilit . 3. There were no significant differences in the MIC in reat in to previous antibiotic treatment. 4. For tetracycline, The MIC was significantly lower(p<0.01) in patients who improved after treatment. 5, For tetracycline and chloramphenicol, the MIC was grficantly lower(p<0.05) in patients with less than 2 years disease duration. CONCLUSION: The susptibility of antibiotics for P. acneias highest in erythromycin. There were no significant differences in the MIC in relation to prvious antibiotic treatment, and for some antibiotics the suseptibility was low in patients who dill not show clinical improvement or who had long disease duration.
Acne Vulgaris* ; Anti-Bacterial Agents* ; Asian Continental Ancestry Group ; Cefoxitin ; Chloramphenicol ; Clindamycin ; Erythromycin ; Humans ; Propionibacterium acnes* ; Propionibacterium* ; Tetracycline

BACKGROUND: Tandem duplication of chromosome 17p11.2-p12 including peripheral myelin protein 22 (PMP22) gene is the most frequent cause of Charcot-Marie-Tooth 1A (CMT1A). Patients carrying one extra copy of PMP22 develop CMT1A, whereas the deletion of the 17p11.2-p12 region causes hereditary neuropathy with the liability to pressure palsies (HNPP). In the present study, we established the genotyping methods of 6 microsatellite markers (D17S921, D17S9B, D17S9A, D17S4A, D17S918 and D17S122) within the 17p11.2-p12 regions by the hexaplex PCR for the genetic diagnosis of CMT1A duplication and HNPP deletion. METHODS: We established polymorphic behavior and genotyping methods of 6 microsatellite markers (D17S921, D17S9B, D17S9A, D17S4A, D17S918 and D17S122) within the duplication region. The 6 markers were amplified by hexaplex PCR reaction and analyzed by an automatic sequencing analyzer and genotyper program. RESULTS: The genotype distributions of all markers were not significantly deviated from the Hardy-Weinberg equilibrium (P>or=0.05). When comparing the control group and CMT1A, HNPP patients group by the distribution of allele, there is no significant difference in the 5 locus except in the 1 locus (D17S921) among HNPP patients. The specificity was more than 99.9%. The sensitivity of each CMT1 and HNPP was 56.3% (40/71 pedigrees) and 72.1% (31/43 HNPP pedigrees), respectively. CONCLUSIONS: The error rate for the system may be less than 0.001. According to this study, it is possible to have rapid and exact genetic diagnosis of both CMT1A and HNPP, which may be helpful for the development of personalized therapy according to genetic defects.
Alleles ; Charcot-Marie-Tooth Disease ; Diagnosis* ; Genotype ; Humans ; Microsatellite Repeats* ; Multiplex Polymerase Chain Reaction* ; Myelin Sheath ; Paralysis ; Polymerase Chain Reaction ; Sensitivity and Specificity

BACKGROUND: Methicillin-resistant Staphylococcus aureus (MRSA) continues to be a major cause of nosocomial infection and a molecular typing is necessary for proper epidemiologic investigations of sources and moles of spread in an outbreak. An nosocomial outbreak of MRSA in a neonatal intensive care unit at Ewha Womans University Mokdong Hospital was suspected. To investigate the clonality of isolates and control the spread of nosocomial outbreak, we performed plasmid restriction analysis of MRSA isolates from patients and medical staffs. METHODS: We studied 7 MRSA strains (umbilicus 4, blood 1, urine 1 and pus 1) from patients in a neonatal intensive care unit and the MRSA strains from nares and hands surveillance cultures of 26 medical staffs (4 medical doctors and 22 nurses). All MRSA strains were tested for antimicrobial susceptibility and plasmic analysis after EcoRI restriction. We analyzed the plasmid patterns of MRSA isolated from patients and compared with those from medical staffs. RESULTS: Ten MRSA strains (from 7 nares and 3 hands) were isolated from surveillance cultures of 26 medical staffs. Seven out of 10 MRSA strains from medical staffs revealed identical pattern of antibiogram which was the same pattern in all 7 MRSA strains from seven patients. Plasmid restriction patterns were classified 6 groups from A to F showing 2-10 bands. Six out of 7 MRSA strains from the patients showed group A(A1 5, A31) and 5 out of 10 MRSA strains from the medical staffs showed group A(A1 1, A21, A32, A41) and remainders showed different plasmid restriction analysis patterns. CONCLUSIONS: These results suggest that plasmid restriction analysis is a rapid, inexpensive, and good discriminating molecular typing of MRSA outbreak and is useful for the epidemiologic investigation of MRSA outbreaks in the clinical laboratory.
Cross Infection ; Disease Outbreaks ; Female ; Hand ; Humans ; Infant, Newborn ; Intensive Care, Neonatal ; Medical Staff ; Methicillin Resistance* ; Methicillin-Resistant Staphylococcus aureus* ; Microbial Sensitivity Tests ; Molecular Epidemiology* ; Molecular Typing ; Plasmids* ; Suppuration

No abstract available.
Colon, Descending* ; Leiomyosarcoma*

PURPOSE: This study was conducted to examine how nurses' death anxiety and terminal care stress affect their terminal care performance in the clinical setting. METHODS: The study enrolled 180 registered nurses with experience of attending dying patients at a university hospital located in Seoul, Korea. Collected data were analyzed with descriptive statistics, one-way ANOVA and Pearson's correlation using SPSS 18.0 for Windows. RESULTS: Nurses showed significant differences in the level of death anxiety and terminal care stress as well as terminal care performance by working division, marital status, educational background and hospice training. A significant relationship was found between terminal care stress and terminal care performance. CONCLUSION: The study results showed that efforts to ease nurses' death anxiety and terminal care stress could improve their terminal care performance. Further study should be conducted to investigate other factors that affect nurses' terminal care performance from various perspectives and develop a terminal care manual which can be used as guidance for nurses in charge of terminal patient care.
Anxiety ; Fees and Charges ; Hospices ; Humans ; Korea ; Marital Status ; Nursing Services ; Patient Care ; Terminal Care

Of the many potential source of free radial generation, the enzyme xanthine oxidase has been shown to be important in ischemia in non-cerebral tissues. Oxygen-derived free radicals have been implicated in various pathological conditions including ischemia. Xanthine oxidase serve as a source of oxidizing agents such as superoxide radical and hydrogen peroxide. It is investigated that the effect of a specific xanthine oxidase inhibitor, allopurinol, on infarct size in a model of continuous partial cerebral ischemia in rats. Infarct volume was measured by triphenyl tetrazolium chloride staining of brains removed 24 hours after middle cerebral arterial occlusion. Cortical tissue was more effectively protected than basal tissue, especially in allopurinol pretreated group. On histological examinations, hemispheric swelling, PMN cell infiltration and endothelial damage were noted irrespective of allopurinol treatment. It was speculated that free radicals are important in infarction secondary to partial continuous cerebral ischemia and that xanthine oxidase may by the primary source of these radicals.
Allopurinol* ; Animals ; Brain Infarction* ; Brain Ischemia ; Brain* ; Free Radicals ; Hydrogen Peroxide ; Infarction ; Infarction, Middle Cerebral Artery* ; Ischemia ; Middle Cerebral Artery* ; Oxidants ; Rats ; Superoxides ; Xanthine Oxidase

The intracranial arachnoid cysts are benign lesions in nature which thin walled cysts lying in relation to or enclosed by the subarachnoid space, filled with clear fluid. They may be due to developmental defects of the brain or meninges and secondary to inflammation, vascular occlusion or trauma but etiology of a large percentage is not well understood. In general, locations are near primary fissure and cisterns of the cerebral cortex and cerebellum. The clinical features and courses are unusual. Preoperative diagnosis is unlikely to be made but treatment is followed by good result. We had experienced two cases of intracranial arachnoid cysts which occurs in middle cranial fossa, and cerebral hemisphere. Pathologic diagnosis are arachnoid cysts.
Arachnoid Cysts* ; Brain ; Cerebellum ; Cerebral Cortex ; Cerebrum ; Cranial Fossa, Middle ; Deception ; Diagnosis ; Inflammation ; Meninges ; Subarachnoid Space

The authors present a case of oligodendroglioma in the 4th ventricle which is extremely rare in occurrence. The patient is 11 year-old male whose complaints were severe headache and vomiting. In the brain computes tomogram, hyperdense mass seated in the 4th ventricle with marked hydrocephalus. The patient had operation for removal of the tumor. In the operating field, there are no relation of the choroid plexus and specific vesselas but the tumor attached firmly on the floor of the 4th ventricle. The tumor was removed totally. The pathologic specimens were verified oligodendroglioma.
Brain ; Child ; Choroid Plexus ; Fourth Ventricle* ; Headache ; Humans ; Hydrocephalus ; Male ; Oligodendroglioma* ; Vomiting