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3.Eosinophilic, Polymorphic, and Pruritic Eruption Associated with Radiotherapy in a Patient with Parotid Gland Cancer.

Bookyoung KANG ; Ki Heon JEONG ; Min Kyung SHIN

Korean Journal of Dermatology 2014;52(8):591-592

7.Comparative Study on Development of Mouse Embryos in Three Commercial Media and Hatching Rates of Mouse Embryos with/without Pronase.

Jeong Heon LEE ; Hee Jeong GO ; Geu Jeong CHAE ; Ki Suk LEE ; Jong Duk KIM

Korean Journal of Fertility and Sterility 2001;28(3):235-246

OBJECTIVE: The purpose of this present study was to compare mouse embryo development in 3 commercial media and hatching competence of mouse embryo with or without enzymatic treatment. METHODS: Collected 375 mouse embryos were divided into three groups, and then cultured in IVF-20 (G2), Medicult IVF (M3), P-1 (blastocyst M), respectively. Three day mouse morulae were cultured in G2 media treated with pronase. The results were analyzed using Chi-square test, and considered statistically significant when p<0.01. RESULTS: The developmental rate of 2 cell mouse embryo after 72 hours was highest in IVF-20 (G2) among conventional 3 media. The hatching rate of mouse morulae was low when clultured in G2 media without pronase during 48 hours. However, it was higher when cultured in media treated with l mg/ml, 2.5 mg/ml, 5 mg/ml pronase, respectively. CONCLUSIONS: Using good media and digestion of zona pellucida with enzymatic treatment improve development and hatching rate of embryo. Therefore, implantation and pregnancy rate could be improved.
Animals ; Digestion ; Embryonic Development ; Embryonic Structures* ; Female ; Mental Competency ; Mice* ; Morula ; Pregnancy ; Pregnancy Rate ; Pronase* ; Zona Pellucida

1.Two Cases of Lichen Nitidus Treated with Topical 0.1% Tacrolimus.

2.A Case of Asymptomatic Plantar Angioleiomyoma.

3.Eosinophilic, Polymorphic, and Pruritic Eruption Associated with Radiotherapy in a Patient with Parotid Gland Cancer.

4.A Case of Leiomyoma of the Hand in a 6-year-old Boy.

5.A Case of Leiomyoma of the Hand in a 6-year-old Boy.

6.A Case of Lymphangioma-like Kaposi Sarcoma on the Sole

7.Comparative Study on Development of Mouse Embryos in Three Commercial Media and Hatching Rates of Mouse Embryos with/without Pronase.

8.A case of nocardiosis.

9.A Case of Dermoid Cyst on the Posterior Auriculocephalic Sulcus.

10.Pancreatoduodenectomy on periampullary cancer.

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