No abstract available.
Animals ; Intercellular Adhesion Molecule-1* ; Macrophages* ; Neutrophils* ; Rats* ; Urinary Bladder*

No abstract available.
Animals ; Intercellular Adhesion Molecule-1* ; Macrophages* ; Neutrophils* ; Rats* ; Urinary Bladder*

No abstract available.
Tissue Expansion Devices*

PURPOSE: The renal histological and hemodynamic changes and the expressions of hypoxia inducible factor-1alpha (HIF-1alpha) and vascular endothelial growth factor (VEGF) were evaluated according to the differential renal function (DRF) during total ureteral obstruction (TUO) in a rabbit model. MATERIALS AND METHODS: In forty-nine control (5) and 16 experimental rabbits (16 in TUO 3 days, 13 in TUO 7 days and 15 in TUO 14 days), the renal blood flow (RBF) and 99mTc-DTPA renal scan were measured both before and after TUO. The cut-off of the DRF group was 40%. The histological changes and expressions of HIF-1alpha and VEGF were evaluated using H&E and immunohistochemical stain, respectively. RESULTS: The entire control group demonstrated more than 40% DRF. Contrary to the control group, the DRF was less than 40% in 4 (25%), 7 (53%) and 6 rabbits (40%) in TUO 3, 7 and 14 day groups, respectively. The postobstructive compared to preobstructive RBF was decreased in each group. The RBF was more decreased in the lower than the higher DRF group (more than 40%) in all of the experimental groups. Abnormal histological changes were more prominent in the experimental groups, and increased with the obstruction time. However, there was no difference in relation to the DRF. The expressions of HIF-1alpha and VEGF were more prominent in the experimental and lower DRF groups. CONCLUSIONS: During acute TUO, the decreased RBF and hypoxia may play a role in preservation of the DRF.
Anoxia* ; Hemodynamics ; Hypoxia-Inducible Factor 1 ; Rabbits ; Renal Circulation ; Ureter* ; Ureteral Obstruction* ; Vascular Endothelial Growth Factor A*

PURPOSE: Intrinsic milieu of seminal plasma is important in maintaining the survival and fertilizing capacity of normal sperm. Nevertheless, there are still debates concerning the addition of seminal plasma during sperm preparation and cryopreservation. The purpose of this study is to investigate the effects of autogenous seminal plasma on the recovery of frozen fertile and infertile human sperm. MATERIALS AND METHODS: Semen from 10 fertile volunteers and 10 infertile patients was used. Sperm pellet from each group was resuspended and cryopreserved with 0.5ml cryoprotectant. Cryoprotectant was added with 100% (group I), 75% (group II), 50% (group III), 25% (group IV) and 0% (group V) seminal plasma according to each group. After 3 days, each vial was thawed and examined about sperm concentration, motility and morphology by CASA and Makler chamber. Sperm vitality was examined by eosin-nigrosin stain. RESULTS: The recovery rates of sperm motility and vitality after thawing in normal volunteers were higher in group I and II than other groups with statistical significance (p<0.05). However there was no difference between groups in sperm concentration and morphology (p>0.05). In the infertile patients all factors had no difference between groups. CONCLUSIONS: We concluded that the sperm quality following the addition of autogenous seminal plasma in cryoprotectant was improved in normal fertile men. This results may give a theoretical basis to support the clinical use and application for the seminal plasma to enhance the sperm quailty.
Cryopreservation* ; Healthy Volunteers ; Humans* ; Male ; Semen* ; Sperm Motility ; Spermatozoa* ; Volunteers

11β-hydroxysteroid dehydrogenase 1 (11β-HSD1) catalyzes the conversion of inactive cortisone into active cortisol, which has pleiotropic roles in various biological conditions, such as immunological and metabolic homeostasis. Cortisol is mainly produced in the adrenal gland, but can be locally regenerated in the liver, fat, and muscle. Its diverse actions are primarily mediated by binding to the glucocorticoid receptor. SW982, a human synovial cell line, expresses 11β-HSD type 1, but not type 2, that catalyzes the conversion of cortisone to cortisol. In this study, therefore, we investigated the control of lipopolysaccharide (LPS)-induced inflammatory responses by prereceptor regulation-mediated maintenance of cortisol levels. Preliminarily, cell seeding density and incubation period were optimized for analyzing the catalytic activity of SW982. Additionally, cellular 11β-HSD1 still remained active irrespective of monolayer or spheroid culture conditions. Inflammatory stimulants, such as interleukin (IL)-1β, tumor necrosis factor (TNF)α, and LPS, did not affect the catalytic activity of 11β-HSD1, although a high dose of LPS significantly decreased its activity. Additionally, autocrine effects of cortisol on inflammatory responses were investigated in LPS-stimulated SW982 cells. LPS upregulated pro-inflammatory cytokines, including IL-6 and IL-1β, in SW982 cells, while cortisol production, catalyzed by cellular 11β-HSD1, downregulated LPS-stimulated cytokines. Furthermore, suppression of NFκB activation-mediated pro-inflammatory responses by cortisol was revealed. In conclusion, the activity of cellular 11β-HSD1 was closely correlated with suppression of LPS-induced inflammation. Therefore, these results partly support the notion that prereceptor regulation of locally regenerated cortisol could be taken into consideration for treatment of inflammation-associated diseases, including arthritis.
Adrenal Glands ; Arthritis ; Cell Line* ; Cortisone ; Cytokines ; Homeostasis ; Humans ; Hydrocortisone ; Inflammation ; Interleukin-6 ; Interleukins ; Liver ; Oxidoreductases* ; Receptors, Glucocorticoid ; Tumor Necrosis Factor-alpha

Gout is a metabolic disorder manifested by hyperuricemia due to genetic or acquired diseases. The pathognomic lesion is the tophus which fine crystals of monosodium urate are deposited in articular carti- lage, synovia, tendon sheaths and other periarticular structures, epiphyseal bone, subcutaneous layers of the skin, and interstitial areas of the kidney. Frequent sites of tophaceous deposit are the external ear and subcutaneous deposits in the finger tips, palms and soles. Tophaceous gout involving the space of bipartite patella is very unusual and has not been reported before in Korea. So, we report a case of 38 year old male patient who presented with tophaceous gout involving the space of bipartite patella with review of literatures.
Adult ; Ear, External ; Fingers ; Gout* ; Humans ; Hyperuricemia ; Kidney ; Korea ; Male ; Patella* ; Skin ; Synovial Fluid ; Tendons ; Uric Acid

No abstract available.
Epstein-Barr Virus Infections* ; Herpesvirus 4, Human* ; Humans ; Lymphoma*

BACKGROUND: Transcatheter closure of patent ductus arteriosus, using the Rashkind double umbrella occluder system, had been attempted in mulitcenter since non-surgical closure of patent ductus arteriosus by Rashkind. METHODS: Between July 1991 and June 1993, transcatheter closure of patent ductus arterious was attempted in 20 adult patients(21 trials). RESULTS: Seventeen female and 3 male patients was consisted of the study. The patient age ranged from 17 to 54 years(mean 30+/-9 years). Mean pulmonary artery pressure before closure was 18.5+/-6.0mmHg(range from 10mmHg to 30mmHg). The diameter of ductus ranged from 3.5mm to 8.0mm(mean 5.2+/-1.5mm), as determined by contrast injection through 11F Mullin sheath or 7F catheter. There was significant decrease of Qp/Qs from 2.6+/-1.1 to 1.5+/-0.4 immediately after transcatheter closure of ductus(p<0.01). There was significant decrease of left ventricular end-diastolic dimension by echocardiogram from 58.7+/-7.6mm to 53.6+/-6.5mm after transcatheter closure of ductus(p<0.01). Among the 21 cases, 16 cases(76.2%) had the clinical improvement without the support of surgical closure. One 17mm Rashkind umbrella was retrieved because of position and problem of deployment. There was no device embolic experience in 21 cases. There were 2 cases of hemolytic anemia during the follow-up period ; one case was surgically ligated and another 17mm device was implanted in the other case. CONCLUSIONS: Transcatheter closure of patent ductus arteriosus in adult patients can replace the surgical correction in selected patients.
Adult* ; Anemia, Hemolytic ; Catheters ; Ductus Arteriosus, Patent* ; Female ; Follow-Up Studies ; Humans ; Male ; Pulmonary Artery

A 23 months-old girl visited the hospital because of fever and left neck mass. She was diagnosed as acute suppurative thyroiditis with piriform sinus fistula. Thyroid sonography showed perithyroidal abscess formation and thyroid scan showed decreased uptake of Tc-99m pertechnate of both thyroid glands. Magnetic resonance imaging of neck showed abscess cavity extending to the swollen left thyroid gland. And there was tiny fistula between thyroid and piriform sinus in the barium esophagogram. Streptococcus gordonii was isolated on needle aspiration culture. We report a case of piriform sinus fistula of the neck complicated with suppurative thyroiditis. The fistula was treated with chemocauterization using trichloroacetic acid.
Abscess ; Barium ; Female ; Fever ; Fistula* ; Humans ; Magnetic Resonance Imaging ; Neck ; Needles ; Pyriform Sinus* ; Streptococcus gordonii ; Thyroid Gland ; Thyroiditis, Suppurative* ; Trichloroacetic Acid*