No abstract available.
Lichen Nitidus* ; Tacrolimus*

The author evaluated the optimal concentration of 3 compositions of TIC medium which has used as the melanacytes culture medium. The concentrations of placental extract and bovine pituitary extract, which have the ability to promote proliferation of melanocytes, were evaluated also. Modified TIC medium with above 5 components of evaluated concentration was very effective in melanocytes culture. The results were as follows : l. 12-0-tetradecanoyl-phorbol-13-acetate (TPA) showed effective melanocytes proliferating activity at the concentration of 30ngml (p(0.05) 2. Isobutylmet:hyl xanthine (IBMX) showed effective melanocytes proliferating activity at the concentration of 0.3mM (p(0.05) 3. Cholera toxin (CT) showed effective melanocytes proliferating activity at the concentration of )OnM (p(0.05) 4. Two percentages of placental extract in culture medium showed effective melanocytes proliferating activity. S. Two percentages of bovine pituitary extract in culture medium showed effective melanocytes proliferating activity. 6. Placental extract and isobutylmethyl xanthine proved to have high melanocytes proliferating activity. 7. Melanocytes proliferated rapidly on modified TIC medium (Proliferation doubling time . about 43 hours) 8. The peak time of melanocytes proliferation (7.2 X 10/cm) was observed on the seventh day of culture, From this data, this culture system can be recommended as a new melanocytes culture.
Cholera Toxin ; Humans* ; Melanocytes* ; Tics ; Xanthine

No abstract available.
Giant Cells* ; Granuloma, Giant Cell*

No abstract available.
Axilla ; Poroma*

INTRODUCTION: The fixation strength of transpedicular screw system in the vertebral hody relied on bone quality and anatomical characteristics of vertebral pedicle, designs of screw and types of connection(rod or plate) with screw. The purpose of this study is to verify the biomechanical nature of the transpedicular fixation in spine under various conditions with porcine vertebrae. MATERIAL AND METHOD: Fresh porcine vertebrae and the custom-made screws were used in this experiment. To reduce the errors caused by vertebral bodies of different size and quality, vertebral bodies having regular range of pedicular width(10.0 to 11.5mm) and hone density(more than 1.0 gm/cm2) were used. The pedicle screws were inserted in the same procedure and axial pull out test was performed with using the Material Testing System(lntron8511, Canton, USA). The experiments were performed in four types to assess the difference of strength accroding to designs of the screw hy using two group of screws. The first group of screw was designed according to the outer and inner diameter and the second group was designed according to the shape, pitch, and thread profile of screw. Experiment I was perfomed to evaluate the effect of screw diameters on the biomechanical pull-out strength hy using the first group of custom-made pedicle screw which fixed all other factors except the diameter of screw. Experiment I was to verify the effect of screw shape, experiment III to verify the effect of pitch and experiment IV to verify the effect of thread profile. RESULTS: The results of experiments were summarized as follows: Experiment I showed that the screw of larger outer diameter had greater holding strength. Experiment II showed that the holding strength of cylindrical shaped screw is superior to that of conical shaped screw. Experiment III showed that there is no statistical significance between different modes of pitch. Experiment IV showed that the holding strength of buttress shape of thread profile is superior to that of V-shape. CONCLUSION: It seemed that the fixation strength of the screw was more powerful with 1 mm increment of outer diameter in 4-7mm of outer diameter, 3mm of pitch and buttress shape of thread of the screw with the same operation technique.
Spine

No abstract available.
Hyperpigmentation ; Paget Disease, Extramammary* ; Scrotum*

This study was done to identify the association between serum copper and zinc levels and the cirrhosis and hepatocellular carcinoma(HCC), and to evaluate its diagnostic value on liver diseases. Sixty-three healthy persons, 60 patients with cirrhosis and 33 patients with hepatocellular carcinoma were rendomly selected and investigated for their general characteristics from October 1990 to August 1991. For analysis of the biochemical markers in liver function test and the serum copper and zinc levels, their fasting venous blood were sampled at 9:00 to 11:00 in the morning and centrifuged to separate the serum within one hour. All the samples were immediately analysed for biochemical markers and stored at -20 C in polypropylene tubes further copper and zinc analysis. Mean of serum copper levels was 91.97+/-4.76 microgram/dl in control, 106.21+/-2.73 microgram/dl in cirrhosis and 127.05+/-0.77 microgram/dl in HCC. The value of HCC was statistically significantly higher than that of the control and cirrhosis(p<0.05). Serum zinc levels were 110.82+/-7.24 microgram/dl in control, 68.10+/-5.43 microgram/dl in cirrhosis and 63.78+/-2.20 microgram/dl in HCC. The values of cirrhosis and HCC were statistically significantly different among three groups(p<0.05). Test total protein, albumin, ALP and total bilirubin of biochemical markers of liver function were statistically significantly different among three groups(p<0.05). Differences between cirrhosis and HCC for ALT and AST, and between the control and HCC for direct bilirubin were not statistically significant. Biochemical markers statistically significantly correlated with serum copper and zinc levels and Cu/Zn ratio(p<0.05), were variable in three groups. In multiple logistic regression, odds ratio of serum copper level and Cu/Zn ratio had no statistically significance on the cirrhosis and the HCC, but that of serum zinc was statistically significant as 0.951 and 0.952 (p<0.05). Serum copper and zinc levels and Cu/Zn ratio were not statistically significantly different between the cirrhosis and HCC. Albumin, ALP, zinc, total bilirubin and age among all variables were selected as main variables for three-group discriminant analysis. Percentage of "grouped" cases correctly classified by these five variables was 98.4 for control, 73.4 for cirrhosis, 75.7 for HCC and 84.0 for all subjects. This study suggests that zinc may has an independently inhibitory effect on the liver disease and serum zinc level is considered to play a role as diagnostic marker on the hepatic disorders and be more useful than serum copper level and Cu/Zn ratio in diagnosis of the liver diseases.
Bilirubin ; Biomarkers ; Carcinoma, Hepatocellular* ; Copper* ; Diagnosis ; Fasting ; Fibrosis ; Humans ; Liver Cirrhosis* ; Liver Diseases ; Liver Function Tests ; Liver* ; Logistic Models ; Odds Ratio ; Polypropylenes ; Zinc*

We had experience of 464 cases of anesthesia for repair of cleft lip and cleft palate under 10 years of age. The analyzed results are as follows: 1) The 464 cases were divided into 5 age groups and 165 cases(35.6%) were in the under 2 years age group. Sex distribution was 291 males(66.2%) and 173 females(37.3%) 2) 377 cases(81.3%) were under 20kg of body weight. 3) There were 229 cleft lip cases (29.3%), 233 cleft palate cases, and 1 case each of (50.2%), of naso-ocular fistular correction and oro-ocular correction. 4) There were 386 cases elective surgery and 2 were emergency cases. 5) All cases had general anesthesia by means of endotracheal intubation 377 cases(81.3%) with a non-rebreathing system and 87 cases(18.8%) had a semi-closed absorption system. 6) The main anesthetic was halothane in 462 cases(99.6%) and Valium-Demerol and ether was 1 case each. 7) The mean blood loss in palatoplastry was 10ml/kg of body weight but it was 13.7ml/kg in the 5~10kg of body weight group, 11.6ml/kg in the 10kg~20kg of body weight group and 7.5ml/kg in the 20~30kg of body weight group. 8) Incidence of cleft lip combined with cleft palate was 211 cases(45.5%) among 464 cases and 149 cases(51.2%) were males and 62 cases(35.8%) were females. 9) The most common type of cleft lip was left incomplete lip(30.1%) and of cleft palate it was left complete palate(30.5%). 10) Familial history was found in 56 cases(12%).
Absorption ; Anesthesia* ; Anesthesia, General ; Body Weight ; Child* ; Cleft Lip ; Cleft Palate ; Emergencies ; Ether ; Female ; Halothane ; Humans ; Incidence ; Intubation, Intratracheal ; Male ; Sex Distribution

We performed the interscalene technic of brachial plexus block for an operation of the upper extremity in 94 patients. We used 1%, 1.5% or 2% lidocaine solution with or without 1:200,000 epinephrine. There were paresthesias in all cases of needle puncture with the interscalene approach. The resutls were as follows: 1) Onset of anesthesia was observed in 3~10 minutes(average: 5 min.) 2) Complete anesthesia was observed in 10~30 minutes(average: 18 min.) 3) The mean interval of anesthesia to operation was 22 minutes. 4) We observed satisfactory blocks in 81 cases, unsatisfactory block in 8 cases and failure in 5 cases. 5) There were not any complications such as pneumothorax, dyspnea, convulsions, subarachnoid or epidural injection, etc.
Anesthesia ; Brachial Plexus* ; Dyspnea ; Epinephrine ; Humans ; Injections, Epidural ; Lidocaine ; Needles ; Paresthesia ; Pneumothorax ; Punctures ; Seizures ; Upper Extremity

BACKGROUND: Subungual exostosis is a rare, solitary, benign bony tumor of the distal phalanx. It occurs mainly in the great toe and less frequently in the other toes and fingers. It may cause nail deformity and pain. OBJECTIVE: We wanted to describe the clinical, histopathological and radiological features and the recurrence after treatment of subungual exostosis. METHODS: A total of 12 patients who were diagnosed with subungual exostosis at our hospital from 1997 through 2007 were evaluated. RESULTS: There were seven females and five males. The average age of the patients at the time of surgery was 24.2+/-9.2 years. The mean duration of symptoms was 11+/-6.0 months. All the patients presented with a solitary, fixed, hard exophytic tumor beneath the nail plate. Eight patients (67%) presented with a lesion on the great toe, three patients (25%) with a lesion on the other toes and one patient (8%) with a lesion on the thumb. The histopathology revealed a fibrocartilaginous cap, enchondral ossification and calcification in all the patients and islands of mature trabecular bone in ten patients (83%). The radiographic picture showed a typical pattern of a bony excrescence arising from the dorsal aspect of the distal phalanx. All the patients were treated with surgical excision of the lesion and eleven patients (92%) showed good results. One patient (8%) had a local recurrence and she underwent subsequent excision. No additional local recurrences were observed. CONCLUSION: Subungual exostosis is unfamiliar to most dermatologists, and it can be difficult to diagnose in some cases. When a subungual mass is observed, subungual exostosis should be considered in the differential diagnosis and subsequent radiographic studies along with histologic studies are recommended.
Congenital Abnormalities ; Diagnosis, Differential ; Exostoses ; Female ; Fingers ; Humans ; Islands ; Male ; Nails ; Recurrence ; Thumb ; Toes