1.Expressions of Vascular Endothelial Growth Factor (VEGF), Phospholipase C-gammal and Ki-67 in Squamous Cell Carcinoma and High Grade Squamous Intraepithelial Lesion of Uterine Cervix.
Korean Journal of Pathology 1998;32(4):290-297
Angiogenesis is an early event in tumorigenesis and facilitates tumor progression and metastasis. This study was performed to evaluate the expression of vascular endothelial growth factor (VEGF), phospholipase C-gamma1 (PLC-gamma1) and Ki-67, and to assess the relationship between them in cervical squamous cell neoplasia. The materials were fifty cervical squamous cell lesions, consisted of thirty HSIL (6 moderate dysplasia, 11 severe dysplasia, 13 carcinoma in situ), and twenty invasive squamous cell carcinoma (ISCC) cases. Immunohistochemical stain for VEGF, PLC-gamma1 and Ki-67 were done. Expression rate of VEGF was significantly higher in ISCC than in HSIL (p=0.012). PLC-gamma1 expression was significantly higher in ISCC than in HSIL (p=0.004). Ki-67 labelling index was significantly higher in ISCC than in HSIL (p=0.001) and higher in VEGF-positive tumors than in VEGF-negative tumors (p=0.018), but there were no significant differences between PLC-gamma1 expression and Ki-67 labelling index (p>0.05), and between PLC-gamma1 and VEGF (p>0.05). This study suggests that PLC-gamma1 and VEGF may play an important role in tumor cell proliferation and invasion, and these may be a useful marker to predict the possibility of invasion in cervical cancer.
Carcinogenesis
;
Carcinoma, Squamous Cell*
;
Cell Proliferation
;
Cervix Uteri*
;
Female
;
Neoplasm Metastasis
;
Phospholipases*
;
Uterine Cervical Neoplasms
;
Vascular Endothelial Growth Factor A*
2.Expression of p27Kip1 Protein in Carcinoma of the Urinary Bladder.
Korean Journal of Pathology 2000;34(5):341-348
The cyclin-dependent kinase (cdk) inhibitor p27Kip1 gene is a powerful molecular determinant of cell cycle progression. Loss of expression of p27Kip1 has recently been shown to be predictive of disease progression in several human malignancies. The prognostic value and expression of p27Kip1 have been incompletely studied in bladder cancer. In this study, we investigated the relationship between p27Kip1 protein expression and clinicopathologic parameters in 50 cases of carcinoma of the urinary bladder by conducting immunohistochemical analysis and DNA flow-cytometry. Malignant bladder tissue demonstrated a heterogeneous pattern of p27Kip1 immunoreactivity. In addition, there was progressive loss of expression with increasing tumor grade. The expression of p27Kip1 protein was unrelated to stage, DNA ploidy and S phase fraction (SPF). SPF was unrelated with tumor grade and DNA ploidy. The results indicate that p27Kip1 is frequently expressed in well differentiated transitional cell carcinomas of the urinary bladder but less often expressed in muscle-invasive transitional cell carcinomas. The expression of p27Kip1 and its prevalence in low-grade tumors may reflect growth regulatory influences and potential inhibiting action in tumor progression and novel predictive markers of the biological potential of bladder tumors.
Carcinoma, Transitional Cell
;
Cell Cycle
;
Cyclin-Dependent Kinase Inhibitor p27*
;
Disease Progression
;
DNA
;
Humans
;
Phosphotransferases
;
Ploidies
;
Prevalence
;
S Phase
;
Urinary Bladder Neoplasms
;
Urinary Bladder*
3.Castration-induced Apoptosis in the Rat Prostate.
Korean Journal of Pathology 1998;32(6):431-442
This study was carried out to investigate the morphologic findings and process of castration-induced apoptosis in the rat prostate. The experimental group was treated with bilateral orchiectomy followed by sequential sacrifices at 1, 2, 3, 4, 5, 6, 7 days and 2, 3 weeks (6 rats, respectively). Ventral prostate was extirpated and examined by light microscopic and immunohistochemical, ultrastructural observation. Apoptotic index increased by 4 days after castration and decreased thereafter. ApopTag stain revealed brownish granular pattern in the nucleus of apoptotic cells. DNA fragmentation rate was 0.5% in the control group and began to increase by 1 day after castration and reached to 11.1% by 4 days and decreased thereafter. PCNA stain showed brownish granular pattern in the nucleus of some epithelial cells of the prostatic glands. PCNA labelling index was 2.4% in the control group and reached peak by 3 days after castration and decreased thereafter. Electron microscopically, there was chromatin condensation with margination toward the nuclear membrane by 1 day after castration. Also noted were condensation of cytoplasm, dilatation of RER and nuclear fragmentation. Apoptotic bodies were formed and phagocytosed by adjacent cells and some apoptotic bodies were found in the lumen of acini. Based on these results, it can be concluded that castration-induced prostatic involution is the result of apoptosis. Detection of DNA fragmentation with ApopTag is a more a accurate method to identify not only apoptotic body formation itself but also the previous step of apoptotic body formation. PCNA labelling index to identify the cellular proliferation seems to play an active role in the early step of apoptosis and be a good tool for investigation of apoptosis.
Animals
;
Apoptosis*
;
Castration
;
Cell Proliferation
;
Chromatin
;
Cytoplasm
;
Dilatation
;
DNA Fragmentation
;
Epithelial Cells
;
Nuclear Envelope
;
Orchiectomy
;
Proliferating Cell Nuclear Antigen
;
Prostate*
;
Rats*
4.Surgical management of pulmonary tuberculosis: a review of 3,566 cases.
Ki Jung KWON ; Eung Soo KIM ; Tae Won KWON
The Korean Journal of Thoracic and Cardiovascular Surgery 1992;25(5):480-493
No abstract available.
Tuberculosis, Pulmonary*
5.Four Cases of Gianotti-Crosti Syndrome.
Yong Gab KWON ; Ki Sup CHUNG ; Kir Yong KIM
Journal of the Korean Pediatric Society 1984;27(8):820-826
No abstract available.
Acrodermatitis*
6.Adenocarcinoma of Urinary Bladder: 2 cases report.
Ki Kwon KIM ; Eunk Sook CHANG ; Chai Hong CHUNG
Korean Journal of Pathology 1988;22(4):456-461
Two cases of adenocarcinoma of the urinary bladder with clinical and pathological features, and brief review of the literatureare presented. Case 1: The patient, a 52 year-old man, was admitted to this hospital because of intermittent painless total gross hematuria for 15 years. Cystoscopy was done, and showing a cauliflower mass with broad based diffuse infiltrating lesion at the right anten or portion of bladder. TUR-B was performed. Microscopically, the lesion consisted of colonic metaplastic epithelium with atypical glands and cystic dilatation and adenocarcinoma. Case 2: The patient, a 52-year-old woman, was admitted to this hospital because of total painless gorss hematuria for 1 year. Cystoscopy was done showing a sessile diffuse mass with ulceration on the dome area. Total cystectomy was performed. Grossly, the tumor showed an ulcerative tumor mass with elevated nodular margin at the dome of the bladder. Microscopically, the lesion consisted of anaplastic glands with back to back arrangement and branching glands through the entire thickness of the bladder wall.
Female
;
Humans
;
Adenocarcinoma
7.The effect of electroconvulsive therapy on the cerebrospinal fluid 5-HT and 5-HIAA concentrations in the chronic treatment-resistant schizophrenia.
Jun Soo KWON ; Ki Woong KIM ; Jong Inn WOO
Journal of Korean Neuropsychiatric Association 1992;31(5):846-855
No abstract available.
Cerebrospinal Fluid*
;
Electroconvulsive Therapy*
;
Hydroxyindoleacetic Acid*
;
Schizophrenia*
;
Serotonin*
8.PUVA Therapy of Pityriasis Lichenoides Chronica.
Hae Ki HAN ; Jin Kwon KIM ; Hong Il KOOK
Korean Journal of Dermatology 1982;20(3):413-417
Pityriasis lichenoides chronica is characterized by unknown etiology, chronicity and by being essentially asymptomatic and refractory to therapy. Nine patients with pityriasis lichenoides chronica were treated with orally administrated 8-Methoxypsoralen and UVA irradiation(PUVA Therapy). After S-45 PUVA treatments, lesions were completely cleared.
Humans
;
Methoxsalen
;
Pityriasis Lichenoides*
;
Pityriasis*
;
PUVA Therapy*
9.Four cases of laryngeal saccular cyst.
Seong Woan KIM ; Ki Hwan HONG ; Sam Hyun KWON
Korean Journal of Otolaryngology - Head and Neck Surgery 1991;34(2):393-398
No abstract available.
10.The outcome of pregnancy following renal transplantation.
Yoo Sun KIM ; Ki Bum KWON ; Chang Kwon OH ; Hye Jung YUN ; Yong Won PARK ; Ki Il PARK
The Journal of the Korean Society for Transplantation 1993;7(1):149-155
No abstract available.
Kidney Transplantation*
;
Pregnancy*