BACKGROUND: Numerous studies demonstrated that genistein induced the decrease of proliferation and apoptosis in a variety of cells. However, there is no report about the effect of genistein on proliferation and demise of mast cells. OBJECTIVE: This study was conducted to investigate genistein-induced aoptosis of mast cells as it pertains to both its basic drug mechanism and the potential therapeutics of the pathologic conditions accompanying mast cell proliferation. METHODS: p815 murine mastcytoma cell line was used to assess the effects of genistein treatment including viability and proliferation, morphlolgic study, DNA electrophoresis, the effect of caspase inhibitor, western blotting, and mitochondrial event. RESULTS: Genistein indeced many apoptotic manifestations as evidenced by changes in cell morphology, generation of DNA fragmentation, activation of caspase 3, and DNA hypoploidy. The reduction of mitochondrial membrae potential and the release of cytochrome c to cytosol were also demonstrated. However, reduction of mitochondrial membrane potential and cytochrome c release were not prevented by caspase inhibitors zVAD-fmk and BocD.fmk, or PTP(permeability transition pore) blockers such as bongkrekic acid and cyclosporin A. CONCLUSIONS: This in vitro study suggests that pathologic increases in mast cell number possibly be regulated in vivo by therapeutic strategy enhancing apoptosis by treatment of genistein.
Apoptosis* ; Blotting, Western ; Bongkrekic Acid ; Caspase 3 ; Caspase Inhibitors ; Cell Line ; Cyclosporine ; Cytochromes c ; Cytosol ; DNA ; DNA Fragmentation ; Electrophoresis ; Genistein ; Mast Cells ; Mastocytoma* ; Membrane Potential, Mitochondrial

The authors present a 3-year-old boy showing generalized migratory erythema with doubleedged scaling broders since 3 month after birth, Histologic examination revealed an acanthotic epidermis with hyperkeratosis, focal parakeratosis and papillomatosis. The PAS-positive, diastase-resistant amorphous materials were found in th space of parakeratoric scales and cytoplasms of spinous cells just below the parakeraroric lesion. On clectron microscopy, we could observe the round dense bodise in the upper spinous cells and a number of lipoid granules in the horny layer. Our patient showed characteristic clinical, clinical, microscopic and ultrastructural gearures of ichthyosis linearis citcumflexa(ILC). However, he showed no hair shaft abnormalities, which is the common finding of Metherthon/sysdrome(NS) along with ILC and atopic deathesis.
Child, Preschool ; Cytoplasm ; Epidermis ; Erythema ; Hair ; Humans ; Ichthyosis* ; Male ; Microscopy ; Papilloma ; Parakeratosis ; Parturition ; Weights and Measures

No abstract available.
Colon* ; Colonic Neoplasms* ; Humans ; Sparganosis* ; Ultrasonography*

No abstract available.
Methotrexate* ; Skin*

No abstract available.
Nevus*

Pyoderma gangrenosum (PG) is a rare, painful, chronic, ulcerating skin disease of an unknown etiology. It can be associated with systemic diseases such as Crohn's disease, ulcerative colitis, myeloproliferative disease, monoclonal gammopathy, rheumatoid arthritis and Behcet's disease, or after minor trauma and surgery. To the best our knowledge, no cases of PG associated with pregnancy have ever been published in the Korean medical literature, and there have been only ten such reported cases in other countries. Herein, we report on a case of PG that developed during pregnancy in a patient with rheumatoid arthritis.
Arthritis, Rheumatoid ; Colitis, Ulcerative ; Crohn Disease ; Humans ; Paraproteinemias ; Pregnancy ; Pyoderma ; Pyoderma Gangrenosum ; Skin Diseases ; Ulcer

Ankyloblepharon, Ectodermal defects, and Cleft lip and palate (AEC) syndrome, also known as Hay-Wells syndrome, is a rare form of ectodermal dysplasia initially described by Hay and Wells in 1976. It is an autosomal dominant disorder caused by mutations in the gene encoding the TP63 transcription factor, specifically in the Sterile Alpha Motif (SAM) domain. It is characterized by congenital ectodermal dysplasia, including alopecia, scalp infections, dystrophic nails, hypodontia, ankyloblepharon and cleft lip and/or cleft palate. To our knowledge, no case of AEC syndrome associated with a TP63 gene mutation has ever been published in Korea. Herein, we present a Korean female infant with AEC syndrome due to a novel TP63 gene mutation.
Alopecia ; Anodontia ; Cleft Lip ; Cleft Palate ; Ectoderm ; Ectodermal Dysplasia ; Eye Abnormalities ; Eyelids ; Female ; Humans ; Infant ; Korea ; Nails ; Palate ; Scalp ; Transcription Factors

Darier's disease is a well-known genodermatosis characterized by recurrent waxy, hyperkeratotic papules usually occurring over the seborrheic area. The major histopathological changes are characteristic acantholysis and dyskeratosis which are diagnostic of the disease with typical clinical features. There are less common variants including the hypertrophic, vesicobullous and linear type. However, comedonal lesions are very rare. We report an unusual case of Darier's disease, which showed prominent comedonal papules and plaques over the face, scalp and upper trunk with the typical findings of Darier's disease.
Acantholysis ; Darier Disease* ; Scalp

No abstract available.
Onychomycosis

No abstract available.
Candida ; Onychomycosis