Introduction: Perennial plant 15-60 cm high with slim creeper strong roots1-4. Dianthus superbus usually grows in a larch and Siberian Pine forest, in its meadow, edge and glade, bank of the river and thick bushes forests covering Khuvsgul, Khentii, mountainous and fertile pastures, Mountain range, Khovd and Mongol Altaic Mountain range1,2,4. The study of biological activity and bioactive compounds of plants, used in Mongolian and Tibetan traditional medicine such as Dianthus superbus and further enrichment of Mongolia’s drug foundation by natural drugs which have low toxic and toxicity effects, are one of the important goals of scientist. Upper part of the land is used for liver blood fever, blood pneumonia, menopause, kidney diseases, cystitis, nephritis and edema Mongolian traditional medical treatment and uterus muscle tightening action3,4.Goal: The aim is to study the phytochemical properties of Dianthus superbus upper part of the land.Materials and Methods: We performed the research taking material assistance of pharmacognozy laboratories in Monos university from 2012-2013. The quantitative and qualitative analyses of plant bioactive compounds were evaluated by Russian XI Pharmacopeia and the method according to Mongolian National Standard5. Result of the study was developed by SPSS 16 and End notе programme.Result: The quantitative and qualitative analyses of plant bioactive compounds of Dianthus superbus upper part of the land were evaluated by Russian XI Pharmacopeia and the method according to Mongolian National Standard and tannin, coumarin, flavonoid and naturally active substances were found.Conclution: It was carried out phytochemical investigation of the Dianthus superbus using spectrophotometer as a result we determined that if contains 10.5 % of sum flavonoid, 7.45 % coumarin. Amount of biological active compound-tannins was4.24 %.

Background Diabetes is a metabolic disorder that is characterized by chronic high blood glucose levels that causes complications in the eyes, kidneys, heart, vessels and nerves. Currently diabetic nephropathy is the most significant long-term complications in terms of morbidity and mortality for individual patients with diabetes. Honey bee venom can be considered as a natural remedy for diabetes due to its blood glucose levels lowering and lipid-regulating effect on diabetic rabbits. Aim The aim of this study was to investigate the effect of Mongolian honey bee venom (Apis mellifera) on renal dysfunction in alloxan induced diabetic rabbits. Material and Method Twenty two Chinchilla rabbits were divided into three groups: control (n=6), diabetic (n=8), and bee venom treated (n=8). The diabetic group was injected with 5% solution of Alloxan monohydrate 100 mg/kg intravenously behind the ear for 2 minutes to induce diabetes. The bee venom treated group received a bee sting (a sting contains 0.2-0.5 ml of bee venom) on their hind paw every day after their diagnosis of diabetes. Result Bee venom treatment (BVT) led to the following changes: compared to the diabetic group, the bee venom treated group’s blood glucose levels lowered between 14.9% and 26.5%; the plasma creatinine and urea levels were decreased respectively by 19,8% and 14.8%. Blood urea nitrogen (BUN) levels were reduced by 14.8%. Conclusion: Treatment with Mongolian bee venom lowered the blood glucose levels and prevents the renal dysfunction in alloxan induced diabetic rabbits

Introduction In 19th century, researchers proved at biochemical level the healing properties of bee products such as bee venom, honey, royal jelly, pollen, propolis and wax. The object of our research is the Apis cerena’s venom properties1-2. Asiatic honey bee or Apis cerana is small honey are small honey bees of southern and southeastern Asia, such as China, India, Japan, Malaysia, Nepal, Bangladesh and Papua New Guinea3. This species is also known as the Himalayan hive honeybee. This species is the sister species of Apis koschevnikovi, and both are in the same sub¬genus as the Western (European) honey bee, Apis mellifera4. Goal The purpose of our research is to study property and potential of bee venom and its effect on immune system. Heal¬ing property of Apis cerana was high. This study proves that bee venom therapy stimulates immunity. Materialis and Methods The research was conducted at the Scientific Research Center of “Monos” Institute of Traditional Medicine and in biochemical Laboratory of “Khuljborjigon” Clinic. For the experiment, we used 23 perfectly healthy mice of same sex and size which meets standards of laboratory testing. We put a bee sting to 0.5 ml of 10% red blood cell (RBC) solution and measured time of heamolysis to de¬fine bee venom potential/capability by Shkenderov S., Ivanov Ts., (1985) method. Following Erne (1963), Kovalev I.E.,(1976), Petrov’s (1980) methodology of studying effects on immune system, we have stung bee venom to 23 mice on the acupuncture point of hind paw every other day in total 3 times. On third day of the experiment, we in¬jected into vein 2ml of 10 % sheep’s RBC to stimulate the immunity. On the fifth day, we defined weight of pancreas, number of pancreatic cells, pancreatic index, and haemagglutination titre. Results Potential of bee venom is determined by speed of heamolysis when bee sting is placed in the 0.5 ml of 10% RBC solution. If we place one bee sting into 1ml of RBC solution then the speed of heamolysis is 46 seconds, when two stings are place speed is 38 seconds and when 3 stings placed then time is 30. Compare to usual speed of heamolysis which is 60 seconds, change in time depending on the number of bee stings proves the effectiveness of bee venom (Table 1). In figure 3, the number of spleen cells of control group’s was 142.71±55.51*106/ml. this is 1.2 times lower compare to normal group which is 172.67±135.5*106/ml. BVT group’s number of spleen cells was 329.78±187.78*106/ml and 1.61 times bigger than in control group. In comparison to control group, haemagglutina¬tion titre of BVT group was 1.13 times higher (BVT group 54.86±19.95%; control group 50±8.83%, p<0.05) and this indicates that BV has immunity stimulating effect. Conclusions From our experiment we can conclude the following 1. Apis mellifera’s bee venom has high treating effect. 2. Bee venom therapy has immunity stimulating activity.

Introduction: Air pollution has become one of the major problems in socio-economic and health issues in Mongolia. Among the various hazards of particulate matter (PM) pollutants, microorganisms in PM2.5 and PM10 are thought to be responsible for various allergies and for the spread of respiratory diseases. Recent studies have shown that PM2.5 particles can cause chronic heart failure, heart arrhythmias, and strokes, as well as lung damage, cirrhosis, inflammation, cancer, cardiovascular disease, and metabolic disorders. Furthermore, some studies have concluded that PM2.5 particles in the environment are a risk factor for gastrointestinal, liver, colon, and lung cancer as well as it affects the growth and metastasis of various cancer cells caused by other factors. In our country, the health effects of air pollution and the relationship between the pathogenesis of cancer research are scarce. Therefore, the study of the effects of PM2.5 particles on cancer cell proliferation, migration (metastasis) can provide a significant role for cancer treatment, diagnosis, and prevention. Purpose: Determining the effects of PM2.5 particles on cancer cell proliferation, migration (metastasis) in in-vitro Material and Methods: A human liver cancer cell line (HepG2), human gastric cancer cell line (AGS) were obtained from the central scientific research laboratory in the Institute of medical sciences. HepG2, AGS cells were seeded at a concentration of 1*105 cells/mL in a culture flask and cultured in RPMI-1640 medium supplemented with 10% FBS, 1% antibiotic mix (penicillin, streptomycin) in a humidified atmosphere of 5% CO2 at 37 °C. The cytotoxic effect of PM 2.5 in AGS, HepG2 cells were evaluated by MTT, CCK8 assays. AGS, HepG2 cells were incubated in 96 well plates for 24h then treated with different concentrations (0, 5, 10, 25, 50 and 100 μg ) of Bayankhoshuu, Buhiin urguu, and Zaisan samples for 24h, respectively. Results: Concentrations of 10, 25, and 50 μg/ml of samples collected from the Bukhiin urguu and Zaisan in March increased HepG2 cell growth, while doses of 25, 50 μg/ml of samples collected from Bayankhoshuu in March and December increased HepG2 cell growth. Therefore, concentrations of 25 and 50 μg/ml of samples collected from Bayankhoshuu in March increased AGS cell growth, while concentrations of 25, 100 and μg/ml of samples collected in December increased AGS cell growth. However, no cytotoxic effect was observed in the sample collected from Zaisan in March, whereas the PM2.5 sample enhanced AGS cell growth in dose dependent manner in December.(p <0.05) Conclusion High levels of heavy metals were detected in samples collected in December from Bayankhoshuu, Bukhiin urguu and Zaisan of Ulaanbaatar. Concentration of 25 μg/ml of samples collected from the Bukhiin urguu and Zaisan in March increased HepG2 cell growth. Concentrations of 25 μg/ml of PM2.5 collected from three regions around Ulaanbaatar increased HepG2 and AGS cell migration.