Blood fed An.dirus collected from Khanh Phu commune, Khanh Vinh district, Khanh Hoa province were reared and forced to lay eggs in the insectary of NIMPE. Parous females fed with mice blood were found to easy to lay eggs from 3-5 batches more. The conditions for image development of image stages were found to be as follows: temperature from 23 - 26oC, light time 12 – 24 hour with 90 – 150 Lux; the density of larvae is 1/5.7 cm2 rearing tool surface. Mosquitoes survived from 2 – 28 days. The temperature of 23 – 26oC and humidity of 70 –70 % were determined to be optimal for development of mosquitoes
Laboratories ; Biological Products ; Culicidae ; diagnosis ; Diptera

Background: Currently, tracking, monitoring insecticide resistance of mosquitoes transmitting disease is an urgent need.\r\n', u'Objectives: To evaluate resistance to insecticide of three mosquito species: Aedes Aegypti, Culex quinquefasciatus and culex tritaenyorhynchus in Ha Noi\r\n', u'Subject and method: Aedes Aegypti, Culex quinquefasciatus culex tritaenyorhynchus mosquito species was collected from some districts of Ha Noi city. These mosquito species was tested sensitivity with 4 insecticides: permethrin 0.75%, lambda-cyhalothrin 0.05%, alpha-cypermethrin 30mg/m2 and DDT 4.0%.\r\n', u'Results: Three main vectors of Dengue fever, Japanese Encephalic and Filariasis mosquitoes were found in a very high density in some areas of Ha Noi City. Aedes aegypti mosquitoes in the districts of Thanh Xuan (TX), Dong Da (DD) and Hoang Mai (HM) was found highly resistant to permethrin, alpha - cypermethrin, lambda - cyhalothrin and DDT with the mortalities rates of 51 % (TX) and 59%(DD); 70%(HM); 90%, 82% and 96%; 74%, 54% and 59%; 1 %, 8% and 32%, respectively. \r\n', u'Culex tritaenyorhynchus was found also highly resistant to permethrin with the mortality rate of 29%, alphacypermethrin - 18%, lambdacyhalothrin - only 3% and DDT x36%. Culex quinquefasciatus was found also highly resistant to those insecticides. \r\n', u'Conclusion: Other insecticides should choose to replace the chemicals used in this experiment to control, kill mosquitoes when epidemics occur.\r\n', u'
Insecticides ; resistance ; Aedes Aegypti ; Culex quinquefasciatus ; culex tritaenyorhynchus

Background: Human Heparansulphate interacting protein (hHip) has been shown to participate in biological processes of cells. Several studies indicated that hHip transcript is up regulated in several of cancer tissues including those of thyroid, colon, breast and prostate. Antibody against hHIP is necessary for methods to evaluate protein level of HIP in cancer tissues. Objectives:The aims of study was to induce anti hHIP antibody in rabbit and purify and conserve purified anti hHIP antibody. Subjects and method: The study included 9 adult and healthy rabbits with the weight 2 - 2.5kg. Immunization hHIP peptide-KLH in rabbit. Purify anti hHIP antibody using affinity chromatography. Results: The results shown synthesize hHIP peptide and conjugate it with carrier protein. Sensitive rabbit better meet with hHIP-KLH antibody. The Ig concentration obtained in sensitive rabbit was rather high and equal. Immunization hHIP-KLH successfully in rabbit. Obtainment valuable amount of anti hHIP antibody. Conclusion: Successfully induce and purify anti hHIP antibody from rabbit. Establish a standard protocol for polyclonal antibody against small peptide in rabbit.\r\n', u'\r\n', u'
Carrier Proteins/ administration & ; dosage ; chemistry ; Rabbits

Background: Suicide continues to be a leading cause of death worldwide. Recently, more than 45,000 children in the age group of 10 to 19 years died by suicide, making it the second leading cause of death in the age group of 15 to 19, surpassed only by traffic accidents, tuberculosis, and fighting. Objective: To determine the prevalence of suicidal ideation among adolescents whose parents are separated/divorced; and to explore the factors associated with suicidal ideation and describe adverse experiences among adolescents. Methods: A cross-sectional descriptive study was conducted in 309 adolescents with separated/separated parents in Hue City. Data was collected through direct interviews using a structured questionnaire. Suicidal ideation was defined as the presence of thoughts or plans related to suicide within the last 12 months. Multivariate logistic regression was applied to identify factors associated with suicidal ideation in adolescents with separated/ separated parents. Results: The study found that 15.5% (95% CI:11.7 - 20.1) of adolescents with separated /separated parents reported experiencing suicidal thoughts, in which men accounted 8.4% (95% CI:5.6 – 11.2) and women accounted 7.1% (95% CI:4.5 - 10.6). Several factors were identified as increasing the risk of suicidal ideation, including alcohol use (OR = 3.24; 95% CI:1.42 - 7.42), hyperactivity/inattention (OR = 4.96; 95% CI:1.58 - 15.605), and a poor quality of family relationships (OR = 4.82; 95% CI:1.26 - 18.50). On the contrary, certain factors were found to reduce the risk, including being in the 14-15 age group of 14-15 (OR = 0.26; 95% CI:0.10 - 0.69) and participating in physical activity (OR = 0.44; 95% CI:0.21 – 0.94). Conclusions: The research highlights a significant percentage of adolescents with separated / divided parents who experience suicidal ideation. Therefore, it is imperative for families, schools, and society to develop comprehensive strategies to monitor and address various risky behaviours among students simultaneously.