Abstracts Traditional drug Lider-5 traditional medicine, rheumatism and arthritis disease which has been used experimental animals we created arthritis disease antioxidant system of the Lider-5 studied the impact of the loss. As a result of the research that you created via a test animal collagen II arthritis pathological articular tissues and blood plasma contained malondialidyegid In addition to reducing the antioxidant Glutathione is provided a system of the body, including supyeroksidismutaza enzymes to increase the amount of influence. As you can see in the traditional drug Lider-5 disorders antioxidant system, loss prevention, and treatment is effective, the effects of its ingredients contained Tinospora sinensis, Gentiana decumbens, such as antioxidant effect might be associated with key plant attendant substances.

Background. This study is to determine mode of metabolism on triple collaboration bridges of traditional medicine, modern medicine and NCM.Goal. To determine membrane redoxy potentials three line involves important regulation factors on mode of metabolism which relationship connected with rlung, mkris, and badgan symbolic code.Materials and Methods. Only 81 healthy individuals were involved in the study. Proton leak was determined by quantity rate of MDA in cell membrane and membrane resistance, proton conductance was determined by serum and urine oxidase activity.Results. The table 1 shows quantity rate of membrane resistance was decreased 1.08-1.52 fold, HDL content was decreased, and however LDL was increased. This result is to manifest low proton leak which means this type is likely belonged to badgan symbolic code with qualities cold fatty, earth, water. The table 2 shows serum and urine oxidize activity 2.22-6.1 fold was increased, HDL content was increased; UCP-3 gene activity relatively was increased. This result is to manifest highproton conductance which means this type is likely belonged to mkris symbolic code with qualities hot fatty, fire.Conclusions:1. Individuals with high proton leak and slow proton conductance had serum and urine oxidize activity were weak, therefore there are visceral and subcutaneous fat were low.2. Individuals with medium proton leak and high proton conductance had serum and urine oxidize activity were high, therefore there are visceral was low and subcutaneous fat was high.3. Individuals with weak proton leak and medium proton conductance had serum and urine oxidize activity were medium, therefore there are visceral was high and subcutaneous fat was low.

Considering the fact that it is becoming important to broaden the studies of herbal medicine with few side effects which can treat acute and chronic inflammations of the liver and biliary tract, liver cirrhosis and can prevent from transformation into cancer, we chose Saxifraga hirculus L as our study objective which has been used in liver and biliary disease treatments of Tibetan – Mongolian traditional medicine for many years. This study aims to study one of the species of the family Saxifrgaceaca, Saxifraga hirculus L’s liverprotective activity and effect on the liver and biliary tract diseases resulted from bile stasis, which frequently comes as an ingredient of compound prescription of the traditional medicine. 01/01 Study methods: Experimental pathological liver disease model was developed in total of 520 white rats and 80 white mice in 2 ways, Saxifraga hirculus L’s bile-forming and detoxifying function and effects on the detergent effect of bile, necrotic liver cell lysis and stability of lung cell surfactant were compared with the standard drugs such as Legalon and Cholasos and double proven by biochemical, pathohistologi and histochemical studies. 70% alcohol extract was prepared from the selected Saxifraga hirculus L, and dried and decocted extract was obtained from this and given to the experimental animals by oral intragastric tube in a dose of 100mg/kg. As the result of pathohistologi and histochemical study, necrosis and necrobiosis of liver cells were dominantly observed in CCl4 induced “toxic inflammation”, cholestatic syndrome and dysfunction of detoxication action were observed in Tetracycline Hydrochlorideinduced(druginduced)toxic inflammation in the experimental animals. The Saxifraga hirculus L’s most potent dose to treat and protect liver was estimated as 100mg/kg and this was 31 times lower than its LD50. In our study,Saxifraga hirculus L has possessed an anti-inflammatory effect on each stages of inflammation which is alteration, exudation and proliferation. In other hand, Saxifraga hirculus L increased the biliary secretion and excretion speed, protected from toxic detergent effect of bile acid, improved detoxication effect of the liver, protected from the necrotic cell lysis, showed an antioxidant and membrane stabilizing activity and these actions were compared with the standard medicines such as Legalon and Cholasos which in some cases, above mentioned effects were higher than these drugs. The hepatoprotective action of Saxifraga hirculus L may have been related to its contents such as compound flavonoids of the quercetin group, aglycone and bitter hyperoside. Also in the case of drug induced toxic hepatitis, Saxifraga hirculus L increased the amount of glycogen and nuclear DNA in the liver tissue by 22-24.5% compared to the control group and was proven by histochemical study.

Background: The immune response has become the basic use of today’s medical and biological sciences understanding, definition and realization when first introduced into the cognitive level with new theory of nine step closed cycle of protons related to membrane potentials three linear and s-NCM. Medicinal preparations for the immune system derived from traditional herbs have rarely been used therefore, we aim to determine the boost-reduction of immune response with preparation Antischemin with ingredients of Gingko biloba, Astragalus membranaceus and Scutellaria baicalensis. Materials and Methods: This study was carried out on the innovation-research bio-modeling laboratory of the “New Medicine Medical University”. Sheep blood was taken in heparin tube and centrifuged for 10min with speed of 2000rpm and plasma was isolated. Blood components were washed with physiological solution 3-4 times and 10% blood red cell suspension was prepared. Prepared 0.2ml of 10% sheep red cell suspension was injected to mice tail vein to create immune response model (N.K. Jerne and Nordin (1963)). IL-4 (pg), antibody titre SRBC-IgM(ng/mL), and hemagglutinin titre (%) was measured with ELISA kit and compared with control group. Comparing group animals used Salimon 1 ml/kg, Dexamethasone 1 mg/kg orally. The study was conducted in accordance with the approval of Ethics Review Committee of Ministry of Health (November 02, 2018 and approval number 10, №1). Result : In addition, comparing mice which used Antischemin 100 mg/kg orally with sheep blood injected group at day 5, IL-4 level increased by 12.11%, but conclution of antibodies against sheep blood-IgM concentration decreased by 28.7%, and hemagglutinin titre decreased by 27.5%, meaning we observed that the preparation can suppress immune response. Сonclusions Antischemin preparation meaning observed can suppress immune response.

BACKGROUND: According to the World Health Organization (WHO), 10-15% of couples of reproductive age have infertility. According to researcher D. Sukhe (1999), hormonal infertility in the reproductive age of women was 33.6%, which was a large part of the cause of infertility. In recent years, the number of cases of endocrine disorders, including malignancy and sexually transmitted infections, has been increasing year by year. According to WHO reports, thyroid disorders have a prevalence of 49.3% for active reproductive age (30-50). According to the report of the Health Development Center in 2016, since the thyroid disorders are the second most common disease in endocrine gland disease, our study found that the infertility in reproductive age of women can be substantial due to the loss of thyroid gland. METHODS: The study was carried out by the couple of 20-45 year-olds and modeled as an analytical study model. The questionnaire was used for the couple’s interviews and some of the measurement of body and serum use of TOSOH Corporation AIA-360, Tokyo, Japan. On the serum, anti-TPO and аnti-TG carbohydrates are identified by the Cobas e-411 analyzer under the manufacturer’s accompanying protocol. RESULTS: 76.7% of women were diagnosed with infertility euthyroid, 0.7% hyperthyroidism, 22.6% hypothyroidism (3.8% with overt hypothyroidism and 18.8% subclinical hypothyroidism). Prevalence of TAI, in 6.7% isolated positive anti-Tg were found, and 14.3% had isolated positive TPO, In 3.7% of cases, both types of autoantibodies were present. We analysed binary logistic regression for anti-TPO and anti-TG autoantibody in the positive and negative group in past obstetrics history, evidence of positive of anti-TPO and anti-Tg was increased risk of miscarriage 2.2 times (OR = 2.2, p <0.01). CONCLUSIONS: Women with disorders in our study have high percentage of subclinical hypothyroidism and have higher rate of thyroid autobodies in serum which may be a problem for women with infertility and pregnancy complications due to the loss of thyroid gland. There is a need to develop a principle of recovery and treatment.

Background: Valerian (Valeriana officinalis) and Hops (Humulus lupus) are famous herbs in throughout world. Both medicinal herbs have been used for insomnia in European, Asian traditional medicine for centuries. Valmedin consists from mixed herb extracts of Valerian and Hops. It possess antidepressant, anti-anxiety effect, and improves sleep efficiency. Aim : The aim of this study was to evaluate quality control parameters for standardization of Valmedin pellet and assess toxicity of Valmedin pellet. Methods: In the present study pellets was prepared by extrusion-spheronization technique. The pellet size was determined by sieve analysis using sonic separator. The extract of Valmedin formulation was prepared in pellet (dragee). Valmedin pellet was subjected to analysis for physiochemical parameters, phytochemical studies, heavy metals and quality parameters according to Mongolian pharmacopoeia (2002,). The lethal dose at 50% (LD50) was calculated according to Prozorovskii. In the test chronic toxicity was used two doses of the concentrated extract of Valmedin (0.25; 0.5 g/kg) and assessed parameters (ALT, ALP, AST, bilirubin, total protein, TNFα, IL-6). Result: First of all, the quality of biological active components and physiochemical parameters of individual herbs including Valerian, Hops and Metha L have been fulfill the basic requirements of standardization herbal medicines. Moisture content of Valmedin pellets was 1.6 ±0.01%. Total tannin and valerenic acid content found to be 0.051±0.05, 0.71±0.02% for a pellet. Levels of heavy metals was within the permissible range of standard.
In the acute toxicity study, the LD50 value of the concentrated extract from Valmedin was 1.56 g/kg (i.p). No significant changes in body weights of treated animals. In the hematological and biochemical analyses of Valmedin in dose of 250 mg/kg has shown no significant change in the levels of ALT, AST, ALP and levels of RBC and WRC counts in blood serum. Valmedin at dose of 500 mg/kg after chronic injection lower sign levels of ALT, ALP enzymes, and total protein compared with the control group I. It increases significantly levels of AST enzyme, glucose in blood serum. By hematological analyses, Valmedin in dose of 500 mg/kg elicited decrease in the level of hematological parameters such as white blood cells and red blood cells count in whole blood. Likewise, treatment of Valmedin in doses of 250 and 500 mg/kg did not induce any change in the levels of TNF and IL-6 cytokines. Conclusion Pellets of Valmedin containing Valerian, Hops, and Metha were successfully made achieving degrees of quality, physical stability compatible with the requirements for standardization and formulation.

The main mechanism of sand sauna therapy is lie into heated sand baths for adults 8 to 20 min and for child 7 to 15 min long, and it is performed twice a day (morning and afternoon) for 2 or 3 weeks once a year. Particularly, the sanatorium offers a type of balneotherapy through the application of thermally warmed sand and a combination of healthy micro-bioclimate, purity of the natural environment, and the presence of fresh and healthy local foods to treat a wide range of medical conditions. The sand sauna treatment most likely affect the body by means of the sand’s high temperature and the presence of chemical elements those are biologically important. One of the well-experienced health resorts is the Tavan Els kidney sanatorium. The Tavan Els sanatorium treats patients with the following signs: fatigue, shortness of breath, bone and back pain, swelling of the legs, high blood pressure (over 130/80mmHg), infrequent urination, proteinuria, and blood in the urine. Their goal is to delay progression of chronic kidney diseases prevent or slow additional damage to the patient’s kidneys, improve blood circulation, decrease the use of medicines, and educate patients about alternative therapies. Our study investigated effects of sand sauna treatment more considering some laboratory findings. There conducted totally 720 patients where 429 inpatients and 291 outpatients. This study selected 150 patients with chronic glomerulonephritis symptoms of swelling 31%, blood in urine 9%, high blood pressure (Over 130/80 mmHg) 10% and mixed conditions 50%. After the 10 days treatment, the proteinuria was decreased 52.6%, and blood in urine decreased 16, 6% from the total number of patients. Hypertension was decreased 35% of patients from 10 to 20mmHg and 45% of patients did not changed. A swelling was cleared 51%, 38% decreased and 3% did not change. 66% of patients down the weight loss about 2-4kg, and 34% of patients did not change. We observed typical changes during 15-20 minutes bathing, heart rate increased from 65±1.3 bpm at rest to 82±2.4 bpm in 45°C hot sand bath. Blood pressure increased from 132±4.0/82±2.2 mmHg at rest to 139±1.4/81±1.0 at the bathing. Body temperature was increased 1- 2°C during bathing. Because of the equipment limits, we could not check the urinary sediment, and serum creatinine. Sand sauna treatment appears to be an effective and useful method to treat financial and emotional burdens problems of patients with chronic kidney disease problems that especially for the symptoms of high blood pressure, cause of swelling, proteinuria, infrequent urine and blood in urine as well as improvements of well-being.

Background: Gout is a chronic inflammatory disorder stemming from metabolic dysfunction, characterized by the accumulation of monosodium urate crystals in joints and soft tissues due to prolonged hyperuricemia. Clinically, it often manifests with abrupt onset of intense joint pain, erythema, swelling, and tenderness, particularly in the small joints of the lower extremities such as the first metatarsophalangeal joint, ankles, and knees. In its chronic form, gout can lead to the development of tophi—firm nodular deposits of urate crystals— resulting in joint deformities, limited mobility, and systemic complications affecting the renal and cardiovascular systems. This case report describes the integrative management of a 68-year-old male patient with a long-standing history of chronic tophaceous gout, presenting with acute exacerbation involving multiple joints and extensive tophaceous deposits. The patient underwent a course of traditional Mongolian medical treatment, incorporating bloodletting therapy (hanuur), soaking therapy (devteeleg zasal), and phytotherapeutic remedies. Conclusion The treatment yielded notable clinical improvements: cupping therapy facilitated the reduction of joint inflammation and edema, alleviated pain, and enhanced peripheral circulation during the acute phase. Soaking therapy using medicated decoctions was effective in softening and reducing the size of tophi, promoting wound drying, restoring joint mobility, and improving overall functional capacity. The patient reported a marked enhancement in quality of life following the integrative treatment course. This case highlights the potential of traditional Mongolian medical interventions as adjunctive or alternative therapeutic strategies for managing severe gout, particularly in cases refractory to conventional pharmacological treatment. Further clinical research and controlled studies are warranted to validate these findings and to explore the broader application of traditional therapies in gout management.

Introduction: Rhodiola rosea L. (R.rosea) is a popular plant in traditional medicine of the Nordic countries, Eastern Europe, and Asia. R.rosea plants are successfully cultivated in Mongolia. The Botanical Garden of Medicinal Plants under the “Monos” Group started to cultivate R. rosea since May 2015. Objective: The aim of this research was to study the salidroside contents of R.rosea collected from Zavkhan and Khuvsgul province, Mongolia, and cultivated in the Botanical Garden of Medicinal Plants, Drug research Institute, Monos group. Material and Methods: The underground parts of wild roseroot plants were collected from April to May 2020 from Jargalant soum, Khuvsgul province, and Nomrog soum, Zavkhan province, 3-years and 4-years-old cultivated R.rosea gathered from the Botanical Garden of Medicinal Plants in April 2020. For comparison, 4-year-old Rhodiola grenulata (R. grenulata) was ordered from Shanxi Zhendong Genuine Medicinal Materials Development Co., Ltd, China, and used for the study. The quantity of the salidroside constituents of the underground parts were compared and the sourcing of roseroot raw material was evaluated. Chemical analysis of roots and rhizome of R. Rosea namely the appearance, identification, moisture, organic impurities, mineral impurities, residue on ignition, water-soluble extractives, fresh weight of roots, and salidroside content were determined according to the National Pharmacopoeia of Mongolia (NPhM) 2011. Microbiological analysis was performed in accordance with the requirements of grade 3b specified in Annex 1 of the Order No. A / 219 of the Minister of Health dated May 30, 2017 to determine the degree of microbiological purity in medicinal products of roots and rhizome raw materials. Result: The content of salidroside, the main biologically active substance of R.rosea plant, was 1.57% in samples collected from Zavkhan province, 1.45% in samples collected from Khuvsgul province, 1.7% in samples grown in China and 0.25% for 3-years-old samples and 1.89% for 4-years-old samples grown in the Botanical Garden of Medicinal Plants, Monos group, Mongolia. In addition, these raw materials meet the general requirements for plant raw materials and microbiological parameters. Conclusion Samples of underground parts of R.rosea cultivated for 4 years in the Botanical Garden of Medicinal Plants have the highest content (1.89%) of the salidrosde. Therefore, it is suggested that the roots and rhizomes of R.rosea planted in the future can be standardized and used as a raw materials for medicines.