1.Transperineal template-guided prostate saturation biopsies in men with suspicion of prostate cancer: a pilot study from Pakistan
Khalid MEHMOOD ; Muhammed MUBARAK ; Murli DHAR ; Mansoor RAFI ; Janette KINSELLA
The Malaysian Journal of Pathology 2017;39(3):285-288
Traditionally, transrectal ultrasound (TRUS)-guided biopsies are done for the diagnosis of prostatecancer (PCa) in Pakistan. The transperineal template-guided saturation biopsy (TTSB) approach hasbeen recently introduced in Pakistan and we share diagnostic yields and pathological findings ofspecimens taken for PCa diagnosis in men with elevated serum total prostate specific antigen (PSA)and negative TRUS-guided prostate biopsies. In all, 16 patients investigated at the Department ofUrology, Sindh Institute of Urology and Transplantation (SIUT), underwent TTSB. The mean ageof patients was 67.8 ± 8.8 (range: 55 - 84) years. The median PSA was 9.5 (IQR: 7.9 - 19.8) ng/ml. The duration of symptoms before biopsy ranged from 1 month to 144 months. The prostatewas enlarged with mean weight of 73.5 ± 55.5 g. Histopathology revealed PCa in 5 of 16 (31.2%)cases. The Gleason score was 6 (3+3), 7 (3+4) and 8 (4+4) in 1 case each (6.3%) and 10 (5+5) in 2cases (12.5%). At least two cores were positive in all positive cases. None of the patients requiredantibiotics post-procedure. In conclusion, the TTSB technique is a promising option for patients withelevated PSA level and negative transrectal prostate biopsies for the detection of PCa in our settin
2.Tibial dyschondroplasia is closely related to suppression of expression of hypoxia-inducible factors 1α, 2α, and 3α in chickens
Shucheng HUANG ; Mujeeb U REHMAN ; Gang QIU ; Houqiang LUO ; Muhammad K IQBAL ; Hui ZHANG ; Khalid MEHMOOD ; Jiakui LI
Journal of Veterinary Science 2018;19(1):107-115
Tibial dyschondroplasia (TD) cases has not been reported in Tibetan chickens (TBCs), but it is commonly seen in commercial broilers characterized by lameness. The underlying mechanism remains unclear. Hypoxia-inducible factors (HIFs) are important regulators of cellular adaptation to hypoxic conditions. In this study, we investigated the role of HIF-1α,
Anoxia
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Blotting, Western
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Chickens
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Growth Plate
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Osteochondrodysplasias
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Poultry
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Reverse Transcriptase Polymerase Chain Reaction
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RNA
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Thiram