Objective Explore the difference of water channel protein 4 (AQP4) 's expression level in different grade glioma tissues.Methods select 57 cases of paraffin embedding glioma tissues and 10 cases of normal brain tissue in our hospital from 2008 to 2012,use immunohistochemical method to test the level of AQP4 and do statistical tests.Results The AQP4 expression level in high grade glioma tumor tissues is higher than in the low grade glioma and normal brain tissue (P ＜ 0.05),and there is no statistical significance about the difference AQP4 expression levels between low grade glioma tumor tissue and normal brain tissue (P ＞ 0.05).Conclusions AQP4 expression level in glioma is closely related to the tumor pathological level.