Mandibular buccal bifurcation cyst is a rare inflammatory odontogenic cyst. We reported two cases who complained of painful swelling of extraoral soft tissue. Intraoral examination revealed the partially erupted mandibular first molar. Cone beam computed tomography showed a well-defined cystic lesion surrounding the first molar. Histopathologic images showed the cyst wall was infiltrated by a large number of plasma cells, neutrophils and eosinophils, and lined with a thin layer of non-keratinized stratified squamous epithelium. Finally, the two patients were diagnosed as mandibular buccal bifurcation cyst and treated with cyst enucleation and curettage.

To study the biomechanical behaviors of the cells, reliable fluid shear stress loading system is needed. Compared to the traditional parallel plate flow chamber (PPFC) system, a rocking system presented by Zhou offers some advantages such as easier operation, lower cost and higher quantity of pocessing. But the feasibility of it has not been practically studied. To investigate the feasibility whether the rocking system can be used to apply quantified fluid shear stress loading, primary osteoblasts of mouse were loaded with fluid shear stress based on rocking system and traditional PPFC system, respectively. Another group of cells was unloaded as control. The cytoskeleton was observed with laser scanning confocal microscope (LSM) and average fluorescence of F-actin was recorded. Cell cycle was also measured by flow cytometry and percentage of S-phase cells was recorded. The result showed that average fluorescence of F-actin was enhanced after rocking system loading (46.8 +/- 4.5) compared to the control (20.4 +/- 1 8) and the percentage of S-phase cells was increased (10.6 +/- 1.04) after rocking system loading as well (which was 4.1 +/- 0.54 in control group). Furthermore, the fluid shear stress generated by rocking system could induce more significant biological effects compared to PPFC system. This study demonstrated that fluid shear stress generated by rocking system could induce biological effects of osteoblasts, and it could simulate the micro environment of cells in vioe better than PPFC. Rocking system is a convenient and feasible method for fluid shear stress loading.
Animals ; Animals, Newborn ; Cell Proliferation ; Cells, Cultured ; Cytoskeleton ; ultrastructure ; Feasibility Studies ; Mice ; Mice, Inbred BALB C ; Osteoblasts ; cytology ; Shear Strength ; Skull ; chemistry ; Stress, Mechanical

Tooth extraction is a common and widely employed therapeutic procedure in oral and maxillofacial surgery.Minimally invasive tooth extraction can reduce both physical and psychological trauma to the patients,and is widely recommended as a first-line clinical treatment.But currently no guidelines or consensus has been available to provide a systematic introduction of minimally invasive tooth extraction to guide the clinical practices.To address this issue,this consensus,based on a comprehensive literature review and clinical experiences of experts,systematically summarizes the indications,target patients,and contraindications of minimally invasive tooth extraction,the overall workflow of this procedure(preoperative preparation,surgical steps,postoperative management,postoperative instructions,medications,and follow-up),and its common postoperative complications to provide a comprehensive guidance for clinical application of this technique.

Tooth extraction is a common and widely employed therapeutic procedure in oral and maxillofacial surgery.Minimally invasive tooth extraction can reduce both physical and psychological trauma to the patients,and is widely recommended as a first-line clinical treatment.But currently no guidelines or consensus has been available to provide a systematic introduction of minimally invasive tooth extraction to guide the clinical practices.To address this issue,this consensus,based on a comprehensive literature review and clinical experiences of experts,systematically summarizes the indications,target patients,and contraindications of minimally invasive tooth extraction,the overall workflow of this procedure(preoperative preparation,surgical steps,postoperative management,postoperative instructions,medications,and follow-up),and its common postoperative complications to provide a comprehensive guidance for clinical application of this technique.

Oral squamous cell carcinoma (OSCC) is the most common oral cancer. Previous studies have found significantly high miR-155 expression in OSCC. However, the mechanism by which miR-155 plays a role in OSCC oncogenesis is not yet clear. This article reviews the function of the relationship between miR-155 and tumors and the potential role of miR-155 in the development of OSCC. A literature review showed that mir-155, as a small carcinogenic RNA, can inhibit CDC73, BCL6, P27Kip1 and other target genes that play a role in cancer inhibition; promote the proliferation, migration and invasion of OSCC cells; and inhibit apoptosis. miR-155 can also be combined with biological factors (Epstein-Barr virus, human papillomavirus) to promote the development of OSCC.

Objective: Exploring the effect of three-flap paltoplasty in preventing anterior palatal fistula for patients whose anterior fissures measured more than 0.5 cm. Methods: 12 patients aged 18-24 months with unilateral complete cleft palate were selected for the implementation of three-flap paltoplasty for cleft palate repair. Briefly, three-flap paltoplasty is based on the traditional two-flap paltoplasty method and involves the creation of a mucoperiosteal flap A in the contralateral palate in front of the fissure margin that is approximately half the size of the anterior palate. The flap A was sutured to the edge of the contralateral nasal mucosa, and the mucoperior flap of both sides of the loose fissure was sutured in layers, and the suture was removed two weeks after surgery. The recovery of cleft palate was observed. Results : All patients were followed up for 3 months, and 12 patients underwent successful repairs with no fistula and other complications. Conclusion Three-flap paltoplasty is an effective method of preventing anterior palatal fistula.