AIM: To investigate the clinical use and effect of a removable obturator made of elastic bionic materials in treating food impaction.METHODS: The removable obturator made of elastic denture materials were applied to 27 patients who suffered either mixed or horizontal food impaction and were admitted in Guilin Stomatological Hospital from October 2006 to April 2007. At weeks 4 and 12 following treatment, all patients were surveyed by questionnaires to investigate wearing comforts, and the histocompatibility of the obturator was simultaneously evaluated. Periodontal indexes were recorded before treatment, at weeks 4 and 12 after treatment. X-ray radiography was also performed before treatment and 12 weeks after treatment to compare alveolar condition.RESULTS: All the 27 patients were involved in the result analysis. They were all satisfied with the obturator after twelve weeks treatment, and the questionnaire showed 96.3%-100% satisfactions from the patients. The measuring results of periodontal indexes at different periods of the treatment showed no statistical significance. There were no remarkable changes in the tooth mobility and X-ray image analysis before treatment and 12 weeks after treatment.CONCLUSION: With good histocompatibility, the removable obturator made of elastic denture materials has been proved to be well histocompatible, acceptable and effective for the patients who suffer from food impaction in the short term.

Objective:To observe the effect of overdose fluoride on the expression of bFGF in rat's dental pulps.Methods:NaF at 20 mg/(kg?d) was given to each of 10 Wister rats by stomach perfusion for 8 weeks, another 10 rats were given the same volume of distilled water as the controls.After treatment,HE staining was used to observe the morphology of the teeth, immunohistochemical staining was adopted for study the expression of bFGF in the dental pulps of the rats.Results:The enamel layer and dentin layer of the incisors in NaF treated rats were thinner than those in the controls,the interglobular dentin was increased and the expression of bFGF in dental pulp and inner dentin was inhibited in the NaF treated rats(P

Objective To conduct a descriptive epidemiological study on thyroid cancer incidence in the urbanarea of Tianjin during 2002 - 2006 and to analyze the secular trend of incidences based on previous data of 26 years and those from Shanghai and the United States. Methods Annual percent change (APC%) of thyroid cancer incidence and the rank of all-site cancer profiles were estimated. The secular trend of different histological incidences and the proportion changes were analyzed. Results The incidence of female thyroid cancer was 6. 55/100000 in 2006, ranking the eighth in the female cancer profile. The incidence of thyroid cancer showed an increased secular trend in recent 26 years, especially a 5.7-fold increase in the papillary thyroid cancer (PTC). The incidence for female increased by 267% in 2006 compared to that in 1981, APC% was 3. 1%. The variation ranked second among all the female tumors. The incidences of thyroid cancer in Shanghai and the United States also elevated with time.Conclusions Thyroid cancer has been one of female common tumors from a once less common tumor,especially the proportion of PTC is expanding. It suggests that further study on the risk factors and preventive efforts should be made.

Objective To investigate the trends in incidence and mortality of esophageal cancer from 1981 to 2002 in Tianjin,China and to predict future trends and facilitate future prevention.Methods All new cancer cases registered in the Tianjin Cancer Registry between 1981 and 2002 were reviewed.Yearly age-specific,crude and age-adjusted incidence rates were calculated for males and females.Secular trends of incidence and mortality were indicated by the estimated annual percentage change(EAPC).Results A total of 8206 cases of esophageal cancer were reported in Tianjin form 1981 to 2002,with 5533 males and before 45 years,but increased rapidly after 45 years.The median age of esophageal cancer cases was 69 years,with quartile range from 62 to 75 years.The peak for age specific incidence in females was 75-80 years,which was earlier than that in males(aged 80-85).But recent data indicated that the peak age in females had increased and became similar to that of the males.The crude and age-standardized incidence rates(ASIR)of esophageal cancer decreased in last 22 years.For male and female,crude incidence decreased by 2.22% and 3.56% per year and ASIR decreased by 5.18% and 6.56% per year.The mortality of esophageal cancer decreased in the last 22 years.Conclusion The incidence of esophageal cancer decreased in the last 22 years in Tianjin,but there is little overall improvement in survival.

Objective In this study ,we constructed a series of recombinant plasmids carriers expressing shRNA targeting hTERT and Bi‐1 gene .These recombinant plasmids carriers were transfected into CNE‐2Z cell lines using Lip and continuously in‐duced the expression of shRNAs .Furthermore ,the shRNAs caused the degradation of mRNAs homologous in sequence with the target genes ,which lead to a sequence‐specific gene silencing .Methods The CNE‐2Z cells was divided into untreated group ,pEG‐FP‐N1 group and pEGFP‐N1/Lip group .Flow cytometry(FCM ) was applied to determine the transfection efficiency .The changes of hTERT and Bi‐1 gene expression were detected by Real‐time RT‐PCR and Western blotting .Results The best transfection effi‐ciency between plasmid and Lip was 2 .5 μg plasmid and 6 .25μL Lip .Conclusion We constructed several shRNA recombinant eu‐karyotic expression plasmids successfully .The recombinant plasmid can inhibit the expression of hTERT and Bi‐1 gene specifically and effectively .

OBJECTIVE:To explore the effects of extracts from Siegesbeckiae herba (HS) on doxorubicin (DOX)-induced chronic myocardial injury in mice. METHODS:48 mice were randomly divided into 3 groups. Mice in blank control(Con)group received distilled water once every day,ig,and normal saline(2 mL/100 g)once every day,ip,for 8 weeks;mice in DOX mice received distilled water(2 mL/100 g)once every day,ig,and DOX(3 mg/kg)once every week,ip,for 8 weeks;mice in DOX+HS group received HS(340 mg/kg)once every day,ig,and DOX(3 mg/kg)once every week,ip,for 8 weeks. After administra-tion,body mass,heart coefficient,cardiac function changes,serum biochemical index levels [alanine aminotransferase(ALT),as-partate aminotransferase (AST), creatine phosphokinase (CK), creatine kinase isoenzyme (CK-MB), lactate dehydrogenase (LDH)and total cholesterol(TC)] of mice were determined. RESULTS:Compared with Con group,body mass of mice in DOX group was decreased(P<0.01);heart coefficient was increased(P<0.05);heart rate slowed down,R-wave was increased(P<0.05 or P<0.01);serum biochemical indexed were increased,there was significant difference in AST(P<0.01). Compared with DOX group,heart coefficient of mice in DOX+HS group was decreased (P<0.01);heart rate was increased (P<0.01);serum biochemical indexes were decreased,there was significant differences in CK,LDH,TC(P<0.01). CONCLUSIONS:HS has cer-tain protective effects on DOX-induced chronic myocardial injury in mice.

Objective To study the serum peptide fingerprint using matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) technology,and find the different peaks with potential significance and establish the diagnosis model of Staphylococcus aureus and Escherichia coli bloodstream infection.Methods To establish ICR mice model of S.aureus and E.coli bloodstream infections,and collect serum samples.The serum samples were purified by weak cation exchange beads,the serum peptide fingerprint was recognized by using MALDI-TOF MS and BioExplorer software between infections group and normal control group.Results Compared with the normal control group,6 peptides were up-regulated,7 peptides downregulated and 8 peptides up-regulated first and then down-regulated in S.aureus infection group;And 5 peptides down-regulated,4 peptides down-regulated first and then up-regulated,and 8 peptides up-regulated first and then down-regulated in E.coli infection group.Conclusion MALDI-TOF MS combined with BioExplorer software may be used as a tool to study the serum peptides of S.aureus and E.coli bloodstream infection,effectively find significant peptides for establishing a diagnosis model of these two bacterial infections,and has a certain value for the diagnosis of bacterial bloodstream infection.

OBJECTIVETo observe the trend in the change of incidence and mortality of female breast cancer in Tianjin and evaluate its effect of prevention.

METHODSMethod of descriptive epidemiology was used to study the epidemic situation of female breast cancer in Tianjin.

RESULTSThe incidence of breast cancer in Tianjin had increased by 39.7% from 1981 to 1997, as compared with the other cities in China. Especially, compared with the developed countries the world over, the incidence of breast cancer in Tianjin is much lower. The mortality rate of breast cancer had lowered by 31.0% from 1981 to 1997 and the 3-year and 5-year survival rates increased to various degrees.

CONCLUSIONEarly detection and diagnosis of breast cancer are very important to both increase of survival and lowering of mortality of breast cancer. Preventive efforts should be made to the high risk people of breast cancer.

Adult ; Aged ; Aged, 80 and over ; Breast Neoplasms ; epidemiology ; mortality ; China ; epidemiology ; Female ; Humans ; Incidence ; Middle Aged ; Survival Rate

Objective To investigate the clinical effectiveness of extended care on coronary intervention therapy in patients with acute myocardial infarction. Methods 90 patients with acute myocardial infarction who had been through coronary intervention therapy successfully in our hospital from June 2014 to April 2015 were divided into study group and control group with 45 patients in each according random number table. Patients in control group were treated with routine nursing care intervention while patients in study group were treated with additional extended care. Clinical effectiveness of nursing care in two groups were observed. Results After the intervention of extended care, there were 2 cases with nonfatal myocardial infarction (4. 4％), 3 patients undergoing revascularization for a second time (6. 7％) and one death (2. 2％) in study group, which were significantly fewer than those in control group, the difference was statistically significant(P＜0. 05). 30 cases were satisfied with extended care and 11 cases were somewhat satisfied in study group after intervention. The satisfactory rate in study group was 91. 1％, which was significantly higher than those in control group (66. 7％), the difference was statistically significant (P＜0. 05). According to the results of follow-up visit, nursing compliance of diet, exercise, medication and review in patients of study group was better than that in control group, the differences were statistically significant(P＜0. 05). Conclusion Extended care on coronary intervention therapy in patients with acute myocardial infarction could reduce the incidence of adverse reaction, improve the satisfactory rate of nursing care and improve patients ' quality of life. It was worth promotion.

Objective To investigate the expressions of leukaemia inhibitory factor (LIF) and regulated upon activation,normal T cell expressed and secreted factor (RANTES) in mice with bloodstream infection by 4 different single pathogen and provide research basis for the early diagnosis of bacteriogenous bloodstream infection.Methods CD-1 (ICR,Institute of Cancer Research) mouse models of bloodstream infection with the standard strains of Staphylococcus aureus (S.aureus),Enterococcus faecalis (E.faecalis),Escherichia coli (E.coli) and Klebsiella pneumonia(K,pneumoniae) were established.The serum samples were collected at the 0.5,1,3,6,12,24 and 48 hours after infection and the concentrations of LIF and RANTES in mouse serum of experimental groups and control were detected by Luminex liquid chip system.Results The median lethal dose (LD50) of S.aureus,E.faecalis,E.coli and K.pneumoniae were 8.1 × 108/mL,9.6 × 108/mL,8.1 × 108/mL and 1.1 × 109/mL,respectively.The concentration of serum LIF was significantly increased in 1 hour after infection.The peak concentrations of LIF in the four groups were (51.6±5.0),(73.2±20.8),(7.3 ±0.9)and (6.1 ± 1.2) pg/mL respectively,and the differences were statistically significant compared with the control group (P ＜ 0.01).The concentrations of RANTES in E.faecalis group,E.coli group and K.pneumoniae group were increased after infection for 1 hour and increased significantly after infection for 3 hours.The increased concentrations of RANTES in E.coli group and K.pneumoniae group were more than those in S.aureus group and E.faecalis group.The peak concentrations of RANTES in S.aureus group,E.faecalis group,E.coli group and K.pneumoniae group were (1 929.0-± 25.2),(1 218.1 ± 227.4),(55.7 ± 10.0) and (179.2 ± 9.2)pg/mL,and the differences were statistically significant compared with the control group (P ＜ 0.01).Conclusion The concentrations of LIF and RANTES increased obviously in 1 h after the bacteria entered bloodstream.After 2 days of infections,the levels of LIF and RANTES in E.coli group and K.pneumoniae group were significantly higher than those in S.aureus group and the E.faecalis group.Combined detections of LIF and RANTES may be of certain values to differentiate the infections caused by the pathogens between gram positive and gram negative bacteria.