OBJECTIVES: This study was aimed to investigate the protective effects of dexpanthenol (Dxp) on against cisplatin-induced ototoxicity. METHODS: To examine this effect, distortion product otoacoustic emissions (DPOAEs) measurements and serum levels of oxidative and antioxidant status (including malondialdehyde, superoxide dismutase, catalase, glutathione, glutathione peroxidase, total oxidant status, total antioxidant status, and oxidative stress index) were evaluated. Thirty-two adult female Wistar albino rats were randomly divided into 4 equal groups; control (K), cisplatin (C), cisplatin plus Dxp (CD), and Dxp (D). In all groups DPOAEs measurements, between 996 and 10,078 Hz as DPOAEs and input/output functions, were performed on days 0, 1th, 5th, and 12th. Prior to death, the last DPOAEs measurements and blood samples were taken. RESULTS: In the C group, statistically significant differences were detected at all frequencies between 0 and 5 days and 0 and 12 days measurements (P<0.05). Serum level of oxidant and antioxidant status were detected statistically significantly changed in this group versus K group (P<0.05). Contrary to the C group, in the CD group hearing ability was seen largely preserved at many frequencies and serum levels of all biochemical parameters were shifted toward normal values, similar to the K group. No significant differences were detected in the either D or K group's measurements. CONCLUSION: According to these results, Dxp may prevent cisplatin-induced ototoxicity.
Adult ; Animals ; Biomarkers ; Catalase ; Cisplatin* ; Female ; Glutathione ; Glutathione Peroxidase ; Hearing ; Humans ; Malondialdehyde ; Oxidative Stress ; Rats ; Reference Values ; Superoxide Dismutase

Objective: To explore the effects of Nigella sativa oil (NSO) on the histopathological and biochemical changes that inhaled formaldehyde (FA) induces on the testicular tissue of rats. Methods: Thirty three adult male rats were separated into five groups as follows: C, the control group; 4FA group which received FA for 4 weeks; 13FA group which was given FA for 13 weeks; 4FA+NSO group which was administered FA plus NSO for 4 weeks; 13FA+NSO group which was treated with FA plus NSO for 13 weeks. FA was administered through inhalation for 8 h 5 days a week at a dose of 5 ppm in a special glass cage, and NSO was administered orally 1 mL/kg once daily. Rats were decapitated at the end of the experiment and testicular tissue specimens were harvested for histopathologic and biochemical assessment. Results: Compared to the C group, reduction was observed in the number of intact tubules and in the mean germinative epithelium thickness of the FA groups. Significant increase was observed in the number of intact tubules with the long-term (13 weeks) administration of NSO together with FA. Reduced glutathione peroxidase activity was found and oxidative stress index values were measured higher in the 4FA and 13FA groups versus the C group (P<0.05). Moreover, total antioxidant status levels decreased only in the 4FA group (P<0.05) while only the 13FA group significantly increased malondialdehyde levels and reduced catalase activities in comparison with the C group. In the 13FA+NSO group, malondialdehyde levels decreased however glutathione peroxidase and catalase activities increased compared to the 13FA group. Differences measured in total antioxidant status levels were found to be statistically significant only between the 4FA and the 4FA+NSO groups. Conclusions: NSO as an antioxidant should be used for a longer term to achieve protective efficacy both histopathologically and biochemically in the testicular tissue.