The purpose of this study was to evaluate the relationship between proliferation and cell death during oral carcinogenesis. Syrian golden hamsters which were 3 month old and 90-120 gm-weight were used in this study. The 9, 10-dimethyl-1, 2-benzanthracene(DMBA) in a 0.5% solution in mineral oil was painted on the buccal pouch mucosa 3 times per week in the experimental group during 20 weeks. Control group was painted with mineral oil. In each control and experimental group of 6,8, 10, 12, 14, 16, 18,20 weeks, specimen were sectioned for immunohistochemical study with anti-Proliferating Cell Nuclear Antigen(PCNA), anti-transglutaminase transglutaminase and GST-pi were obtained by counting the positive cells to those antibodies. The following results were obtained. 1. Histopathologically, finding of epithelial dysplasia of the 6 and 8 weeks experimental group and carcinoma in situ in the 12 weeks and squamous cell carcinoma in those of the 14 weeks were seen. 2. PCNA positive cells were mainly mild expressed in the basal cell layer of normal oral mucosa, increased moderately, after 6 weeks. In suprabasal cell layer, control group is negative but retained moderately between 6 weeks and 14 weeks, and decreased after 16 weeks. In spinous cell layer, restricted only between 12 weeks and 16 weeks, other period is mild or negative. 3. PCNA index of experimental group revealed the increased peak in 6 weeks and 20 weeks than control group, and retained between 12 weeks and 18 weeks. All experimental group expressed higher PCNA index than control group(p.<0.05). 4. Tranglutaminase expression was localized in outer and suprabasal layers on control group, but after 6 weeks, expression site moved spinous & suprabasal cell layers, and after 8 weeks, expression is spreaded to basal cell layer, and this patters retained to 20 weeks. Transglutaminase expression of experimental group was higher than control group after 8 weeks. 5. The positive staining of detoxifying agent, G1utathione S-Transferase(GST)pi of experimental group was radually increased from 6 weeks. After 10 weeks, all layer of experimental group was seen positive reaction. The strong positive staining in center of tumor and weak positive staining in periphery of tumor were seen at the stage of squamous cell carcimoma in 14 weeks. According to the results, we should suggest that the more increased proliferation of tumor cell, the more increased expression of PCNA, transglutaminase and GST-pi as a detoxifying agent during carcinogenesis by induced DMBA were seen.
9,10-Dimethyl-1,2-benzanthracene* ; Animals ; Antibodies ; Carcinogenesis* ; Carcinoma in Situ ; Carcinoma, Squamous Cell ; Cell Death ; Cricetinae* ; Humans ; Infant ; Mesocricetus ; Mineral Oil ; Mouth Mucosa ; Mucous Membrane ; Paint ; Proliferating Cell Nuclear Antigen*

No abstract available.
Female ; Hysterectomy, Vaginal*

No abstract available.
Fractures, Open*

No abstract available.
Lower Extremity*

No abstract available.
Peripheral Nerves* ; Transcutaneous Electric Nerve Stimulation*

No abstract available.

BACKGROUND: Calcinosis cutis may occur when connective tissue is abnormal (dystrophic), or where calcium or phosphate levels in the blood are high(metastatic); alternatively, there may be no obvious underlying cause(idiopathic). The exact incidence of calcinosis cutis in dermatologic patients is not well-kniown and the pathomechanism of it remains to be elucidated. OBJECTIVE: This study was performed to analyze the incidence, clinical and histopathological features of calcinosis cutis. METHODS: We studied 72 patients with calcinosis cutis who visited our department between January 1985 and December 1996. The patients were classified into 3 types (dystrophic, metastatic, and idiopathic) and were analyzed clinically and histopathologically. Results : The results were summerized as follows; 1. There were 60 cases(83.3%) and 12 cases(16.7%) of the dystrophic and idiopathic types, respectively but the metastatic type was not observed. The frequency of dystrophic calcification in individual disorders was 88.2% in pseudoxanthoma elasticum, 66.7% in trichilemmal cyst and dermatomyositis, and 64.6% in pilomatrichoma. 2. In the idiopathic type, it was common in females aged over 50 years and the mean duration of lesions was 2.8+/-2.0 years. The predilection site was the flank(5 cases) and most of the lesions(7 cases) showed skin-colored nodules 3. Histopathologically, calcified material of the idiopathic type was located predominently in the dermis(10 cases) and was present as large masses surrounded by a foreign body reaction. 4. In the dystrophic type, calcified deposits were noted predominently within the shadow cells of pilomatricoma, in the degenerated elastic fibers of pseudoxanthoma elasticum, and in the keratinized area of basal cell carcinoma, squamous cell carcinoma, trichilemmal cyst, and epidermal cyst. Foreign body reactions with giant cells and mononuclear cell infiltrations were often found around large deposits of calcium. Conclusion : The incidence of calcinosis cutis in dermatologic patients is relatively low, but a variety of disorders can be associated with cutaneous calcification. Therefore, dermatologists should be familiar with the different forms of cutaneous calcification and the dermatoses that manifest them.
Calcinosis* ; Calcium ; Carcinoma, Basal Cell ; Carcinoma, Squamous Cell ; Connective Tissue ; Dermatomyositis ; Elastic Tissue ; Epidermal Cyst ; Female ; Foreign Bodies ; Foreign-Body Reaction ; Giant Cells ; Humans ; Incidence ; Pilomatrixoma ; Pseudoxanthoma Elasticum ; Skin Diseases

No abstract available.
Chronic Pain* ; Electroacupuncture* ; Needles* ; Syringes*

Serial neurosonographic examinations are routinely performed at frequent intervals during nursery course of all preterm infants of very low-birth-weight who are admitted to the intensive care nursery of Presbyterian Medical Center from November 1, 1990 to July 30, 1992. After discharge, the following survivors who had received periodic, serial scanning by meas of cranial ultrasonography were longitudinally observed in an interdisciplinary neurodevelopmental follow-up program to a mean corrected age of 13 months. Neurodevelopmental outcome was assessed by means of Vojta's postural reaction and other neurological examinations. The results are as follows: 1) The incidence of PV-IVH in the study was 79%. 2) According to Papile's grading system of PV-IVH, gradel was 20%, gradell was 46%, gradelll was 19%, and grade IV was 13%. 3) The risk factors associated with PV-IVH were birth weight, gestational age, apgar score, ventilator care, RDS, and sepsis. 4) The mortality of PV-IVH was 20% for gradel, 19% for gradell, 44% for gradelll, and 67% for grade lV. 5) According to relationship between PV-IVH and neurodevelopmental outcome, in two of the four subjects with grade lll PV-IVH, moderate/severe CCD was developed. 6) According to relationship between PVE with cysts and nuerodevelopmental outcome, moderate/severe PVE with periventricular cysts larger than 3mm in diameter was associated with development of severe CCD.
Apgar Score ; Birth Weight ; Follow-Up Studies ; Gestational Age ; Hemorrhage* ; Humans ; Incidence ; Infant, Newborn ; Infant, Premature* ; Critical Care ; Mortality ; Neurologic Examination ; Nurseries ; Protestantism ; Risk Factors ; Sepsis ; Survivors ; Ultrasonography ; Ventilators, Mechanical

No abstract available.
Adrenocorticotropic Hormone* ; Hydrocortisone* ; Transcutaneous Electric Nerve Stimulation*