A case of neonatal diabetes mellitus is described. The child presented with low birth weight but was normal in appearance. She was acidotic and ketonuria was observed. The HLA typing was DR1 and 3, and insulin autoantibodies were negative. Genetic analysis with polymorphic DNA markers for chromosome 6 indicated biparental inheritance. She required insulin therapy for the control of hyperglycemia, and insulin dependence continues after 8 months of age.
Autoantibodies ; Child ; Chromosomes, Human, Pair 6 ; Diabetes Mellitus* ; Genetic Markers ; Histocompatibility Testing ; Humans ; Hyperglycemia ; Infant, Low Birth Weight ; Infant, Newborn ; Insulin ; Ketosis ; Wills

No abstract available.
Coffee* ; Denture Bases* ; Dentures*

The real time high resolution mechanical neurosonographic sector scanner is a convenient and useful instrumentfor detection of intracranial lesions in premature infants as well as low birth weight. From this experience,authors report with 104 neonates and infants who had been suspected intracranial hemorrhage and had been detectedand graded by sonography. The results were as followed: 1. The incidence of intracranial hemorrhage is 20 of 46(43.5%) neonates who had been studied before 3 days of age. But 7 of 16(43.7%) neonates showed ICH between the 4-7days of age. 2. There was no difference of prevalence in sex ratio in neonates with ICH. 3. There was closerelationship between ICH and gestational weeks. 15 of 27 (55.6%) neonates who were born under 32 gestational weeksshowed ICH was graded as 3 or 4 in 7 of 10. 4. 10 of 16(62.5%) neonate who were born under 1,500 gram body weighthad ICH within first 4 days. The severity of ICH was graded as 3 or 4 in 7 of 10. 5. The mode of deliveryinfluenced neonatal ICH incase of difficulty delivery such as forcep(4/4, 100%), vacuum (7/10, 70%), breech (3/3,100%), and C/S(13/33, 39.4%). 6. Incidence of ICH in neonates with pathologica lung conditions (29/40, 72.5%) washigher than ICH with normal chest findins(15/64, 23.4%). 7. For frequent follow up study of brain, neurosonographywas very helpul and economic in case of ICH, and could find congenital glioblastoma multiformed(1), and low gradeastrocytoma(1) incidentally.
Brain ; Follow-Up Studies ; Glioblastoma ; Humans ; Incidence ; Infant ; Infant, Low Birth Weight ; Infant, Newborn ; Infant, Premature ; Intracranial Hemorrhages ; Lung ; Prevalence ; Sex Ratio ; Thorax ; Vacuum

A clinical observation was made of 14 cases of Wilms' tumor admitted to the Department of Urology, Keimyung University Medical College and Hospital during the period from January 1973 through May 1981. Clinical findings and therapeutic modalities with prognosis are summarized below: 1. The range in age is from 2 months to 11 years, average 3.9 years. 2. Common symptoms are painless palpable abdominal mass, abdominal pain and hematuria. 3. The characteristic urographic findings are as follows: Calyceal distortions in 7 cases, non-visualizing kidney in 3 cases and hydronephrosis in 2 cases. Lung metastases were noted in 5 cases on chest X-ray. 4. Clinical stages according to the National Wilms` Tumor Study criteria are as follows : Stage I in 6 cases. II in 1 case, III in 2 cases and IV in 5 cases. 5. Modalities of treatment are chiefly as follows: Nephrectomy only in 2 cases, Nephrectomy+radiation therapy in 2 cases, Nephrectomy+radiation therapy+single AMD course in 2 cages and Nephrectomy+multiple AMD & VCR course in 6 patients. 6. 2 and 1/2 year survival rates according to the clinical stages are as follows: Stage I 4/4 II 1/1 III 1/2 and IV 1/2. 7. 2 and 1/2 year survival rates according to the age groups are as follows : -2 years 4/4, 2 years - 5 years 0/2, and 5 years -2/3. 8. Side effects of multiple AMD & VCR courses are as follows: Vomiting 4, alopecia 4, leukopenia 2, anemia 1, abdominal pain 1, fever 1, Those were corrected with conservative treatment.
Abdominal Pain ; Alopecia ; Anemia ; Drug Therapy* ; Fever ; Hematuria ; Humans ; Hydronephrosis ; Kidney ; Leukopenia ; Lung ; Neoplasm Metastasis ; Nephrectomy ; Prognosis ; Survival Rate ; Thorax ; Urology ; Vomiting ; Wilms Tumor*

Each three rabbit corneas were stored in McCarey-Kaufman medium at 4 degrees C for 5, 9 and 14 days and used as donor materials in 6mm partial penetrating corneal allografts. These results were compared to that group of penetrating keratoplasties with corneas stored conventional moist chamber at 4 degrees C for 5 days. All the M-K media stored corneas for 5 and 9 days were in clear graft after keratoplasty during the observation period of 6 months but the corneas stored for 14 days were developed eventvally irreversilde opaque after grafts. At 6 months after keratoplasties allthe rabbits eyes were enucleated and sectioned for histopathologic study of endothelia with the light and electron microscopes. Endothelia of the corneas stored for 5 and 9 days showed rarely endothelial degenerative changes. But the corneas stored up to 14 days showed significant changes including of disappearanee of most endothelial membranes and cytoplasms in light microscopies and swelling of nucleus, dispersed chromatin clump, multiple small and large vacuoles in cytoplasm and occasional autolysis of cytoplasm were observed in electron microscopies.
Allografts* ; Autolysis ; Chromatin ; Cornea* ; Corneal Transplantation ; Cytoplasm ; Humans ; Keratoplasty, Penetrating ; Membranes ; Microscopy ; Rabbits ; Tissue Donors ; Transplants ; Vacuoles

No abstract available.
Genetic Therapy*

Malignant fibrous histiocytoma (MFH) of the heart is a very rare disease. It is difficult to distinguish from myxoma which is most common benign cardiac tumor. Prognosis of malignant fibrous histiocytoma (MFH) of the heart is known to be fatal despite of medical and surgical treatment in contrast to benign tumors. A primary myxoid malignant fibrous histiocytoma of the left atrium was diagnosed in a 58 years old woman. The mass was removed incompletely by operation. The patient did not receive either chemotherapy or radiotherapy. However, the patient is still alive for more than fifteen months after the initial presentation. We report a case of malignant fi brous histiocytoma (MFH) with reviewing the current references.
Drug Therapy ; Echocardiography ; Female ; Heart Atria ; Heart Neoplasms ; Heart* ; Histiocytoma ; Histiocytoma, Malignant Fibrous* ; Humans ; Middle Aged ; Myxoma ; Prognosis ; Radiotherapy ; Rare Diseases

BACKGROUNDS: The molecular genetic characteristcs of cis-AB blood group have shown that its allele had C, G, C and C at nucleotide positions (nps) 526, 703, 796 and 803, respectively. And all cis-AB analysed and reported molecular genetically in Korea and Japan had T at np 467 (leucine at amino acid position 156). We report a first case of cis-AB with C at np 467 (proline at amino acid position 156). METHODS: Genomic DNA was extracted from peripheral blood of cis-AB patient and amplified by DS1/DS2 and DS3/DS4 allele-specific primers. After PCR, we analysed nps 261, 467, 526, 646, 703, 796, and 803 by restriction digestion, autoradiography and automatic sequencing. RESULTS: PCR-RFLP with DS1/DS2 primers and restriction enzyme KpnI showed that cis-AB had an 0 allele. The results of genomic sequencing, autoradiography and restriction digestion showed that cis-AB allele at nps 467, 526, 646, 703, 796 and 803 had C, C, T, G, C and C, respectively. CONCLUSION: This cis-AB showed characteristic molecular genetic features at nps 526, 703, 796, and 803. And this is a first case of A(Pro) cis-AB with C at np 467. (Korean J Blood Transfusion 10(1): 13-19, 1999)
Alleles ; Autoradiography ; Blood Transfusion ; Cytosine* ; Digestion ; DNA ; Humans ; Japan ; Korea ; Molecular Biology* ; Polymerase Chain Reaction

We examined the amyloid deposit in various malignant cutaneous epithelial tumors by using Dylon stain. Histochemically identifiable amyloid deposits associated with basal cell epitheliomas(BCEs), squamous cell carcinomas(SCCs) and Bowens diseases were studied with monoclonal cytokeratin antibodies and anti-amyloid P antibodies. The results were as follaws, l. Amyloid deposits were detected in 5 of 12 BCEs, 4 of 11 SCCs, 3 of 9 Bowens diseases and 1 of 8 actinic keratoses. Amyloid deposits were not detected in o keratoacanthomas, 2 verrucous carcinomas, 1. Pagets disease and 3 extramammary Pagets diseases. 2. Anti-keratin antibody 34BE12 and anti-amyloid P antibody reacted with amyloid deposits in 3 of 5 BCEs which showed abundant amyloid deposits by Dylon stain. 3. Of 4 SCCs which showed amyloid deposits by Dylon stain, anti-keratin ant.ibody 34pE12 reacted with amyloid deposits in 2 cases and anti-amyloid P antibody reacted with amyloid deposits in 3 cases. 4. Anti-amyloid P antibody reacted with arnyloid deposits in 3 of 3 Bowens diseases which showed arnyloid deposits by Dylon stain. Anti-keratin antibody 34pE12 reacted with amyloid deposits only 1 of them. These findings suggest that epidermal keratins are the percursor substance of amyloid in malignant cutaneous epithelial tumors. The difference of antikeratin staining pattern between BCE-associated amyloid and SCC or Bowens disease-associated amyloid may be the results of difference in development stage of cutaneous amyloids.
Amyloid* ; Antibodies ; Carcinoma, Verrucous ; Keratins ; Keratoacanthoma ; Keratosis, Actinic ; Plaque, Amyloid*

We examined the p53 protein overexpression and evaluated its correlation with pathobiological variables, including: (1) patient age, sex, tumor size, histological type and grade, invasion depth, vascular invasion, perineural invasion and lymph node status; (2) the Ki-67 labeling index in 100 gastric carcinomas; and (3) the DNA ploidy pattern, S phase fraction (SPF), and the proliferation index (PI) in 84 cases using flow cytometry. The positive rate of p53 staining was 48% and the p53 immunoreactivity was independent of variable clinicopathologic factors. No correlation was made between the Ki-67 labeling index with p53 immunostaining and DNA ploidy parameters. Aneuploidy rate was slightly higher in the p53 positive group (55.6%) than the p53 negative group (44.4%)(p=0.097). The mean values of SPF and PI were significantly higher in the p53 protein positive group. Aneuploidy was more often observed in the intestinal type (p=0.038), advanced gastric carcinoma (p=0.015) and lymph node positive group(p=0.039). The above results suggest that although the p53 protein overexpression has no significant correlation with pathological factors and the Ki-67 labeling index, it may play an important role in tumor cell proliferation. Since p53 protein overexpression was slightly higher in the aneuploidy group showing significant correlation with poor prognostic parameters, it is thought that re-evaluation of the p53 mutation by molecular biological study is needed.
Aneuploidy ; Cell Proliferation ; DNA* ; Flow Cytometry ; Humans ; Lymph Nodes ; Ploidies* ; S Phase