1.Hepatoid carcinoma of the pancreas: a case report.
Chong-zhong LIU ; San-yuan HU ; Lei WANG ; Xu-ting ZHI ; Bin JIN ; Min ZHU ; Mitchell S WACHTEL ; Eldo E FREZZA
Chinese Medical Journal 2007;120(20):1850-1852
Aged
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Aged, 80 and over
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Carcinoma, Hepatocellular
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diagnosis
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pathology
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surgery
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Humans
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Keratin-18
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analysis
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Keratin-19
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analysis
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Male
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Pancreatic Neoplasms
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diagnosis
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pathology
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surgery
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alpha-Fetoproteins
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analysis
2.Noninvasive predictors of nonalcoholic steatohepatitis in Korean patients with histologically proven nonalcoholic fatty liver disease.
Young Seok KIM ; Eun Sun JUNG ; Wonhee HUR ; Si Hyun BAE ; Jong Young CHOI ; Myeong Jun SONG ; Chang Wook KIM ; Se Hyun JO ; Chang Don LEE ; Young Sok LEE ; Sang Wook CHOI ; Jin Mo YANG ; Jeong Won JANG ; Sang Gyune KIM ; Seung Won JUNG ; Hee Kyung KIM ; Hee Bok CHAE ; Seung Kew YOON
Clinical and Molecular Hepatology 2013;19(2):120-130
BACKGROUND/AIMS: The aims of this study were (1) to identify the useful clinical parameters of noninvasive approach for distinguishing nonalcoholic steatohepatitis (NASH) from nonalcoholic fatty liver disease (NAFLD), and (2) to determine whether the levels of the identified parameters are correlated with the severity of liver injury in patients with NASH. METHODS: One hundred and eight consecutive patients with biopsy-proven NAFLD (age, 39.8+/-13.5 years, mean+/-SD; males, 67.6%) were prospectively enrolled from 10 participating centers across Korea. RESULTS: According to the original criteria for NAFLD subtypes, 67 patients (62.0%) had NASH (defined as steatosis with hepatocellular ballooning and/or Mallory-Denk bodies or fibrosis > or =2). Among those with NAFLD subtype 3 or 4, none had an NAFLD histologic activity score (NAS) below 3 points, 40.3% had a score of 3 or 4 points, and 59.7% had a score >4 points. Fragmented cytokeratin-18 (CK-18) levels were positively correlated with NAS (r=0.401), as well as NAS components such as lobular inflammation (r=0.387) and ballooning (r=0.231). Fragmented CK-18 was also correlated with aspartate aminotransferase (r=0.609), alanine aminotransferase (r=0.588), serum ferritin (r=0.432), and the fibrosis stage (r=0.314). A fragmented CK-18 cutoff level of 235.5 U/L yielded sensitivity, specificity, and positive and negative predictive values of 69.0%, 64.9%, 75.5% (95% CI 62.4-85.1), and 57.1% (95% CI 42.2-70.9), respectively, for the diagnosis of NASH. CONCLUSIONS: Serum fragmented CK-18 levels can be used to distinguish between NASH and NAFL. Further evaluation is required to determine whether the combined measurement of serum CK-18 and ferritin levels improves the diagnostic performance of this distinction.
Adult
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Aged
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Aged, 80 and over
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Alanine Transaminase/blood
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Asian Continental Ancestry Group
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Aspartate Aminotransferases/blood
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Biological Markers/blood
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Fatty Liver/classification/metabolism/*pathology
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Female
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Ferritins/blood
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Fibrosis/complications
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Humans
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Keratin-18/analysis
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Male
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Middle Aged
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Predictive Value of Tests
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Prospective Studies
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Republic of Korea
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Severity of Illness Index
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Young Adult
3.Induced differentiation of rat hepatic oval cells in-vitro by combined hepatocyte growth factor and epidermal growth factor treatment.
Jun LIU ; Ling XUE ; Meng ZHANG ; Li-hong CHE ; Hui-xi WU ; Rui-de HU
Chinese Journal of Pathology 2007;36(11):756-759
OBJECTIVETo characterize the biologic featrues of hepatic oval cells and their protein expression profiles during induced differentiation in vitro.
METHODSRat hepatic oval cells were treated with epidermal growth factor (EGF) and hepatocyte growth factor (HGF) in vitro, followed by morphological and molecular marker assessment by electromicroscopy, immunocytochemistry, RT-PCR and protein expression chip technology.
RESULTSTen weeks after induction, the levels of GST-P mRNA and M2-PK mRNA were significantly reduced, whereas those of ALB and CK18 were elevated. Significant variations of expression was seen in 8 protein species during the course of the induced differentiation.
CONCLUSIONCombined EGF and HGF treatment in vitro induces cell differentiation of hepatic oval cells, a process in which 8 protein species may play some regulatory roles.
Albumins ; metabolism ; Animals ; Cell Differentiation ; drug effects ; Epidermal Growth Factor ; pharmacology ; Glutathione Transferase ; biosynthesis ; genetics ; Hepatocyte Growth Factor ; pharmacology ; Hepatocytes ; cytology ; metabolism ; ultrastructure ; Immunohistochemistry ; Keratin-18 ; metabolism ; Protein Array Analysis ; Pyruvate Kinase ; biosynthesis ; genetics ; RNA, Messenger ; metabolism ; Rats ; Reverse Transcriptase Polymerase Chain Reaction