No abstract available.
Animals ; Mice*

No abstract available.

Forty children were anesthetized with either thiopental 5mg/kg followed by succinylcholine 1.5mg/kg I.V. or with isoflurane 3.5 vol% in 66% nitrous oxide in oxygen via a face mask followed by succinylcholine 1.5mg/kg I.V.. The study was randomized, in the thiopental group, fasciculation were seen in all children and in the isoflurane group, in 10 of 20 children(p<0.001). The intensity of the fasciculation was 1.6+/-0.7 in the thiopental group and 0.6+/-0.4 in the isoflurane group(p<0.001). The duration of fasciculstion was 35.6+/-13.6sec in the thiopental group and 9.0+/-8.0 sec in the isoflurane group(p<0.001). No cardiac arrhythmias were noted in either group, but heart rates were inereased significantly after intubation in both group(p<0.001). ln conclusion, isoflurane in nitrous oxide may inhibit succinylcholine-induced muscle fasciculation in children.
Anesthesia* ; Arrhythmias, Cardiac ; Child* ; Fasciculation* ; Heart Rate ; Humans ; Intubation ; Isoflurane* ; Masks ; Nitrous Oxide ; Oxygen ; Succinylcholine* ; Thiopental

The efficacy of air-polishing on subgingival debridement, as compared to scaling and root planning (SRP), was evaluated clinically and microbiologically. Fifteen patients diagnosed as chronic periodontitis, and having single-root tooth over 5 mm of pocket depth symmetrically in the left and right quadrant, were investigated. Subgingival debridement was performed by SRP and air-polishing. The results were evaluated and compared clinically and microbiologically. Probing pocket depth (PPD), bleeding on probing (BOP), relative attachment level (RAL) and change of gingival crevicular fluid (GCF) were assessed before treatment, and at 14 and 60 days after treatment. Microbial analysis was done pre-treatment, post-treatment, and at 14 and 60 days after treatment. Results of air polishing showed that post treatment, the PPD and BOP decreased, and attachment gain was observed. There was no clinical difference when compared to SRP. The volume of GCF decreased at 14 days, and increased again at 60 days. Compared to SRP, there was a statistical significance of the volume of GCF at 60 days in air-polishing. In the microbial analysis, high-risk bacteria that cause periodontal disease were remarkably reduced. They decreased immediately after treatment, but increased again with the passage of time. Thus, our results show that subgingival debridement by air-polishing was effective for decrease of pocket depth, attachment gain, decrease of GCF and inhibition of pathogens. Further studies are required to compare air-polishing and SRP, considering factors such as degree of pocket depth and calculus existence.
Bacteria ; Calculi ; Chronic Periodontitis ; Debridement ; Gingival Crevicular Fluid ; Hemorrhage ; Humans ; Periodontal Debridement ; Periodontal Diseases ; Root Planing ; Tooth

PURPOSE: The purpose of this study was to evaluate the effects of platelet-rich plasma (PRP) on periodontal healing of replanted root surfaces in dogs histologically and histomorphometrically. METHODS: A total of 36 roots of mandibular incisors and premolars from 6 mongrel dogs were used. The roots were randomly divided into 3 groups: 1) a positive control group (n=12), in which the periodontal ligament (PDL) and cementum were retained and the roots were soaked in saline; 2) a negative control group (n=12), in which the PDL and cementum were removed and the roots were soaked in saline; and 3) an experimental group (n=12), in which the PDL and cementum were removed and the roots were soaked in PRP. After soaking the root surfaces, the extracted roots were replanted into the extraction sockets. The roots were covered using a coronally repositioned flap RESULTS: Histologically, irregular-thickness PDL-like and cementum-like tissues were observed in the 4-week experimental group and the positive control group. PDL-like tissue and cementum-like tissue with a more uniform thickness were observed at 8 weeks. In the negative control group, PDL-like tissue and cementum-like tissue were rarely found, and root resorption and ankylosis were observed. In the cross-sectional histomorphometric analysis, the experimental group demonstrated a higher rate of formation of cementum-like tissue and a lower tooth ankylosis rate than the positive and negative control groups at 4 and 8 weeks. Although there was a significant difference in the tooth ankylosis rate and the formation of cementum-like tissue across the 3 groups (P < 0.05), no statistical significance was observed between any pair of groups (P > 0.017). CONCLUSIONS: Applying PRP to root surfaces during tooth replantation in dogs can reduce tooth ankylosis and increase PDL-like and cementum-like tissue formation.
Animals ; Ankylosis ; Bicuspid ; Clothing ; Dental Cementum ; Dogs ; Incisor ; Periodontal Ligament ; Periodontitis ; Platelet-Rich Plasma ; Root Resorption ; Tooth Ankylosis ; Tooth Replantation ; Tooth

OBJECTIVE: The objectives of this study were to evaluate the immediate and long-term efficacy and safety of coil embolization for large or giant aneurysms.METHODS: One hundred and fifty large or giant aneurysm cases treated with endovascular coil embolization between January 2005 and February 2014 at a single institute were included in this study. Medical records and imaging findings were reviewed. Statistical analysis was performed to evaluate prognostic factors associated with major recurrence (major recanalization or rupture) and delayed thromboembolism after selective coil embolization.RESULTS: Procedure-related symptomatic complications occurred in five (3.3%) patients. The mean clinical and radiological follow-up periods were 38 months (range, 2–110) and 26 months (range, 6–108), respectively. During the follow-up period, the estimated recurrence rate was 4.6% per year. Multivariate analysis using Cox regression showed the degree of occlusion to be the only factor associated with recurrence (p=0.008, hazard ratio 3.15, 95% confidence interval 1.34–7.41). The patient’s history of rupture in addition to the size and location of the aneurysm were not associated with recurrence in this study. Delayed infarction occurred in eight cases, and all were incompletely occluded.CONCLUSION: Although immediate postprocedural safety profiles were reasonable, longterm results showed recanalization and thromboembolic events to occur continuously, especially in patients with incomplete occlusion. In addition, incomplete occlusion was associated with delayed thromboembolic complications. Patients with incomplete occlusions should be followed carefully for delayed recurrence or delayed thromboembolic events.
Aneurysm ; Embolization, Therapeutic ; Endovascular Procedures ; Follow-Up Studies ; Humans ; Infarction ; Intracranial Aneurysm ; Medical Records ; Multivariate Analysis ; Recurrence ; Rupture ; Thromboembolism ; Treatment Failure

Paragonimiasis is a lung fluke of human and usually found in the lungs but may be found in many unusual locations such as pleura, abdominal wall and brain etc. A 16-year old female patient visited department of Gynecology due to abdominal pain and distension. She took an examination with ultrasonography for ascites or pelvic cystic mass evaluation, diagnostic laparoscopy with omental biopsy for tissue diagnosis, and parasite specific antibody test (ELISA) for paragonimiasis. We made the diagnosis of Omental Paragonimiasis based on P. westermani eggs in eosinophils containing granuloma by laparoscopic mesenteric lymph node biopsy and specific IgE antibody to P. westermani. She was treated with praziquantel for three days and discharged. Here, we report a case of omental paragonimiasis with literature review, because this rarely involves the omentum and may be confused with other abdominopelvic inflammatory diseases, particularly intestinal tuberculosis in Korea.
Abdominal Pain ; Abdominal Wall ; Adolescent ; Ascites ; Biopsy ; Brain ; Diagnosis ; Eggs ; Eosinophils ; Female ; Granuloma ; Gynecology ; Humans ; Immunoglobulin E ; Korea ; Laparoscopy ; Lung ; Lymph Nodes ; Omentum ; Ovum ; Paragonimiasis* ; Parasites ; Pleura ; Praziquantel ; Trematoda ; Tuberculosis ; Ultrasonography

Transplantation of marrow-derived mesenchymal stem cells (MSCs), expanded by culture in addition to whole bone marrow, has been shown to enhance engraftment of human hematopoietic stem cells (HSCs). Our hypothesis was that there might be an optimum ratio range that could enhance engraftment. We examined the percent donor chimerism according to the ratio of HSCs to MSCs in non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. We tested a series of ratios of co-transplanted CD34+-selected bone marrow cells, and marrow-derived MSCs into sublethally irradiated NOD/SCID mice. In all experiments, 1 x 10(5) bone marrow derived human CD34+ cells were administered to each mouse and human MSCs from different donors were infused concomitantly. We repeated the procedure three times and evaluated engraftment with flow cytometry four weeks after each transplantation. Serial ratios of HSCs to MSCs were 1:0, 1:1, 1:2 and 1:4, in the first experiment, 1:0, 1:1, 1:2, 1:4 and 1:8 in the second and 1:0, 1:1, 1:4, 1:8 and 1:16 in the third. Cotransplantation of HSCs and MSCs enhanced engraftment as the dose of MSCs increased. Our results suggest that the optimal ratio of HSCs and MSCs for cotransplantation might be in the range of 1:8-1:16; whereas, an excessive dose of MSCs might decrease engraftment efficiency.
Middle Aged ; Mice, SCID ; Mice, Inbred NOD ; Mice ; Mesenchymal Stem Cells/*cytology ; Mesenchymal Stem Cell Transplantation/*methods ; Humans ; Hematopoietic Stem Cells/*cytology ; Hematopoietic Stem Cell Transplantation/*methods ; Graft Survival/*physiology ; Cells, Cultured ; Cell Count ; Animals ; Adult

Transplantation of marrow-derived mesenchymal stem cells (MSCs), expanded by culture in addition to whole bone marrow, has been shown to enhance engraftment of human hematopoietic stem cells (HSCs). Our hypothesis was that there might be an optimum ratio range that could enhance engraftment. We examined the percent donor chimerism according to the ratio of HSCs to MSCs in non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice. We tested a series of ratios of co-transplanted CD34+-selected bone marrow cells, and marrow-derived MSCs into sublethally irradiated NOD/SCID mice. In all experiments, 1 x 10(5) bone marrow derived human CD34+ cells were administered to each mouse and human MSCs from different donors were infused concomitantly. We repeated the procedure three times and evaluated engraftment with flow cytometry four weeks after each transplantation. Serial ratios of HSCs to MSCs were 1:0, 1:1, 1:2 and 1:4, in the first experiment, 1:0, 1:1, 1:2, 1:4 and 1:8 in the second and 1:0, 1:1, 1:4, 1:8 and 1:16 in the third. Cotransplantation of HSCs and MSCs enhanced engraftment as the dose of MSCs increased. Our results suggest that the optimal ratio of HSCs and MSCs for cotransplantation might be in the range of 1:8-1:16; whereas, an excessive dose of MSCs might decrease engraftment efficiency.
Middle Aged ; Mice, SCID ; Mice, Inbred NOD ; Mice ; Mesenchymal Stem Cells/*cytology ; Mesenchymal Stem Cell Transplantation/*methods ; Humans ; Hematopoietic Stem Cells/*cytology ; Hematopoietic Stem Cell Transplantation/*methods ; Graft Survival/*physiology ; Cells, Cultured ; Cell Count ; Animals ; Adult

PURPOSE: To determine the risk factors relating renal allograft and patient survival, we, Dae Jeon St. Mary's Hospital transplantaion team, Dae Jeon, Korea, reviewed 200 cases of kidney transplantation. METHODS: 200 medical records of kidney transplantation from February 1988 to June 2000 was reviewed retrospectively. The clinical follow up period was February 2001, and clinical analysis was done. RESULTS: 1) The original renal disease of the cases were 78 cases of chronic glomerulonephritis, 18 cases of diabetic nephropathy, 16 cases of hypertensive nephrosclerosis and 1 case of lupus nephritis, 2) The recipient-donor relationships were 14 cases of parent to offspring, 9 cases of offspring to parents, 26 cases of between siblings. There were 150 cases of non- related donor and 1 case of cadaveric donor, 3) At the end of Feb. 2001, 33 graft and 10 patients were lost (5 year graft survival was 88.3% and 5 year patient survival rate was 94.6%), 4) 7 cases of malignant tumors, 171 cases of hypertension, 141 cases of hyperlipdemia, 76 cases of Cushing's disease and 58 cases of hyperuricemia were developed, 5) There were 114 cases of infections (41 cases of bacterial infections, 40 cases of viral infections, 13 cases of tuberculosis and 20 cases of fungal infections), 6) The cases of surgical complications were 16 cases of lymphocele, 9 cases of urinary leakage, 5 cases of hematome and other 3 cases, 7) The factor analysis for graft survival showed that the donor and recipient age, number of acute rejection episodes had statistical significance. CONCLUSION: Episodes of acute rejection and old age group both in donor and recipient over 50 were the risk factors affecting renal allograft survival.
Allografts ; Bacterial Infections ; Cadaver ; Daejeon ; Diabetic Nephropathies ; Follow-Up Studies ; Glomerulonephritis ; Graft Survival ; Humans ; Hypertension ; Hyperuricemia ; Kidney Transplantation* ; Kidney* ; Korea ; Lupus Nephritis ; Lymphocele ; Medical Records ; Nephrosclerosis ; Parents ; Retrospective Studies ; Risk Factors ; Siblings ; Survival Rate ; Tissue Donors ; Transplants ; Tuberculosis