Objective To investigate the prevalence of target organ damage in a cohort of hospitalized patients with hypertension.Methods This retrospective analysis evaluated the prevalence of cardiovascular complications in hypertensive patients hospitalized in the Department of Hypertension at the Province Hospital of Xinjiang.Results ① Among 3783 hospitalized patients with hypertension,the prevalence of of cerebrovascular disease,coronary heart disease,chronic renal failure was 23.0%,17.3% and 3.8%,respectively.The prevalence of patients who had ≥1 complications was 39.1%.② Cerebrovascular disease comprised 3.2% of hemorrhagic stroke,87.9% of ischemic stroke,and 8.8% of mixed stoke.Coronary heart disease included with 1.7% of asymptomatic myocardial ischemia,84.0% of angina(unstable 46.5% and stable 53.5%),9.3% of myocardial infarction,8.1% of ischemic cardiomyopathy,whereas in chronic renal failure category,40.0% was of compensatory stage,45.5% of azotemic stage,and 14.5% of uremic stage.Conclusion Our data showed 40% hospitalized patients with hypertension had target organ damages.Stroke,coronary heart disease and renal dysfunction are three major complications.

Objective To study the estrogenic effect of organic extracts of effluent samples from different treatment stages in Tianjin reclaimed water treatment plant. Methods Reverse phase C-18 solid-phase extraction (RP-C18SPE) was used for the extraction of target compounds from water samples. Then, a simplified proliferation test with human estrogen receptor-positive MCF-7 breast cancer cell and recombined yeast assay were applied to assess the estrogenic effect in effluent samples from different treatment stages in Tianjin reclaimed water treatment plant. Results The amount of extracts of reclaimed water inducing the maximum effect in cell proliferation assay was equal to that from 1.00 ml primary water. In recombined yeast assay, the amount of extracts of reclaimed water inducing maximum ?-galactosidase activity was equal to that from 20.00 ml primary water. Conclusion The effluent of reclaimed water treatment plant still shows estrogenic effect though slightly reduced.

Objective To evaluate the causes and prognosis of severe trauma in the elderly.Methods The 168 patients in elderly group (aged 60 to 91 years), 517 in middle-aged group (aged 36to 59 years) and 405 in young group (aged 18 to 35 years) were evaluated using an abbreviated injury scale (AIS2005) and injury severity score (ISS). All patients with ISS ≥ 16 were selected during a seven-year period. The injury severity, injury site number, cause of injury, injury site, emergency operation, diseases before injury, secondary infection after injury, development of multiple organ dysfunction, number of patients with Intensive Care Unit (ICU) stay, length of stay in ICU and prognosis were compared among three groups. Results The main cause of injury was accident (64patients, 38.1%), followed by traffic accident (63 patients, 37.5%) in elderly group. The traffic accident was major cause of injury in middle-aged and young group (246 patients, 47.6%; 153patients, 37.8%, respectively), followed by fall from high places (128 patients, 24.8%; 102 patients, 25.2%, respectively). The main injury sites were head and chest in elderly, middle-aged and young group (155 patients, 92.3%; 411 patients, 79.5%; 321 patients, 79.3%, respectively).There were significant differences among three groups in injury site number, emergency operation,pre-injury diseases, secondary infection after injury, number of patients with ICU stay and length of stay in ICU (F=8. 299, P＜0.01; x2= 14.88, P=0.001; x2=254.6, P＜0.01; x2=10. 54, P=0. 005; x2 = 15.62, P＜0.01; F= 5.760, P= 0.005, respectively ). In spite of injury severity (F=2.950, P= 0.053), there were significant differences between elderly group and middle-aged or young group (t=2.325, P=0.021; t=2.128, P=0.034, respectively). The incidence of multiple organ dysfunction had no significant difference among the three groups (x2 = 1.142, P= 0.565). The cure rate and unhealed automatically discharged patients had significant differences (x2 = 13.77, P= 0. 001;x2 =6.025, P= 0.049, respectively). The mortalities were similar (x2 = 1.397, P= 0.497). The leading cause of death among three groups was a serious head injury. Conclusions For elderly patients, it is important to reduce accidental injuries and traffic accidents, to improve the cure rate,and to reduce the unhealed and mortality rate.

Purpose To explore the value of hydroxyapatite (HAP) in the measurement of bone mineral density (BMD) based on the quantitative material decomposition images of spectral CT on healthy adult females.Materials and Methods A total of 128 healthy females who underwent upper abdominal CT examination with spectral CT at the Second Hospital of Lanzhou University from September 2013 to February 2016 were enrolled as the research group.Those patients with trauma,surgery,tumor or other diseases affecting BMD were excluded.The patients (ages ranged from 18 to 87 years) were divided into 6 groups according to their ages:＜30 years (n=23),30-39 years (n=20),40-49 years (n=22),50-59 years (n=24),60-69 years (n=19) and ≥ 70 years (n=20).The HAP and calcium concentration at central part of L2 centrum of spongy bone were measured.Meanwhile,119 healthy females who underwent dual energy X-ray absorption (DEXA) examination were selected as the controls and also divided into groups according to the same criteria as the research group.The BMD of the controls was also measured at L2 centrum and showed as areal density (g/cm2).The measurements of the research group were analyzed.The correlation analysis was done between hydroxyapatite,calcium concentration and age.The correlations between hydroxyapatite,calcium concentration and BMD obtained by DEXA were also analyzed.Results There were significant differences in the HAP and calcium concentration among different age groups (P＜0.05).The results of spectral CT and the DEXA showed correlations.Both HAP and calcium concentration showed positive relationship with BMD obtained by DEXA (r=0.874 and 0.796,respectively,both P＜0.05).The HAP and calcium concentration showed positive relationship with age in the groups (ages ranged from 18-39 years) (r=0.538 and 0.416,P＜0.05) and negative relationship with age in the groups (ages over 40 years) (r=-0.629 and-0.562,P＜0.05).Conclusion Material decomposition images of spectral CT can reflect bone changes,and HAP is a new base material for BMD measurement.

This study is to investigate the effect of 8-prenylnaringenin (8-PNG) on osteoclastogensis of bone marrow cells and bone resorption activity of osteoclasts. Osteoclasts were separated from long bone marrow of newborn rabbits and cultured in alpha-MEM containing 10% FBS. 8-PNG was added into culture media at 1 x 10(-7), 1 x 10(-6), 1 x 10(-5) mol xL(-1), separately. 17beta-Estradiol (E2, 1 x 10(-7) mol x L(-7)) was used as positive control. T RAP staining and TRAP activity measurement were performed after 5 days, and the bone resorption pits were analyzed after 7 days. Annexin V staining for the detection of apoptotic osteoclasts was performed after 2, 4, 8, 12, 24, 36 and 48 h separately. The mRNA expression level of TRAP and cathepsin K (CTSK) was measured by real-time RT-PCR. 8-PNG significantly reduced the number of osteoclasts which was TRAP staining positive and with more than three nucleus, the area and number of bone resorption pits decreased obviously in 8-PNG-supplemented groups. The apoptosis rate peaked earlier in the 8-PNG-supplemented groups and the mRNA expression level of TRAP and CTSK decreased significantly. All these inhibitory effects were in a dose dependent manner, the highest effect was obtained by 1 x 10(-5) mol x L(-1) 8-PNG. 8-PNG inhibits bone resorption activity of osteoclasts by inducing osteoclast apoptosis and inhibiting the gene expression and enzyme activity including TRAP and CTSK, and restrains bone marrow cells to osteoclast differentiation.

One hundred and twenty patients with schizophrenia were randomly divided into intervention group and the control group with 60 in each.Family members in intervention group were given community based-rehabilitation training and education once a month for 12 months.Brief Psychiatric Rating Scale (BPRS) and Social Disability Screening Schedule (SDSS) were used to assess the symptoms and social function of patients before and after intervention.The results were compared between two groups.Results showed that the BPRS total score and the SDSS total score in intervention group were lower than those in control group (P ＜ 0.05).The training and education of family members can improve symptoms and functional recovery for patients with schizophrenia.

Objective To investigate the effects of pulsed electromagnetic fields (PEMFs) of different intensities on bone metabolism in ovariectomized rata. Methods Sixty 10-month-old female Sprague-Dawley rats were randomly divided into a normal group, an ovariectomized control group, a 0. 14 mT group, a 0. 16 mT group, a 0.18 mT group and an estrogen group. All rats except those in the normal group were subjected to bilateral ovariectomy. Beginning one week after the operation, the rats in the 0. 14 mT, 0. 16 mT and 0.18 mT groups were treated with PEMFs at 50 Hz, 60 min daily for 90 days, while those in the estrogen group received estrogen instead. During the experiment, the bone mineral density (BMD) of the whole body was observed dynamically, and local BMDs and biochemical indexes were measured after 3 months of treatment. Results Alkaline phosphatase (ALP) activity in the normal group was significantly lower than in the other groups. Tartrate-resistant acidic phosphatase 5b (TRAP5b)activity was elevated significantly, in the control and 0.14 mT groups compared with the normal group. After 2 months of treatment, whole body BMD was reduced significantly in the 0.14 mT group compared to the normal group. After 3 months of treatment, whole body BMD in the ovariectomized controls and the 0.14 mT group was reduced significantly compared to the normal group, but significantly elevated in the 0. 16 mT, 0.18 mT and estrogen groups when compared to the ovariectomized control group. At the end of 3 months of treatment, the trends in lumbar vertebral BMD were similar to those of the whole body BMD, with the femoral BMD in the ovariectomized group and the 0.14 mT group significantly lower than in the normal group, though the differences among the other groups were not statistically significant. Conclusions PEMF treatment at 50 Hz and 0.16 mT or 0.18 mT can promote bone formation. PEMFs at 50 Hz and 0.14 mT might stimulate bone resorption. An intensity-window effect exists in the action of PEMFs on bone metabolism in ovariectomized rats.

A high efficient in vitro regeneration protocol was developed from leaf explants of the female 'Red Sun' kiwifruit (Actinidia chinensis) and the multiplication coefficient and rooting rate of adventitious buds were also optimized. This method does not require formation of callus tissues which leads to somaclonal variations. The results show that the adventitious buds developing directly from explants tissue were noticed after 30 d of culture. The maximum regeneration frequency of adventitious buds is 100% and 18.67 shoots was observed in each leaf explants when MS medium was supplemented with 3.0 mg/L BA+1.0 mg/L NAA. The optimal culture medium for bud multiplication is MS+2.0 mg/L BA+1.0 mg/L NAA+0.1 mg/L GA3 and the multiplication coefficient reached 8.63. On the rooting medium with 1/2 MS+0.8 mg/L IBA for 15 d, the adventitious plantlets were transferred into matrix perlite supplied with 1/2 MS liquid medium for 15 d and the rooting rate reached 100%. 95 out of 98 plantlets (96.94%) survived acclimatization, producing healthy plants in the greenhouse. Taken together, a highly efficient regeneration method via leaf explants of 'Red Sun' kiwifruit was successfully established. This protocol may be useful for micropropagation and genetic transformation studies of 'Red Sun' kiwifruit.
Actinidia ; growth & development ; Plant Growth Regulators ; pharmacology ; Plant Leaves ; growth & development ; Regeneration ; physiology ; Tissue Culture Techniques ; methods

Studying effects of 50 Hz sinusoidal electromagnetic fields (SEMFs) with different intensities on peak bone mass (PBM) of rats may provide a theoretical basis for application of electromagnetic clinical field. 30 female SD rats, 6 weeks of age, were randomly divided into three groups: the control group, 0.1 mT electromagnetic field group (EMFs) and 0.6 mT EMFs. The EMFs groups were treated for 3 h/day. After 8 weeks, we examined their bone mineral densities (BMD) , measured their bone biomechanical properties, and made serum levels of osteocalcin (OC), tartrate-resistant acid phosphatase 5b (TRACP 5b), and histomorphometry. It was found that the BMD (P < 0.01), maximum mechanical load (P < 0.01) in the 0.1 mT group were significantly higher than those in the control group, and Yield strength (P < 0.05), the analyses of serum bone turnover markers and histomorphometric parameters were better than those in the control group (P < 0.05). However, the 0.6 mT group did not have significantly difference comparing with that in the control group. This study proved that 50 Hz 0.1 mT SEMFs can increased BMD, bone strength, and bone tissue microstructure. Therefore, 50 Hz 0.1 mT SEMFs can improve peak bone mass of rats.
Acid Phosphatase ; blood ; Animals ; Bone Density ; Bone and Bones ; physiology ; Electromagnetic Fields ; Female ; Isoenzymes ; blood ; Osteocalcin ; blood ; Rats ; Rats, Sprague-Dawley ; Tartrate-Resistant Acid Phosphatase

Bacterial canker caused by Pseudomonas syringae pv. Actinidiae is one of the most important diseases of kiwifruit (Actinidia chinensis) and leads to considerable yield losses. In order to obtain transgenic plants with resistance for 'Red Sun' kiwifruit to canker disease, a non-specific lipid transfer protein-like antimicrobial protein gene (LJAMP2) from motherwort (Leonurus japonicus) was introduced into 'Red Sun' kiwifruit through Agrobacterium-mediated transformation. After two days of co-cultivation with A. tumefaciens strain LBA4404 harboring 35S:LJAMP2, the transformed explants were transferred to the selection medium containing 25 mg/L kanamycin+3.0 mg/L BA+1.0 mg/L NAA. The regeneration efficiency of kanamycin-resistant shoots reached to 85%. All (100%) of kanamycin-resistant shoots rooted on half-strength MS medium supplemented with 0.8 mg/L IBA and a total of 40 regenerated plantlets were obtained. PCR and histochemical GUS activity analysis show that 23 of 40 lines (57.50%) were positive, suggesting that the LJAMP2 gene was integrated into the genome of 'Red Sun' kiwifruit. Taken together, we established an efficient genetic transformation method for 'Red Sun' kiwifruit using A. tumefaciens and the transformation frequency reached 5.11%. This protocol will be useful for the genetic breeding of 'Red Sun' kiwifruit for improvement of disease resistance.
Actinidia ; genetics ; Agrobacterium ; Antigens, Plant ; genetics ; Carrier Proteins ; genetics ; Leonurus ; Plant Proteins ; genetics ; Plants, Genetically Modified ; genetics ; Transformation, Genetic