Objective To investigate the impact of Ruai-Kangfu decoction on the quality of life of the patients with breast cancer after operation.Methods A total of 107 patients with breast cancer who met the inclusion criteria were randomly divided into the control group (54 patients) and observation group (53 patients). The control group was treated with the radiotherapy and chemotherapy and symptomatic support treatment. The observation group was treated with the Ruai-Kangfu decoction on the basis of the control group. The 2 groups were treated for 12 months, followed up for 6 months. The European Organization for research and treatment of cancer life quality scale (quality of life questionnaire-core 30, QLQ-C30) and the living quality of breast cancer (quality of life questionnaire-breast table cancer module 23, QLQ-BR23) were used for assessing the quality oflife of the patients.Results After treatment, the scores of QLQ-C30 scale the body function (76.4 ± 16.6vs. 70.4 ± 16.4,t=-2.087), role functions (59.2 ± 12.8vs. 54.6 ± 12.8,t=-2.085), emotional function(77.2 ± 17.0 vs. 54.7 ± 12.8,t=-2.075), cognitive function (38.5 ± 14.7 vs. 34.3 ± 11.6t=2.104), social function(59.3 ± 12.4 vs. 54.6 ± 12.8,t=-2.105) and the overall health status (76.7 ± 19.7 vs. 65.5 ± 18.6,t=-2.152) in the observation group were significantly higher than those in the control group (P<0.05). The weary (40.9 ± 8.4 vs. 45.3 ± 9.6, t=2.149), nausea and vomiting (42.9 ± 8.8 vs. 44.9 ± 10.1,t=2.145), pain (36.9 ± 7.6 vs. 40.9 ± 8.7,t=2.143), dyspnea (44.8 ± 9.2 vs. 49.6 ± 10.6,t=2.148), insomnia (41.8 ± 8.6 vs. 46.3 ± 9.9,t=2.152), loss of appetite (35.0 ± 7.2 vs. 38.7 ± 8.2,t=2.113), constipation (15.9 ± 3.3 vs. 17.6 ± 3.8,t=2.057), diarrhea (15.2 ± 3.1 vs. 16.9 ± 3.6, t=2.092) scores in the observation group were significantly lower than those in the control group (P<0.05). The scores of QLQ-BR23 scale body type (74.7 ± 18.5 vs. 64.8 ± 15.4,t=-2.153), the function of sex (36.3 ± 9.0 vs. 42.8 ± 10.2,t=2.045), sexual fun (41.4 ± 10.2 vs. 48.9 ± 11.6,t=2.046), view of the future (67.6 ± 16.7 vs. 58.6 ± 14.0,t=-2.132), side effects (48.2 ± 10.4 vs. 55.2 ± 12.2,t=2.149), breast symptoms (39.6 ± 8.6 vs. 45.5 ± 10.2, t=2.127), arm symptoms (30.3 ± 6.6 vs. 34.8 ± 7.7,t=2.129), trouble from alopecia (36.1 ± 7.8 vs. 41.4 ± 9.1, t=2.113) in the observation group were significantly higher than the control group (P<0.05).Conclusions The Ruai-Kangfu decoction can adjust the mental status and improve the quality of life of patients with breast cancer after operation.

Statins not only can help lower cholesterol,but also has certain effect for the treatment of malignant tumor.Its possible antitumor mechanisms include inducing apoptosis and differentiation,inhibiting tumor cell proliferation,reducing the invasion and metastasis of tumor cells,combined sensitization and chemical prevention effect.

OBJECTIVETo explore the differentially expressed genes and related signaling pathways in MC3T3-E1 osteo- blasts in response to suitable fluid shear stress values and action time with cDNA microarrays.

METHODSMC3T3-E1 cells cultured on a cover slip were subjected to fluid shear stress using a parallel plate flow chamber. The harvested RNA was used for microarray hybridization comprising approximately 44 170 genes, as well as for the subsequent real-time quantitative polymerase chain reaction validation of expression levels for selected genes. Microarray results were analyzed by using both GO and Pathway analysis.

RESULTSMicroarray analysis indicated that 884 differentially expressed genes were found. Among these genes, 444 were upregulated, whereas 440 were downregulated. The Notch signal and RIG- I -like receptor signaling pathways were involved in the Pathway analysis. GO analysis mainly involved different functional classifications, such as prostaglandin biosynthesis, nitric oxide-mediated signal transduction, calcium mediated signal, and cellular immune response, among others.

CONCLUSIONThe mechanism underlying the protective effect of fluid shear stress on MC3T3-E1 cells might be related to promoting cell survival- and inhibiting cell apoptosis-related signaling pathways and biological processes.

Objective The image segmentation of low-density gene chips based on genetic algorithm, which can implement the function of region identification, is achieved. Methods After image denoising by wavelet analysis, image segmentation is accomplished by genetic algorithm. Results The method can detect the region of sampling point more precisely. It can also effectively separate the valuable weak signal points and background or noise. Conclusion The method can accomplish the function of image segmentation of low-density gene chips, which can provide relative accurate data information for future image analysis

Objective To measure the outcomes of the cochlear implantation for patients with Warrensburg syndrome in comparison with non-syndromic patients with sensorineural hearing loss. Methods 16 of 1 300 Waardenburg syndrome patients who received cochlear implants at PUMC hospital were reviewed in this papers. The authors will discuss the effects between Warrensburg syndrome and non- syndromic patients with sensorineural hearing loss by means of the Parents Evaluation of Aural-Oral Performance of Children (PEACH). Results There were no statistical difference in quiet, in noise, in overall results and in conversation on the phone between Warrensburg syndrome and non-syndromic patients. There was statistical difference between in quiet and in noise in every group. Conclusion The auditory perception and speech recognition between Warrensburg syndrome and non-syndromic patients with sensorineural hearing loss is same, and the results in quiet is better than that in noise.

10.3969/j.issn.2095-4344.2013.25.013

In this paper,the significance of developing undergraduates’ creative qualities and how to train undergraduates’ innovative qualities are discussed.The effects of teaching reformation are also analyzed.

Objective To develop a HPLC method for the determination of chlorogenic acid in xiaoaiping injection in human plasma.Methods The analytical column was packed with Hypersil ODS C18 (250 mm×4.6 nm,5 μm).The mobile phase was acetonitrile-0.4% phosphoric acid solution(13:87).The flow rate was 1 ml/min,and detection wave length was set at 327 nm.Results The linear ranges were 2.5～60.0 mg/L chlorogenic acid in human plasma,with the correlation coefficients of 0.9996.The Low limit of detection was 0.25 μg/ml.The method and extraction recovery were 100.1%～102.6%and 78.6%～80.3%.The relative standard deviation value for the within-day and between-day precision were less than 3.9%.Conclusion The method is simple,sensitive for the determination of chlorogenic acid in xiaoaiping injection in human plasma.

[Abstract ] Objective Positive margins of conization specimen for cervical intraepithelial neoplasia grade (CIN)3 is a high risk factor of continuous cervical lesions or progress to early invasive carcinoma .The aim of this study was to evaluate the risk factors of positive margins of conization specimen and post-cone residual disease in hysterectomy specimens following conization for CIN 3. Methods Clinical data from 218 patients with CIN 3 underwent hysterectomy after conization from 2001 to 2013 were analyzed retro-spectivly.Multi-factors logistic regression was performed to determine the independent risk factors for positive margins of conization specimen and post-cone residual disease. Results Fifty five cases(25.2%) had positive margin of conization specimen , and the independent risk factors were unsatisfactory colposcopy (OR:5.18,95%CI:2.49-11.10), width of conization specimen smaller than 2 cm (OR:7.68,95%CI:2.31-28.10) and pathology of CIN3 with carcinoma in situ (OR:4.38,95%CI:1.67 -12.10).Forty seven patients (21.6%) had post-cone residual disease , and the independ-ent risk factors were length of conization specimen smaller than 1.5 cm (OR:2.45,95%CI:1.12-5.69), pathology of CIN3 with carci-noma in situ (OR:4.96,95%CI:2.17-11.70) and status of coniza-tion margins (OR:4.34,95%CI:1.95-9.78). Conclusion The satisfaction of colposcopic examination, conization specimen di-ameters including width and length , CIN3 with carcinoma in situ and status of conization margins could contribute to positive margins of conization or residual disease in subsequent hysterectomy specimens .More attention should be paid to such a group of CIN 3 patients clinically.

As a traditional Chinese medicine,pheretima is widely used for thousands of years in China. According to the studies in recent years,the pharmacological effects and clinical researches of pheretima were summarized based on the introduction of chemical constituents, which could provide information for the further study.