Objective:To pharmacognostically identify Selaginellae uncinatae Herba to provide reference for the identification and utilization of Selaginellae uncinatae Herba. Methods:On the basis of observing the characters, structure and microscopic characteris-tics of the powder, Selaginella uncinata Herba was identified by a TLC method using amentoflavone as the reference substance. The contents of total ash, acid insoluble ash and alcohol-soluble extracts in Selaginellae uncinatae Herba were detected as well. Results:The morphological identification, microscopic identification and TLC identification of the herb was respectively established. The content limits of the total ash, acid insoluble ash and alcohol soluble extract were preliminarily determined. Conclusion:The studies provide reference for the identification and quality evaluation of Selaginellae uncinatae Herba.

OBJECTIVETo explore the differentially expressed genes and related signaling pathways in MC3T3-E1 osteo- blasts in response to suitable fluid shear stress values and action time with cDNA microarrays.

METHODSMC3T3-E1 cells cultured on a cover slip were subjected to fluid shear stress using a parallel plate flow chamber. The harvested RNA was used for microarray hybridization comprising approximately 44 170 genes, as well as for the subsequent real-time quantitative polymerase chain reaction validation of expression levels for selected genes. Microarray results were analyzed by using both GO and Pathway analysis.

RESULTSMicroarray analysis indicated that 884 differentially expressed genes were found. Among these genes, 444 were upregulated, whereas 440 were downregulated. The Notch signal and RIG- I -like receptor signaling pathways were involved in the Pathway analysis. GO analysis mainly involved different functional classifications, such as prostaglandin biosynthesis, nitric oxide-mediated signal transduction, calcium mediated signal, and cellular immune response, among others.

CONCLUSIONThe mechanism underlying the protective effect of fluid shear stress on MC3T3-E1 cells might be related to promoting cell survival- and inhibiting cell apoptosis-related signaling pathways and biological processes.

Apoptosis ; Calcium ; Humans ; Oligonucleotide Array Sequence Analysis ; Osteoblasts ; Signal Transduction ; Stress, Mechanical

OBJECTIVETo explore the suitable level and action time of 17-beta estradiol and fluid shear stress (FSS) and their combined effect on the proliferation of rat osteoblasts in vitro.

METHODSMC3T3-E1 osteoblasts were adopted after subcultured and different concentrations of 17-beta estradiol and FSS values were applied respectively on MC3T3-E1, the suitable level of 17-beta estradiol and FSS were selected through MTT and alkaline phosphatase (ALP). Then the two factors at the suitable level were applied simultaneously to MC3T3-E1 to detect the proliferation activity.

RESULTSSeventeen-beta estradiol(10(-8) mol x L(-1) for 5 d and 12 x 10(-5) N FSS for 60 min exhibited better effects on the proliferation activity than the other groups respectively, and the combined effect of both factors was better than any single-factor treated group.

CONCLUSIONBoth 17-beta estradiol and FSS have a suitable threshold in promoting proliferation of osteoblasts, and two-factor treated group exhibits better effect than any other single-factor treated groups. Therefore 17-beta estradiol and FSS have a synergetic action on differentiation and proliferation of osteoblasts.

Alkaline Phosphatase ; Animals ; Cell Differentiation ; Estradiol ; Osteoblasts ; Rats ; Stress, Mechanical

Objective To explore the differentially expressed genes and related signaling pathways in MC3T3-E1 osteo-blasts in response to suitable fluid shear stress values and action time with cDNA microarrays. Methods MC3T3-E1 cells cultured on a cover slip were subjected to fluid shear stress using a parallel plate flow chamber. The harvested RNA was used for microarray hybridization comprising approximately 44 170 genes, as well as for the subsequent real-time quantitative polymerase chain reaction validation of expression levels for selected genes. Microarray results were analyzed by using both GO and Pathway analysis. Results Microarray analysis indicated that 884 differentially expressed genes were found. Among these genes, 444 were upregulated, whereas 440 were downregulated. The Notch signal and RIG-Ⅰ-like receptor signaling pathways were involved in the Pathway analysis. GO analysis mainly involved different functional classifications, such as prostaglandin biosynthesis, nitric oxide-mediated signal transduction, calcium mediated signal, and cellular immune response, among others. Conclusion The mechanism underlying the protective effect of fluid shear stress on MC3T3-E1 cells might be related to promoting cell survival- and inhibiting cell apoptosis-related signaling pathways and biological processes.

Objective To evaluate the clinical application and nursing effect of implantable venous access ports via the axillary vein.Methods From September 2014 to December 2015,1 263 cases of malignant tumor patients were implanted implantable venous access ports via the axillary vein under ultrasound guidance in Oncology Department of our hospital.The effects of the application of implantation method and nursing were observed.Results The 1 325 cases underwent implantable venous access ports via the axillary vein with 1 263 cases catheterization succeeded,and the success rate is 95.3%.For 62 failed cases,the internal jugular vein catheterization were carried out instead.The average time of implantation time was 10.5 minutes.For the 3 cases of catheter blockage,they were reused after recanalization using thrombolysis under negative pressure with urokinase;for the 2 cases localized infection,the ports were removed and the drug were injected through PICC;no serious complications,namely thrombosis,pinch-off syndrome,catheter disconnection,port body flips et al,happened.Conclusions Implantable venous access ports via the axillary vein with the features of high success ratio,short operating time,can be used as a new selection of port implantation.By establishing the standardization and homogenization of the whole catheter management system postoperatively,strengthening catheter maintenance training for nursing staff,and focusing on the details of the process of operation and maintenance,the complications of infusion port can be reduced,so as to keep the port using for a longer time.

Objective To evaluate the effect of tube sealing using pre-flushed flushing syringes ( flush) and heparin saline on open-ended port for venous transfusion during continuous infusion. Methods During the period of continuous intravenous infusion, a total 90 tumor patients were randomly divided into 2 groups. They used open-ended port for venous transfusion and was sealed by using pre-flushed flushing syringes and 100 U/ml heparin saline respectively. The catheter blockage rate, dispensing time, sealing tube time and cost of single sealing tube were compared between two groups.Results During the period of continuous infusion, the catheter occlusion rate of flush group and the heparin saline group were 1.84% and 1.47% (χ2=0.2642,P>0.05); on the dispensing and seal tube time,they were (11.5±2.9) seconds and (10.5±2.6) seconds in the flash group, and (37.1±2.9) seconds and (24.6±3.3) seconds in the heparin saline group (t=41.982,22.797;P<0.01). The cost for flush sealing is 8.33 yuan lower than that of heparin saline sealing.Conclusions During the period of continuous intravenous infusion, sealing tube by using flush on open-ended infusion port is safe and effective. The workload of the nurses and cost is reduced, it is worth to be promoted.