Objective:To define the localization of aquaporin-4(AQP4)and its mRNA expression in the pancreas.So as to pro- vide morphological basis for the secretory function and regulation mechanisms in the pancreas.Methods:Immunohistochemical (IHC)and in situ hybridization(ISH)staining techniques were used to evaluate the expression of AQP4 and mRNA expression in pancreas.Results:IHC showed that AQP4 and its mRNA were expressed in the membrane of pancreas islet cells;however light-coloured was expressed in the acinus cell.Conclusion:AQP4 might contribute to the precise regulation of water and ion balance in pancreas islet cells,which may be fluctuated during the process of synthesis and secretion of endocrine,but AQP4 might have little contribution to the secretion of pancreatin.

Objective To assess the advantages and disadvantages of urine drainage by double-J ureteral stent or by percutaneous indwelling stent for pyeloplasty. Methods A total of 62 patients who had undergone pyeloplasty (6 patients had undergone operation on both sides) were reviewed.These patients had 68 sides of drainage during operation.The therapeutic effects,relevant complications and recoveries of 31 sides with internal stent and 37 with external stent for pyeloplasty were analyzed retrospectively. Results The rate of total postoperative complications such as hematuria,urinary tract infection,anastomotic leakage of urine or stenosis and urinary salt deposit in the internal drainage group (7/31,22.6%) was remarkably lower than that of the external drainage group (17/37,45.9%), P

Objective To evaluate the effect of simple closure operation followed by anti-Helicobacter pylori(Hp)therapy for perforated peptic ulcer. Methods 168 cases of perforated peptic ulcer treated by simple closure operation with or without postoperative medication therapy were followed-up and analyzed. Results 1 year after operation, the recurrence rate of peptic ulcer was 3.8% in the group of standard anti-Hp therapy(S group), and that of gastric and duodenal ulcer in S group was 8.3% and 3.6%,respectively; while it was 62.9%, 66.7% and 58.8% in the group of non-standard Hp therapy (N-S group), and 88.9%, 100% and 80.0% in the group without Hp therapy (NT group).The diffcrence of reccurrence rate of peptic ulcer between S group with N-S group?NT group was significant(P

Objective: To study the effect of Carisolv in caries removal on dentin bonding surface.Methods:24 extracted third molars with moderate carious were randomly divided into four groups. Each cavity was split into two parts, one part was prepared by Carisolv and the other by high-speed bur. Then samples were processed as follows: samples in group A were not treated, those in group B were treated with 320 ml/L phosphoric acid, SEM was used to observe the morphology of dentin surface of the samples in group A and B. Samples in group C were restored with Dyract AP and those in group D with resin for the observation of the microstructure of dentin-filling bonding interfaces.Results:In group A, rough surface without obvious smear layer was exhibited and dentinal tubules were clearly open after Carisolv preparation; after bur preparation, dentin surfaces were covered with a thick smear layer and no opening of tubules was visible. In group B, after treatment with 320 ml/L phosphoric acid, the smear layer was removed and dentinal tubules were exposed after either Carisolv or bur preparation, but tube stoppers were visible in the samples prepared by bur. In group C, resin tags were observed only in the samples after Carisolv preparation. In group D,dentin surfaces and lengths of the resin tags did not show remarkable difference after Carisolv or bur preparation, but more lateral branch connection between resin tags appeared in the samples prepared by Carisolv than by bur.Conclusion:The dentin prepared by Carisolv favors bonding between dentin and resin/compomer and has the advantage of restoring with compomer.

Objective To evaluate the clinical classification and timing of surgery in the treatment of gallstone acute pancreatitis(GAP). Method The clinical data of 109 patients with GAP admitted to the Department of General Surgery of our hospital were retrospectively analysed. Result and Conclusion Based on the analysis of the treatment methods and its outcome, GAP should be divided into four types according to ampullary obstruction and severity of acute pancreatitis. (1)Non-obstructive mild type GAP was treated mainly in conservative way.(2)Obstructive mild type GAP could be treated conservatively for 36 hours after onset. If the obstruction did not resolve, surgery should be done. (3)Obstructive severe type GAP was treated mainly in conservative way, and the timing of surgery depends on whether necrosis complicated with infection. (4)Obstructive severe type GAP: EST should be done first. If EST is not convenient to be done, an early surgery should be done after short period of supportive therapy. Special attention should be paid to, if suppurative cholecystitis or cholangitis presented, an emergency surgery should be done. Finally, for all the GAP treated by conservative treatment, an elective surgery should be performed to resolve the biliary disease.

Objective To evaluate the changes of GRP78 expression in the Spinal Cord of Ischemia/Reperfusion Injuried Rat.Methods Fifty five adult Wistar rats(250～300 g)were randomly divided into 2 groups as control group(n=5)and operation group(n=50).The spinal cord SCII model was established.The expression of GRP78 was detected in spinal cord tissue through immunohistochemistry(IHC)and Western blot analysis,and the neuronal apoptosis was detected through terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL)methods.Results The GRP78 expression was increased at 30 min of reperfusion,and peaked at 7 h,but began to decline at 11 h post-reperfusion,and reduced significantly at 23 h.The number of GRP78 positive neurons at 0.5,3,7,11 and 23 h groups was(19.4?1.34),(42.6?2.30),(82.4?2.07),(40.0?1.58)and(18.8?0.83),respectively and significantly higher than that of control group(P

Objective To investigate the changes of dopamine-?-hydroxylase(DBH) and activator protein 2-?(AP-2?) expression in spinal cord under the condition of stress or pain stimulation,so as to explore the mechanism for changes of noradrenergic(NA) neurons in the spinal cord of rat pain model.Methods Immunohistochemical staining,double immunofluorescent staining,Western blotting and computing-image analysis system were used to detect the changes of DBH/AP-2? expression in the spinal cord of formalin-induced rat pain model.Results A small number of DBH-positive neurons were sparsely distributed in the ventral horn of the normal spinal cord,while in the formalin-treated group,much more darkly-stained DBH-positive neurons appeared primarily in the ventral horn,intermediate zone,and the dorsal horn,which reached the highest level on day 3 after formalin-injection.The grey value and number of DBH-positive neurons on day 7 after injection began to decrease,but still higher than that in the control group.Compared with control group,the number of noradrenergic neurons in spinal cord of formalin-treated rat was increased significantly,which was also confirmed by Western blotting.Double immunofluorescent staining showed that DBH and AP-2? co-existed in the cells of the spinal cord.The changes of AP-2? expression were similarly to that of DBH in the spinal cord of rat pain model.Conclusion Our results indicated that some non-noradrenergic neurons with different chemical properties might convert into noradrenergic neurons under pain stimulation;noradrenaline may be involved in the formalin-induced pain and behavior regulation;As one of transcription factors,AP-2? may promote the DBH synthesis.

Objective To investigate the association between the ATP-binding cassette transporter A1(ABCA1)gene polymorphisms and plasma high density lipoprotein cholesterol(HDL-C)level in Chinese general population.Methods M883I,R1587K and R219K polymorphisms of the ABCA1 gene were detected by polymerase chain reaction-restriction fragment length polymorphism technique in 376 unrelated Chinese healthy subjects.Results In the study population,the subjects with the KK1587 genotype had markedly lower HDL-C levels than those with RR1587 and RK1587 genotypes(P

Objective To examine the changes of Aβ expression and its related metabolic enzymes in the brains of AD and T2DM mice, so as to explore the possible mechanism of type 2 diabetes mellitus combined with AD.Methods Five-month-old APP/PS1 double transgenic mice, ob/ob mice and the wild-type control mice were employed in this study.Immunohistochemical (IHC) staining, Elisa and Western blot were used to detect SP, Aβ and its related metabolic enzymes.Results A certain number of SPs were observed in the cerebral cortex and hip-pocampus of APP/PS1 mice;SPs were occasionally observed in the cortex of ob/ob mice, while no SP appeared in wild-type mice.Aβ40 and Aβ42 levels were significantly increased in APP/PS1 and ob/ob mice brains as compared with controls (P<0.05), thought both Aβ40 and Aβ42 levels in AD mice were significantly higher than those of ob/ob mice (P<0.05).APP expression level was highest in APP/PS1 mice among 3 groups, and its expressed higher in ob/ob mice than that of control mice (P<0.05).BACE1 expression was notably increased in APP/PS1 and ob/ob mice as compared with control(P<0.05), however, it expressed higher in APP/PS1 mice than ob/ob mice (P<0.05).The expression of Aβ degradation enzyme IDE was reduced in APP/PS1 and ob/ob mice(P<0.05), while lowest in ob/ob mice.Conclusions Overexpression of Aβ may be one of main reasons for T2DM combined AD.