Objective To assess the comparability between different biochemical testing systems.Methods The research ana-lyzed the results of serum enzyme in two biochemical analyzer and figured out the bias estimation by the document of EP9-A2 and explored the comparison of AST、ALT、ALP、LDH and CK in different detected systems.Results It is indicated that in the differ-ent biochemical analysis systems,the comparability of all the indexes could be accepted except for ALP.Conclusion In order to provide a scientific basis for clinic results,comparability test between the methods should be performed and figured out the bias esti-mation while use different detecting systems to monitor the same index.

Bone morphogenetic proteins (BMPs) play a pivotal role in the formation,induction and repair of the bone; BMPs have been confirmed to induce bone formation.Transforming growth factor-beta,insulin-like growth factor-Ⅰ and basic fibroblast growth factor also contribute to bone formation; combination of the factors with BMPs can promote the healing of fracture and non-union,which may have a bright future in orthopedics.

Objective To establish a fluorosis model induced by coal burning and in ameloblasts of rat offsprings.Methods High fluoride air model was established based on the burning coal habit of the epidemic areas.Fluoride feed was made of corn dried by coal burning.Thirty-six SD rats were divided into 3 groups by random number table method according to body weight in a male and female ratio of 2 ∶ 1∶ in the high fluoride air room and rats were feed food with fluorine at 40 mg/kg (high fluoride group),25 mg/kg (low fluoride group);in the normal air room and rats were feed food with fluorine at 0 mg/kg (the control group),12 rats in each group.Mating litter in a ratio of 2 ∶ 1 at the end of 8 weeks.The offsprings were killed on postnatal day 3 and 7 to make mandible sections.Specimens were prepared for light microscope examination to observe the morphological changes of ameloblasts in the tooth germ.Results At the end of 0,2,4,6 and 8 weeks,serum fluoride of the high fluoride group were (0.031 ± 0.003),(0.060 ± 0.006),(0.085 ± 0.006),(0.110 ± 0.007) and (0.134 ± 0.008) mg/L;serum fluoride of the low fluoride group were (0.031 ± 0.003),(0.046 ± 0.005),(0.077 ± 0.006),(0.091 ± 0.007) and (0.104 ± 0.007) mg/L;serum fluoride of the control group were (0.030± 0.003),(0.037 ± 0.002),(0.044 ± 0.002),(0.046 ± 0.003) and (0.049 ± 0.003) mg/L.At the end of 2,4,6 and 8 weeks,serum fluoride of high fluoride group and low fluoride group were significantly higher than that of control group (all P ＜ 0.05).At 7 d,offspring rats in high fluoride group,adamantoblasts were in distortions and vacuole changes,but offspring rats in low fluoride group and the control group had no abnormality.Conclusion By providing rat with high fluoride air and food,we could establish a fluorosis model induced by coal burning in ameloblasts of rat offsprings.

Objective To study the influence of parental generation coal-burning-borne fluorosis on tooth development of their offspring.Methods High fluoride air model was established on the basis of burning coal habit of the epidemic areas.Fluoride feed was made of coal drying corn from the epidemic areas.Totally 48 SD rats were randomly divided into 4 groups with male to female ratio of 1:1 by random number table method.Rats in high,middle and low fluoride groups were put in the high fluoride air room and feed food with 40,25 and 10 mg/kg fluorine,and the control group was put in normal air room and feed normal food.After 8 weeks,rats were mating and parturition.Tooth eruption time of offspring rat was observed;and dental fluorosis incidence,the tooth length and fluorine content were observed at 21 d.Results In high and middle fluoride groups [(6.83 ± 0.94),(6.25 ± 1.06) d],tooth eruption time of offspring rat was later than that of control group [(5.34 ± 0.89) d,all P ＜ 0.01].At 21 d,dental fluorosis was observed in the lower incisors of the high and middle fluorine groups;compared with control group [(5.21 ± 0.19) mm,(223.00 ± 14.08) μg/kg],the tooth length was decreased [(4.83 ± 0.22),(4.96 ± 0.25) mm,P ＜ 0.01or ＜ 0.05],and tooth fluoride content was increased [(362.64 ± 20.35),(289.79 ± 19.18) μg/kg,all P ＜ 0.01].Dental fluorosis incidence of offspring rats was positively correlated with the fluorine dose (r =0.704,P ＜ 0.01).Conclusion Parental generation rats’ intaking excessive fluoride can affect offspring rats tooth development and dental fluorosis,which is related to the fluorine dose.

Objective To investigate the effects of crude extracted proteins from lesions of condyloma acuminata on immunophenotypes of dendritic cells.Methods Plastic-adherent mononuclear cells(MNCs)were isolated from umbilical cord blood or peripheral blood and cultured in media containing cytokines(GM-CSF,IL-4and LPS).The morphology and phenotypes of these cells were analyzed by flow cytometry and microscopy in12day's culture.Cells on the fourth day were incubated with crude extracts from lesions of condyloma acuminata,foreskin proteins,and PBS,respectively,followed by phenotypic analysis after9-12days' culture.Results Expression of antigens CD1a,CD80,CD86,MHC-I,MHC-II,CD14,CD54was detected after12days' cul-ture.It was shown that MNCs could be induced to differentiate to mature dendritic cells in our culture system.After incubation with crude extracts from lesions of condyloma acuminata for another9-12days,expression of CD86and HLA-DR was increased on dendritic cells.Conclusions Compared to foreskin and PBS pulsed dendritic cells,expression of CD86and HLA-DR is upregulated on dentritic cells after pulsing with condyloma acuminata lesion proteins.The data suggest that crude extracts from lesions of condyloma acuminata might enhance antigen-pre-senting capacity of dendritic cells and strengthen activation of T lymphocytes.

Objective To study the relationship between single nucleotide polymorphisms of MMP1 gene (-1607) and cyclosporine-induced gingival overgrowth ( CsA-GO ) . Methods Polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) were used to detect 42 cases of cyclosporine-induced gingival hyperplasia patients and 118 normal controls. MMP1 (-1607) 1G/2G gene polymorphism type were detected and analysised;Logistic regres-sion model was used to calculate the different genotypes and the risk of cyclosporine-induced gingival hyperplasia re-lationship. Results Cyclosporine-induced MMP1 (-1607) 3 genotype frequency distribution of gingival hyperplasiacase group had no significant difference (P=0.37) with the control group. Patients who carry MMP1 (-1607) 2G genotype occurred cyclosporine-induced gingival hyperplasia have 1.37 times risk than 1G genotype (95%CI=0.81-2.36, P=0.24), and 1G genotype and cyclosporine-inducedgingival hyperplasiaun related torisk. Conclusion MMP1 gene promoter region (-1607) 1G/2G single nucleotide polymorphism of cyclosporine -induced gingival hyperplasia may not a genetic susceptibility factor, patients who carry 2G genotype has an increased risk of CsA-GO.

Objective:To explore the protective mechanism of tea polyphenols (TP) on mouse oral cancer.Methods:A total of 50 mice were divided into control group, model group, TP group, Selisistat group, TP+ Selisistat group, with 10 mice in each group. The control group was gavaged with physiological saline, while the model group, TP group, Selisistat group, and TP+ Selisistat group were gavaged with 300 mg/L 4-NQO to establish a mouse oral cancer model. Physiological saline, 200 mg/kg TP, 0.01 mg/kg Selisistat, and 200 mg/kg TP+ 0.01 mg/kg Selisistat were gavaged respectively. The weight changes of each group of mice were compared; HE staining was used to observe the morphology of mouse oral tumor tissue; Enzyme linked immunosorbent assay was used to detect the levels of malondialdehyde (MDA) and superoxide dismutase (SOD) in serum; Immunoblotting and immunohistochemistry were used to detect the expression of silencing information regulatory factor (Sirt1) and nuclear factor E2 related factor 2 (Nrf2) proteins in mouse oral tissues.Results:Compared with the control group, the model group mice had a decrease in body weight [(23.19±1.36)g], a decrease in serum SOD level [(91.64±8.75)U/ml], an increase in MDA level [(5.18±0.46)nmol/ml], a decrease in Sirt1 (0.38±0.05) and Nrf2 (0.36±0.05) protein expression in oral tissue, and an increase in Nrf2 acetylation level (0.84±0.11) (all P<0.05). Compared with the model group, the TP group mice had an increase in body weight [(25.28±1.25)g], elevated serum SOD levels [(121.24±10.68)U/ml], decreased MDA levels [(3.89±0.42)nmol/ml], increased expression of Sirt1 (0.61±0.09) and Nrf2 (0.58±0.06) proteins in oral tissue, and decreased Nrf2 protein acetylation levels (0.39±0.05); The Selisistat group mice showed a decrease in body weight [(21.41±1.07)g], a decrease in serum SOD levels [(72.16±7.43)U/ml], an increase in MDA levels [(5.87±0.41)nmol/ml], a decrease in Sirt1 (0.23±0.04) and Nrf2 protein (0.24±0.03) expression in oral tissue, and an increase in Nrf2 acetylation levels (1.12±0.14) ( P<0.05). The body weight [(23.32±1.27)g], serum SOD levels [(92.58±8.13)U/ml], and oral Sirt1 (0.41±0.06) and Nrf2 (0.38±0.05) protein expression in the TP+ Selisistat group mice were higher than those in the Selisistat group, while MDA [(5.11±0.38)nmol/ml] and Nrf2 acetylation levels (0.82±0.09) were lower than those in the Selisistat group (all P<0.05). Conclusions:Tea polyphenols can alleviate oral tissue damage and alleviate oxidative stress in mice with oral cancer, and their mechanism may be related to the upregulation of the Sirt1/Nrf2 pathway.

Artificial hearts(blood pumps)exhibit excellent hydraulic performance and compatibility with blood.Improvement in blood pump performance depends on the design and optimization method.This review summarizes relevant work on designing and optimizing centrifugal blood pumps.First,the progress of numerical simulation methods is reviewed.High-fidelity flow field numerical simulations are prerequisites for design and optimization.The parameter sensitivity studies are summarized mainly from the aspects of the impeller,volute,and clearances.Several commonly used design and optimization methods have been summarized,including parameter sensitivity research,orthogonal optimization,machine learning/genetic algorithms,and topology optimization.Finally,the prospects and challenges for designing and optimizing blood pumps are discussed.This review provides valuable references and guidance for the future design and optimization of blood pumps.

Objective:To explore the effect of health education based on action research theory and swallowing rehabilitation nursing for stroke patients with dysphagia.Methods:The random sampling method was used to select 128 stroke patients with dysphagia who were admitted to China-Japan Union Hospital of Jilin University from November 2020 to November 2021. According to the random number table method, they were divided into the control group and the experimental group. The control group received swallowing rehabilitation nursing, while the experimental group received action research theory health education on the basis of the control group. The swallowing function, self-care ability and quality of life were compared between the two groups.Results:Before nursing, there was no statistically significant difference in scores of Standardized Swallowing Assessment (SSA) , Exercise of Self-Care Agency (ESCA) and Swallowing-Quality of Life (SWAL-QOL) between the two groups ( P>0.05) . After 3 months of nursing, the SSA score of the two groups was lower than that before nursing, and the experimental group was lower than that of the control group, and the differences were statistically significant ( P<0.05) . The scores of each dimension of ESCA scale in the two groups were higher than those before nursing and the experimental group were higher than the control group, and the differences were statistically significant ( P<0.05) . The scores of each dimension of SWAL-QOL scale in the two groups were higher than those before nursing, and the experimental group were higher than the control group, and the differences were statistically significant ( P<0.05) . Conclusions:The application of action research theory health education combined with swallowing rehabilitation nursing in stroke patients with dysphagia can improve the swallowing function of patients, self-care ability and quality of life.

The clinical characteristics of the pneumonia includes hyperthermia, cough and pectoralgia, etc with simultaneous mixed signs of inflammatory infiltration, consolidation, cavity/air sac and abscess in pulmonary CT scan, and these signs change rapidly. Respiratory failure and septic shock frequently occur in severe such patients, resulting in refractory management and relatively long therapeutic course. The timely diagnosis, use of sensitive antibiotics, respiratory and nutritional support, etc comprehensive effective measures can elevate the rescue success rate with severe hematogenous staphylococcus aureus pneumonia.