1.Effect of IL-15 addition on asbestos-induced suppression of human cytotoxic T lymphocyte induction.
Naoko KUMAGAI-TAKEI ; Yasumitsu NISHIMURA ; Hidenori MATSUZAKI ; Suni LEE ; Kei YOSHITOME ; Tatsuo ITO ; Takemi OTSUKI
Environmental Health and Preventive Medicine 2021;26(1):50-50
BACKGROUND:
Asbestos fibers possess tumorigenicity and are thought to cause mesothelioma. We have previously reported that exposure to asbestos fibers causes a reduction in antitumor immunity. Asbestos exposure in the mixed lymphocyte reaction (MLR) showed suppressed induction of cytotoxic T lymphocytes (CTLs), accompanied by a decrease in proliferation of CD8
METHODS:
For MLR, human peripheral blood mononuclear cells (PBMCs) were cultured with irradiated allogenic PBMCs upon exposure to chrysotile B asbestos at 5 μg/ml for 7 days. After 2 days of culture, IL-15 was added at 1 ng/ml. After 7 days of MLR, PBMCs were collected and analyzed for phenotypic and functional markers of CD8
RESULTS:
IL-15 addition partially reversed the decrease in CD3
CONCLUSION
These findings indicate that CTLs induced upon exposure to asbestos possess dysfunctional machinery that can be partly compensated by IL-15 supplementation, and that IL-15 is more effective in the recovery of proliferation and granzyme B levels from asbestos-induced suppression of CTL induction compared with IL-2.
Asbestos/adverse effects*
;
CD8-Positive T-Lymphocytes/metabolism*
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Humans
;
Interleukin-15/pharmacology*
;
Lymphocyte Activation/immunology*
;
T-Lymphocytes, Cytotoxic/metabolism*
2.Effect of asbestos exposure on differentiation and function of cytotoxic T lymphocytes.
Naoko KUMAGAI-TAKEI ; Yasumitsu NISHIMURA ; Megumi MAEDA ; Hiroaki HAYASHI ; Hidenori MATSUZAKI ; Suni LEE ; Kei YOSHITOME ; Tatsuo ITO ; Takemi OTSUKI
Environmental Health and Preventive Medicine 2020;25(1):59-59
Asbestos exposure is known to cause malignant mesothelioma, which is associated with poor prognosis. We focused on and examined the effect of asbestos exposure on the differentiation and function of cytotoxic T lymphocytes (CTLs). CTLs have the ability to specifically attack tumor cells after being differentiated from naïve CD8 T cells following antigen stimulation. Exposure to chrysotile B asbestos suppressed the differentiation of CTLs during the mixed lymphocyte reaction (MLR) and was associated with a decrease in proliferation of CD8 T cells. Additionally, in an effort to investigate the mechanism associated with suppressed CTL differentiation upon exposure to asbestos, we focused on IL-2, a cytokine involved in T cell proliferation. Our findings indicated that insufficient levels of IL-2 are not the main cause for the suppressed induction of CTLs by asbestos exposure, although they suggest potential improvement in the suppressed CTL function. Furthermore, the functional properties of peripheral blood CD8 lymphocytes from asbestos-exposed individuals with pleural plaque (PP) and patients with malignant mesothelioma (MM) were examined. MM patients showed lower perforin levels in CD8 lymphocytes following stimulation compared with PP-positive individuals. The production capacity of IFN-γ in the MM group tended to be lower compared with healthy volunteers or PP-positive individuals. In an effort to determine whether chronic and direct asbestos exposure affected the function of CD8 T cells, cultured human CD8 T cells were employed as an in vitro model and subjected to long-term exposure to chrysotile (CH) asbestos. This resulted in decreased levels of intracellular perforin and secreted IFN-γ. Those findings underlie the possibility that impaired CD8 lymphocyte function is caused by asbestos exposure, which fail to suppress the development of MM. Our studies therefore reveal novel effects of asbestos exposure on CTLs, which might contribute towards the development and implementation of an effective strategy for the prevention and cure of malignant mesothelioma.
3.Decrease in Serum Amyloid a Protein Levels Following Three-month Stays in Negatively Charged Particle-dominant Indoor Air Conditions.
Suni LEE ; Shoko YAMAMOTO ; Yasumitsu NISHIMURA ; Hidenori MATSUZAKI ; Kei YOSHITOME ; Tamayo HATAYAMA ; Miho IKEDA ; Min YU ; Nagisa SADA ; Naoko KUMAGAI-TAKEI ; Takemi OTSUKI
Biomedical and Environmental Sciences 2018;31(5):335-342
OBJECTIVEThe changes in serum adipokines and cytokines related to oxidative stress were examined during 3 months 'Off to On' and 'On to Off' periods using negatively charged particle-dominant indoor air conditions (NCPDIAC).
METHODSSeven volunteers participated in the study, which included 'OFF to 3 months ON' periods (ON trials) for a total of 16 times, and 'ON to 3 months OFF' (OFF trials) periods for a total of 13 times.
RESULTSWith the exception of one case, serum amyloid A (SAA) levels decreased significantly during the ON trials.
CONCLUSIONConsidering that SAA is an acute phase reactive protein such as C reactive protein (CRP), this observed decrease might indicate the prevention of cardiovascular and atherosclerotic changes, since an increase in high-sensitive CRP is associated with the subsequent detection of these events.
Adult ; Air ; analysis ; Air Pollution, Indoor ; Environmental Monitoring ; Female ; Housing ; Humans ; Male ; Serum Amyloid A Protein ; metabolism
4.Biological Effects of Cloth Containing Specific Ore Powder in Patients with Pollen Allergy.
Suni LEE ; Hitoshi OKAMOTO ; Shoko YAMAMOTO ; Tamayo HATAYAMA ; Hidenori MATSUZAKI ; Naoko KUMAGAI-TAKEI ; Kei YOSHITOME ; Yasumitsu NISHIMURA ; Toshiaki SATO ; Yasuzo KIRITA ; Yoshio FUJII ; Takemi OTSUKI
Biomedical and Environmental Sciences 2016;29(8):563-573
OBJECTIVEThe custom-homebuilding company, Cosmic Garden Co. Ltd., located in Okayama City, Japan was established in 1997 and uses specific natural ore powder (SNOP) in wall materials and surveys customers in order to improve allergic symptoms.
METHODSTo investigate the biological effects of SNOP, patients with a pollen allergy were recruited to stay in a room surrounded by cloth containing SNOP (CCSNOP), and their symptoms and various biological parameters were compared with those of individuals staying in a room surrounded by control non-woven cloth (NWC). Each stay lasted 60 min. Before and immediately after the stay, a questionnaire regarding allergic symptoms, as well as POMS (Profile of Mood Status) and blood sampling, was performed. Post-stay minus pre-stay values were calculated and compared between CCSNOP and NWC groups.
RESULTSResults indicated that some symptoms, such as nasal obstruction and lacrimation, improved, and POMS evaluation showed that patients were calmer following a stay in CCSNOP. Relative eosinophils, non-specific Ig E, epidermal growth factor, monocyte chemotactic protein-1, and tumor necrosis factor-α increased following a stay in CCSNOP.
CONCLUSIONThis ore powder improved allergic symptoms, and long-term monitoring involving 1 to 2 months may be necessary to fully explore the biological and physical effects of SNOP on allergic patients.
Adult ; Chemokine CCL2 ; immunology ; Clothing ; Female ; Geologic Sediments ; chemistry ; Humans ; Immunoglobulin E ; immunology ; Japan ; Male ; Pollen ; immunology ; Rhinitis, Allergic, Seasonal ; immunology ; therapy ; Tumor Necrosis Factor-alpha ; immunology