ObjectiveTo explore the pharmacological mechanism involved in the treatment of allergic cough （AC） by Xiaoer Huatan Zhike granules （XEHT） based on proteomics and network pharmacology. MethodsAfter sensitization by intraperitoneal injection of 1 mL suspension containing 2 mg ovalbumin （OVA） and 100 mg aluminum hydroxide， a guinea pig model of allergic cough was constructed by nebulization with 1% OVA. The modeled guinea pigs were randomized into the model， low-， medium- and high-dose （1， 5， 20 g·kg^-1， respectively） XEHT， and sodium montelukast （1 mg·kg^-1） groups （n=6）， and another 6 guinea pigs were selected as the blank group. The guinea pigs in drug administration groups were administrated with the corresponding drugs by gavage， and those in the blank and model groups received the same volume of normal saline by gavage， 1 time·d^-1. After 10 consecutive days of drug administration， the guinea pigs were stimulated by 1% OVA nebulization， and the coughs were observed. The pathological changes in the lung tissue were observed by hematoxylin-eosin staining. The enzyme-linked immunosorbent assay was performed to measure the levels of C-reactive protein （CRP）， interleukin-6 （IL-6）， tumor necrosis factor-α （TNF-α）， superoxide dismutase （SOD）， and malondialdehyde （MDA） in the bronchoalveolar lavage fluid （BALF） and immunoglobulin G （IgG） and immunoglobulin A （IgA） in the serum. Immunohistochemistry （IHC） was employed to observe the expression of IL-6 and TNF-α in the lung tissue. Transmission electron microscopy was employed observe the alveolar type Ⅱ epithelial cell ultrastructure. Real-time PCR was employed to determine the mRNA levels of IL-6， interleukin-1β （IL-1β）， and TNF-α in the lung tissue. Label-free proteomics was used to detect the differential proteins among groups. Network pharmacology was used to predict the targets of XEHT in treating AC. The Kyoto Encyclopedia of Genes and Genomes （KEGG） enrichment analysis was performed to search for the same pathways from the results of proteomics and network pharmacology. ResultsCompared with the blank group， the model group showed increased coughs （P<0.01）， elevated levels of CRP， TNF-α， IL-6， and MDA and lowered level of SOD in the BALF （P<0.05， P<0.01）， elevated levels of IgA and IgG in the serum （P<0.05， P<0.01）， congestion of the lung tissue and infiltration of inflammatory cells， increased expression of IL-6 and TNF-α （P<0.01）， large areas of low electron density edema in type Ⅱ epithelial cells， obvious swelling and vacuolization of the organelles， karyopyknosis or sparse and dissolved chromatin， and up-regulated mRNA levels of IL-6， IL-1β， and TNF-α （P<0.01）. Compared with the model group， the drug administration groups showed reduced coughs （P<0.01）， lowered levels of CRP， TNF-α， IL-6， and MDA and elevated level of SOD in the BALF （P<0.05， P<0.01）， alleviated lung tissue congestion， inflammatory cell infiltration， and type Ⅱ epithelial cell injury， and decreased expression of IL-6 and TNF-α （P<0.01）. In addition， the medium-dose XEHT group and the montelukast sodium group showcased lowered serum levels of IgA and IgG （P<0.05， P<0.01）. The medium- and high-dose XEHT groups and the montelukast sodium showed down-regulated mRNA levels of IL-6， IL-1β， and TNF-α and the low-dose XEHT group showed down-regulated mRNA levels of IL-6 and TNF-α （P<0.05， P<0.01）. Phospholipase D， mammalian target of rapamycin （mTOR）， and epidermal growth factor receptor family of receptor tyrosine kinase （ErbB） signaling pathways were the common pathways predicted by both proteomics and network pharmacology. ConclusionProteomics combined with network pharmacology reveal that XEHT can ameliorate AC by regulating the phospholipase D， mTOR， and ErbB signaling pathways.

Hypoxemia is a common pathological state characterized by low oxygen saturation in the blood. This condition compromises mucosal barrier integrity particularly in the gut and oral cavity. However, the mechanisms underlying this association remain unclear. This study used periodontitis as a model to investigate the role of platelet activation in oral mucosal immunopathology under hypoxic conditions. Hypoxia upregulated methyltransferase-like protein 4 (METTL4) expression in platelets, resulting in N⁶-methyladenine modification of mitochondrial DNA (mtDNA). This modification impaired mitochondrial transcriptional factor A-dependent cytosolic mtDNA degradation, leading to cytosolic mtDNA accumulation. Excess cytosolic mt-DNA aberrantly activated the cGAS-STING pathway in platelets. This resulted in excessive platelet activation and neutrophil extracellular trap formation that ultimately exacerbated periodontitis. Targeting platelet METTL4 and its downstream pathways offers a potential strategy for managing oral mucosa immunopathology. Further research is needed to examine its broader implications for mucosal inflammation under hypoxic conditions.
DNA, Mitochondrial/metabolism* ; Mouth Mucosa/pathology* ; Hypoxia/immunology* ; Methyltransferases/metabolism* ; Blood Platelets/metabolism* ; Animals ; Periodontitis/immunology* ; Humans ; Platelet Activation ; Mice

Objective To explore and analyse the factors influencing the acceptability of"Internet medical alliance"among medical staff by UTAUT model,with a view to providing a basis for the sustainable development of"Internet medical alliance".Methods A questionnaire survey was conducted among medical staff participating in the"Internet medical alliance"in municipal hospitals,count-level hospitals,township health centers,village clinics and community health service centers.SPSS 20.0 and Amos 17.0 software were used to statistically describe the acceptance of"Internet medical alliance",and a structural equation model with behavioral intention and satisfaction as dependent variables was constructed.Results In the range of independent variables,the 841 follow-up subjects had the highest individual creativity score at(3.88±0.67),followed by social influence at(3.86±0.66)and the lowest perceived risk score at(3.52±0.78).Of the dependent variables,the behavioural intention score was(3.86±0.68)and the satisfaction score was(7.72±1.88).Conclusion Facilitation,individual creativity,self-efficacy,performance expectations,effort expectations,having heard of"Internet medical alliance"education level and perceived risk were key factors in the behavioral intention and satisfaction of medical staff to participate in"Internet medical alliance".

During the complete denture restoration process, accurate impression making is a crucial step for achieving good denture retention. With the increasing popularity of intraoral scanning technology in fixed restoration, the use of intraoral scanning technology in complete denture restoration for edentulous jaw has also been developed. This article systematically reviews the research progress and application of intraoral scanning of edentulous soft tissue, focusing on difficulties in intraoral scanning of edentulous jaws, scanning accuracy, clinical application effects, as well as precautions involved. The aim is to provide references for clinical application.

The National Conference on Medical Emergency Work in 2024 clearly puts forward the need to continu-ously strengthen the construction of emergency medical rescue system and capacity,does a good job in medical treatment of emergencies,and achieves high-quality development of medical emergency work.On the basis of ana-lysing the current situation of medical emergency rescue system construction,taking the construction practice of the first emergency hospital in China in Guangdong Second People's Hospital as an example,it explores the construc-tion of a new model of medical emergency rescue in large public hospitals,including three kinds of capabilities:the ability to transform the combination of level and emergency,the ability to integrate regional medical rescue and coor-dination,and three-dimensional multifunctional emergency rescue capabilities;and two norms:norms for talent training and norms for the management system.Finally,it puts forward suggestions for strengthening the construc-tion of emergency hospitals.

The National Conference on Medical Emergency Work in 2024 clearly puts forward the need to continu-ously strengthen the construction of emergency medical rescue system and capacity,does a good job in medical treatment of emergencies,and achieves high-quality development of medical emergency work.On the basis of ana-lysing the current situation of medical emergency rescue system construction,taking the construction practice of the first emergency hospital in China in Guangdong Second People's Hospital as an example,it explores the construc-tion of a new model of medical emergency rescue in large public hospitals,including three kinds of capabilities:the ability to transform the combination of level and emergency,the ability to integrate regional medical rescue and coor-dination,and three-dimensional multifunctional emergency rescue capabilities;and two norms:norms for talent training and norms for the management system.Finally,it puts forward suggestions for strengthening the construc-tion of emergency hospitals.

The National Conference on Medical Emergency Work in 2024 clearly puts forward the need to continu-ously strengthen the construction of emergency medical rescue system and capacity,does a good job in medical treatment of emergencies,and achieves high-quality development of medical emergency work.On the basis of ana-lysing the current situation of medical emergency rescue system construction,taking the construction practice of the first emergency hospital in China in Guangdong Second People's Hospital as an example,it explores the construc-tion of a new model of medical emergency rescue in large public hospitals,including three kinds of capabilities:the ability to transform the combination of level and emergency,the ability to integrate regional medical rescue and coor-dination,and three-dimensional multifunctional emergency rescue capabilities;and two norms:norms for talent training and norms for the management system.Finally,it puts forward suggestions for strengthening the construc-tion of emergency hospitals.

The National Conference on Medical Emergency Work in 2024 clearly puts forward the need to continu-ously strengthen the construction of emergency medical rescue system and capacity,does a good job in medical treatment of emergencies,and achieves high-quality development of medical emergency work.On the basis of ana-lysing the current situation of medical emergency rescue system construction,taking the construction practice of the first emergency hospital in China in Guangdong Second People's Hospital as an example,it explores the construc-tion of a new model of medical emergency rescue in large public hospitals,including three kinds of capabilities:the ability to transform the combination of level and emergency,the ability to integrate regional medical rescue and coor-dination,and three-dimensional multifunctional emergency rescue capabilities;and two norms:norms for talent training and norms for the management system.Finally,it puts forward suggestions for strengthening the construc-tion of emergency hospitals.

The National Conference on Medical Emergency Work in 2024 clearly puts forward the need to continu-ously strengthen the construction of emergency medical rescue system and capacity,does a good job in medical treatment of emergencies,and achieves high-quality development of medical emergency work.On the basis of ana-lysing the current situation of medical emergency rescue system construction,taking the construction practice of the first emergency hospital in China in Guangdong Second People's Hospital as an example,it explores the construc-tion of a new model of medical emergency rescue in large public hospitals,including three kinds of capabilities:the ability to transform the combination of level and emergency,the ability to integrate regional medical rescue and coor-dination,and three-dimensional multifunctional emergency rescue capabilities;and two norms:norms for talent training and norms for the management system.Finally,it puts forward suggestions for strengthening the construc-tion of emergency hospitals.

The National Conference on Medical Emergency Work in 2024 clearly puts forward the need to continu-ously strengthen the construction of emergency medical rescue system and capacity,does a good job in medical treatment of emergencies,and achieves high-quality development of medical emergency work.On the basis of ana-lysing the current situation of medical emergency rescue system construction,taking the construction practice of the first emergency hospital in China in Guangdong Second People's Hospital as an example,it explores the construc-tion of a new model of medical emergency rescue in large public hospitals,including three kinds of capabilities:the ability to transform the combination of level and emergency,the ability to integrate regional medical rescue and coor-dination,and three-dimensional multifunctional emergency rescue capabilities;and two norms:norms for talent training and norms for the management system.Finally,it puts forward suggestions for strengthening the construc-tion of emergency hospitals.