Objective:To discuss the dynamic three-dimensional reconstruction of tissue Doppler ultrasound cardiac image.Methods: To separate anatomical structure and function parameter information from Doppler ultrasound medical image, and then combines with the three-dimensional distribution to reconstruct it and fuse imaging.Results: Ultimately, it reveals the relationship between function parameter and anatomical structure. The dynamic three-dimensional reconstruction of tissue Doppler ultrasound cardiac image is also expounded in this paper.Conclusion: It scientifically completed the dynamic three-dimensional reconstruction of tissue Doppler ultrasound cardiac image by combining both tissue Doppler imaging and ultrasound medical image reconstruction technique. It is of great significance in clinical medicine.

Objective To construct lentivirus vectors carrying 16 short hairpin RNA (shRNA) expression cassettes targeting histone acetyltransferases and provide a powerful research approach to explore the mechanism of epigenetic genes in regulating hepatitis B virus (HBV).Methods Following the rule of short shRNA primer design,eight-pair primers (A ~ H )for each gene,which had stable interfering efficiency,were designed.The annealed primers were connected to the empty lentiviral vectors of shRNA for transformation.In order to confirm the positive clones,clones were analyzed by real-time polymerase chain reaction (RT-PCR ).Then, qualified plasmids were verified by enzyme digestion technology.Four shRNA lentivirus plasmids against the same gene were mixed to build lentivirus respectively.After the virus transfected into 293T cells for 48 and 72 hours,supernatants were collected to infect HepG2.2.15 cells.The percentage of fluorescent cells were observed and assessed by microscope 72 hours after infection.Results One hundred and twenty-eight lentiviral vectors of RNA interference (RNAi)library were constructed against 16 histone acetyltransferases and more than 80% of HepG2.2.15 cells were infected with lentivirus 72 hours after infection.Conclusions Sixteen shRNA lentivirus vectors against histone acetyltransferase are successfully constructed.Thus,a solid foundation for the study of the effect of human histone deacetylase on HBV replication is established.

OBJECTIVE:To establish a method for the content determination of polymer in cefatrizine propylene glycol. METHODS:High performance sephadex gel chromatography was performed on the column of Sephadex G-10 with mobile phase A of 0.01 mol/L phosphate buffer [0.01 mol/L Disodium hydrogen phosphate solution-0.01 mol/L Sodium dihydrogen phosphate solution (61∶39,V/V)](pH7.0)and mobile phase B of water at a flow rate of 1.0 ml/min,the detection wavelength was 254 nm,column temperature was 30℃,and volume injection was 200μl. RESULTS:The linear range of polymer was 2.07-103.30 mg/ml(r=0.999 4);the limit of quantitation of 10.4 ng,limit of detection was 4.1 ng;RSDs of precision and reprodicibility tests were lower than 3%. CONCLUSIONS:The method is specific with high sensitivity and good reproducibility,and can be used for the content determination of polymer in active pharmacentical ingredient cefatrizine propylene glycol.

The National Conference on Medical Emergency Work in 2024 clearly puts forward the need to continu-ously strengthen the construction of emergency medical rescue system and capacity,does a good job in medical treatment of emergencies,and achieves high-quality development of medical emergency work.On the basis of ana-lysing the current situation of medical emergency rescue system construction,taking the construction practice of the first emergency hospital in China in Guangdong Second People's Hospital as an example,it explores the construc-tion of a new model of medical emergency rescue in large public hospitals,including three kinds of capabilities:the ability to transform the combination of level and emergency,the ability to integrate regional medical rescue and coor-dination,and three-dimensional multifunctional emergency rescue capabilities;and two norms:norms for talent training and norms for the management system.Finally,it puts forward suggestions for strengthening the construc-tion of emergency hospitals.

The development of targeted oral drugs that can stably treat inflammatory bowel disease (IBD) is still a clinical problem to be solved. In recent years, studies have confirmed that sphingosine⁃1⁃phosphate (S1P)/S1P receptor pathway can regulate lymphocyte homing and immune regulation, inhibit intestinal inflammation, protect intestinal endothelial barrier, and affect intestinal microbial metabolism, which may play a key role in the treatment of IBD. This article reviewed the effect of S1P/S1P receptor pathway on IBD and its potential mechanism.

The National Conference on Medical Emergency Work in 2024 clearly puts forward the need to continu-ously strengthen the construction of emergency medical rescue system and capacity,does a good job in medical treatment of emergencies,and achieves high-quality development of medical emergency work.On the basis of ana-lysing the current situation of medical emergency rescue system construction,taking the construction practice of the first emergency hospital in China in Guangdong Second People's Hospital as an example,it explores the construc-tion of a new model of medical emergency rescue in large public hospitals,including three kinds of capabilities:the ability to transform the combination of level and emergency,the ability to integrate regional medical rescue and coor-dination,and three-dimensional multifunctional emergency rescue capabilities;and two norms:norms for talent training and norms for the management system.Finally,it puts forward suggestions for strengthening the construc-tion of emergency hospitals.

The National Conference on Medical Emergency Work in 2024 clearly puts forward the need to continu-ously strengthen the construction of emergency medical rescue system and capacity,does a good job in medical treatment of emergencies,and achieves high-quality development of medical emergency work.On the basis of ana-lysing the current situation of medical emergency rescue system construction,taking the construction practice of the first emergency hospital in China in Guangdong Second People's Hospital as an example,it explores the construc-tion of a new model of medical emergency rescue in large public hospitals,including three kinds of capabilities:the ability to transform the combination of level and emergency,the ability to integrate regional medical rescue and coor-dination,and three-dimensional multifunctional emergency rescue capabilities;and two norms:norms for talent training and norms for the management system.Finally,it puts forward suggestions for strengthening the construc-tion of emergency hospitals.

The National Conference on Medical Emergency Work in 2024 clearly puts forward the need to continu-ously strengthen the construction of emergency medical rescue system and capacity,does a good job in medical treatment of emergencies,and achieves high-quality development of medical emergency work.On the basis of ana-lysing the current situation of medical emergency rescue system construction,taking the construction practice of the first emergency hospital in China in Guangdong Second People's Hospital as an example,it explores the construc-tion of a new model of medical emergency rescue in large public hospitals,including three kinds of capabilities:the ability to transform the combination of level and emergency,the ability to integrate regional medical rescue and coor-dination,and three-dimensional multifunctional emergency rescue capabilities;and two norms:norms for talent training and norms for the management system.Finally,it puts forward suggestions for strengthening the construc-tion of emergency hospitals.

The National Conference on Medical Emergency Work in 2024 clearly puts forward the need to continu-ously strengthen the construction of emergency medical rescue system and capacity,does a good job in medical treatment of emergencies,and achieves high-quality development of medical emergency work.On the basis of ana-lysing the current situation of medical emergency rescue system construction,taking the construction practice of the first emergency hospital in China in Guangdong Second People's Hospital as an example,it explores the construc-tion of a new model of medical emergency rescue in large public hospitals,including three kinds of capabilities:the ability to transform the combination of level and emergency,the ability to integrate regional medical rescue and coor-dination,and three-dimensional multifunctional emergency rescue capabilities;and two norms:norms for talent training and norms for the management system.Finally,it puts forward suggestions for strengthening the construc-tion of emergency hospitals.

The National Conference on Medical Emergency Work in 2024 clearly puts forward the need to continu-ously strengthen the construction of emergency medical rescue system and capacity,does a good job in medical treatment of emergencies,and achieves high-quality development of medical emergency work.On the basis of ana-lysing the current situation of medical emergency rescue system construction,taking the construction practice of the first emergency hospital in China in Guangdong Second People's Hospital as an example,it explores the construc-tion of a new model of medical emergency rescue in large public hospitals,including three kinds of capabilities:the ability to transform the combination of level and emergency,the ability to integrate regional medical rescue and coor-dination,and three-dimensional multifunctional emergency rescue capabilities;and two norms:norms for talent training and norms for the management system.Finally,it puts forward suggestions for strengthening the construc-tion of emergency hospitals.