The authors investigated the incidence of stratum corneum and upper epidermal cytoplasmic antibodies with 30 untreated and 20 treated psoriasis sera, and normal human sera using normal human skins of 5 different sites and psoriatic lesion by the method of indirect immunofluorescence in order to evaluate immunologic responses i n psoreasis. The results are summarized as follows . I) The positivity of stratum corneum antibodies in untreated psoriasis sera(78.7%) was si, nificantly higher than that in normal sera(64.0%). The incidence of stratum corneum antibodies in untreated psoriasis sera was found to be the highest in the arm, followed by the scalp, leg, abdomen, and face as substrate. 2) The positivity of stratum corneum antibodies in psoriasis and normal human sera was significantly higher when tested with the psoriatic lesion as substrate than norma! skin as substrate, 3) The titer of stratum corneum antibodies in 5 sera using human skin obtained from 5 different sites on the body as substrate are the highest in the arm, and leg, and(in decreasing order of frequency) the scalp, abdomen, and face. 4) The positivity of upper epidermal cytoplhsmic antibodies in normal human sera (40.7%) was significa.ntly higher than that in untreated psoriasis sera(21.3%). 5) Ir.. the majority of cases, upper epidermal cytoplasmic antibodies coexisted with straturn corneum antibodies in the sera of patients with psoriasis and in the sera of normal humans.
Abdomen ; Antibodies* ; Arm ; Cytoplasm* ; Fluorescent Antibody Technique, Indirect ; Humans ; Humans* ; Incidence* ; Leg ; Psoriasis* ; Scalp ; Skin

No abstract available.
Adenosine Triphosphatases* ; Animals ; Langerhans Cells* ; Mice* ; Oxygen*

BACKGROUND: CD44 is a family of glycoproteins involved in cell to cell and cell to matrix interactions. Overexpression of CD44v6(variant exon 6) form has been reported in several malignant tumors such as stomach cancer, uterine cervical cancer, colorectal cancer, breast cancer and keratinocytic skin tumors, such as, basal cell carcinoma and squamous cell carcinoma. However, CD44 expression in cutaneous T cell lymphoma has not been investigated thoroughly. OBJECTIVE: The purpose of this study is to examine whether there is any difference in the expression of CD44s & CD44v6 between mycosis fungoides(MF), angiocentric T cell lymphoma, subcutaneous panniculitic T cell lymphoma, Ki lymphoma and unspecified peripheral T cell lymphoma. We also evaluated the statistical significance between the expression of CD44v6 and systemic involvement of the diseases. METHODS: Routine paraffin sections of formalin-fixed 33 tissues (11 MF, 8 angiocentric T cell lymphoma, 5 subcutaneous panniculitic T cell lymphoma, 2 Ki lymphoma, 1 unspecified peripheral T cell lymphoma, 2 psoriasis, 2 lichen planus, 2 erythema nodosum) were labeled with anti-CD44 monoclonal antibody using a avidin-biotin-peroxidase complex. Normal skin served as the negative control. RESULTS: 1. Eccrine glands, hair follicles and the epidermis, except the cornified layer, showed positive staining for CD44s. In inflammatory skin diseases, it showed positive staining for CD44s, however CD44v4/5 and CD44v6 stainings were negative. 2. All(4 out of 4) of the tumor stages of MF showed positive CD44s staining, and 3 out of 4 showed positive CD44v6 staining(p=0.024). However, none of them expressed CD44v4/5. 3. All 8 cases of angiocentric T cell lymphoma were positively stained for CD44s, but not for CD44v4/5. In contrast to other peripheral T cell lymphoma, CD44v6 was expressed in 3 out of 8 cases of angiocentric T cell lymphoma. In subcutaneous T cell lymphoma, only the CD44s was expressed, and Ki lymphoma was positively stained for CD44s and negatively stained for CD44v4/5 and CD44v6. 4. In CD44v6 positive angiocentric T cell lymphoma (3 out of 8) and CD44v6 positive tumor stages of MF (3 out of 4), 5 out of 6 patients had systemic involvement suggesting a statistical significance between CD44v6 expression and patient's systemic involvement(p=0.015). CONCLUSION: These results suggest that CD44v6 may serve as a useful prognostic marker in the tumor stage of mycosis fungoides and angiocentric T cell lymphomas.
Breast Neoplasms ; Carcinoma, Basal Cell ; Carcinoma, Squamous Cell ; Colorectal Neoplasms ; Eccrine Glands ; Epidermis ; Erythema ; Exons ; Glycoproteins ; Hair Follicle ; Humans ; Lichen Planus ; Lymphoma ; Lymphoma, T-Cell ; Lymphoma, T-Cell, Cutaneous* ; Lymphoma, T-Cell, Peripheral ; Mycosis Fungoides ; Paraffin ; Psoriasis ; Skin ; Skin Diseases ; Stomach Neoplasms ; Uterine Cervical Neoplasms

Annular elastolytic giant cell granuloma(AEGC(3) has been called as atypical necrcibiosis lipoidica, Mieschers granuloma of the face and scalp, and actinic granu- Joma and needs differen(iation from granuloma annulare, elastosis perforans serpiginosa and nerrobiosis lipoidica. We report a case of AECCG occurring in a 53-year-old male. The patient. had an annular erythematous plaque measuring 4 cm in diameter with serpiginous raised edges and slightly atrophic centers on the right forearm for 5 years. The histopathologic finding showed patchy dermal infiltration of lymphocytes, histiocytes, and multinucleated giant cells engulfing elastic fibers. The. skin lesion cleared after intrader mal injection cf cortieosteroids.
Actins ; Elastic Tissue ; Forearm ; Giant Cells* ; Granuloma ; Granuloma Annulare ; Granuloma, Giant Cell* ; Histiocytes ; Humans ; Lymphocytes ; Male ; Middle Aged ; Scalp ; Skin

We report a case of Wells' syndrome consisting chiefly of papular, vesicular, pustular and vegetative lesions on the trunk, lower extremities and palms in a 48-year-old female, though characteristic lesions of this syndrome are known to be cellulitis or urticaria like plaques. Only a few cases with such lesions as ours have been reported until now. Histologic finding showed spongiotic vesicles in the epidermis and diffuse dense infiltration of eosinophils with characteristic flame figures in the dermis. In addition, marked blood eosinophilia was noted. We tried oral prednisolone for 3 weeks, and the skin lesions improved almost completely.
Cellulitis ; Dermis ; Eosinophilia ; Eosinophils ; Epidermis ; Female ; Humans ; Lower Extremity ; Middle Aged ; Prednisolone ; Skin ; Urticaria

Dermstomyositis is a disease mainly of the skin, muscle and blood vessels in which erythematous and edematous changes in the skin are associated with muscle weakneas and inflammation. In adult the disease is commonly associated with underlying malignancy or reticulosis. A 23 year-old male had generalized weakness, intermittent fever, myalgia and purplish red patches on the face. Diagnosis of dermatomyositis was established by clinical manifestations, electromyogram, histologic findings of skin and muscle. He was treated with systemic steroid, with some improvement. Seven months after the onset of dermatomyositis, ecchymoses was noted on the eyelids and forearms. Bone marrow aspiration showed many immature lymphoblasts, characteristic of acute lymphocytic leukemia, He was treated with systemic steroid and vincristine, but no favorable effect was noted. He died of sepsis due to retal abscess on the 34th day in the hospital
Adult ; Male ; Female ; Humans

BACKGROUND: Topical 1% butenafine hydrochloride cream, a new benzylamine derivative, has been reported to have antimycotic effect. OBJECTIVE: An open trial as conducted to assess the efficacy and safety of 1% butenafine hydrochloride cream used once daily in the treatment of tinea pecis. METHODS: Of 45 patients with clinically and mycologically proertinea pedis from the department of dermatology at Kosin medical college hospital, 42 patient, who could be monitored to the end of this study were evaluated. They applied 1% butenafine hydrochloride cream once daily until clinical improvement became evident or for 4 weeks and the effects were evaluated clinically and mycologically. RESULTS: 1. From the mycological studies at the initial visit, causative organisms were isolated from 36 patients out of the 42 subjects and Trichophyton rubrum was most comnmon organism. 2. The negative conversion rate of the mycological study( negative microscopy and negative culture) was 90.5%(38/42) after 4 weeks of treatment. 3. The effective treatment vate at 4 weeks was 90.5%(38/42) 4. A adverse effects of using 1% butenafine hydrochloride cream was noted in 1 patient, who had a moderate degree of puritus, out of the 42 subjects. The presritus disappeared soon after the cessation of application of the cream. CONCLUSION: 1% butenafine lydrochloride cream is effective andafe in the treatment of tinea pedis.
Dermatology ; Humans ; Microscopy ; Tinea Pedis* ; Tinea* ; Trichophyton

The mechanism responsible for the formation of sunburn cells in mammalian skin is unknown. However it is suggested that the reactive free radicals and oxygen species generated by UV radiation are causing oxidative reactions in certain keratinocytes that are manifestated in the form of dyskeratotic cells and free radical scavengers such as a-tocopherol acetate or p-carotene were thought to be photoprotective against sunburn radiation. In the present study, we evaluated the effect of single and multiple applications of n-tocopherol acetate(a-TCA) and p-carotene on the formation of sunburn cells. We also determined the effect of these two antioxidants on the sunburn reartion by UVB irradiation. The results were as follows : 1. Both a-tocopheroJ acetate and p-carotene were photoprotective and prevented the formation of sunburn cells and sunburn reaction. 2. Topical p-carotene was less photoprotective than u-tocopherol acetate. 3. Topical use of a-tocopherol acetate and p-carotene in dose range exceeding 1,000 pgcm could provide photoprotective effect if the UVB exposure doses were less than 300 mJ/cm'( 3 MED)
Animals ; Antioxidants ; Carotenoids* ; Free Radical Scavengers ; Free Radicals ; Guinea Pigs* ; Guinea* ; Keratinocytes ; Oxygen ; Skin ; Sunburn ; Tocopherols*

The clinical and histopathologic study of 26 cases with tsutsugamushi disease seen at the Department of Derrnatology, Kosin Medical Center, Pusan, Korea from October 1986 to December 1988 was performed. The results were summarized as follows : 1. Age distribution was from 12 to 73 years and male to female ratio was 1: 1.2 (1P, males 14 females). 2. The disease occurred from September to December in a year. The peak incidence was in October (61.5%). 3. Major clinical manifestations were as follows : Chill, fever and headache (100 %), maculopapular eruption (90.2%), eschar (88.5%) and myalgia (84.6%). 4. Eschars were distributed on the abdomen (34.8%), chest (30.4%), leg (7.4%) and ot.her sites. 5. Histopathologic findings of the erythernatous macules in 24 patients showed epiderrnal changes including exocytosis of rnononuclear cells (66.7%), liquefaction degeneration of basal cells (54.2%), spongiosis (33.3%) and epidermal cell necrosis (29.2%). Dermal changes revealed vascular dilatation and perivascular infiltration of rnononuclear cells (100%), extravasated RBC (50%), and edema of papillary dermis (45.8%). Epidermal changes around the eschar were liquefaction degeneration of basal cells (52.2%), spongiosis (43.5%) and exocytosis of mononuclear cells (30.4%). Dermal changes included vascular dilatation and perivascular mononuclear cell infiltration (100%), vessel wall necrosis (73.9%), ederna of papillary dermis (65.2%) and endothelial cell swelling (65.2%).
Abdomen ; Age Distribution ; Busan ; Dermis ; Dilatation ; Edema ; Endothelial Cells ; Exocytosis ; Female ; Fever ; Headache ; Humans ; Incidence ; Korea ; Leg ; Male ; Myalgia ; Necrosis ; Scrub Typhus* ; Thorax

BACKGROUND: Previous studies have shown that in vitro exposure to ultraviolet B(UVB) radiation resulted in a dose-dependent inhibition of natural killer activity(NK activity) of normal human peripheral blood mononuclear cella(PBMC), and that in vivo exposure to snlight also induced NK activity suppression. The precise meehanism of the UV-regulation on the riat iral killer system(NK system) is not established. Objective & METHOD: The purpose of this study is to examine whether the addition of interleukin-2(IL-2) and/or free oxygen radical scavengers, superoxide dismutas(SOD) or sodium azide(SA), is effective in reducing the UVJ3-induced suppression of NK activity of FBMC. RESULTS: The results are as follows 1. The suppressive effect of UVB radiat,ion on NK activity could successfully be prevented in the presence of SOD(100 and 1,000U/ml) during the radiation. 2. SA( LO and 10 M/ml) did not prevent the suppression of NK activity. 3. IL-2(100U/ml) markedly enhanced the NK activity of nonirradiated PBMC, but had no effect on irradiated PBMC. 4. Combination treatment with both IL-2 and free radical scavengers on UVB-irradiated PBMC resulted in no additive or synergistic effect on the prevention of the suppression of NK activity compared with a single treatment with either IL-2 or free radical scavengers. CONCLUSION: In the presserit study, we found that SOD providec a protective effect on NK activity during the UVB radiation and we suggest that superoxide anion(O ) might play a major role in the UV-regulatory mechanisms of the NK system.
Free Radical Scavengers ; Humans* ; Interleukin-2* ; Oxygen ; Sodium ; Superoxides