Vimentin is an intermediate filament protein normally expressed in mesenchymal cells, but the evidence of the aberrant expression of vimentin in epithelial cancer cells suggests that the vimentin expression might be related to local invasiveness and metastatic potential. There have been a few previous studies on the vimentin expression in human cervical carcinogenesis using in vivo and in vitro models. We examined the immunohistochemical vimentin expression in various squamous epithelial neoplasms of the uterine cervix, including the cervical intraepithelial neoplasia group (n=25), the microinvasive squamous cell carcinoma group (n=15), the invasive squamous cell carcinoma group (n=15) and the metastatic squamous cell carcinoma group (n=8). Vimentin positivity was significantly higher in the invasive than in the intraepithelial group, and in the cases with lymph node metastasis than in those without metastasis, suggesting a relationship between the vimentin expression and progression of the uterine cervical epithelial tumors.
Carcinogenesis ; Carcinoma, Squamous Cell ; Cervical Intraepithelial Neoplasia ; Cervix Uteri* ; Female ; Humans ; Intermediate Filaments ; Lymph Nodes ; Neoplasm Metastasis ; Neoplasms, Glandular and Epithelial* ; Vimentin*

This study was undertaken to investigate the effect of UVB radiation on epidermal melanoctes of C57BIL mouse, The results are summarized as follows : l) The numerical increase of DOPA positive melanocytes following UVB irradiation was due to both of active mitosis of epidermal melanocytes and the conversion of dormant. malanocytes to DOPA-positive melsnocytes. 2) Long term effects of UVB irradiatinn on the epidermal melanocytes suggest the persistent activation of typrosinase in UVB irradiated melanocytes.
Animals ; Dihydroxyphenylalanine ; Melanocytes* ; Mice* ; Microscopy, Electron ; Mitosis

No abstract available.
Arthroplasty* ; Arthroplasty, Replacement, Hip*

The Kniest syndrome is characterized by disproportionate dwarfism and Kyphoscoliosis which may be associated with flat facies with prominent eyes, cleft palate, hearing loss, myopia and limited joint motion. The skeletal abnormalities are recognizable at birth with shortening and deformity of the extremities and stiff joints. Marked lumbar lordosis and kyphoscoliosis develop in childhood, resulting in disproportionate shortening of the trunk. We experienced a case of kniest syndrome, confirmed by clinical features, radiological features, and histological examination of cartilage. A brief review of the related literature is presented.
Animals ; Cartilage ; Cleft Palate ; Congenital Abnormalities ; Dwarfism ; Extremities ; Facies ; Hearing Loss ; Joints ; Lordosis ; Myopia ; Parturition

No abstract available.
Animals ; Pertussis Vaccine* ; Rats* ; Streptozocin* ; Whooping Cough*

No abstract available.
Leptospira interrogans* ; Leptospira*

This study was designed to investigate the effects on count, size, and morphology of human corneocytes when the skin is irritated with a rough towel. The desquamating portion of the stratum corneum was sampled with the detergent scrub technique every other day for 12 days. The following parameters were measured; numerical count, size (surface (mu)2), and shape (regular, irregular). Corneocytes from skin irritated with a rough towel differed from those of normal skin in that they were diminished by about 60% in count and were 14% smaller in size on the first experimental day. These parameters became normalized in count after 10 days and in size after 5 days respectively. On the morphological classification of the cell outlines, there were no significant differences from the control groupts except that there were slightly increased numbers of irregular cells in the experimental groups.
Adult ; Baths* ; Bedding and Linens* ; Epidermis/cytology* ; Human ; Male

BACKGROUND: Long-term phototherapy can induce the changes of photoaging and it is reported that there is an increased chance of cutaneous squamous cell carcinomas in patients exposed to large amounts of UV radiation. OBJECTIVE: The main objective was to investigate the degree of photoaging and the presence of p53 mutations in normal skin in patients undergoing long-term phototherapy. METHOD: We performed hematoxylin-eosin and special stains, p53 and p21 immunohistochemical stains and polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) on the normal skin of patients subject to long-term UV therapy. RESULT: 1. The typical features of photoaging were not observed in patients undergoing long-term UV therapy. 2. In p53 immunohistochemical staining performed at 1 week after cessation of long-term PUVA treatment, the patient group with a culmulated UV dosage of more than 1,000J/cm2 demonstrated an increased number of p53 positive epidermal cells compared to exposed as well as unexposed normal skins. 3. The patterns of p21 immunohistochemical staining performed at 1 week after cessation of long-term PUVA and UVB treatments were similar to that of p53 immunohistochemical staining performed at 1 week after cessation of phototherapy. 4. In p53 immunohistochemical staining performed at 4 months after cessation of UV treatment, the number of p53 positive epidermal cells decreased significantly compared to that of p53 positive epidermal cells found at 1 week after cessation of UV treatment. 5. The mutation of p53 genes was not found in PCR-SSCP analysis of biopsied skins done at 1 week after cessation of long-term PUVA and UVB treatment. CONCLUSION: Long-term phototherapy did not induce the typical changes of photoaging and p53 overexpression in the epidermis of UV treated skin was a reactive process. Therefore, UV therapy can be a relatively safe treatment modality, although a closer observation for cutaneous malignancy is warrented in the patients whose cumulated UV dosage is much higher than 1,000J/cm2.
Aging* ; Carcinoma, Squamous Cell ; Coloring Agents ; Epidermis ; Genes, p53 ; Humans* ; Phototherapy ; Skin*

No abstract available.
Fibroma, Desmoplastic* ; Fibula*

Arthrodesis of the knes has lost its nopularity since the development of modern knee replscement arthroplasties, however it still has its place as a salvage procedure when replacement arthroplasties are contraindicated. Various techniques have been in the use including the time-tested Charnleys compressiori arthrodesis. Instead of using Charnley's and other similar compression devices, the authors applied single or double compression plates in 8 knees with severe destruction and deformities, and the results are summerized ss follows: l. Included in this series are 5 cases of tuberculous arthritis, 1 case of septic arthritis, 1 case of resistant scar contracture, and 1 case of Charcots knee. 2. Successful fusion was obtained within 18th week, aversge 13th week. No case of pseudarthrosis was experienced. 3. 3 cases of postop. wound drainage and 1 case of peroneal nerve palsy were experienced, but these did not compromise the final results. 4. It may be concluded that Arthrodesis of the knee using compression plate is rigid enough to permit early mobilization of patients and get the certainty of union.
Arthritis ; Arthritis, Infectious ; Arthrodesis ; Arthroplasty ; Arthroplasty, Replacement ; Cicatrix ; Congenital Abnormalities ; Contracture ; Drainage ; Early Ambulation ; Humans ; Knee ; Paralysis ; Peroneal Nerve ; Pseudarthrosis ; Wounds and Injuries